Semen collection method of macaque

Abstract

The invention discloses a semen collection method of macaque, and belongs to the technical field of biology. The method specifically comprises the steps of 1, binding the macaque to a macaque chair; 2, wiping and cleaning glans penis of the macaque with warm normal saline; 3, wiping the glans penis and root of penis with a sterilized wiping article; 4, exciting through a vibrating semen collector; 5, collecting semen. The method is applied to artificial fertilization of macaque, frozen storage of semen, creating of transgenosis and gene knockout macaque, and study on reproductive toxicology and reproductive biology; the reproduction efficiency of quality male macaque can be brought into full play; the genetic gene of quality macaque can be stored; more semen can be collected, and the semen quality is increased; the influence of anesthesia on macaque and semen collection can be avoided; the method is simple and quick to operate, mild and free of wound to macaque, and meets the requirement of animal welfare protection.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for semen collection of rhesus monkeys. Background technique [0002] The method of semen collection by electrical stimulation is the most widely used method of semen collection in rhesus monkeys at home and abroad. It is divided into two types: penile electrical stimulation method and rectal probe method. The traditional electrostimulation semen collection method requires anesthesia, which will have a certain impact on semen collection and pose considerable safety risks. In addition, in the process of electric stimulation for semen collection, it is easy to cause injury and pain to the animal's body, so it does not fully meet the requirements of animal welfare. The current vibration semen collection technology has achieved success in humans and marmosets. This technique has the advantages of non-anesthesia, easy operation, high semen collection efficiency, non-invasive, pa...

AI Technical Summary

Problems solved by technology

However, due to the sensitive personality of macaques, strong alertness and strong aggressiveness in the non-anesthesia state, there have been no reports of macaques successfully using non-anesthesia semen collection techniques before.