Artificial induced spawning method of Glyptosternum maculatum Regan

A technology of artificial stimuli for P. black spot, which is applied in fish farming, application, climate change adaptation, etc., and can solve problems such as artificial proliferation of P. black spot

Inactive Publication Date: 2009-09-02
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to propose a method for artificially inducing labor of P. black spot to solve the problem of artificial proliferation of P. black spot

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for artificially inducing labor of the black-spotted catfish, the steps of which are as follows:

[0025] (1) Select mature broodstock

[0026] Select mature black-spotted catfish broodstock, choose the male fish of black-spotted catfish as red, squeeze the abdomen with a little white semen to flow out as the male parent fish; And the protruding broodstock is a female broodstock according to the male-to-female ratio of 2-3:1, and the mantissa is counted and weighed for later use;

[0027] (2) Prepare the type and dosage of oxytocin drugs:

[0028] The dosage of the pharmaceutical composition that is used for female broodstock induced labor is: 2mg dioconone maleate / kg body weight (DOM)+10-15mg luteinizing hormone releasing hormone analogue (LDH 2 ) / kg body weight+3-5mg carp pituitary gland / kg body weight (the above three oxytocin drugs were purchased from Zhejiang Ningbo Sansheng Pharmaceutical Co., Ltd.), to obtain the oxytocin (pharmaceutical composition). ...

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Abstract

The invention relates to an artificial induced spawning method of Glyptosternum maculatum Regan originally produced in Yarlung Zangbo river in Tibet. The invention discloses an artificial induced spawning method of the Glyptosternum maculatum Regan. By adopting the exogenous induced-spawning medicine, the gonads of the naturally matured parent Glyptosternum maculatum Regans are promoted to further develop and breed by using the artificial induced spawning method so as to obtain fertilized eggs. The method comprises the following steps: (1) the mature parent Glyptosternum maculatum Regans selected and are matched according to the number ratio of 2-3:1 of the male parent Glyptosternum maculatum Regan and the female parent Glyptosternum maculatum Regan; (2) the induced-spawning medicine composition is prepared, and the dosage of the induced-spawning medicine composition for the female parent Glyptosternum maculatum Regan is 2mg domperidone maleate/kg (weight) + 10-15mg luteotropin releasing hormone d-ala analog/kg (weight) + 3-5mg carp pituitary/kg (weight); the dosage of the induced-spawning medicine composition for the male parent Glyptosternum maculatum Regan is one seconds of that of the induced-spawning medicine composition for the female parent Glyptosternum maculatum Regan; (3) the induced-spawning medicine is prepared to be pasty according to the formula; and (4) an administration method of injection by two needles is provided. The effect time of the prepared induced-spawning medicine composition for the male parent Glyptosternum maculatum Regan and the female parent Glyptosternum maculatum Regan is 10-12h; and the method can achieve the induced spawning ratio of 85 percent.

Description

technical field [0001] The invention belongs to the technical field of fish reproduction, and in particular relates to a method for artificially inducing labor of Glyptosternum maculatum Regan, which is native to the Yarlung Zangbo River Basin in Tibet. Background technique [0002] Using artificial injection of exogenous hormones to make fish spawn is the main way to obtain fertilized eggs of fish. At present, most fish can obtain fertilized eggs through artificial induced spawning. Due to the special gonad structure and special reproductive habits of a small number of fish, it is difficult to artificially induce labor. Because Siluriformes live in the rapids of mountainous areas, their reproductive habits have not been understood by people. In addition, the male gonads are flaky, the amount of semen is small, and the development of male and female gonads is not synchronized. Induction of labor has always been a problem. [0003] So far, only Huang Jikui et al. have repo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 谢从新樊启学李大鹏张慧娟
Owner HUAZHONG AGRI UNIV
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