204 results about "Female gonad" patented technology

The invention relates to a method for culturing river crabs, in particular to a method, by which the river crabs cultured in a pond or other water areas and become large-size commercial crabs. The method is characterized in that the method comprises the steps of purchasing crab larvae; culturing the crab larvae: the disinfection of the crab larvae incubating pond, the arrangement of the escape preventing fence, fertilization, water injection to the crab larvae pond, the float grass cultivation, the stocking of the crab larva, and the feeding of artificial food; the over-winter of first instar crab; the culturing of the second instar crab: the disinfection of the crab larvae incubating pond, the arrangement of the escape preventing fence, fertilization, water injection to the crab larvae pond, the float grass cultivation, the feeding of the first instar crab, and the feeding of artificial food; controlling the gonad development speed of the second instar crab: the artificial over-summer and the natural over-winter of the second instar crab; culturing the large-size river crabs: the feeding and management for the second instar crab, the artificial induction to the river crabs for exuviation and growth. The method is in favor of increasing the culture scale, the culturing yield and the economic benefit through controlling the gonad development speed of the second instar crabs and applying the technologies of culturing the large-size river crabs.

A process for polycross breeding a Manila clam pedigree, includes: providing present Manila clam pedigrees of different shell color types as parents; ecologically maturing the parents; reciprocally hybridizing the parents by every two of different shell color types to obtain offsprings thereof; nurturing the offsprings until gonads thereof mature synchronously; and hybridizing the matured offsprings with each other to obtain at least a four-way cross pedigree accumulating all valuable genes of the parents.

The invention relates to a method for comprehensively regulating and controlling gonad mature ovulation of cheilinus undulates, which comprises the following steps: preferably selecting, breeding and feeding parent fishes; regulating and controlling water temperature, illumination and water flow to enable the parent fishes to grow; and enhancing the nutrition to promote gonad mature ovulation. The technical method is used for screening artificial ripening effective temperature, intensity of illumination and water flow velocity factor data, screening varieties and doses of artificial ripening effective nutrition enhancers, screening the time suitable for gametogenesis and fertilization of cheilinus undulates, and acquiring high-quality fertilized eggs of cheilinus undulates. The fertilization rate of the eggs of the cheilinus undulates acquired by the method is 20-86%, and the hatching rate is 30-78%. The technical method for comprehensively regulating and controlling gonad mature ovulation of cheilinus undulates, which is provided by the invention, has the characteristics of advancement, high efficiency, practicality and reliability and comprehensively regulates and controls temperature, illumination, water flow and nutrition enhancement in the breeding process of seedlings of cheilinus undulates. The invention has great application value and wide popularization and application prospects for protecting endangered species.

The invention relates to a large-scale method for raising seedlings of freshwater shrimps, which mainly comprises the following steps: the mixture ratio of female shrimps to male shrimps is between 3 and 5 to 1, and the shrimps come from different places, are more than 4.5 centimeters, have mature gonad, are able-bodied and scot-free, and have sensitive reaction to external stimulus; or shrimps with eggs and more than 5 centimeters are directly purchased in the mating period, taken as parent strains and placed into a seedling pool; high-quality full-rate mixed feed is thrown and fed from the next day; when zoeas occur in the pool, soybean milk is splashed; when shrimp larvaes begin to be abnormal, powdery mixed feed is fed when the soybean milk is splashed simultaneously; and when the proportion of abnormal seedlings is improved, the proportion of the powdery mixed feed is gradually increased and the powdery mixed feed can be completely thrown and fed after 10 days. Moreover, the transparency of a water body is controlled to between 20 and 30 centimeters; the pH value is between 7.5 and 8.5; and the dissolved oxygen is more than 5 milligrams per liter. The method is a scientific, reasonable, low-investment, high-efficiency and large-scale method for raising the seedlings of the freshwater shrimps, can effectively prevent varieties from degeneration, and is favorable for promoting good freshwater shrimps and improving the economic benefit of cultivation.

