Method for improving yield of transgenic tomato containing astaxanthin and content of astaxanthin in transgenic tomato

A technology of transgenic and astaxanthin, applied in the biological field, can solve problems such as no improvement of cultivation measures, and achieve the effects of simple method, high economic benefit and strong operability

Inactive Publication Date: 2014-12-17
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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Problems solved by technology

In the prior art, there is no report on improving the cultivation measures and i

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  • Method for improving yield of transgenic tomato containing astaxanthin and content of astaxanthin in transgenic tomato

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Embodiment 1

[0017] 1. Materials and Methods

[0018] 1.1 Research materials

[0019] Huang Junchao et al. (Huang JC et al., 2013) screened and isolated the ketolase and hydroxylase genes that can catalyze β-carotene and zeaxanthin into astaxanthin from green algae through genetic engineering methods and transferred them into wild-type In the tomato plant (cv.UC82B), the transgenic tomato obtained (has been preserved in the General Microorganism Center of the China Microbiological Culture Collection Management Committee on May 14, 2014, the preservation number is: CGMCC No. 9223, and the classification name is Shrimp Red 1 No. transgenic tomato. The address of the preservation unit is: No. 3, No. 1 Yard, Beichen West Road, Chaoyang District, Beijing; Tel: 010-64807355; Fax: 010-64807288.) Seeds are sown in small pots and transplanted to the ground two months after emergence inside. Under the premise of ensuring that the cultivation measures such as budding, pruning, and pest control are ...

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Abstract

The invention provides a method for improving the yield of transgenic tomato containing astaxanthin and the content of the astaxanthin in the transgenic tomato. The method comprises the steps of seeding, fertilizing, shading, marking, treatment after fruit collection, freeze-drying, content detection and the like. The transgenic tomato containing the astaxanthin is taken as a research material, so that the transgenic tomato grows under full lighting, and 0.02% of tomato catalyst solution is sprayed in flowering season. A method for improving the content of astaxanthin in astaxanthin engineering tomato by spraying 0.02% of tomato catalyst solution and a method for improving the yield of the astaxanthin engineering tomato by using full lighting growth are provided. The method has the advantages of simplicity, high operability and the like, and higher economic benefits can be brought for the high-accumulation technology and industrialization of the astaxanthin of the astaxanthin engineering tomato.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for increasing the yield and content of astaxanthin engineering tomato. Background technique [0002] Astaxanthin (Astaxanthin) is a secondary carotenoid with super antioxidant activity. Astaxanthin is also known as "astaxanthin". A special red carotenoid discovered has the functions of protecting blood vessels and eyes, anti-radiation, anti-aging, preventing senile dementia and cancer, and has a wide range of application values ​​in the fields of medicine, food, cosmetics, and aquatic feed additives. Natural astaxanthin is often synthesized in the body by some marine microorganisms and a small amount of yeast as a secondary metabolite. However, due to the difficulty of extraction technology and the limitations of algae or strains, the production of astaxanthin is generally low and the cost is high. The disadvantage is that the production of natural astaxanthin ...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G7/06
Inventor 王华杨婷严宁胡虹黄俊潮
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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