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Stem cell freezing medium and freezing method of stem cells

A cryopreservation method and technology of cryopreservation, which are applied in the preservation, application, and animal husbandry of human or animal bodies, and can solve problems such as biological damage, changes in cell thermodynamics, chemical and physical environments, etc.

Active Publication Date: 2011-03-30
SHENZHEN BEIKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The cryopreservation process will significantly change the thermodynamic, chemical and physical environment of cells, with the accompanying risk of biological damage

Method used

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  • Stem cell freezing medium and freezing method of stem cells
  • Stem cell freezing medium and freezing method of stem cells
  • Stem cell freezing medium and freezing method of stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, study the influence of DMSO concentration in the cryopreservation liquid on the cryopreservation effect

[0039] (1) The first stage of experiment

[0040] Purpose

[0041] To study the effect of DMSO concentration in the cryopreservation solution on the effect of cryopreservation.

[0042] experimental method

[0043] Set up three DMSO concentrations of 5%, 8%, and 10%, and combine with complete medium to form a cryopreservation solution. After digesting the cells according to the conventional method, add the cryopreservation solution, place it in a cryopreservation box, and transfer it to a -80°C refrigerator for the next day. Transfer into liquid nitrogen. After one week of recovery, cell viability and cell status were compared.

[0044] Experimental results

[0045] (1) There was no significant difference in cell viability in each group.

[0046] (2) There was no significant difference in the state of cells in each group. The cells are short s...

Embodiment 2

[0048] Embodiment 2, change HES final concentration, evolve into mass ratio final concentration by volume ratio final concentration, and explore Discuss other feasible freezing methods.

[0049] The purpose of the experiment: to change the final concentration of HES from the final concentration of volume ratio to the final concentration of mass ratio, and to explore other feasible cryopreservation methods.

[0050] Experimental method: with embodiment 1

[0051] Experimental results:

[0052] 1. Discuss the cryopreservation effect of the following scheme: AB is human AB serum; MM is basal medium; FBS is fetal bovine serum; HES is hydroxyethyl starch, the following concentration HES is mass final concentration, and the rest are volume final concentration.

[0053] Table 1

[0054]

[0055] One-way analysis of variance of completely random design data showed no statistically significant difference among the groups. Observation on the first day after recovery and cultur...

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Abstract

The invention provides a stem cell freezing medium and a freezing method of stem cells. The freezing medium contains dimethyl sulfoxide and hydroxyethyl starch and has great clinical application value. The invention also provides the freezing method of stem cells.The stem cell freezing medium in the invention is adopted by the method.In the invention,the cells frozen with the stem cell freezing medium has the advantage of high recovery / survival rate.

Description

technical field [0001] The invention relates to a stem cell cryopreservation solution and a stem cell cryopreservation method. Background technique [0002] The cryopreservation process will significantly change the thermodynamic, chemical and physical environment of cells, with the risk of causing biological damage. The change in temperature mainly depends on the boundary conditions of heat conduction caused by cooling or heating methods, and is also affected by the latent heat effect in the process of cell rethawing. To minimize cell damage during freeze-thaw, chemical and temperature manipulations must be further optimized. However, it is necessary to add one or two cryoprotectants before cooling down, and remove them after dissolving. [0003] In order to maximize the survival rate of cells during recovery, the best freezing conditions are to reduce the formation of crystals in cells as much as possible, and reduce the low-temperature damage to cells caused by high-con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 戴国胜张炳强李国喜郑意端胡祥
Owner SHENZHEN BEIKE BIOTECH
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