Light environment regulating method for promoting gonad maturation of Atlantic salmons
A technology with mature development and light environment, applied in fish farming, application, climate change adaptation, etc., can solve problems such as inflammatory response, environmental pollution, and large stimulation, so as to promote synchronous development, reduce stress stress, and improve survival rate effect
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Embodiment 1
[0020] Example 1: Light environment regulation of male Atlantic salmon gonad maturation
[0021] (1) The experimental fish were robust Atlantic salmon with a body weight of (850.97±82.77) g. The experimental light source used white, blue and red integrated packaged LED light source floodlights, which were purchased from Jiangsu Wuxi Huazhaohong Optoelectronics Technology Co., Ltd. (see figure 1 ). Testosterone (T) and estradiol (E 2 ) radioimmunoassay kits were purchased from Beijing North Institute of Biotechnology.
[0022] (2) Using L9 (3 4 ) Orthogonal design, 3 factors of light color, photoperiod and light intensity were set, and 3 levels were set for each factor, forming 9 treatment combinations. For each treatment combination, 60 experimental fish (30 fish with PER marking and 30 fish without marking) were reared, totaling 540 fish. The experiment was carried out from August 2012 to March 2013 for a period of 7 months. The water temperature is kept at (15.7±0.4)°C...
Embodiment 2
[0028] Example 2: Light environment regulation of gonad maturation in female Atlantic salmon
[0029] (1) The experimental fish were robust Atlantic salmon with a body weight of (850.97±82.77) g. The experimental light source used white, blue and red COB integrated package LED light source floodlights, and the lamps were purchased from Jiangsu Wuxi Huazhaohong Optoelectronics Technology Co., Ltd. Female testosterone (T) and estradiol (E 2 ) radioimmunoassay kits were purchased from Beijing North Institute of Biotechnology.
[0030] (2) Using L9 (3 4 ) Orthogonal design, 3 factors of light color, photoperiod and light intensity were set, and 3 levels were set for each factor, forming 9 treatment combinations. For each treatment combination, 60 experimental fish were bred (30 with marking and 30 without marking), totaling 540. The experiment was carried out from August 2012 to March 2013 for a period of 7 months. The water temperature is kept at (15.7±0.4)°C, the salinity i...
Embodiment 3
[0037] The difference from Examples 1 and 2 is that the white light is replaced by a fluorescent lamp or an incandescent lamp, and then the male Atlantic salmon and the female Atlantic salmon are treated according to the photoperiod and light intensity of the above examples. It can be seen that it can promote the gonad maturation of both male and female Atlantic salmon, and the sexual maturity ratio of male Atlantic salmon is 42%; the sexual maturity ratio of female Atlantic salmon is 45%.
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