38 results about "Freezing methods" patented technology

The invention belongs to the technical field of cell cryopreservation, and relates to a cryopreservation method based on ultrasonic ice planting. In the method, the nucleation temperature of ice planting is less than-9 DEG C, and the cooling rate from nucleation to icing is less than 5 DEG C / min. Compared with the prior art, the method has the advantages that the time required for cryopreservation of the cells is remarkably shortened, meanwhile, damage to the cells caused by ice crystals generated in the cooling process is reduced, and the problem that an existing cell cryopreservation cooling method cannot meet the nucleation and freezing uniformity of a cell solution in the cooling process is solved.

The invention discloses a freezing design method for an inclined shaft freezing crossing strong permeable pebble bed, and particularly relates to the technical field of freezing method construction. Acquiring rock and soil parameters and regional historical environment temperature of a pebble bed of a crossing section of the target inclined shaft; establishing a three-dimensional inclined shaft geometric model, and determining the arrangement position, inclination angle and spacing of the freezing pipe along the axis of the inclined shaft; constructing a frozen region space model coupled with the pebble bed; the method comprises the following steps: constructing a freezing-heat transfer-seepage multi-field coupling model according to historical environment temperature and freezing pipe arrangement information, performing numerical simulation on a frozen body forming process, and obtaining an evolution function of a freezing boundary expansion range and a freezing thickness at different times; according to the method, multi-physical field coupling modeling and freezing body evolution accurate control of the freezing process can be achieved, and the method has the advantages of being high in predictability, uniform in freezing effect and the like and is particularly suitable for inclined shaft freezing design and construction under complex geological conditions.

The application provides a freezing and recovery method and application of pluripotent stem cell induced differentiation middle stage cells, and the freezing method comprises the following steps: (1) the starting cells are pluripotent stem cells, which are induced to the middle stage; (2) the cells are recovered and counted by using a mild digestion enzyme, and the cells are resuspended in a freezing solution; (3) after programmed cooling to-80 DEG C, the cells are transferred into liquid nitrogen for storage, and are ready for use. The freezing method and the freezing solution provided by the application significantly improve the freezing survival rate of the differentiation middle stage cells, avoid the apoptosis or irreversible stress damage induced by the traditional freezing solution, and obviously improve the problems of cell damage and low survival rate caused by the general freezing solution in the prior art. The differentiation potential of the recovered cells is good, and the cells can successfully form mature kidney unit structures, so that the problem of subsequent differentiation failure in the prior art is solved.

The invention discloses a magnetic field auxiliary circulating liquid microcrystal freezing system which comprises a refrigerator body, a magnetic field generator is arranged in the refrigerator body, the magnetic field generator is connected with a transformer through an external control port, the magnetic field generator comprises two symmetrically-arranged coils, and a freezing liquid groove is formed between the two coils. A sample tank is placed in the refrigerating fluid tank, refrigerating fluid is contained in the refrigerating fluid tank, the refrigerating fluid tank is connected with an electric self-priming diaphragm pump through a refrigerating fluid circulating pipe and a silica gel hose, and thermocouple temperature measuring equipment is arranged beside the magnetic field generator. According to the invention, the sample is accurately regulated and controlled to enter a supercooled metastable state, and uniform and fine ice crystals can be formed in the food sample in combination with uniform alternating magnetic field auxiliary crystallization generated by the symmetrical coil, so that the mechanical damage of the ice crystals to food cells is greatly reduced.

The invention discloses an electric energy meter data freezing method and an electric energy meter data freezing system for decentralized freezing based on a special data container. The method comprises the following steps of: copying data of all freezing data types required in the special data container from one type of cache to the special data container at the freezing moment; querying a freezing type demand at the current moment, and setting a corresponding bit in the bitmap according to the freezing type demand at the current moment; sequentially querying the bitmap according to bits in a set period, if the bits are set, extracting corresponding frozen data from the special data container according to the frozen data type required by the freezing type corresponding to the bits, writing the corresponding frozen data into the nonvolatile memory, and resetting the bits; after the Nth set period, stopping bitmap query, clearing the bitmap, and waiting for the next freezing moment; n is the number of defined freezing types, and the set period time * N is smaller than the minimum freezing period. According to the method, system jamming caused by writing a large amount of frozen data into the nonvolatile memory at the freezing moment can be avoided.

