Salix saposhnikovii tissue culture method

A tissue culture and shrub willow technology, applied in the field of willow breeding, can solve the problems of not being able to maintain the excellent characteristics of fine varieties, small seeds, etc., and achieve the effects of not being limited by time and external conditions, shortening the growth time, and shorting the seedling cycle.

Active Publication Date: 2013-06-26
JIANGSU ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Shrub willow can also be propagated by sowing seedlings, but its seeds are small and should be harvested and sowed, and the original excellent characteristics of the improved species cannot be maintained.

Method used

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  • Salix saposhnikovii tissue culture method
  • Salix saposhnikovii tissue culture method
  • Salix saposhnikovii tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0030] Basic medium configuration:

[0031] The basic medium is WPM medium, in which the components of the major elements and their corresponding use concentrations are as follows: ammonium nitrate (NH 4 NO 3 )400mg / L, calcium nitrate (Ca(NO 3 ) 2 ·4H 2 O)556mg / L, calcium chloride (CaCl 2 ·2H 2 O) 96mg / L, magnesium sulfate (MgSO 4 ·7H 2 O)370mg / L, potassium dihydrogen phosphate (KH 2 PO 4 )170mg / L, potassium sulfate (K 2 SO 4 )990mg / L;

[0032] The components of trace elements and their corresponding use concentrations are as follows: Manganese sulfate (MnSO 4 .H 2 O) 22.3mg / L, zinc sulfate (ZnSO 4 ·7H 2 O)8.6mg / L, boric acid (H 3 BO 3 )6.2mg / L, sodium molybdate (Na 2 MoO 4 ·2H 2 O) 0.25mg / L, copper sulfate (CuSO 4 ·5H 2 O) 0.025mg / L, copper sulfate (CuSO 4 .5H 2 O) 0.025mg / L, cobalt chloride (CoCl 2 .6 2 )0.025mg / L;

[0033] The composition of the iron salt and its corresponding concentration are as follows: Disodium ethylenediaminetetraacetate (Na 2 ·EDTA) 37.3mg / L, ferrous sulfate ...

example 2

[0045] Basic medium configuration:

[0046] The basic medium is MS medium, in which the major element components and their corresponding use concentrations are as follows: ammonium nitrate (NH 4 NO 3 )1650mg / L, potassium nitrate (KNO 3 )1900mg / L, potassium dihydrogen phosphate (KH 2 PO 4 )170mg / L, magnesium sulfate (MgSO 4 ·7H 2 O)370mg / L, calcium chloride (CaCl 2 ·2H 2 O) 440mg / L;

[0047] The components of trace elements and their corresponding use concentrations are as follows: potassium iodide (KI) 0.83mg / L, manganese sulfate (MnSO 4 ·H 2 O) 22.3mg / L, zinc sulfate (ZnSO 4 ·7H 2 O)8.6mg / L, boric acid (H 3 BO 3 )6.2mg / L, sodium molybdate (Na 2 MoO 4 ·2H 2 O) 0.25mg / L, copper sulfate (CuSO 4 .5H 2 O) 0.025mg / L, cobalt chloride (CoCl 2 .6 2 )0.025mg / L;

[0048] The composition of the iron salt and its corresponding concentration are as follows: Disodium ethylenediaminetetraacetate (Na 2 ·EDTA) 37.3mg / L, ferrous sulfate (FeSO 4 ·7H 2 O) 27.8mg / L;

[0049] The components of the organic i...

example 3

[0060] Basic medium configuration:

[0061] The basic medium is 1 / 2MS medium, in which the major element components and their corresponding use concentrations are as follows: ammonium nitrate (NH 4 NO 3 )825mg / L, potassium nitrate (KNO 3 )950mg / L, potassium dihydrogen phosphate (KH 2 PO 4 )85mg / L, magnesium sulfate (MgSO 4 ·7H 2 O) 185mg / L, calcium chloride (CaCl 2 ·2H 2 O) 220mg / L;

[0062] The components of trace elements and their corresponding use concentrations are as follows: potassium iodide (KI) 0.83mg / L, manganese sulfate (MnSO 4 ·H 2 O) 22.3mg / L, zinc sulfate (ZnSO 4 ·7H 2 O)8.6mg / L, boric acid (H 3 BO 3 )6.2mg / L, sodium molybdate (Na 2 MoO 4 ·2H 2 O) 0.25mg / L, copper sulfate (CuSO 4 .5H 2 O) 0.025mg / L, cobalt chloride (CoCl 2 .6 2 )0.025mg / L;

[0063] The composition of the iron salt and its corresponding concentration are as follows: Disodium ethylenediaminetetraacetate (Na 2 ·EDTA) 37.3mg / L, ferrous sulfate (FeSO 4 ·7H 2 O) 27.8mg / L;

[0064] The components of the organic ...

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Abstract

The invention discloses a salix saposhnikovii tissue culture method. The salix saposhnikovii tissue culture method comprises the following steps of: (1) material selection and disinfection treatment; (2) cultivation of tube seedlings; (3) proliferation and rooting cultivation; and (4) acclimatization of seedlings and transplanting. The salix saposhnikovii tissue culture method provided by the invention is high in propagation efficiency and small in occupied space without being limited by seasons or outside environments, needs few parent materials, and can provide a large number of high-quality nursery stocks in a short term. In addition, the successful establishment of an efficient salix saposhnikovii tissue regeneration system has great significance for the development of work such as biological research, genetic improvement and multi-target molecular breeding of salix saposhnikovii.

Description

Technical field [0001] The invention relates to a method for breeding willows, in particular to a method for tissue culture of shrub willows. Background technique [0002] Willow is a generic term for Salix plants in the Salicaceae family. There are 526 species in the world, mainly distributed in the temperate regions of the northern hemisphere. There are 257 species, 120 varieties and 33 varieties in China, most of which are shrub willow. Shrub willow has a wide distribution, fast growth, high yield, high calorific value, strong stress resistance, easy renewal, and a wide range of wood uses. It is an important tree species for protection forests, water and soil conservation forests, landscape forests and timber forests, and is also an energy tree species that has attracted much attention . In particular, there are very rich genetic variation among shrub willow species and their hybrid families and clones. Therefore, the selection of fast-growing, high-biomass, salt-tolerant, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张敏王保松黄利斌陈智敏窦全琴施士争
Owner JIANGSU ACAD OF FORESTRY
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