Establishment and application of rhesus monkey pulmonary fibrosis model

A technology for pulmonary fibrosis and rhesus monkeys, which can be applied to respiratory diseases, glycopeptide components, drug combinations, etc., can solve the problems that no rhesus monkey pulmonary fibrosis model has been established, and has good clinical application prospects and is easy to repeat , The effect of simple operation process

Active Publication Date: 2015-12-09
WEST CHINA HOSPITAL SICHUAN UNIV +1
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

Since the current pulmonary fibrosis treatment drugs and methods cannot effectively control the spread of fibrosis, there is an urgent need to establish a non-human primate pulmonary fibrosis model to provide evaluation for new drugs and targeted therapies
[0004] Currently, there is no report on the establishment of a rhesus monkey model of pulmonary fibrosis

Method used

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  • Establishment and application of rhesus monkey pulmonary fibrosis model
  • Establishment and application of rhesus monkey pulmonary fibrosis model

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Experimental program
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Embodiment 1

[0023] The preparation of embodiment 1 animal model of the present invention

[0024] 1. Materials and methods

[0025] 1.1 Experimental materials

[0026] Six 2-3-year-old male rhesus monkeys, free from B-herpes virus, simian retrovirus, simian leukemia virus and immunodeficiency virus infection: purchased from Meishan Animal Breeding Base.

[0027] 1.2 Main reagents

[0028] Bleomycin (BLM) was purchased from Beijing Baidekang Pharmacy and produced by Nippon Kayaku Co., Ltd.

[0029] Other medicines and consumables were purchased from Amity Technology.

[0030] 1.3 Main solution configuration

[0031] Configuration of bleomycin saline solution

[0032] Take 15 mg titer of bleomycin per bottle, inject 200 microliters of normal saline, dissolve to form 75 mg / ml bleomycin titer

[0033] 1.4 Main Instruments

[0034] 1 high pressure atomizing syringe: PennCentury

[0035] 2 Anesthesia machine: Excel210se

[0036] 3CT machine: GEBRIVOCT325

[0037] 4 Anesthesia laryngos...

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Abstract

The invention discloses a method for preparing an animal pulmonary fibrosis model. According to the method, bleomycin is applied to a primate, the application dose of the bleomycin is 7 mg/Kg for each time, and application is performed once for each week and totally performed twice. The invention further provides a rhesus monkey pulmonary fibrosis model prepared with the method and an application of the rhesus monkey pulmonary fibrosis model. The rhesus monkey pulmonary fibrosis model is established successfully, an operation process is simple and easy to repeat, and the model can be used for new biotechnological drug evaluation which cannot be finished by a rodent model and has bright clinical application prospects.

Description

technical field [0001] The invention relates to a preparation method and application of a rhesus monkey pulmonary fibrosis model. Background technique [0002] Pulmonary fibrosis is a major disease that affects human health. The cause of the disease is unknown. So far, there is no effective prevention and treatment. Although the commonly used mechanical ventilation and hormone therapy can alleviate the patient's dyspnea and hypoxemia to a certain extent, long-term use of hormone therapy may induce other diseases, and the cost of mechanical ventilation is high, and the operation is not easy. None of the above treatment methods can fundamentally cure pulmonary fibrosis, nor improve the survival rate. How to prevent the spread of pulmonary fibrosis, effectively inhibit the growth of fibroblasts in the lung, and fundamentally block fibrosis is the key point of current research [0003] The most commonly used model for pulmonary fibrosis research today is the rodent. Because r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/14A61P11/00
Inventor 刘潋文富强陈磊钟治晖汪涛王浩袁智城陈梅
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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