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Method and device for identifying transgene tomato based on visible and near-infrared transmission technology

A genetically modified and near-infrared technology, applied in measurement devices, material analysis through optical means, instruments, etc., to achieve the effect of improving modeling speed and accuracy

Inactive Publication Date: 2009-02-18
ZHEJIANG UNIV
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Problems solved by technology

Visible light is more sensitive to color. After research, there is no report on the method and device for quickly identifying transgenic tomatoes using visible and near-infrared transmission technology in China.

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  • Method and device for identifying transgene tomato based on visible and near-infrared transmission technology
  • Method and device for identifying transgene tomato based on visible and near-infrared transmission technology
  • Method and device for identifying transgene tomato based on visible and near-infrared transmission technology

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Embodiment Construction

[0025] The present invention establishes a transgenic tomato identification model based on visible and near-infrared transmission technology, and the process is as follows figure 1 shown. The specific method is:

[0026] 1) For the tomato sample used for identification, wipe off the indicated dirt with a clean cloth, and place it in the test environment for 12-24 hours;

[0027] 2) Collect the spectrum of the modeling sample and collect the spectrum of the modeling sample to find out the characteristic absorption peaks used for the identification of transgenic tomatoes; use the stepwise linear regression (SMLR) method to extract the characteristic information of the spectrum to determine the multiple identifications for the identification of transgenic tomatoes Wavelength, establish a discrimination model, and determine the tomato category:

[0028] C=A 1 -A 2 -A 3

[0029] When b≤C≤a, it is transgenic tomato, when Ca is non-transgenic

[0030] where C is the category, ...

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Abstract

The invention discloses a method for identifying the transgenic tomato based on the visible and near infrared transmission technology and a device thereof. A receiving lens in a detection system is fixed in a central hole of an object stage and a test sample is also placed; the receiving optical fiber is connected with the receiving lens and a fiber spectrometer respectively; the fiber spectrometer is connected with a computer through a USB line; a lamp hanger in an illumination system is arranged above the object stage; halogen lamps are respectively arranged on the horizontal circumference of the lamp hanger and at the center of the top part of the lamp hanger; and an adjusting knob is connected with each halogen lamp. The spectra of a modeling sample is collected to find out the characteristic absorption peaks for transgenic tomato identification; the characteristic information of the spectra is extracted through progressive linear regression to determine a plurality of wavelengths used for transgenic tomato identification and establish an identification model: C is equal to A1 minus A2 minus A3; a sample to be identified is collected to find out A1, A2 and A3; and C is calculated in order to determine the type of the sample to be identified and output the results. The invention avoids the tomato size affecting the identification results and has fast modeling speed and high model accuracy.

Description

technical field [0001] The invention relates to a method and a device for identifying transgenic tomato based on visible and near-infrared transmission technology. Background technique [0002] With the rapid development of biotechnology, genetic engineering technology has been widely used in agriculture, medicine, animal breeding and other fields. The delayed ripening tomato Flavr-Savr developed by Calgene Company in the United States in 1994 TM Since being approved for commercialization, the research and application of genetically modified plants, animals and microorganisms has entered a period of rapid development, especially the commercialization process of genetically modified crops has developed rapidly, and the planting area of ​​genetically modified crops has expanded rapidly worldwide. With the continuous development of commercial production of genetically modified crops, some of the genetically modified agricultural products are directly eaten and used by people t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/35G01N21/3563G01N21/359
Inventor 应义斌谢丽娟徐惠荣蒋焕煜
Owner ZHEJIANG UNIV
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