99 results about "Freezing methods" patented technology

The invention belongs to the technical field of cell cryopreservation, and relates to a cryopreservation method based on ultrasonic ice planting. In the method, the nucleation temperature of ice planting is less than-9 DEG C, and the cooling rate from nucleation to icing is less than 5 DEG C / min. Compared with the prior art, the method has the advantages that the time required for cryopreservation of the cells is remarkably shortened, meanwhile, damage to the cells caused by ice crystals generated in the cooling process is reduced, and the problem that an existing cell cryopreservation cooling method cannot meet the nucleation and freezing uniformity of a cell solution in the cooling process is solved.

The invention provides an anchor-plate-injection collaborative supporting method for active water sealing. The anchor-plate-injection collaborative supporting method is applied to various laneways penetrating through water-containing stratums, especially to construction of vertical shafts constructed through a freezing method. During freezing method construction, a freezing wall is formed firstly, a section height is excavated, an anchor rod is drilled and constructed after the section height is excavated, and if water exists after excavation, forced drainage needs to be carried out to enable the displacement to be larger than the water inflow so as to ensure that no accumulated water exists on a working face; the steel plates are fixed through anchor rods, so that grouting gaps are formed between the steel plates and the well wall; a gap between the bottom edge of the steel plate and the well side is blocked by a certain height through foam; grout is injected through a grouting pipe on the lower portion of the steel plate, and a primary supporting structure is formed after solidification; secondary and more supporting can be carried out immediately or after primary supporting of multiple section heights is formed, and a permanent supporting structure is formed. A traditional passive waterproof mode is broken through, and active water plugging can be achieved; the method adapts to various construction methods and stratums; the temporary support is essentially different from a traditional anchor-shotcrete net temporary support, and the construction efficiency is higher.

The invention provides a contact channel freezing method micro-reinforcement construction method and a control mechanism thereof.The construction method comprises the steps that S1, freezing pipes are arranged and installed, specifically, the freezing pipes are arranged around a contact channel according to a design scheme, it is ensured that the freezing pipes can uniformly cover a construction area, and the freezing pipes are installed and fixed; s2, a freezing liquid injection and freezing process: injecting a freezing liquid through a freezing pipe to gradually freeze the surrounding soil body to form a freezing wall, and meanwhile, monitoring the thickness and strength of the freezing wall in real time to ensure the freezing effect; s3, micro-reinforcement treatment is conducted, specifically, after the frozen wall is formed, a micro-reinforcement technology is used for conducting precise reinforcement on micro cracks and soft areas in the frozen wall; and S4, freezing wall melting and subsequent construction: after construction of the connection channel is completed, injection of the freezing liquid is stopped step by step, so that the freezing wall is gradually melted, and subsequent construction and ending work are carried out. According to the invention, the accuracy and safety of the construction process are ensured.

The present invention is directed to a method for combining dehydrofreezing and medium to far spectrum infrared radiation to preserve biological material. IR transfers energy and penetrates food products, thereby removing naturally present moisture. The present invention provides a novel method of combining pH treatment, infrared radiation, freezing, and vacuum-sealing for preserving biological material. Biological materials are preserved through either a pH treatment or with heat to remove harmful pathogens, followed by processing using far spectrum infrared radiation which may be powered by either gas or electric means. The processed materials undergo a freezing process which may be done using Individual Quick Freezing or blast freezing methods The frozen processed material is then packaged via vacuum sealing.

The invention provides a cryopreservation and resuscitation method for induced differentiation of pluripotent stem cells to metaphase cells and application, and the cryopreservation method comprises the following steps: (1) the initial cells are pluripotent stem cells, and are induced and differentiated to the metaphase; (2) recovering the cells by using a mild digestive enzyme, counting, and resuspending the cells in a cryopreservation solution; and (3) carrying out programmed cooling to-80 DEG C, and then transferring into liquid nitrogen for preservation for later use. According to the cryopreservation method and the cryopreservation liquid provided by the invention, the cryopreservation survival rate of the differentiated metaphase cells is obviously improved, apoptosis or irreversible stress injury induced by a traditional cryopreservation liquid is avoided, and the problems of large cell injury and low survival rate caused by using a general cryopreservation liquid in the prior art are obviously improved. The resuscitated cells keep good differentiation potential, can smoothly form a mature renal unit structure, and solves the problem of subsequent differentiation failure in the prior art.