The invention discloses a method for comprehensively regulating and controlling verasper moseri gonad maturity and ovulation by using temperature, illumination and nutrition. The method comprises the following steps: regulating and controlling synchronous development of parent fish gonads by using temperature and illumination in 2 to 3 mouths before a breeding season begins; adding additives such as lecithin and the like into baits to promote nutrition absorption of parent fish when the parent fish gonad development is started; and increasing fed clam worms every day to promote final maturity and quality promotion of oocytes after the parent fish gonads are developed to a III period. The ovulating parent fish rate under the regulation and control of the method is up to 85 percent, and the spawning effect is good. The method can be used for scale production of offspring seeds; and by using the method, mass good-quality germ cells can be obtained, and smooth operation of offspring seed breeding can be ensured.

The invention discloses a method for promoting fish gonad maturity, which employs comprehensive adjustment of water temperature adjustment, light emitting adjustment and nutrition adjustment and technology preventing clit insect. The gonad of fish cultured from late February to early March and adjusted with said comprehensive adjustment matures more than one month earlier than that of in natural condition, and gonad of parent fish cultured in the middle October and adjusted with said comprehensive adjustment is about four months earlier than that of in natural condition, which advances fish breeding time. The fecundity is increase in Zhejiang province, the quality of ovum and sperm is improved, the fertility rate is 62.5%, rate of hatchability is 60.1%, and seeding survival rate is 15%.

The invention provides an odontobutis yaluensis breeding method. In the method, gonad developing maturation of parent fish is basically consistent through prenatal breeding; the parent fish group is collected to lay eggs through flowing water stimulation, oxytocic medicament injection and other methods, so that the induced spawning rate and the fertility rate are improved; more than 90 percent of fertilized eggs are incubated in an artificial egg collector under protection of the parent fish by utilizing the characteristic that the parent fish protects the eggs, so that the artificial operation strength is reduced, the environmental requirement is reduced, and the incubation rate is remarkably improved. The whole technical process is applied in a controllable range and is hardly influenced by external environment, the operation is convenient and reliable, scale production is convenient to organize, and the problem of producing the odontobutis yaluensis seedling in scale is well solved. By applying the technology, the induced spawning rate of the odontobutis yaluensis is over 80 percent, the fertility rate is over 80 percent, the hatchability is over 80 percent, and the seedling survival rate is over 70 percent.

The invention relates to a method for culturing river crabs by utilizing the early breeding crab larvae, in particular to a method, by which the river crabs are cultured in a pond or other water areas and become large-size commercial crabs. The method is characterized in that the method comprises the steps of (1) purchasing the early breeding crab larvae: the selection of the crab larvae purchasing date, the selection of the crab larvae quality, and the transportation of the crab larvae; (2) culturing the early culturing crab larvae: the disinfection of the culture pond, the arrangement of the escape preventing fence, fertilization, water injection to the crab larvae pond, the cultivation of water grass, the stocking of the early breeding crab larva, and the feeding of artificial food; (3) controlling the gonad development speed of crabs: the crabs getting through the summer artificially, the crabs getting through the winter and grow naturally, the stocking of the crabs, the management of the crab larvae getting through the winter, the promotion of the river crab for exuviation and growth, and the culture and the harvesting of the river crab. The crabs, the gonad of which is immature are cultured by utilizing the early breeding crab larvae produced at the beginning of December to the end of the April in the ensuing year; the growing period of the early breeding crab larvae can be prolonged, and the sexual precocity can not happen; through the artificial over-summer and the natural over-winter technologies, the early breeding crab larvae can become the crabs for the river crab culture; the crabs cultured by utilizing the early breeding crab larva can be cultured into the grown crab with the specification being 200 to 400g for each.

The invention belongs to the technical field of fish reproduction and relates to a method for ultralow-temperature cryopreservation of semen of siniperca scherzeri and artificial insemination in crossbreeding, which can be widely applied in culture of siniperca chuatsi in families and groups, especially large-scale culture and reproduction. The method comprises the steps of semen collection, diluent preparation, semen dilution, semen freezing, semen unfreezing, artificial insemination and the like. In the artificial insemination, as the optimum ratio of frozen sperms to fresh spawn is determined to be 1000000:1 by different experiments about sperm-spawn ratio. In the invention, the requirement for equipment is low, the operation is easy to realize, the method can be used for common fish reproduction fields, can realize storage of siniperca scherzeri resources, solves the problem of asynchronous growth of gonads of the siniperca chuatsi and the siniperca scherzeri in crossbreeding of siniperca, and increases the utilization ratio of the sperms of the siniperca scherzeri, and in the reproduction process, the sperm-spawn ratio can be flexibly adjusted according to the breeding requirements, the production need and the amount of parent fishes.

