Enhanced transgene expression and processing

A transgenic and gene technology, applied in the field of recombinant mammalian cell preparation, can solve the problem of not improving the efficiency of alkaline phosphatase protein secretion and the like

A transgenic and gene technology, applied in the field of recombinant mammalian cell preparation, can solve the problem of not improving the efficiency of alkaline phosphatase protein secretion and the like

CN105073995APending Publication Date: 2015-11-18SELEXIS SA
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  • Enhanced transgene expression and processing
  • Enhanced transgene expression and processing
  • Enhanced transgene expression and processing

Examples

Experimental program
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Effect test

Embodiment Construction

[0165] A transgene as used in the context of the present invention is an isolated isolated functional RNA (non-coding RNA) that encodes a given mature protein (also referred to herein as protein-encoding DNA), a precursor protein, or does not encode a protein. Deoxyribonucleotide (DNA) sequence. The transgene is isolated and introduced into cells to produce a transgene product. Some preferred transgenes according to the invention are those encoding immunoglobulin (Ig) and Fc fusion proteins and other proteins, especially proteins with therapeutic activity ("biotherapeutics"). For example, certain immunoglobulins such as Infliximab (Remicade) or other secreted proteins such as coagulation factor VIII are particularly difficult to express due to largely uncharacterized cellular bottlenecks. These bottlenecks can be identified and / or opened with the aid of the recombinant nucleic acid molecules, vectors and methods of the invention. This generally increases the amount of therap...

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Abstract

Disclosed are constructs and methods for expressing DNAs of interest in particular in non- primate eukaryotic host cells that display advantages with regard quantity and quality of expression including high stability of expression and, if appropriate, transport of the expression product out of the cell.

Description

technical field [0001] The present invention relates to the provision of nucleic acid constructs and proteins involved in or acting on metabolic pathways that mediate or affect cellular metabolism, such as translocation across the ER membrane and / or translocation across the plasma membrane, and methods of affecting cellular metabolism. secretion. The invention also relates to the preparation and use of recombinant mammalian cells in which, for example, the translocation / secretion of diverse heterologous proteins (transgene expression products) is altered. The methods, nucleic acid constructs are generally designed to enhance transgene expression. Background technique [0002] The biotechnological production of therapeutic proteins and gene and cell therapies depends on the introduction of successful transgene expression into eukaryotic cells. Successful transgene expression generally requires integration of the transgene into the host chromosome and is particularly limited...

Claims

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Application Information

Patent Timeline
18 Nov 2015
Publication
CN105073995A
IPC
C12N15/90; C12N15/85; C07K16/00
CPC
C12N15/85; C07K16/00; C07K16/241; C07K16/32; C12N15/90; C12N2800/107; C12N2800/90; C12N2830/46
Inventors
V·勒富尔; N·梅尔莫