The invention discloses a freezing design method for an inclined shaft freezing crossing strong permeable pebble bed, and particularly relates to the technical field of freezing method construction. Acquiring rock and soil parameters and regional historical environment temperature of a pebble bed of a crossing section of the target inclined shaft; establishing a three-dimensional inclined shaft geometric model, and determining the arrangement position, inclination angle and spacing of the freezing pipe along the axis of the inclined shaft; constructing a frozen region space model coupled with the pebble bed; the method comprises the following steps: constructing a freezing-heat transfer-seepage multi-field coupling model according to historical environment temperature and freezing pipe arrangement information, performing numerical simulation on a frozen body forming process, and obtaining an evolution function of a freezing boundary expansion range and a freezing thickness at different times; according to the method, multi-physical field coupling modeling and freezing body evolution accurate control of the freezing process can be achieved, and the method has the advantages of being high in predictability, uniform in freezing effect and the like and is particularly suitable for inclined shaft freezing design and construction under complex geological conditions.

The invention discloses a fluid-solid coupling rock mechanics test device for simulating freezing method construction borehole wall stability, which comprises an experiment box body, a freezing pipe is vertically arranged in the middle in the experiment box body, the interior of the freezing pipe is used as a construction area, and a geotechnical material is filled in an area outside the freezing pipe in the experiment box body to serve as a water-bearing layer. The top of the freezing pipe is connected with the refrigerating system, one side of the experiment box is provided with the tide simulation device, the water supply system is connected with the experiment box and the tide simulation device, and the monitoring system is connected with the experiment box. The device can simulate the tide seepage state and monitor the stability of the well wall in real time, can provide more accurate mechanical parameters for freezing method construction on the basis of the fluid-solid coupling rock mechanics principle, and improves the reliability and efficiency of engineering design.

The application provides a freezing and recovery method and application of pluripotent stem cell induced differentiation middle stage cells, and the freezing method comprises the following steps: (1) the starting cells are pluripotent stem cells, which are induced to the middle stage; (2) the cells are recovered and counted by using a mild digestion enzyme, and the cells are resuspended in a freezing solution; (3) after programmed cooling to-80 DEG C, the cells are transferred into liquid nitrogen for storage, and are ready for use. The freezing method and the freezing solution provided by the application significantly improve the freezing survival rate of the differentiation middle stage cells, avoid the apoptosis or irreversible stress damage induced by the traditional freezing solution, and obviously improve the problems of cell damage and low survival rate caused by the general freezing solution in the prior art. The differentiation potential of the recovered cells is good, and the cells can successfully form mature kidney unit structures, so that the problem of subsequent differentiation failure in the prior art is solved.

The invention discloses a cryopreservation method and application of an NK cell cryopreservation solution, and belongs to the technical field of cell cryopreservation. The NK cell cryopreservation solution comprises DMSO, a PBS buffer solution and a hydroxyethyl starch solution; the volume fraction of the DMSO in the NK cell freezing medium is 2.8 to 3.2 percent; the cryopreservation method of the NK cell cryopreservation liquid comprises the following steps: culturing activated NK cells by using a complete culture medium added with IL-15 and IL-18, centrifuging, cleaning, preparing the NK cell cryopreservation liquid, re-suspending to a preset cell concentration by using the NK cell cryopreservation liquid, cooling, freezing, and preserving in liquid nitrogen. The NK cell cryopreservation liquid used in the invention is a universal NK cell cryopreservation liquid, and the cryopreservation method provided by the invention has a good cryopreservation effect and can be used instantly.

