Method for removing prunasin from crude extract of passiflora edulia Sims fruit peel
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A technology of prunoside and passion fruit, which is applied in the field of extraction of active ingredients in plants, can solve problems such as potential safety hazards, and achieve low cost effects
Active Publication Date: 2019-08-02
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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[0006] It can be seen that if the anthocyanin extract of passion fruit peel containing prunoside is directly applied to food, medicine and other fields, there will be certain potential safety hazards
Method used
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Embodiment 1
[0025] 1) Take 500g of passion fruit peel (20 mesh), add 10L of ethanol solution with pH=2.5 and concentration of 70v / v% to extract at room temperature for 60min, filter, repeat the above extraction operation twice for the filter residue, combine the filtrate, and recover the obtained extract Ethanol, obtains the crude extract of passion fruit peel;
[0026] 2) Put the crude extract of passion fruit peel on a macroporous resin column (D101) for chromatography, first wash the column with 5BV of pure water with pH=2.5 to remove impurities such as sugar and protein; / v% ethanol solution for elution, collect the eluate with obvious red color, and concentrate in vacuo to obtain concentrated anthocyanin solution;
[0027] 3) Pass the anthocyanin concentrate through a Sephadex LH-20 column, elute with a methanol solution with a pH of 2.5 and a concentration of 10v / v%, and use a UV-visible spectrophotometer and TLC Analyze and monitor the elution process, collect the eluate whose abs...
Embodiment 2
[0037] Repeat Example 1, the difference is:
[0038] In step 3), elute with a methanol solution with a pH of 2.5 and a concentration of 20v / v%.
[0039] Finally, 489 mg of the passion fruit peel anthocyanin extract was obtained.
Embodiment 3
[0041] Repeat Example 1, the difference is:
[0042] In step 2), elute with ethanol solution with pH=2.5 and concentration of 30v / v%;
[0043] In step 3), elute with a methanol solution with a pH of 2.5 and a concentration of 20v / v%.
[0044]Finally, 432 mg of the passion fruit peel anthocyanin extract was obtained.
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Abstract
The invention discloses a method for removing prunasin from a crude extract of passiflora edulia Sims fruit peel. The method comprises the following steps: 1) obtaining the crude extract of the passiflora edulia Sims fruit peel; 2) performing column chromatography through macroporous resin on the crude extract of the passiflora edulia Sims fruit peel, and performing elution and concentration to obtain an anthocyanin concentrated solution; 3) enabling the anthocyanin concentrated solution to pass through a sephadex resin column, eluting with a methanol solution with the pH (Potential of hydrogen) of 1 to 3 and the concentration of 10 to 20 v / v%, collecting fraction containing a target substance, concentrating, and drying to obtain an anthocyanin extract of the passiflora edulia Sims fruit peel. Compared with the prior art, the method creatively selects and uses the methanol solution with the pH of 1 to 3 and the concentration of 10 to 20 v / v% as an eluent for elution when the sephadex resin column is passed through; while the anthocyanin content in the anthocyanin extract of the passiflora edulia Sims fruit peel is effectively improved, the prunasin is also well removed, so that theobtained anthocyanin extract of the passiflora edulia Sims fruit peel can be directly applied to the fields of food and medicine.
Description
technical field [0001] The invention relates to a method for extracting effective components in plants, in particular to a method for removing prunoside from the crude extract of passion fruit peel. Background technique [0002] Passion fruit (Passiflora edulia Sims), also known as egg fruit, is a herbaceous vine of Passiflora genus Passiflora. The main production areas in my country are Guangdong, Guangxi, Taiwan, Fujian and other provinces. Passion fruit juice is rich in nutrients and has a strong and unique fragrance. It has the reputation of "king of juice". Passion fruit is currently mainly used to produce juice, and a very small part is eaten as fresh fruit. In the production of a large amount of passion fruit juice, passion fruit peel is discarded as waste, which not only causes a great waste of resources, but also imposes a burden on the environment. [0003] Anthocyanins are a class of compounds widely present in the flowers, fruits, stems, leaves and other organs...
Claims
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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/065C07H1/08
CPCC07H1/08C07H17/065
Inventor 符毓夏潘争红宁德生李连春
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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