The invention relates to a male trait induction method for pelodiscus sinensis in embryonic periods. The male trait inducer is characterized in that through dissolving an aromatase inhibitor and an adjuvant into alcohol with the purity of 92-98%, the inducer with an aromatase inhibitor concentration of 50-150 mu g/mu l and an adjuvant concentration of 50-150 mu g/mu l is obtained; and the adjuvant is selected from testosterone, methyltestosterone or testosterone propionate. According to the induction method provided by the invention, the male induction rate can be more than 95% just through spraying the inducer two times at the sex determination key time point in embryonic development, and meanwhile, the normal development and low drug residues of pelodiscus sinensis gonads are ensured; and the induction method provided by the invention is easy to operate, low in cost, and remarkable in economic benefit, thereby being a sex control technology which is worthy of popularization in the existing pelodiscus sinensis breeding.

The invention relates to an artificial induced spawning method of Glyptosternum maculatum Regan originally produced in Yarlung Zangbo river in Tibet. The invention discloses an artificial induced spawning method of the Glyptosternum maculatum Regan. By adopting the exogenous induced-spawning medicine, the gonads of the naturally matured parent Glyptosternum maculatum Regans are promoted to further develop and breed by using the artificial induced spawning method so as to obtain fertilized eggs. The method comprises the following steps: (1) the mature parent Glyptosternum maculatum Regans selected and are matched according to the number ratio of 2-3:1 of the male parent Glyptosternum maculatum Regan and the female parent Glyptosternum maculatum Regan; (2) the induced-spawning medicine composition is prepared, and the dosage of the induced-spawning medicine composition for the female parent Glyptosternum maculatum Regan is 2mg domperidone maleate/kg (weight) + 10-15mg luteotropin releasing hormone d-ala analog/kg (weight) + 3-5mg carp pituitary/kg (weight); the dosage of the induced-spawning medicine composition for the male parent Glyptosternum maculatum Regan is one seconds of that of the induced-spawning medicine composition for the female parent Glyptosternum maculatum Regan; (3) the induced-spawning medicine is prepared to be pasty according to the formula; and (4) an administration method of injection by two needles is provided. The effect time of the prepared induced-spawning medicine composition for the male parent Glyptosternum maculatum Regan and the female parent Glyptosternum maculatum Regan is 10-12h; and the method can achieve the induced spawning ratio of 85 percent.

The invention relates to a method for inducing the spawning of the parent Solen grandis and collecting fertilized eggs. The method comprises the following steps: the parent Solen grandis which has smooth surface of shells, no mechanical damage on feet and appearance, healthy body and high activity and also has the 8-11cm length of shells is collected to perform maturation and temporary cultivation in an induced spawning pond; when the meat condition of the parent Solen grandis is up to more than 85% and the genital gland is dissected to find that sperms/eggs can flow by gravity, the induced spawning can be performed, the liquid Isochrysis galbana extract is used to cover the entire bottom surface of the pond in the induced spawning process to stimulate the parent Solen grandis for 20-25min; when spawning is performed and the density of fertilized eggs in the induced spawning pond is up to 10-15 per milliliter, and the siphon principle is utilized to fast move the fertilized eggs to a culture pond to culture. The method of the invention has the advantages that the utilization rate of the parent Solen grandis is high, the induced spawning effect is good, the fertilized eggs are convenient and fast to collect, the fertilization rate is up to 72%, the breeding survival rate is high, etc. Only a small number of parent oysters can be used so as to obtain enough fertilized eggs for artificial breeding, thus the method has good application prospect.

The invention relates to a method for inducing gonad maturity and ovulation of a verasper variegates parent fish by implanting a slow-release hormone, which comprises two aspects of cultivating an optimally selected parent fish and adjusting temperature and light to promote the gonad development of the parent fish, and implanting the slow-release hormone for promoting a metrocyte of the parent fish to reach final maturity and ovulation. In the invention, the slow-release hormone is a salmon gonadotropin-releasing hormone analog (sGnRHa) and is coated with a safe and high-efficiency polymer slow-release transitive system (95 percent of cholesterol and 5 percent of cellulose); a female parent fish with gonad in fourth stage is selected by using a living body sampling method or a light table method; a special implanting gun is used for implanting in back muscles, and the effect time of the slow-release hormone lasts for 14-18 days. The method can enable the metrocyte of the verasper variegates parent fish to achieve final maturity and ovulation, has low duress on parent fish reproduction, high rate of parent fish survival and gonad maturity, high fertility rate being 72 percent and high hatching rate being 56.2 percent, and can acquire a bulk of high-quality oosperms. The invention is a set of complete control technique method, can stably regulate and control the development, the maturity and the ovulation of the gonad of the verasper variegates parent fish, and acquire high-quality oosperms.