The application discloses a kind of along empty roadway short working face coal seam floor high pressure water freezing outburst prevention control method, belong to coal underground mining floor water inrush prevention technical field, comprising the following steps: S1: determine the process parameters of freezing method, and the high pressure water of coal seam floor is detected;S2: first mining area is arranged;S3: excavate freezing chamber and install freezing equipment;S4: construction extends to aquifer freezing borehole;S5: install freezing pipeline and carry out freezing operation;S6: floor high pressure water is pumped out;S7: hydrologic observation borehole is constructed in along empty roadway floor;S8: first working face is mined and excavates second working face recovery roadway;S9: excavate second working face freezing chamber and construction freezing borehole;S10: freezing equipment completes relocation operation before first working face stop mining;S11: repeat S5 to S10 step realizes mining area working face safe mining.The application has the advantages that: it is favorable to improve the production efficiency of mine, and the safety of production is guaranteed.

The invention belongs to the technical field of nuclear power plant pipeline freezing, and discloses a multi-jacket liquid nitrogen ice plug freezing method for nuclear power plant pipeline maintenance, the configuration parameters of ice plug jackets are determined according to pipeline parameters, and the jackets are arranged in a series connection or parallel connection mode to realize freezing; the total length recommendation value of the jackets is obtained according to the pipe diameter checking standard, after the total length is determined, the number of the jackets and the length of gaps are calculated, the gaps are distributed, and when the parallel ice plug jacket mode is adopted, the length of the jackets of all branches is determined according to the branch pipe diameter checking standard by adding the branches, and the number and the pipe diameter of the branches are configured. The freezing time of the ice plug is shortened, meanwhile, the size of the closed ice plug is increased, the freezing efficiency is improved, and energy conservation and efficiency improvement are achieved.

The invention discloses a magnetic field auxiliary circulating liquid microcrystal freezing system which comprises a refrigerator body, a magnetic field generator is arranged in the refrigerator body, the magnetic field generator is connected with a transformer through an external control port, the magnetic field generator comprises two symmetrically-arranged coils, and a freezing liquid groove is formed between the two coils. A sample tank is placed in the refrigerating fluid tank, refrigerating fluid is contained in the refrigerating fluid tank, the refrigerating fluid tank is connected with an electric self-priming diaphragm pump through a refrigerating fluid circulating pipe and a silica gel hose, and thermocouple temperature measuring equipment is arranged beside the magnetic field generator. According to the invention, the sample is accurately regulated and controlled to enter a supercooled metastable state, and uniform and fine ice crystals can be formed in the food sample in combination with uniform alternating magnetic field auxiliary crystallization generated by the symmetrical coil, so that the mechanical damage of the ice crystals to food cells is greatly reduced.

The invention discloses an electric energy meter data freezing method and an electric energy meter data freezing system for decentralized freezing based on a special data container. The method comprises the following steps of: copying data of all freezing data types required in the special data container from one type of cache to the special data container at the freezing moment; querying a freezing type demand at the current moment, and setting a corresponding bit in the bitmap according to the freezing type demand at the current moment; sequentially querying the bitmap according to bits in a set period, if the bits are set, extracting corresponding frozen data from the special data container according to the frozen data type required by the freezing type corresponding to the bits, writing the corresponding frozen data into the nonvolatile memory, and resetting the bits; after the Nth set period, stopping bitmap query, clearing the bitmap, and waiting for the next freezing moment; n is the number of defined freezing types, and the set period time * N is smaller than the minimum freezing period. According to the method, system jamming caused by writing a large amount of frozen data into the nonvolatile memory at the freezing moment can be avoided.