The invention relates to the technical field of coal mine shaft construction, in particular to an active thermal insulation control method under the freezing method construction condition. Comprising freezing holes formed in the periphery of a shaft, heat insulation holes and temperature measurement holes located between a derrick foundation and the freezing holes, a heat preservation plate pasted outside the derrick foundation, and a temperature, pressure and deformation monitoring system. The method comprises the following steps: starting independent liquid circulation of a freezing hole and a thermal insulation hole, starting a monitoring system, and collecting multi-source real-time data such as temperature, pressure and deformation in real time; inputting the data into a preset data fusion and intelligent prediction control system, and predicting the frost heaving deformation trend of the future derrick foundation through a built-in prediction model; and according to the prediction result, the liquid inlet temperature and flow of the thermal insulation hole are dynamically adjusted. According to the invention, the conversion from passive thermal insulation to active prediction and intelligent regulation is realized, the safety of the derrick foundation can be effectively protected, and the frost heaving hazard is reduced.

The invention discloses a radio frequency assisted freezing system which comprises a radio frequency generation module, a radio frequency processing module, a low-temperature freezing module and an optical fiber temperature measurement module, the radio frequency generation module can provide stable pulse radio frequency signals and comprises a radio frequency generator, a relay pulse generator and an impedance matcher, and the relay pulse generator and the impedance matcher are electrically connected with the radio frequency generator. The radio frequency processing module is used for providing a radio frequency action space for food and comprises a radio frequency processing device, the low-temperature freezing module can provide freezing cooling capacity for the system and comprises a low-temperature refrigerator, the impedance matcher and the radio frequency processing device are arranged in the low-temperature refrigerator, and the optical fiber temperature measuring module is arranged on the outer side of the low-temperature refrigerator; and the optical fiber temperature measurement module is used for accurately monitoring the food temperature. Through the synergistic effect of the radio frequency field and low-temperature freezing, the crystallization process of water molecules in the food can be effectively interfered, the food is promoted to generate an obvious supercooling phenomenon, a large number of ice nucleuses are formed at the temperature lower than the freezing point in an explosive mode finally, and the final quality of the frozen food is greatly improved.

The invention particularly relates to a multi-jacket liquid nitrogen ice plug freezing method using a non-uniform gap structure, and belongs to the technical field of nuclear power plant pipeline freezing, and the method comprises the following steps: step 1, determining the total length L1 of jackets and the number N of the jackets of a pipeline freezing section; 2, calculating the value range of the total gap length L2 of the jacket of the frozen section of the pipeline; and step 3, designing and distributing non-uniform gaps. By designing the non-uniform jacket gaps, energy regulation and control are carried out in the ice plug forming process, so that the initial freezing position is accurately controlled, the complete freezing time is shortened, the pressure bearing capacity of the ice plug is improved, and finally energy conservation and efficiency improvement are achieved.

The invention relates to a freezing medium and a freezing method for universal CAR (chimeric antigen receptor) cells. The cryopreservation liquid comprises a compound electrolyte injection, a human serum albumin injection, a sodium chloride injection, a dextran 40 glucose injection, a glucose injection, dimethyl sulfoxide and trehalose. The cell cryopreservation process is deeply analyzed, the specific cell cryopreservation liquid and the cryopreservation method are designed, all the components of the cryopreservation liquid are synergistic, the cryopreservation liquid can adapt to the specific cooling process, cell damage in the cryopreservation process is effectively reduced, the activity of the cryopreserved cells is effectively kept, the cryopreservation liquid can be directly used for reinfusion of patients, a specific sectional programmed cooling program is adopted, and the cell cryopreservation process is simplified. The cell cryopreservation device can further adapt to different cryopreservation volumes, so that the cryopreservation effect is further improved, the survival rate of cells is effectively guaranteed, and the cryopreserved cells still keep good cell activity.

