Gonad cell amplification culture medium of Chinese hamster and uses thereof

An ovarian cell, Chinese hamster technology, applied in tissue culture, animal cells, vertebrate cells, etc., can solve the problems of large differences between product batches, unclear ingredients, containing bacteria, etc.

Inactive Publication Date: 2008-06-11
天津百若克医药生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of the current medium for culturing Chinese hamster ovary cells are: At present, animal serum is widely used as a key component of mammalian cell culture medium, mainly bovine serum
However, the disadvantages of animal serum are: expensive, unclear ingredients, large differences between product batches, containing cell growth inhibitory components, and more seriously, may contain bacteria, viruses, etc., resulting in potential biological contamination

Method used

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  • Gonad cell amplification culture medium of Chinese hamster and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0175] The culture medium is composed of basic medium, trace elements, Transferrin and Insulin insulin substitutes. The composition and ingredients of the basic medium are as follows:

[0176] The composition and parts by weight of amino acids are as follows:

[0177] Composition Mg / L

[0178] Glycine 7.5

[0179] L-alanine 8.9

[0180] L-Arginine Hydrochloride 211

[0181] L-asparagine monohydrate 15.01

[0182] L-Aspartic acid 13.3

[0183] L-cysteine ​​hydrochloride 17.56

[0184] L-cysteine ​​hydrochloride monohydrate 31.29

[0185] L-glutamic acid 7.35

[0186] L-histidine hydrochloride monohydrate 31.48

[0187] L-isoleucine 54.47

[0188] L-leucine 59.05

[0189] L-lysine hydrochloride 91.25

[0190] L-methionine 17.24

[0191] L-phenylalanine 5

[0192] L-proline 34.5

[0193] L-serine 10.5

[0194] L-Threonine 11.9

[0195] L-Tryptophan 2.04

[0196] L-Tyrosine Disodium Dihydrate 7.81

[0197] L-valine 46

[0198] The composition and parts by weight...

Embodiment 2

[0256] The culture medium is composed of basic medium, trace elements, Transferrin and Insulin insulin substitutes. The composition and ingredients of the basic medium are as follows:

[0257] The composition and parts by weight of amino acids are as follows:

[0258] Composition Mg / L

[0259]Glycine 2

[0260] L-alanine 3

[0261] L-Arginine Hydrochloride 50

[0262] L-Asparagine Monohydrate 3.0

[0263] L-Aspartic Acid 5.0

[0264] L-cysteine ​​hydrochloride 3.0

[0265] L-cysteine ​​hydrochloride monohydrate 10.0

[0266] L-glutamic acid 4

[0267] L-histidine hydrochloride monohydrate 10

[0268] L-Isoleucine 20

[0269] L-leucine 15

[0270] L-Lysine Hydrochloride 50

[0271] L-methionine 3

[0272] L-Phenylalanine 1.0

[0273] L-Proline 15

[0274] L-Serine 3.0

[0275] L-Threonine 3.0

[0276] L-Tryptophan 0.5

[0277] L-Tyrosine Disodium Dihydrate 3.0

[0278] L-valine 15

[0279] The composition and parts by weight of the vitamins are as follows:

...

Embodiment 3

[0337] The culture medium is composed of basic medium, trace elements, Transferrin and Insulin insulin substitutes. The composition and ingredients of the basic medium are as follows:

[0338] The composition and parts by weight of amino acids are as follows:

[0339] Composition Mg / L

[0340] Glycine 30

[0341] L-alanine 40

[0342] L-Arginine Hydrochloride 450

[0343] L-asparagine monohydrate 45.0

[0344] L-Aspartic Acid 25.0

[0345] L-cysteine ​​hydrochloride 45.0

[0346] L-cysteine ​​hydrochloride monohydrate 65.0

[0347] L-glutamic acid 35

[0348] L-histidine hydrochloride monohydrate 75

[0349] L-Isoleucine 100

[0350] L-Leucine 150

[0351] L-Lysine HCl 300

[0352] L-methionine 40

[0353] L-phenylalanine 15

[0354] L-proline 85

[0355] L-serine 30

[0356] L-threonine 30

[0357] L-Tryptophan 8

[0358]L-Tyrosine Disodium Dihydrate 25

[0359] L-Valine 100

[0360] The composition and parts by weight of the vitamins are as follows:

[03...

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Abstract

The invention relates to a culture medium and the usage thereof, in particular to the Chinese hamster ovary cell enlarging culture medium and the usage thereof. The invention belongs to the biological product field. The Chinese hamster ovary cell enlarging non-protein culture medium of the invention, wherein comprises minimal medium, and also comprises microelement, Transferrin and Insulin subsistent. A serum-containing routine culture medium can be directly replaced with the serum free culture medium of the Chinese hamster ovary cells, and the Chinese hamster ovary cells are not required to adapt process.

Description

technical field [0001] The invention relates to a culture medium and its application, more specifically to a Chinese hamster ovary cell expansion medium and its application. It belongs to the field of biological products. Background technique [0002] Cell culture media are commonly used substances in the field of biological products and are necessary in the cultivation of bacteria and cells. The disadvantages of the current culture medium for Chinese hamster ovary cells are: at present, animal serum is widely used as a key component of mammalian cell culture medium, mainly bovine serum. However, the disadvantages of animal serum are: expensive, unclear ingredients, large differences between product batches, containing cell growth inhibitory components, and more seriously, may contain bacteria, viruses, etc., leading to potential biological contamination. Contents of the invention [0003] 【Problem to be solved】 [0004] The object of the present invention is to provide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06C12N5/071
Inventor 黄宗堂唐贺文
Owner 天津百若克医药生物技术有限责任公司
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