The utility model discloses a freezing method drilling and pressing test integrated device capable of automatically releasing pressure. The freezing method drilling and pressing test integrated device comprises an orifice pipe, an automatic pressure release valve, a gate valve, a drill rod and a pressing device, the other end of the orifice pipe is connected with the gate valve; the automatic pressure release valve is communicated with the orifice pipe; one end, far away from the orifice pipe, of the gate valve is inserted into the drill rod; the pressing device is connected with the gate valve and used for sealing the gate valve and the drill rod. According to the utility model, the valve is arranged on the gate valve, so that the pressurizing pump and the pressurizing pipe can be connected through the valve for pressurizing; and automatic pressure relief is achieved by arranging an automatic pressure relief valve, and harm caused by too large pressure intensity is prevented. And the drilling rod penetrates through the gate valve and then penetrates into the orifice pipe to achieve drilling, the device only needs one-time installation, drilling and pressing tests are taken into consideration at the same time, the number of installation and construction steps is reduced, and the construction workload is reduced. And the device can quickly complete operation, the construction cost is saved, and smooth and safe construction is ensured.

The invention discloses a construction method for reinforcing a soil body through a tunnel connection channel freezing method, and relates to the technical field of tunnel construction.When the construction method is used, in a preset construction area corresponding to a connection channel, drilling construction operation is conducted on a geologic body corresponding to the connection channel, a freezing pipe is installed, and the freezing pipe communicates with a freezing system; freezing operation is conducted on a preset construction area by communicating a freezing system with a freezing pipe, a frozen soil curtain is obtained, excavation and supporting operation is conducted in the frozen soil curtain according to preset construction data, a connection channel is formed, freezing holes are blocked, thaw collapse grouting is conducted on the frozen soil curtain in the connection channel, and the frozen soil curtain is constructed. According to the method, the preset construction area is frozen in real time in the construction process, so that the construction period can be shortened, meanwhile, due to the fact that the preset construction area is frozen in real time in the construction process, the risks of soil collapse and water gushing can be reduced, and the construction safety is improved when the method is used.

The invention relates to the technical field of coal mine shaft construction, in particular to an active thermal insulation control method under the freezing method construction condition. Comprising freezing holes formed in the periphery of a shaft, heat insulation holes and temperature measurement holes located between a derrick foundation and the freezing holes, a heat preservation plate pasted outside the derrick foundation, and a temperature, pressure and deformation monitoring system. The method comprises the following steps: starting independent liquid circulation of a freezing hole and a thermal insulation hole, starting a monitoring system, and collecting multi-source real-time data such as temperature, pressure and deformation in real time; inputting the data into a preset data fusion and intelligent prediction control system, and predicting the frost heaving deformation trend of the future derrick foundation through a built-in prediction model; and according to the prediction result, the liquid inlet temperature and flow of the thermal insulation hole are dynamically adjusted. According to the invention, the conversion from passive thermal insulation to active prediction and intelligent regulation is realized, the safety of the derrick foundation can be effectively protected, and the frost heaving hazard is reduced.

A refrigerator for tender-freezing and refrigeration of meat, and a meat tender-freezing method. The refrigerator for tender-freezing and refrigeration of meat has a tender-freezing working mode, and comprises: a tender-freezing component (1), comprising a refrigeration storage compartment and a cold conduction plate (13) dividing the refrigeration storage compartment into an air cooling chamber (11) and a storage chamber (12) which is used for hermetically storing meat in the tender-freezing working mode, wherein the air cooling chamber (11) and the storage chamber (12) are located on two opposite sides of the cold conduction plate (13); a refrigeration component used for providing cooling air in the tender-freezing working mode; and an air supply component used for receiving in the tender-freezing working mode the cooling air provided by the refrigeration component, and conveying the cooling air to the air cooling chamber (11) so as to perform convective heat exchange with the cold conduction plate (13), wherein in the tender-freezing working mode, the cold conduction plate (13) is cooled by the cooling air and then exchanges heat with the meat in the storage chamber (12) by means of the air in the closed storage chamber (12), so that the meat is supercooled.

