Method for comprehensively regulating and controlling gonad mature ovulation of cheilinus undulates

Abstract

The invention relates to a method for comprehensively regulating and controlling gonad mature ovulation of cheilinus undulates, which comprises the following steps: preferably selecting, breeding and feeding parent fishes; regulating and controlling water temperature, illumination and water flow to enable the parent fishes to grow; and enhancing the nutrition to promote gonad mature ovulation. The technical method is used for screening artificial ripening effective temperature, intensity of illumination and water flow velocity factor data, screening varieties and doses of artificial ripening effective nutrition enhancers, screening the time suitable for gametogenesis and fertilization of cheilinus undulates, and acquiring high-quality fertilized eggs of cheilinus undulates. The fertilization rate of the eggs of the cheilinus undulates acquired by the method is 20-86%, and the hatching rate is 30-78%. The technical method for comprehensively regulating and controlling gonad mature ovulation of cheilinus undulates, which is provided by the invention, has the characteristics of advancement, high efficiency, practicality and reliability and comprehensively regulates and controls temperature, illumination, water flow and nutrition enhancement in the breeding process of seedlings of cheilinus undulates. The invention has great application value and wide popularization and application prospects for protecting endangered species.

Description

Technical field: [0001] The invention belongs to the reproductive regulation and spawning technology of the rippling fish, which is a method for promoting the maturation and ovulation of gonads of the chrysalis broodstock in an indoor cement pool by artificially controlling light, water temperature and water flow, and comprehensively utilizing nutrition strengthening measures. Background technique: [0002] Cheilinus undulates (commonly known as Cheilinus undulates) is a large wrasse fish with a weight of up to 209kg. It is mainly distributed in the Indian Ocean and the Pacific Ocean. It is one of the two most valuable marine fishes in the world (Cheilinus undulatus.In: IUCN 2008.2008 IUCN Red List of Threatened Species). In 2004, the World Nature and Natural Resources Conservation Fund (IUCN) listed it as one of the 10 endangered creatures including tigers and Asian elephants in Geneva. In the same year, the Convention on International Trade in Endangered Species of Wild Fa...

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Problems solved by technology

Fertilized eggs can be obtained by natural spawning in sea cages, but there are problems such as the small number of broodstock that spawn naturally, the short spawning period, the complicated spawning conditions, the small number of fertilized eggs obtained, and the difficulty in collecting fertilized eggs. , and affected by typhoons, red tides, tides, ocean currents, and seawater temperature, it cannot be popularized and applied in the process of large-scale seed production; fertilized eggs can also be obtained by using hormone induction methods, but high-quality fertilized eggs can only be obtained by hormone induction. The technology is not stable, and there are also the problems that the broodstock gonads have different response times to hormones, and the fertilization rate and hatching rate of eggs vary greatly among batches, which cannot meet the production needs of batch seedlings

Usually under the conditions of artificial temperature, light and water flow regulation, broodstock gonads mature and ovulate, but oocytes often fail to reach final maturity due to lack of nutrients, or the quality is low, which affects the acquisition of high-quality fertilized eggs

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