A method of inducing young primates to obtain gonad gametes and applications thereof

Abstract

A method of inducing young primates to obtain gonad gametes is disclosed. The method includes administering a female young primate with gonadotropin-releasing hormone or an analogue thereof, performing combined administration of gonadotropin, and inducing follicular development to obtain mature oocytes that are female gametes; administering a male young primate with gonadotropin, performing combined administration of gonadotropin-releasing hormone or an analogue thereof, and inducing testis spermatogenesis to obtain sperms that are female gametes; and subjecting the obtained female gametes and male gametes to fertilization, and allowing fertilized eggs to develop until blastulas or a filial generation. A hormone combination is adopted by the method to promote induction of young primate sexual prematurity, thus obtaining the gonad gametes in advance, significantly shortening the generation period of primates, and accelerating offspring breeding. The method can achieve large-scale expanding propagation of the young primates, shorten the generation period, reduce the cost, and provide experience references for germplasm preservation of precious and endangered animal species and rapid animal propagation through gene editing.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to a method and application for inducing young primates to obtain gonadal gametes. Background technique [0002] As we all know, the genetic information of a species will be lost with its extinction. Today, with the increasing number of endangered animals, how to safely and effectively obtain gonadal gametes from young endangered animals and then obtain test-tube animals is a major problem in saving endangered animals. [0003] Primates have high gene homology and similar phylogeny with humans, making them excellent experimental models for biomedical research. In recent years, more and more attention has been paid to major human diseases caused by gene mutations, and primate transgenic models have also emerged as the times require. With the continuous innovation of gene editing technology, gene editing has become easier and more efficient. However, the obtained transge...

AI Technical Summary

Problems solved by technology

[0005] At present, the only method for obtaining sexually derived gametes from young primates is xenotransplantation of testicular tissue (Liu et al., Generation of macaques with sperm derived from juvenile monkeytesticular xenografts[J]. Cell Research, 2016, 26(1) :139-142), but this method has great defects: (1) firstly, the young monkey needs to be orchiectomized, and the damage to the young monkey cannot be recovered and cannot be repeated; (2) it takes a long time, and the transplantation takes 12 months (3) The age of the monkey that provided the testicular tissue was older

At present, how to obtain gonadal gametes of young animals in a relatively short period of time, and then obtain their blastocysts or offspring without causing harm to primates has not yet been reported.

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