The invention discloses a thaw collapse grouting construction method for a connection channel through a freezing method. The method comprises the following steps that S1, freezing reinforcement is conducted, specifically, freezing reinforcement is conducted within the set range of the peripheral side of the connection channel to be excavated between two tunnels, and a frozen soil body is formed; s2, a connecting channel is excavated, specifically, the connecting channel is excavated to communicate the two tunnels, and a supporting and reinforcing device is constructed on the inner side of the connecting channel; s3, thaw collapse grouting is conducted, specifically, grouting is conducted in sequence from inside to outside in the frozen soil body to form multiple circles of grouting soil bodies connected in sequence, and after the inner circle of grouting soil body is solidified, grouting is conducted on the outer circle of grouting soil body; and each circle of grouting soil body is formed by synchronously and symmetrically grouting two sides from bottom to top in a segmented manner, and the upper section is grouted after the lower section is solidified. According to the freezing method connection channel thaw collapse grouting construction method, the grouting range is convenient to control, grouting material waste is not prone to being caused, stress is balanced, structural deformation is avoided, and grout diffusion uniformity and structural durability are improved.

The present invention discloses a refrigerant and its application, and a food freezing method. The refrigerant comprises, by mass percentage, 16% to 37% of amino acids, 10.5% to 15% of sodium chloride, and the remainder comprises water. The present invention provides the application of the above-mentioned refrigerant in food freezing. The food freezing method provided by the present invention comprises: preparing the refrigerant described in the above-mentioned technical solution, immersing the food to be frozen in the refrigerant for freezing, and the solid-liquid mass ratio of the food to be frozen to the refrigerant is 1:6 to 1:20. The refrigerant provided by the present invention can significantly reduce the freezing time and freezing energy consumption of food when used for immersion freezing. The amino acids used can not only lower the freezing point, but also improve the quality or water retention properties of meat products; the infiltration of amino acids and sodium chloride into meat products together can not only realize the integrated freezing and pickling of meat products and reduce the processing procedures, but also increase the product yield and improve the nutritional quality.

The present invention discloses a construction method for a connection passage by the freezing method, which comprises the following steps: Step 1: Install the main tunnel segment (1); Step 2: Drill the freezing pipes; Step 3: After the construction of the freezing pipes (2) is completed, stop the freezing method construction and seal the freezing holes. The present invention can improve the construction efficiency and quality of the connection passage of the underground tunnel by the freezing method and improve the appearance quality of the tunnel.

A quick-freezing method for Trachinotus ovatus. The method comprises the following steps: putting Trachinotus ovatus into a liquid nitrogen flash freezer, the cavity temperature of which is A, and whe

The invention provides a freezing method of a high-moisture meat product and 3D printing vegetable protein meat frozen by adopting the freezing method. According to the freezing method provided by the invention, the freezing rate is 0.08-2 DEG C / min, and / or in the freezing process, the time of passing through the maximum ice crystal generation zone is within 30 min; wherein the moisture content in the high-moisture meat product is 40-80%. By adopting the freezing method provided by the invention, the frozen meat contains a large amount of fine ice crystals, the hardness, chewiness and the like of the frozen and unfrozen meat can be effectively improved, so that the mouth feel of the frozen and unfrozen meat is improved, and the freezing method is suitable for meat products with the water content of 40-80%. The taste of the 3D printing vegetable protein meat prepared by the freezing method is also obviously improved.

The present invention discloses a common orifice device and construction method for drilling holes using the MJS construction method and the freezing construction method for a connecting channel. The common orifice device for drilling holes using the MJS construction method and the freezing construction method for a connecting channel includes an MJS construction orifice pipe, the first end of which is inserted into a tunnel segment and fixedly connected to the tunnel segment, and the inner diameter of the MJS construction orifice pipe matches the diameter of the MJS drill rod; a flange is fixedly connected to the second end of the MJS construction orifice pipe, and the flange on the second end of the MJS construction orifice pipe is detachably fixedly connected to a guard plate via bolts, and the freezing construction orifice pipe is fixedly connected to the guard plate. The first end of the freezing construction orifice pipe is coaxially inserted into the MJS construction orifice pipe, and the inner diameter of the freezing construction orifice pipe matches the diameter of the freezing drill rod. By installing the guard plate and the freezing construction orifice pipe on the MJS construction orifice pipe, the MJS construction method drilling hole position is utilized when drilling the freezing hole, which can effectively reduce the number of holes opened in the tunnel segment.

