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Freezing method of pig sperms

A pig sperm and cryopreservation technology, applied in the field of frozen pig sperm, can solve the problems of low in vitro fertilization of pig sperm and low integrity of pig sperm DNA

Inactive Publication Date: 2011-08-03
金一
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Problems solved by technology

However, the exploratory research on the use of LDL for sperm cryopreservation is mainly concentrated on cattle, and the integrity of pig sperm DNA after freezing and thawing is usually not high, and the rate of in vitro fertilization (IVF) of pig sperm after freezing and thawing is not high. high

Method used

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  • Freezing method of pig sperms
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  • Freezing method of pig sperms

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Embodiment Construction

[0016] The present invention will be described in detail below in conjunction with specific embodiments.

[0017] 1.1 Extraction of LDL

[0018] Fresh eggs were collected from a group of chicken farms with the same feeding standard and ration composition, and the egg shells were disinfected with 75% alcohol, and the yolk and egg white were separated after manual breaking. Carefully roll the yolk on a clean filter paper to remove the yolk frenulum and a small amount of egg white adhered to the vitelline membrane, then cut the vitelline membrane with a scalpel blade, and collect the yolk in a beaker pretreated with ice. After the fresh yolk is collected, according to. The method introduced by Moussa et al. [1] to extract LDL first dilute the egg yolk 2-3 times with isotonic saline solution (0.17mol / L NaCI, W / V), and keep stirring for 1h, then centrifuge at 10000×g for 45min at 4°C Afterwards, the supernatant was separated from the precipitate. In order to avoid the interferen...

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Abstract

The invention discloses a freezing method of pig sperms. Unexpectedly, the DNA (deoxyribonucleic acid) completeness and the in vitro fertilization (IVF) rate of unfrozen pig sperms can be obviously improved by using 9(w / v)% of low-density albumin for treatment during the freezing process.

Description

technical field [0001] The invention relates to a culture solution system and a use method for increasing the proliferation rate of animal spermatogonial stem cells, which can make the animal spermatogonial stem cells proliferate in a large amount in a short period of time. Background technique [0002] In the freezing process of porcine semen, including the selection of diluent, the treatment technology of sperm freezing and recovery process, etc., the primary goal is to prevent the formation of ice crystal structures that are enough to kill sperm inside the sperm cells, and at the same time improve the sperm viability after freezing. Try to avoid damage to sperm acrosome and plasma membrane during freezing-thawing. Pace et al. purified egg yolk by ultracentrifugation and found that a low-density component in egg yolk has a cryoprotective effect on sperm. The low-density components in yolk are mainly composed of low-density lipoprotein (10w delasity lipoprotein, LDL), whic...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 金一徐妲方南洙尹熙俊王晓明朴光一
Owner 金一
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