The invention discloses a large yellow croaker
semen super-long-time preservation and
liquid nitrogen re-freezing method based on a
dry ice medium, and solves the problems that existing
liquid nitrogen preservation and transportation are limited,
dry ice preservation time is short, and
liquid nitrogen long-term preservation cannot be linked. The method comprises the following steps: firstly, mixing seminal fluid with a
sperm diluent containing specific components and a
cryopreservation solution in proportion, balancing at 4 DEG C for 15 minutes, sub-packaging and embedding into
dry ice for preservation; the cryopreserved seminal fluid can be directly recovered for use or put into liquid
nitrogen for re-freezing for long-term preservation. According to the method provided by the invention, the
sperm motility is still up to 90% or above after the
sperm is preserved in dry ice for 72 hours, and the unfrozen
sperm motility can still be kept at 80% or above after the sperm preserved in the dry ice is subjected to liquid
nitrogen re-freezing preservation. According to the method, the difficult problem of safe and convenient ultra-long distance dry ice transportation of the seminal fluid is successfully solved, seamless connection of dry ice long-distance transportation and liquid
nitrogen permanent preservation is realized for the first time, and a powerful
technical support is provided for large yellow croaker cross-regional
germplasm resource collection, preservation, improved variety breeding and industrial
sustainable development.