The invention discloses a radio frequency assisted freezing system which comprises a radio frequency generation module, a radio frequency processing module, a low-temperature freezing module and an optical fiber temperature measurement module, the radio frequency generation module can provide stable pulse radio frequency signals and comprises a radio frequency generator, a relay pulse generator and an impedance matcher, and the relay pulse generator and the impedance matcher are electrically connected with the radio frequency generator. The radio frequency processing module is used for providing a radio frequency action space for food and comprises a radio frequency processing device, the low-temperature freezing module can provide freezing cooling capacity for the system and comprises a low-temperature refrigerator, the impedance matcher and the radio frequency processing device are arranged in the low-temperature refrigerator, and the optical fiber temperature measuring module is arranged on the outer side of the low-temperature refrigerator; and the optical fiber temperature measurement module is used for accurately monitoring the food temperature. Through the synergistic effect of the radio frequency field and low-temperature freezing, the crystallization process of water molecules in the food can be effectively interfered, the food is promoted to generate an obvious supercooling phenomenon, a large number of ice nucleuses are formed at the temperature lower than the freezing point in an explosive mode finally, and the final quality of the frozen food is greatly improved.

The utility model relates to the technical field of shield tunnel construction, and discloses a shield underwater butt joint asymmetric olive-shaped freezing curtain reinforcing water stop structure which comprises a reinforcing body formed by freezing double rows of freezing pipes of a head of a preceding shield tunneling machine and a single row of freezing pipes of a head of a following shield tunneling machine in an asymmetric arrangement mode. The following shield tunneling machine stops tunneling when tunneling is within the range of 0.5-0.7 m outside the cutter head of the preceding shield tunneling machine; according to the freezing pipes, two rows of freezing pipes are arranged at the position of an inner machine head of a preceding shield tunneling machine, a single row of freezing pipes are arranged at the position of an inner machine head of a following shield tunneling machine, the ends of the two shield tunneling machines are reinforced through a freezing method to form an asymmetric olive-shaped reinforcing water stop structure, and under the protection of the reinforcing water stop structure, the two shield tunneling machines can conduct cutterhead replacement and underwater disassembly under normal pressure; by means of the reinforcing structure, shield seabed and river bottom underwater bidirectional tunneling safe butt joint can be achieved, the long-distance underwater tunnel construction efficiency is improved, and meanwhile the underwater tunnel construction risk is reduced.

The utility model discloses a device for simulating dynamic seepage in freezing method construction, relates to the field of urban rail transit construction and underground engineering construction, and aims to solve the problem that the dynamic change of underground water seepage cannot be accurately simulated in the conventional freezing method construction technology. The simulation device can accurately reproduce the seepage direction and flow change of underground water, the device is composed of a base, a first organic glass plate, a second organic glass plate, a fixed side plate, a movable side plate and a top plate, the base is fixedly connected with the first organic glass plate and the second organic glass plate, and the fixed side plate is flexibly adjusted through the movable side plate in sliding fit. The top plate is located above the side plates, the sealing performance and stability of the whole structure are ensured, a flow dividing mechanism is installed on one side of the device, the seepage direction and flow can be accurately adjusted according to needs, meanwhile, the device is provided with an extrusion assembly, and stress environments borne by soil bodies under different burial depths are simulated by applying vertical pressure of different degrees.

