Enhanced transgene expression and processing

Abstract

Disclosed are constructs and methods for expressing DNAs of interest in particular in non- primate eukaryotic host cells that display advantages with regard quantity and quality of expression including high stability of expression and, if appropriate, transport of the expression product out of the cell.

Description

technical field [0001] The present invention relates to the provision of nucleic acid constructs and proteins involved in or acting on metabolic pathways that mediate or affect cellular metabolism, such as translocation across the ER membrane and / or translocation across the plasma membrane, and methods of affecting cellular metabolism. secretion. The invention also relates to the preparation and use of recombinant mammalian cells in which, for example, the translocation / secretion of diverse heterologous proteins (transgene expression products) is altered. The methods, nucleic acid constructs are generally designed to enhance transgene expression. Background technique [0002] The biotechnological production of therapeutic proteins and gene and cell therapies depends on the introduction of successful transgene expression into eukaryotic cells. Successful transgene expression generally requires integration of the transgene into the host chromosome and is particularly limited...

AI Technical Summary

Problems solved by technology

However, for example, Lakkaraju et al. (2008) reported that expression of exogenous SRP14 in wild-type human cells (e.g., in cells that have not been engineered to express low levels of SRP14) did not enhance secretion of the secreted alkaline phosphatase protein efficiency

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