The present invention relates to the technical field of deep underground space stratum reinforcement, and particularly to a CO2 transcritical freezing system and freezing method. The system comprises: a freezing subsystem comprising a first liquid intake pipe and a first liquid return pipe which are arranged on the ground and are configured to convey low-temperature liquid CO2 to the stratum for stratum freezing construction; a liquefaction subsystem for compressing the volume of normal-temperature gaseous CO2, such that the movement of molecules between the gaseous CO2 is restricted and reduced, and the gaseous CO₂ is converted into low-temperature liquid CO2; and an intake and return pipeline subsystem. CO2 media used in the present invention effectively solves the problems of flammability and explosibility, high risk and difficulty in treatment, environmental pollution, etc., caused by liquid ammonia and brine used in traditional freezing construction. Further, a water-bearing stratum, a weak stratum, etc., are frozen and reinforced by means of compression-converted low-temperature liquid CO2 to form a water-proof stratum and a temporary support, thereby effectively preventing seepage of water and sinking, displacement and collapse of a wall surface and a bottom plate, and improving the stability of a frozen area to be excavated.

The invention belongs to the technical field of wine making, and discloses a concentrated wine making method of liquid fermented wine, which comprises the following steps: according to different concentration requirements, adopting a freezing cabin (chamber) freezing mode, a freezer immersion freezing mode, a freezing container low-position (bottom-position) freezing mode and a liquid inert gas injection bottom-position freezing mode; the environment temperature, the temperature of the freezer or the temperature of the bottom of the freezing container reach the freezing point of the needed alcohol content, meanwhile, the alcohol content is monitored through an alcohol content sensor in combination with a manual monitoring mode, and finally the wine with the needed concentration is obtained. The invention discloses a novel method for concentrating liquor in the field of spirit brewing. In the concentration process, more fruit fragrance, grain fragrance, color and nutritional ingredients of the wine making raw materials can be reserved; according to component retention requirements, the liquor is subjected to liquid stabilization and filtration at different temperatures, and components needing to be retained or filtered out can be accurately controlled; and novel wine products can be directly produced, and the concentrated wine liquid prepared by freezing can also be used as blended wine of distilled wine, so that wine products with richer fragrance and content can be produced.

The invention discloses a freezing method construction three-dimensional stress strain-temperature integrated sensor and a measuring method. The sensor comprises a regular tetrahedron framework formed by six rigid rods, and a temperature measuring unit and a strain measuring unit which are respectively arranged in a rod piece, the periphery of the fiber bragg grating area of the strain measurement unit is coated with a heat insulation material, the periphery of the fiber bragg grating area of the temperature measurement unit is coated with a heat conduction material, and temperature and strain are detected in a distinguished mode by measuring Bragg wavelength changes. The measuring method comprises the steps of sensor arrangement, wavelength signal collection and parameter calculation, and three-dimensional stress, strain and temperature data of the soil body in the freezing construction process can be synchronously obtained with high precision. The problems that a traditional discrete sensor is low in integration level, asynchronous in data, poor in environmental adaptability and the like are solved, the device has the advantages of being compact in structure, rapid in response, high in anti-interference performance and the like, and the safety monitoring efficiency and reliability of freezing method construction are remarkably improved.