The invention particularly relates to a multi-jacket liquid nitrogen ice plug freezing method using a non-uniform gap structure, and belongs to the technical field of nuclear power plant pipeline freezing, and the method comprises the following steps: step 1, determining the total length L1 of jackets and the number N of the jackets of a pipeline freezing section; 2, calculating the value range of the total gap length L2 of the jacket of the frozen section of the pipeline; and step 3, designing and distributing non-uniform gaps. By designing the non-uniform jacket gaps, energy regulation and control are carried out in the ice plug forming process, so that the initial freezing position is accurately controlled, the complete freezing time is shortened, the pressure bearing capacity of the ice plug is improved, and finally energy conservation and efficiency improvement are achieved.

The invention relates to a freezing medium and a freezing method for universal CAR (chimeric antigen receptor) cells. The cryopreservation liquid comprises a compound electrolyte injection, a human serum albumin injection, a sodium chloride injection, a dextran 40 glucose injection, a glucose injection, dimethyl sulfoxide and trehalose. The cell cryopreservation process is deeply analyzed, the specific cell cryopreservation liquid and the cryopreservation method are designed, all the components of the cryopreservation liquid are synergistic, the cryopreservation liquid can adapt to the specific cooling process, cell damage in the cryopreservation process is effectively reduced, the activity of the cryopreserved cells is effectively kept, the cryopreservation liquid can be directly used for reinfusion of patients, a specific sectional programmed cooling program is adopted, and the cell cryopreservation process is simplified. The cell cryopreservation device can further adapt to different cryopreservation volumes, so that the cryopreservation effect is further improved, the survival rate of cells is effectively guaranteed, and the cryopreserved cells still keep good cell activity.

The invention discloses a large yellow croaker semen super-long-time preservation and liquid nitrogen re-freezing method based on a dry ice medium, and solves the problems that existing liquid nitrogen preservation and transportation are limited, dry ice preservation time is short, and liquid nitrogen long-term preservation cannot be linked. The method comprises the following steps: firstly, mixing seminal fluid with a sperm diluent containing specific components and a cryopreservation solution in proportion, balancing at 4 DEG C for 15 minutes, sub-packaging and embedding into dry ice for preservation; the cryopreserved seminal fluid can be directly recovered for use or put into liquid nitrogen for re-freezing for long-term preservation. According to the method provided by the invention, the sperm motility is still up to 90% or above after the sperm is preserved in dry ice for 72 hours, and the unfrozen sperm motility can still be kept at 80% or above after the sperm preserved in the dry ice is subjected to liquid nitrogen re-freezing preservation. According to the method, the difficult problem of safe and convenient ultra-long distance dry ice transportation of the seminal fluid is successfully solved, seamless connection of dry ice long-distance transportation and liquid nitrogen permanent preservation is realized for the first time, and a powerful technical support is provided for large yellow croaker cross-regional germplasm resource collection, preservation, improved variety breeding and industrial sustainable development.

The invention belongs to the technical field of quick-freezing warehouses, and particularly relates to an intelligent molecule quick-freezing warehouse and a quick-freezing method.The intelligent molecule quick-freezing warehouse comprises a box body, a plurality of supporting legs are fixedly installed on the wall face of the bottom of the box body, two box doors are rotatably installed on the wall face of the front side of the box body, a liquid nitrogen tank and a liquid nitrogen evaporator are arranged outside the box body, and a diffusion assembly is arranged on the box body; an adjusting assembly is arranged on the box body; according to the device, a driving motor is controlled to work, the output end of the driving motor works, a plurality of adjusting screw rods rotate in the same direction through transmission of a driven gear, a transmission chain and a driving gear, a connecting sliding plate moves up and down in a sliding groove, the height of a supporting plate is adjusted, and through upward movement of the supporting plate, the height of the supporting plate is adjusted; the space, located in the box body, of the diffusion plate is reduced, liquid nitrogen sprayed out of the diffusion plate can be more intensively distributed around objects, the freezing speed of the objects is increased, and meanwhile loss of the liquid nitrogen is reduced; the problem that an existing quick-freezing storage is low in liquid nitrogen utilization rate is solved.