WZ(male)-ZZ(female) type Oreochromis aureus ZZ zygote feminization method

A tilapia feminization technology, applied in fish farming, climate change adaptation, application, etc., can solve problems such as failure to achieve success, and achieve a high conversion rate

Inactive Publication Date: 2009-04-29
广州鹭业水产有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • WZ(male)-ZZ(female) type Oreochromis aureus ZZ zygote feminization method
  • WZ(male)-ZZ(female) type Oreochromis aureus ZZ zygote feminization method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Effect of Estrogen Dosage on Feminization Effect

[0015] 1. Materials and methods

[0016] 1.1 Materials

[0017] The Olia tilapia (o.aurus) in this experiment originated from Israel and was introduced from Taiwan after many years of breeding. Its sex determination is WZ♀-ZZ♂type. It is carried out in the seed conservation field of our company's state-level aquatic breeding field.

[0018] 1.2 Method

[0019] 1.2.1 Translational drugs and methods

[0020] The sex conversion experiment of Oria tilapia was carried out with estrogen (FH). The dose of estrogen used is 80-200 micrograms of estradiol per gram of feed, expressed as FH 80 ~FH 200 express. The experimental fry were treated orally at the newly hatched stage, and the bait was fed 3 to 6 times a day. The FH treatment time is divided into three groups: 30 days, 60 days, and 100 days. For each treatment period, gender checks are performed and conversion rates are calculated. At the same time, a control grou...

Embodiment 2

[0040] Influence of timing of estrogen treatment on feminization effect

[0041] 1. Materials and methods

[0042] 1.1 Materials

[0043] 1.1.1 Experimental fish: The broodstock is the original male fish (ZZ♂) of o.aureus introduced from Taiwan and the physiologically transformed female fish (ZZ△♀) obtained from the above research. The newly hatched ZZ fry were obtained by the two-line matching method, that is, ZZ△♀×ZZ♂. This kind of experimental material would appear as all male fish under normal circumstances. The experiments were carried out at the respective testing grounds of Xiamen Luye and Hainan Luye. The Xiamen test site uses late embryos as the experimental material; the Hainan test site uses fish fry (at the end of the hatching stage of the female fish) as the experimental material.

[0044] 1.1.2 Gender transition facilities

[0045] 1.1.2.1 Gender changer: It is redesigned on the basis of summarizing the experience of the previous hatching barrel type changer....

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Abstract

The invention relates to a feminization method for ZZ zygocyte of Oria tilapia of which the male parent is ZZ and the female parent is WZ, belonging to the field of tilapia cultivation. The technical proposal is as follows: before the gonad of the Oria tilapia starts differentiation, feeding the Oria tilapia with tonic that contains estrogen hormone for more than 15 days, and then using the ordinary way of feeding and management to get ZZ type physiologically female fish, wherein every gramme of tonic contains 100 to 200 microgrammes of estrogen hormone, and the temperature for feeding with the tonic is 25 to 30 DEG C. The invention can change the hereditarily male fish into physiologically female fish, such that it sets up a novel breeding system of ZZ type all male Oria tilapia having significant meaning in the expanding and breeding of all male Oria tilapia.

Description

technical field [0001] The invention relates to a WZ♀-ZZ♂ type tilapia breeding technology, more specifically, it relates to a method for feminizing the ZZ zygote of the WZ♀-ZZ♂ type Aurelia tilapia. Background technique [0002] Tilapia is bred in many countries in the world due to its delicious meat, wide feeding habits, fast reproduction, strong adaptability and disease resistance; after the introduction of tilapia species in the 1960s in mainland my country, it has grown rapidly in the 20s. In the late 1980s, the cultivation of tilapia was greatly developed. At present, the overall annual output of the whole country has exceeded 1.2 million tons, and it has become one of the main freshwater economic fishes. In tilapia breeding technology, it is the characteristic of fast reproduction of tilapia, which has also become an important shortcoming that plagues the development of tilapia farming. The reason is that tilapia has early sexual maturity. Cause the overbreeding of ti...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 杨永铨张海明陈远生
Owner 广州鹭业水产有限公司
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