Application of microzyme with osmotolerance

A technology resistant to hypertonicity and yeast, applied in the field of yeast

Active Publication Date: 2013-01-16
GUANGXI XINTIANDE ENERGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Deposit date December 08, 2010

Method used

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Examples

Experimental program
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Embodiment Construction

[0021] The following examples can enable those skilled in the art to understand the present invention more fully, but do not limit the present invention in any way.

[0022] Example 1:

[0023] The specific process is as follows:

[0024] 1. DES mutagenesis selection

[0025] 1) Take a ring of Saccharomyces cerevisiae CICC31481 on the inclined surface of the test tube on the ultra-clean bench, insert it into a 250mL Erlenmeyer flask with 50mL of wort medium, and culture it at 200rpm at 30°C for about 10h, so that the bacteria are in the early logarithmic growth stage .

[0026] 2) Take 5 mL of the bacterial liquid, centrifuge at 5000 rpm for 10 minutes to collect the bacterial cells, and wash twice with normal saline.

[0027] 3) Dilute to 10 with pH7.0 phosphate buffer 7 individual / mL bacterial suspension.

[0028] 4) Take 32mL of pH7.0 potassium phosphate buffer, 8mL of bacterial suspension, and 0.4mL of DES in a 150mL Erlenmeyer flask pre-placed in the rotor and mix th...

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PUM

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Abstract

The invention discloses a microzyme with osmotolerance, belongs to the field of microzymes and particularly relates to a saccharomyces cerevisiae strain with the osmotolerance. As for saccharomyces cerevisiae provided by the invention, the strain is collected in China General Microbiological Culture Collection Center, and the collection number is CGMCC No. 4429. Various performance indexes tracked during experiments of a fermentation tank prove that the strain is normal in metabolism, strong in capability of producing ethanol and low in content of heteroacid, so that the saccharomyces cerevisiae strain with the osmotolerance is excellent.

Description

Technical field: [0001] The present invention belongs to the yeast field, which specializes in high -penetration of high -lying winemark strains. Background technique: [0002] In recent years, due to some special functions, the research of extreme microorganisms has increasingly attracted people's attention.Among them, high -lying yeast is particularly prominent due to its widespread application of various industries such as energy, food and medicine.At present, its main application includes the following aspects: (1) Ethanol: ethanol is an important solvent and fuel. Using high -lying yeast can improve the concentration of the substrates and products in the fermentation liquid, which has the advantages of reducing costs;2) Glycerin: When in a high -seepage environment, most types of yeast cells accumulate glycerin in the cell to facilitate the adjustment of the balance of osmotic pressure;The incremental agent, 60 % to 70 % of the sweetness of sucrose, has the advantages of pre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/18C12P7/06C12R1/865
CPCY02E50/17Y02E50/10
Inventor 李政王玉张健飞
Owner GUANGXI XINTIANDE ENERGY
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