A series of molecular markers and their application in molecular markers of wheat ear germination genes
A technology of molecular markers and ear germination, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc. It can solve the problems of large losses and no breakthroughs in wheat ear germination resistance breeding.
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[0044]It should be noted that the main technical idea of this application is: through the phenotypic determination of whether the related gene fragments in Jiejiemai T093 have a reducing effect on the germination of wheat kernels after being introduced into the wheat kernels, and further combined with technologies such as SSR markers and QTL analysis. , the specific design provides a series of molecular markers, which can lay the foundation for further breeding of wheat varieties resistant to ear germination. Therefore, in this embodiment, the relevant experimental situation is briefly described as follows.
[0045] (1) Identification of anti-ear germination phenotype
[0046] Each wheat plant is listed and marked at the flowering stage in April, and enters the wax maturity stage about 35 days after flowering. After all the wheat ears have turned green, each part of the material takes 5 mature and consistent main stem ears and wheat ears. After 7 days of outdoor air-drying, ...
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