The invention relates to the field of building construction, and discloses a hydrological hole stop-grouting plug, which comprises a stop-grouting unit which is telescopically arranged on a freezing pipe in a sleeving manner and is used for sealing a gap between the freezing pipe and a hydrological hole; the adjusting unit is arranged on the grout stopping unit; the grouting stopping unit comprises a top support arranged on the freezing pipe in a sleeving mode, intercepting cloth arranged on the outer side of the top support and an adjusting assembly arranged on the top support, connected with the intercepting cloth and used for controlling the intercepting cloth to be opened. Concrete falling into the hydrological hole can be effectively intercepted, it is ensured that grout is fully solidified in a limited area, the problem that the grout cannot be cemented due to the fact that high-flow-speed underground water disperses the injected concrete grout is solved, and the concrete grout cannot sink to the lower portion of the annular space to block the floral tube due to the gravity or water flow scouring effect; and the risk that concrete grout blocks the hydrological hole floral tube section is reduced.

The anti-freezing control device comprises a freezing condition parameter detector capable of detecting a freezing condition parameter indicating whether ice may be attached to a filter in an intake duct, a pressure difference gauge capable of detecting a pressure difference within the intake duct, which is the difference between the pressure upstream of the filter and the pressure downstream of the filter, and a control device main body capable of indicating one of a heating state in which the filter is heated and a non-heating state in which the filter is not heated, for a filter heating device capable of heating the filter according to the freezing condition parameter and the pressure difference.

The invention discloses energy-saving type minced fillet product quick-freezing equipment, and belongs to the technical field of quick-freezing equipment.The energy-saving type minced fillet product quick-freezing equipment comprises a box body, a feeding port is formed in one side wall of the box body, a discharging port is formed in the other side wall of the box body, a conveying belt is installed on the box body, mesh trays are evenly placed on the conveying belt, and the mesh trays are arranged on the conveying belt; the width of the mesh tray is larger than that of the conveying belt, a liquid nitrogen pipeline is connected to the top wall of the box body, one end of the liquid nitrogen pipeline extends into the box body to be connected with a spraying assembly, an electromagnetic valve is installed on the spraying assembly, an exhaust pipe is connected to the bottom of the side wall of the box body, and a pressure balance valve is installed on the exhaust pipe. Through the mesh tray lifting design, the bottoms of surimi products are completely exposed, and through cooperation with control of intermittent conveying, spraying according to needs and spraying stopping buffering, up-down synchronous cooling is achieved, and the quick-freezing period is shortened; and the liquid nitrogen consumption is reduced, the frost layer generation is reduced, the defrosting energy consumption is obviously reduced, and the dual energy-saving effect is prominent.

The utility model discloses a variable-diameter template for freezing method vertical shaft construction, which relates to the technical field of vertical shaft construction and comprises a plurality of first arc-shaped modules, the first arc-shaped modules are arranged on two sides of a second arc-shaped module, and the second arc-shaped module and the first arc-shaped modules are encircled to form a cylindrical barrel. The hydraulic driving assembly is arranged between the multiple first arc-shaped modules, the variable-diameter adjusting mechanism is arranged between the multiple first arc-shaped modules and between the first arc-shaped modules and the second arc-shaped modules, and the sealing structures are arranged between the multiple first arc-shaped modules and between the first arc-shaped modules, the second arc-shaped modules and the variable-diameter adjusting mechanism; according to the variable-diameter formwork for freezing method vertical shaft construction, sealing of the splicing position of the formwork after diameter changing can be achieved, so that the slurry leakage phenomenon at the splicing position during grouting is avoided, pollution to the construction environment is avoided, and the construction quality of a vertical shaft is ensured.

The present application relates to the technical field of deep underground space stratum reinforcement, and particularly relates to a CO2 transcritical freezing system and freezing method, comprising: a freezing subsystem, comprising a first liquid inlet pipe and a first liquid return pipe arranged above the stratum, used for conveying low-temperature liquid CO2 to the stratum to perform stratum freezing construction; a liquefaction subsystem, used for compressing the volume of normal-temperature gaseous CO2, so that the motion of molecules in the gaseous CO2 is limited and reduced, and the gaseous CO2 is converted into low-temperature liquid CO2; and an inlet and return pipe subsystem. The CO2 medium used in the present application effectively solves the problems of flammability, high risk, difficulty in handling and environmental pollution caused by liquid ammonia and salt water used in traditional freezing construction; and the low-temperature liquid CO2 converted by compression is used to freeze and reinforce the aquifer and soft stratum, to form a water-resisting layer and temporary support, effectively preventing water seepage and the sinking, displacement and collapse of the wall surface and the bottom plate, and improving the stability of the freezing area to be excavated.

