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A series of molecular markers and their application in molecular markers of wheat ear germination genes

A technology of molecular markers and ear germination, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc. It can solve the problems of large losses and no breakthroughs in wheat ear germination resistance breeding.

Active Publication Date: 2022-07-15
HENAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because of the large losses caused, breeders from all over the world attach great importance to wheat ear germination, and have conducted a lot of research on its identification methods, resistance mechanisms, genetic mechanisms, and molecular markers. However, there has been no breakthrough in wheat ear germination resistance breeding.

Method used

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  • A series of molecular markers and their application in molecular markers of wheat ear germination genes
  • A series of molecular markers and their application in molecular markers of wheat ear germination genes
  • A series of molecular markers and their application in molecular markers of wheat ear germination genes

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Embodiment

[0044]It should be noted that the main technical idea of ​​this application is: through the phenotypic determination of whether the related gene fragments in Jiejiemai T093 have a reducing effect on the germination of wheat kernels after being introduced into the wheat kernels, and further combined with technologies such as SSR markers and QTL analysis. , the specific design provides a series of molecular markers, which can lay the foundation for further breeding of wheat varieties resistant to ear germination. Therefore, in this embodiment, the relevant experimental situation is briefly described as follows.

[0045] (1) Identification of anti-ear germination phenotype

[0046] Each wheat plant is listed and marked at the flowering stage in April, and enters the wax maturity stage about 35 days after flowering. After all the wheat ears have turned green, each part of the material takes 5 mature and consistent main stem ears and wheat ears. After 7 days of outdoor air-drying, ...

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Abstract

The application belongs to the technical field of molecular breeding of wheat, and specifically relates to a series of molecular markers and their application in molecular markers of wheat ear germination genes. There are 4 molecular markers in total, including one SSR marker and three KASP markers, of which the SSR marker is Xgwm383b marker; the three KASP markers are: A009711, A009180, A009716. In cultivar cultivation, the main way to transfer excellent genes from Jiejiemai to wheat is to create a series of wheat / Jiejiemai substitution lines, and to introduce Jiejiemai chromosomes into the wheat background by means of distant hybridization. For this breeding method, the present application uses the actual F 2 Taking the population as an example, a series of molecular markers were designed to detect and track the chromosome situation of Jiejie introduced into the wheat background, and then used to guide the breeding of new wheat varieties with resistance to ear germination, which has good practical value.

Description

technical field [0001] The application belongs to the technical field of molecular breeding of wheat, and specifically relates to a series of molecular markers and their application in molecular markers of wheat ear germination genes. Background technique [0002] Wheat ear germination (Pre-harvest sprouting, PHS) is a phenomenon of germination on the ear caused by rain or humid environment after the physiological maturity of wheat before harvest. The phenomenon of ear germination not only affects the yield of wheat, but also seriously affects the quality of processing, seed storage and sowing in the next year, resulting in different degrees of economic losses. [0003] Wheat spike germination is a worldwide climatic disaster, and spike germination occurs in wheat producing areas all over the world, causing serious losses. In my country, ear germination mainly occurs in the Yangtze River Basin, the southwest wheat area and the northeast spring wheat area. Large-scale ear ge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 贺洁李锁平张大乐苏亚蕊李玉阁高安礼
Owner HENAN UNIVERSITY
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