An Intuitive Detection Method Based on Polarized Light Microscopy for Adsorbed Lipids on Montmorillonite

A detection method and montmorillonite technology, applied in the field of medicine, can solve the problem that the characteristic detection method of lipid adsorption has not yet been reported, etc.

Active Publication Date: 2018-01-05
BEIJING NORMAL UNIVERSITY
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  • Claims
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Problems solved by technology

[0004] The existing patent-modified montmorillonite capable of adsorbing zearalenone and its preparation method (patent number: 2012101647306) shows that montmorillonite can adsorb zearalenone, but all of them are through complicated laboratory detection methods , such as X-ray diffraction, Fourier transform infrared analysis, scanning electron microscope analysis and various adsorption tests for identification, but its characteristic detection method for lipid adsorption has not been reported

Method used

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  • An Intuitive Detection Method Based on Polarized Light Microscopy for Adsorbed Lipids on Montmorillonite
  • An Intuitive Detection Method Based on Polarized Light Microscopy for Adsorbed Lipids on Montmorillonite
  • An Intuitive Detection Method Based on Polarized Light Microscopy for Adsorbed Lipids on Montmorillonite

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Embodiment 1

[0023] The detection method based on polarized light microscope observation of lipid in montmorillonite adsorption of high-fat food or feed mainly includes the following steps:

[0024] 1. Sample preparation: Mix montmorillonite, high-fat food or feed, and the two to make smears, stain with Oil Red O, and then make slide samples for observation under a microscope; Determine the number of samples to be tested according to the corresponding standards.

[0025] 2. Polarized light microscope detection: place the prepared sample on the stage, obtain a clear image under bright field, and use the digital equipment matched with the microscope to obtain the image and save it in the computer. Adjust the polarized light microscope so that the polarizer and analyzer are orthogonal. Insert the compensation sheet, adjust the polarizer or analyzer to an appropriate angle to obtain a clear and bright image, and use the digital equipment matched with the microscope to obtain the image and sav...

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Abstract

The invention relates to an intuitive detection method based on polarized light microscope observation of montmorillonite adsorbed lipids. First, montmorillonite is mixed with high-fat food or feed, and then the sample is made into a smear, and the neutral lipid dye oil Red 0 was used to stain the smear, and the prepared smear was detected under a polarized light microscope. Observe the adsorption properties of montmorillonite to lipids in high-fat food. The invention is a physical detection method, which can directly observe the adsorption of montmorillonite to lipid, and is simple in operation, intuitive and convenient.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to an intuitive detection method for observing montmorillonite-adsorbed lipids based on a polarized light microscope. Background technique [0002] In recent years, various types of purified montmorillonite minerals have been widely used in many fields such as medicine and hygiene, food, feed and cosmetics. Gastrointestinal antidiarrheal drugs with montmorillonite as the main component, such as montmorillonite powder (Smecta), have been recognized as OTC drugs and are widely used in the treatment of diarrhea or other gastrointestinal diseases. Described as an intestinal mucosal protective agent, it can fix and inhibit viruses, bacteria and the toxins produced in the digestive tract; it has the ability to cover the mucosa of the digestive tract, and can repair and improve the mucosal barrier by combining with mucus glycoproteins. The defense function of the attacking factor. Feed mo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/21
Inventor 翟永功许鹏飞戴书
Owner BEIJING NORMAL UNIVERSITY
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