The invention relates to the technical field of data processing, discloses a digital resource freezing method and device, equipment and a medium, and is used for solving the problem that a current freezing-related business process cannot comprehensively cover a freezing-related business range in digital resources. The method comprises the following steps of: after a freezing instruction of a freezing service is received, acquiring a residual prepayment resource value corresponding to a target account identifier according to the target account identifier by identifying the target account identifier carried in the freezing instruction, a freezing resource value corresponding to the target account identifier and a freezing service type of the freezing service; determining the priority corresponding to the frozen service based on the frozen service type of the frozen service in the freezing instruction, so as to determine the freezing effective state of the frozen service based on the frozen resource value, the residual prepayment resource value, the priority corresponding to the frozen service and a preset waiting rule; and based on the freezing effective state, freezing processing is carried out on the freezing service, so that the integrity of the digital resource freezing service function is ensured.

The application discloses a vehicle invisible door handle unfreezing method, a vehicle invisible door handle unfreezing device, a vehicle invisible door handle unfreezing system, an electronic device and a storage medium. The method comprises the following steps: receiving a handle protruding instruction; determining whether the handle is retracted in a handle hiding area according to the received handle protruding instruction; if the handle is retracted in the handle hiding area, determining whether there is low-temperature frozen matter between the handle and the handle hiding area; if there is low-temperature frozen matter, the handle is in a frozen state, and an ice breaking step is started; determining whether the ice breaking step is ended; if the ice breaking step is ended, the handle protruding instruction is executed; wherein the ice breaking step comprises the following steps: melting or / and breaking the low-temperature frozen matter between the handle and the handle hiding area and synchronously pushing the handle to protrude out of the handle hiding area. Through the above scheme, the bimetallic strip is deformed by temperature rise, accompanied by the inhibition of the surrounding temperature rise, forming a synchronous effect of mechanical deformation and melting of low-temperature frozen matter.

The invention discloses a freezing pipe structure and an experiment platform for simulating well building through a combined construction method, the freezing pipe structure comprises a liquid supply pipe, a split type freezing pipe, an external thread connector and an internal thread connector, the split type freezing pipe comprises a first freezing pipe located in a soil body and a second freezing pipe located outside the soil body, the external thread connector is installed at the end, close to the surface of a soil body, of the first freezing pipe, the internal thread connector is installed on the second freezing pipe, and the first freezing pipe and the second freezing pipe are detachably connected through the external thread connector and the internal thread connector. A cylinder of the simulation experiment platform is arranged on a supporting plate, a reserved hole is formed in the supporting plate, and a detachable cover plate is arranged on a bottom plate of the cylinder corresponding to the reserved hole. A test scene combining a freezing method and mechanical rock breaking is established for the first time, a technical verification and theoretical basis is provided for actual combination of the freezing method and the mechanical rock breaking in subsequent engineering, breakthrough of collaborative application under a freezing and mechanical rock breaking simulation construction method in the field of coal mine well building is promoted, and a foundation is laid for improvement of the overall technical level of the industry.

The present application relates to a kind of support device for shield liaison passage construction and its installation method, support device at least includes: several support main shafts;Support main shaft defines the plane and the axial vertical intersection of shield tunnel, and the adjacent end of support main shaft is connected by support bar, to build complete support frame.The present application is combined with the sliding platform of drilling device by support, and support is not removed after the installation of frozen hole is completed, support bar and support main shaft constitute wedge area, and by setting the mode of multiple groups of support main shaft, the support force of wedge area is guaranteed, to effectively control the time and economic cost of freezing method construction, avoid unnecessary resource waste caused by excessively conservative support design, avoid time and resource waste under the premise of same guaranteeing liaison passage safe construction, give consideration to safety and economy.