The invention belongs to field of fish breeding and aquaculture, which in detail relates to a method of artificial crossing with summer-bothus and tooth-Bothus and breeding. It comprises following steps: (1) taking summer-bothus as male parent and tooth-bothus as female parent, reinforcing the nutrient and environmental condition to promote simultaneous gonadal maturity, getting mature sperm and ovum; (2) crossing fertilized ovum and hatching; (3) culturing the hybrid body and young fish; (4) providing systematic bait in culturing period. The invention not only provides material for deseription improvement for bothus and soles, but also the lies foundation of adding a new breeding species for sea fish cultivating.

The invention relates to an artificial forced mature method to girth rockfishes, characterized in that adding yeast cell wall, mixture with abio salinous, phytic acid enzyme and methyl testis ketone, etc, into bait to force male parent fish to mature in web box on sea and enhanced feed in doors' pool, then by injecting DOM and ecology stimulus forces parent fish gonad to mature and oviposit naturally. This invention can achieve artificial control on parent fish sex of girth rockfishes with at least 70% triumph rate on sex adjustment. Meanwhile young functional male fish gained can be also used to production, which makes more than 50% parent fish come to sex mature and oviposit naturally and obtains mass high class gamete, thereby improve utilization rate of parent fish and productive capacity.

The invention relates to a patinopecten yessoensis ternary cross breeding method. Firstly the artificial cultivation accelerated ripening is implemented for the Russian patinopecten yessoensis. Secondly the artificial cultivation accelerated ripening is implemented for the Chinese patinopecten yessoensis. At the same time, the gonad maturation is achieved. A heating mode is utilized to the above patinopecten yessoensis for induced spawning, and the sperms and the eggs are respectively collected. The hybridization is implemented between the Chinese female scallop eggs and the Russian male scallop sperms or between the Chinese male scallop sperms and the Russian female scallop eggs, thus the first filial generation Chinese-Russian or Russian-Chinese two sorts of patinopecten yessoensis colonies which can be cultivated within two years with the conventional suspended longline farming method. The Janpanese patinopecten yessoensis and the Chinese-Russian or the Russian-Chinese patinopecten yessoensis are taken as the parents for the artificial cultivation accelerated ripening. At the same time, the gonad maturation is achieved. A heating mode is utilized to stimulate the induced spawning, and the sperms and the eggs collected are respectively selected for the implementation of hybridization. Thereby the multiple Russian-Chinese-Japanese, Japanese-Chinese-Russian or Janpanese-Russian-Chinese patinopecten yessoensis colonies are obtained, which can be cultivated with the conventional suspended longline farming method. The method is simple, practical with low cost. The patinopecten yessoensis antiviral ability is enhanced. Moreover the survival rate and the livability are high. The offspring heterozygosity is also enhanced through the group fertilization.

The invention relates to a feminization method for ZZ zygocyte of Oria tilapia of which the male parent is ZZ and the female parent is WZ, belonging to the field of tilapia cultivation. The technical proposal is as follows: before the gonad of the Oria tilapia starts differentiation, feeding the Oria tilapia with tonic that contains estrogen hormone for more than 15 days, and then using the ordinary way of feeding and management to get ZZ type physiologically female fish, wherein every gramme of tonic contains 100 to 200 microgrammes of estrogen hormone, and the temperature for feeding with the tonic is 25 to 30 DEG C. The invention can change the hereditarily male fish into physiologically female fish, such that it sets up a novel breeding system of ZZ type all male Oria tilapia having significant meaning in the expanding and breeding of all male Oria tilapia.

