A method of inducing young primates to obtain gonad gametes and applications thereof
A primate and juvenile technology, applied in the biological field, can solve the problems of unrecoverable, unrepeatable, and long-term damage to young monkeys, and achieve the effects of shortening the generation cycle, saving time, and reducing production costs
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[0034] Example 1
[0035] Gonadotropin-releasing hormone analogues (Gonarelin) combined with human follicle-stimulating hormone and human chorionic gonadotropin induce young female monkeys to acquire gonadal gametes.
[0036] 1. Take a 1-year-old female cynomolgus monkey, weighing 1.0 kg, and has not had any symptoms of premature sexual maturity, such as plasma estradiol level at 20ng / mL, and no abnormalities in other physiological aspects.
[0037] 2. Daily injection of 28.8 micrograms of gonarelin, dissolve it in normal saline and divide it into 288 equal parts, subcutaneously inject once every 5 minutes for 60 days.
[0038] 3. After starting the injection of gonarelin, blood is drawn once a week to test the plasma estrogen level. It can be observed that the skin of the baby monkeys gradually increases and the color gradually changes to bright red, indicating that the plasma estrogen level is elevated and the female is young. Monkeys gradually reach sexual maturity. The concentrat...
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[0043] Example 2
[0044] Human chorionic gonadotropin combined with gonadotropin releasing hormone analogue (Gonarelin) induces the acquisition of gonadal gametes in young male cynomolgus monkeys.
[0045] 1. Take a 2-year-old male cynomolgus monkey, weighing 1.6 kg, without any symptoms of premature sexual maturity, such as plasma testosterone level less than 1.0ng / mL, and no abnormalities in other physiological aspects.
[0046] 2. Inject 160IU horse chorionic gonadotropin once a day for 15 days. Blood was drawn one week before the start of horse chorionic gonadotropin and once a week after the injection, and the plasma testosterone level reached 5.0 ng / mL.
[0047] 3. Then began to inject 60μg of gonadotropin-releasing hormone analogue gonarelin every day, dissolve it in normal saline, divide it into six equal parts, and continuously inject 6 times intramuscularly every 3 hours every day for 90 days.
[0048] 4. Continue to give the same dose and the same method of gonarelin in ste...
Example Embodiment
[0051] Example 3
[0052] Human chorionic gonadotropin combined with gonadotropin releasing hormone analogue (Gonarelin) induces the acquisition of gonadal gametes in young male cynomolgus monkeys.
[0053] 1. Take a 3-year-old male cynomolgus monkey 3.5 kg, without any symptoms of premature sexual maturity, such as plasma testosterone level less than 1.0ng / mL, and no abnormalities in other physiological aspects.
[0054] 2. Inject 1750IU equine chorionic gonadotropin once a week for 3 weeks. Blood was drawn one week before the start of horse chorionic gonadotropin and once a week after the injection, and the plasma testosterone level was detected to be above 10.0ng / mL.
[0055] 3. Then start to inject 80μg of gonarelin every day, dissolve it in normal saline, divide it into 4 equal parts, and give 4 continuous intramuscular injections every 6 hours every day for 15 days.
[0056] 4. Continue to give the same dose and the same method of gonarelin in step 3, and at the same time use art...
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