1. The name of the design product: laboratory freeze spray granulator. 2. The use of the design product: equipment for preparing various taste-masking, solubilizing, sustained-release, controlled-release, and targeted-release solid dispersions of pharmaceutical solid preparations by using the melt-freeze method. 3. The design points of the design product: in shape. 4. The picture or photo best indicating the design points: perspective view 1.

The present application belongs to the field of analytical technique, and particularly relates to an online sampling system and method for trace fluoride sample in vacuum system. The online sampling system comprises a vacuum container, an orifice plate, an adjusting valve, a hydrolysis device and a freezing container connected in sequence through pipelines. The input end of the vacuum container is connected with a tail pipe and a gas supply cylinder respectively. The hydrolysis device comprises a plurality of hydrolysis bottles connected in sequence. The present application uses the online sampling hydrolysis method to take trace fluoride sample for hydrolysis under the normal operation state of the system, thereby avoiding the problem of long sampling time of the freezing method and the error caused by inaccurate weighing. In order to meet the instrument test requirements, the dilution multiple of the conventional freezing method is 1000-2000 times of the present method. The present method can take trace sample, thereby avoiding the problems of large dilution multiple and large uncertainty of the freezing method, and does not need to break the vacuum, thus avoiding the loss of sample volatilization, and greatly improving the accuracy of the analysis result.

The utility model relates to the technical field of ceramic processing, and provides a ceramic sheet plane processing tool, which comprises a base, a water tank device, a pipe joint and a connecting hose, and by forming a siphon system, liquid generates siphon suction at the bottom of a ceramic sheet, is firmly adsorbed at the bottom of the ceramic sheet and is effectively prevented from sliding. According to the plane machining tool for the ceramic thin plate, the ceramic thin plate is fixed according to the siphon principle, a product can be firmly attached to the rectangular base, the ceramic thin strips on the left side and the right side of the ceramic thin plate can effectively prevent the product from sliding out in the machining process, and the stability of plane machining of the product is improved; the machining tool is used for grinding the ceramic sheet, a freezing method is replaced, refrigeration equipment is omitted, and energy is saved.

The invention relates to a method for cryopreserving several cell clusters (1, 2) of biological cells, comprising the steps of fractionating the cell clusters into at least two fractions (4) based on at least one property of the cell clusters (e.g., size or permeability to CPA), collecting the fractions in different containers (21), and cryopreserving the at least two fractions, wherein specific pretreatment and / or freezing methods are used for each fraction. The pretreatment methods for the fractions differ, for example, in terms of duration or composition of the medium, and the freezing methods for the fractions differ, for example, in terms of cooling rate. The fractionation and cryopreservation processes are carried out in an automated manner.The invention also relates to a cryopreservation device (100) comprising a fractionation device (10), a container device (20) with at least two different containers (21), and a freezing device (30). The fractionation device can also be designed in the form of a fluidic microsystem.

The invention belongs to the technical field of electrolytic hydrogen production, and particularly relates to an electrolytic hydrogen production system and an electrolytic hydrogen production method for seawater desalination through a coupling freezing method. The electrolytic hydrogen production system comprises an electrolytic hydrogen production module, an electrolytic bath waste heat utilization module and a freezing method seawater desalination module; the electrolytic hydrogen production module comprises a hydrogen production electrolytic tank and a liquid supplementing pipeline; the electrolytic bath waste heat utilization module comprises an evaporator, a working medium pump, a condenser, a steam turbine, a first connecting rod and a second connecting rod. The freezing method seawater desalination module comprises a refrigeration compressor, a refrigeration condenser, an expansion valve, a refrigeration evaporator, a seawater crystallization chamber, an ice storage chamber, an ice melting chamber, an ice conveying pipeline I and an ice conveying pipeline II. According to the whole system, through sequential linkage of all the components, collaborative coupling of seawater freezing, desalted water supply, hydrogen production, electrolysis waste heat recovery, power circulation and refrigeration circulation is achieved, and a continuous and stable desalination and hydrogen production operation mode is formed.

