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Method for inducing gonad maturity and ovulation of verasper variegates parent fish by implanting slow-release hormone

The technology for broodstock and gonads is applied in the field of broodstock breeding, and can solve the problems of low fertilization rate, low rate of successful ovulation broodstock, large differences in egg quality, etc., and achieves low mortality, good ovulation effect and mature high rate effect

Inactive Publication Date: 2010-05-05
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Effect of LH-RHa cholesterol pellet on gonadalmaturation in spotted halibut.) successfully induced broodstock spawning, but the spawning efficiency of broodstock was not high, and the egg quality of each batch was quite different, so the seed production has not been able to achieve a big breakthrough
In recent years, researchers in my country have used HCG and LHRH combined with intramuscular injection in the back to induce the spawning of star flounder (Dalian Tianzheng Industrial Co., Ltd., Meng Xuesong et al., 2007), but the mortality rate of broodstock reached 20-30%, and successful ovulation The ratio of broodstock is low, the egg quality of each batch varies greatly, and the fertilization rate is low
Zhang Quanqi et al. (Ocean University of China, 2004) established a method of slow-release hormones to induce oviposition in star flounder, mainly by intraperitoneal injection of liquid slow-release hormones, which can promote the development and maturation of parent fish gonads, but did not mention the maturation of brood fish eggs, Parental survival and specific oviposition effects, and the risk factor of intraperitoneal injection is high

Method used

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  • Method for inducing gonad maturity and ovulation of verasper variegates parent fish by implanting slow-release hormone
  • Method for inducing gonad maturity and ovulation of verasper variegates parent fish by implanting slow-release hormone
  • Method for inducing gonad maturity and ovulation of verasper variegates parent fish by implanting slow-release hormone

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Experimental program
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Effect test

Embodiment 1

[0028] This research was carried out at the Laizhou base in 2006-2007, and the specific implementation process is as follows:

[0029] 1. Broodstock selection, intensive cultivation and temperature and light to promote maturity:

[0030] For the experiment, 91 broodstocks (49♀:42♂) of stellar flounder older than 3 years were selected. The average weight of female fish was 2118 grams, and the average weight of male fish was 459 grams. The broodstock has normal body shape and body color, and is healthy and undamaged. Broodstock is placed in the rectangular wiped corner breeding pond (30m 2 , The water depth is 1.5 meters) for cultivation. The cultivation water is sand filtered natural seawater, and the water quality conditions are: DO>6.0mg / L, pH=7.6-8.2, and salinity 29-33.

[0031] The bait for the broodstock is mainly chilled small yellow croaker and mixed shrimp. Feed 2 times a day, the daily feeding amount is 2%-3% of the body weight of the fish. Add lecithin to the bait before...

Embodiment 2

[0037] This research was conducted in Qingdao Zhonghai Aquatic Products Co., Ltd. from 2007 to 2008. The specific implementation process is as follows:

[0038] 1. Broodstock selection, intensive cultivation and temperature and light to promote maturity:

[0039] In the experiment, 135 broodstocks (49♀:42♂) of stellar flounder over 3 years old were used. The average weight of female fish was 2309 grams and the average weight of male fish was 488 grams. The broodstock has normal body shape and body color, and is healthy and undamaged. Broodstock is placed in the rectangular wiped corner breeding pond (25m 2 , Water depth of 1.0 meters) for cultivation. The cultivation water is sand filtered natural seawater, and the water quality conditions are: DO>6.0mg / L, pH=7.8-8.2, and salinity 29-33.

[0040] Broodstock bait, feeding method, daily management and temperature and light control measures are the same as in Example 1.

[0041] Using living tissue sampling method or light table method...

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Abstract

The invention relates to a method for inducing gonad maturity and ovulation of a verasper variegates parent fish by implanting a slow-release hormone, which comprises two aspects of cultivating an optimally selected parent fish and adjusting temperature and light to promote the gonad development of the parent fish, and implanting the slow-release hormone for promoting a metrocyte of the parent fish to reach final maturity and ovulation. In the invention, the slow-release hormone is a salmon gonadotropin-releasing hormone analog (sGnRHa) and is coated with a safe and high-efficiency polymer slow-release transitive system (95 percent of cholesterol and 5 percent of cellulose); a female parent fish with gonad in fourth stage is selected by using a living body sampling method or a light table method; a special implanting gun is used for implanting in back muscles, and the effect time of the slow-release hormone lasts for 14-18 days. The method can enable the metrocyte of the verasper variegates parent fish to achieve final maturity and ovulation, has low duress on parent fish reproduction, high rate of parent fish survival and gonad maturity, high fertility rate being 72 percent and high hatching rate being 56.2 percent, and can acquire a bulk of high-quality oosperms. The invention is a set of complete control technique method, can stably regulate and control the development, the maturity and the ovulation of the gonad of the verasper variegates parent fish, and acquire high-quality oosperms.

Description

Technical field: [0001] The invention belongs to the broodstock breeding technology of the star flounder, and is a method for artificially implanting slow-release hormones to promote the final maturation and ovulation of oocytes of the star flounder broodstock and obtain batches of high-quality fertilized eggs. technical background: [0002] Verasper variegates T.&S. (Verasper variegates T.&S.) is mainly distributed in my country’s Yellow Sea, Bohai Sea, East China Sea, Japan, North Korea and other waters. The production is not much. It is a cold-temperature type large benthic fish, mostly living in coastal waters or In the bay, long-distance migration is not done, and it is an excellent species for resource proliferation and artificial breeding. Star flounder has beautiful appearance, large body, delicate and delicious meat, thick and elastic fin edge, rich in vitamins and trace elements, rich in nutrients, easy to cook, and it is loved by consumers and has great market developme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 柳学周陈超徐永江刘新富孙中之王妍妍
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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