A method for artificial oxytocin and fertilization of Mytilus coruscus comprises the following steps of a parent cultivating pool: building an outdoor cultivating cement pool with the dimension of 10cmx1.5mx1m, stocking inflatable stones, covering canvas above the cement pool to prevent rain and sunlight, and shading the periphery of the cement pool by dark cloth to regulate strength of light; selecting Mytilus coruscus which has new and integral shell surfaces, full gonads, complete gill, light red ovaries and white testes, and is free of damage and powerful in closure of double shells; preparing baits, wherein chlamydomonas live baits including cultured Dunaliella viridis, latymonas subcordiformis and Chaetoceros serve as primary baits, and artificial compound feed including spirulina powder, chlorella powder and yeast tablets serves as secondary baits; drying parent Mytilus coruscus with developed gonads in the shade for 6-8 hours, stimulating the parent parent Mytilus coruscus for 1 hour through running water, and feeding the parent parent Mytilus coruscus into prepared warming seawater.

The invention discloses a powdery feed for promoting gonad development of fugu obscurus. The powdery feed comprises powdery bait, and is characterized in that Chinese herbal medicines and a vitamin complex mixture based on a ratio A and a ratio B, wherein, the Chinese herbal medicines and the vitamin complex mixture are also powdery. When the powdery feed prepared from the Chinese herbal medicines and the vitamin complex mixture is used for feeding the fugu obscurus, irritation to the fish is small, and meanwhile stable gonad development can be promoted so as to realize sexual maturity one sixth of time in advance relative to a natural state, thus achieving higher gonad development degree and improving laid egg (ejaculate) amount and fertilization rate in the case of artificial propagation.

The invention relates to a directional mating and breeding method in Eriocheir sinensis breeding, comprising the following steps of: referring to the Eriocheir sinensis standard GB/T19783-2005, selecting male and female Eriocheir sinensis individuals with mature gonads from different species groups in the first ten-day period of November as parents, after labeling, breeding the male and female Eriocheir sinensis individuals separately, and strengthening cultivation; in the last ten-day period of November, pairing and putting the parents subjected to cultivation strengthening into cylindrical net cages weaved by polyethylene soft plastic, wherein each cylindrical net cage is 50 cm in diameter and 60 cm in height, and the salinity of water is 0.209; distributing a male crab for each net cage, wherein the ratio of female to male of full sib directional mating is 1:1, and the ratio of female to male of half-sib directional mating is 2: 1 or 3: 1; putting the obtained egg-carrying crabs into a 2*667 m<2>*1.5 m outdoor soil pond for overwintering cultivation; in the middle ten-day period of April of next year, pumping out the water in the pond to catch the egg-carrying crabs, putting the egg-carrying crabs into cylindrical net cages weaved by polyethylene soft plastic, wherein the cylindrical net cages are respectively 20 cm in diameter and 50 cm in height and are respectively arranged in net boxes in size of 3*2*1.5 m in other soil ponds respectively, and one egg-carrying crab is arranged in each net box; after the culturing of young crabs is finished, taking out the parents, and performing soil pond cultivation for the juvenile crabs according to the Eriocheir sinensis artificial breeding standard SC/T1099-2007.

The invention discloses a method for inducing Stichopus Japonicus Selenka to lay eggs in vivo, which is characterized by simple operation, short time, synchronous egg laying, large discharge quantity and good quality of eggs, and capability of greatly improving induction rate, fertility rate and hatching rate; the method is implemented as the following steps: dissolving NGLWY acid amides in ultrapure water to formulate 1 mumol/L mother liquor, then dissolving the mother liquor in 0.5mol/L KCl sea water solution to prepare 1nmol/L inducer; selecting gonad mature parent Stichopus Japonicus Selenka with the weight being at least 200g; injecting the inducer with the ratio of volume and the weight of parent Stichopus Japonicus Selenka being 0.1% ml/g but less than 0.3ml into coelom from the back of the Stichopus Japonicus Selenka to perform in vivo induction; and collecting the eggs.

The invention belongs to the field of aquaculture, particularly relates to a light environment regulating method for promoting the gonad maturation of Atlantic salmons. The rapid and synchronous maturation of the ovaries of female Atlantic salmons and the testes of male Atlantic salmons is realized after the breeding of three to six months through the comprehensive regulation of the color, the period and the intensity of light in the living environment of the female Atlantic salmons and the male Atlantic salmons in a mating season or a non-mating season. Irritability stress caused by the traditional methods of delivery induction injection hormones and the like is reduced, the cultivating survival rate of parent fish is increased, and the synchronous maturation of the gonads is promoted.