Method for freezing semen of Macaca fascicularis

Abstract

The invention discloses a method for freezing the semen of a Macaca fascicularis. In the method, fresh semen activity of a semen sample is required to be more than or equal to 0.7. The freezing method comprises the following operating steps of: 1) freezing diluent prescription and diluent preparation for the semen of the Macaca fascicularis; 2) semen dilution; 3) semen package; 4) semen balance; 5) semen freezing; and 6) frozen semen unfreezing. The method has the following advantages that: by using the technology of the invention, in the processes of freezing and unfreezing the semen of the Macaca fascicularis, the motility rate and the recovery rate of the semen after freezing and unfreezing respectively reach 45 percent and 62 percent, so that the reproductive efficiency of good male Macaca fascicularis can be brought into full play, genetic gene of good Macaca fascicularis can be conserved, and referential experience is provided for the research on semen cryopreservation of other rare or endangered primates at the same time.

Description

technical field The invention belongs to the field of biological technology, in particular to a method for freezing cynomolgus monkey semen. Background technique So far, no report identical or similar to the present invention has been found. Contents of the invention The purpose of the present invention is to provide a method for freezing cynomolgus monkey semen. The technical scheme that the present invention solves the problems of the technologies described above is as follows: A method for freezing the semen of cynomolgus monkeys, the semen sample requires fresh semen motility ≥ 0.7, and the freezing method is carried out according to the following steps: 1. The formulation of the frozen dilution of cynomolgus monkey semen and the preparation of the dilution 1) Freeze diluent formula The frozen diluent consists of glucose 111.0mmol / L, TES 52.3mmol / L, Tris 16.5mmol / L, sucrose 5.8mmol / L, lactose 58.4mmol / L, egg yolk with a volume concentration of 20%, glycerol w...

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Have not found so far identical or similar report with the present invention

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