The application provides a wind channel system and a freezing method suitable for an American refrigerator. The wind channel system suitable for the American refrigerator comprises a variable-temperature air supply channel and an evaporator in a freezing chamber. The variable-temperature air supply channel and the evaporator are placed side by side in the rear wall of the freezing chamber. The upper part of the variable-temperature air supply channel is connected with a freezing chamber air supply channel. The freezing chamber air supply channel is provided with a wind volume / pressure concentration area. The wind volume / pressure concentration area adopts a fan and an Archimedes spiral line-shaped volute structure to send air into the variable-temperature air supply channel. The freezing method comprises the steps of adopting the fan and the Archimedes spiral line-shaped volute structure to send air into the variable-temperature air supply channel. According to the application, the depth of the freezing chamber drawer can be increased. Meanwhile, in combination with the fan characteristics, the downward wind volume / pressure concentration area can be efficiently formed, the air supply efficiency of the variable-temperature chamber is improved, and the air supply volume of the freezing / cold storage chamber is not affected.

The invention relates to the technical field of muck washing and spraying wastewater treatment, in particular to a muck washing and spraying wastewater decrement purification system and method based on a freezing method, a freezing treatment device is used for receiving washing and spraying water precipitated and filtered by a precipitation filter cartridge, and the precipitation filter cartridge is used for removing solid particles in washing and spraying liquid; according to the invention, the sewage is treated by a freezing method, so that the sewage with complex components can be directly treated, the complexity of multi-stage treatment in the prior art is avoided, a relatively great resource utilization effect can be achieved, and the generated purified water can be considered to be reused for spraying or other links if the water quality reaches the standard, so that resource utilization is realized; formation of ice crystals is slowed down by disturbing water flow, the effect of guiding the icing direction is achieved, compared with the mode that icing guiding is conducted through local temperature rise, the utilization rate of a cold source is higher, the situation that local temperature rise influences the overall cooling speed is avoided, the device can act on a larger area, and the cooling efficiency is improved. The larger the acting area is, the lower the possibility of the ice crystal entrainment phenomenon is.

The present invention provides a refrigerant-containing composition which has a low GWP and which has the characteristics of cooling capacity and COP equivalent to those of R410A. Specifically, the present invention provides a composition which contains difluoromethane (R32) and pentafluoroethane (R125), the total concentration of R32 and R125 being 99.5 mass% or more, and the content ratio of R32 being 53.1-56.0 mass% and the content ratio of R125 being 44.0-46.9 mass% with respect to the total mass of R32 and R125.

This invention belongs to the field of aquaculture breeding technology, and discloses a method and application for rapid freezing of Procambarus clarkii embryos, comprising the following steps: collecting and pre-treating embryos; vitrification freezing, including: transferring embryos into ES solution, equilibrating at room temperature for 10–15 min to allow initial penetration of the cryoprotectant and slight cell dehydration; transferring embryos into VS solution, precisely equilibrating at room temperature for 40 s, quickly picking 10–20 embryos with a carrier, and aspirating excess VS solution; rapid freezing, including: immediately immersing the carrier vertically into liquid nitrogen within 1 second, achieving a cooling rate of >20,000℃ / min; and quickly inserting the carrier into the freezing rack of the liquid nitrogen tank for long-term preservation. This rapid freezing method requires only about 15 minutes for the entire freezing process, and is highly efficient. The vitrified embryos are free of ice crystals, and the embryo structure remains intact. Long-term preservation is possible, ensuring stable germplasm resources.