Eggplant anthocyanin synthesis-related gene SmMYB1 as well as recombinant expression vector thereof and application of gene SmMYB1 in purple tomato culture

An expression vector, eggplant flower technology, applied in the field of genetic engineering, can solve the problems of unstable effect, high cost, long cycle, etc., and achieve good market prospects and economic value

Inactive Publication Date: 2014-08-06
CHONGQING UNIV
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Problems solved by technology

The traditional breeding method has a long cycle, high cost and unstable effect

Method used

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  • Eggplant anthocyanin synthesis-related gene SmMYB1 as well as recombinant expression vector thereof and application of gene SmMYB1 in purple tomato culture
  • Eggplant anthocyanin synthesis-related gene SmMYB1 as well as recombinant expression vector thereof and application of gene SmMYB1 in purple tomato culture
  • Eggplant anthocyanin synthesis-related gene SmMYB1 as well as recombinant expression vector thereof and application of gene SmMYB1 in purple tomato culture

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Embodiment Construction

[0026] Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The experimental method that does not indicate specific condition in the preferred embodiment, usually according to conventional conditions, for example described in molecular cloning experimental guideline (second edition, J. Sambrook etc., translated by Huang Peitang etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0027] The tomato variety used in the examples of the present invention is "Solanum lycopersicon Mill.cv.Ailsa Craig", which is reserved for this laboratory. The pGEM-T Easy vector is the product of Promega, the pBI121 vector, Escherichia coli DH5α, Escherichia coli "helper" bacteria (abbreviated as helper) containing the motile plasmid pRK2013 and Agrobacterium tumefaciens LBA4404 are preserved by our laboratory; RNA extraction kit, RNA PCR Kit (AMV) Ver.3.0 Kit, DNA Extraction Kit Universal Genomic D...

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Abstract

The invention discloses an eggplant anthocyanin synthesis-related gene SmMYB1 as well as a recombinant expression vector thereof and an application of the gene SmMYB1 in purple tomato culture. The nucleotide sequence of the eggplant anthocyanin synthesis-related gene SmMYB1 is as shown in SEQ ID No.3; the gene is constructed into a recombinant expression vector which is used for transforming tomato by use of agrobacterium tumefaciens mediation and then a transgenic tomato is obtained; the phenotypes of petals, stigma, anther, fruits, root, stem and leaves of the obtained transgenic tomato are purple, and therefore, the transgenic tomato can be cultured as an ornamental plant; the content of anthocyanin in the fruits of the transgenic tomato is relatively high and the anthocyanin can be used for reducing the risk of suffering from cancers and cardiovascular diseases, and in short, the transgenic tomato has excellent ornamental and edible values.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and relates to an eggplant anthocyanin synthesis-related gene SmMYB1 and a recombinant expression vector containing the gene, and also relates to the application and method of the gene in cultivating purple tomatoes. Background technique [0002] As a widely cultivated crop in the world, tomato has always been a popular vegetable and fruit. Anthocyanins are water-soluble flavonoid pigments widely present in plants. More and more studies have proved that anthocyanins not only play a positive role in the response of plants to adverse environments (ultraviolet radiation, low temperature, drought and pathogen invasion), but also Anthocyanins also have a certain helpful effect on the treatment of a large number of chronic diseases (cardiovascular diseases and tumors) in humans. A large number of data confirm that regular consumption of foods with high anthocyanin content has important val...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/84C12N1/21A01H5/00C12R1/01
Inventor 胡宗利张彦杰陈国平王苏苏刘霞褚桂花
Owner CHONGQING UNIV
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