Method for acclimatizing tissue culture seedlings of transgenic tomatoes

A tissue culture seedling, transgenic technology, applied in the fields of botanical equipment and methods, cultivation, horticulture, etc., to achieve the effect of improving the survival rate

Inactive Publication Date: 2014-01-29
SICHUAN AGRI UNIV
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  • Abstract
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Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings and deficiencies of the existing methods for the hardening of transgenic tissue cultured seedlings, and provide a method for the hardening of transgenic tomato tissue cultured seedlings with a higher survival rate

Method used

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Embodiment Construction

[0008] The cotyledons of "Zhongshu No. 4" tomato sterile seedlings grown on MS medium for 7 days were used as explants, and the genetic transformation was carried out with Agrobacterium EH105 strain containing the target gene, and the transgenic plantlets grew robustly in the rooting medium , height 4-5cm, well-developed root system, you can harden seedlings. A total of 50 transgenic tomato seedlings were tested. When hardening the seedlings, first open the sealing film of the culture bottle, add 0.5-1cm of distilled water to harden the seedlings in a light incubator at 25°C for 2 days, then take out the seedlings, wash away the residue of the root culture medium, and put The seedlings were transplanted into pots of nutrient soil sterilized by high temperature and watered enough, and the configuration of nutrient soil was flower organic soil: vermiculite: perlite 2:1:1. Cover with a plastic film to keep it moist, and cultivate it in a light incubator at 25°C for one week. Duri...

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Abstract

The invention discloses a method for acclimatizing tissue culture seedlings of transgenic tomatoes. The method comprises the following steps that after the transgenic tissue culture seedlings strongly grow in a rooting culture medium, the height of the seedlings ranges from 4cm to 5cm, and the roots of the seedlings are developed, an opening sealing film of a culture bottle can be opened, 0.5cm to 1cm distilled water is added, the seedlings are acclimated for 2 days in an illumination incubator at the temperature of 25 DEG C, the seedlings are then taken out, residues of the culture medium on the roots are cleaned, the seedlings are transplanted to a nutrient soil flowerpot which is disinfected through high temperature and thoroughly watered, and nutrient soil is composed of flower organic soil, vermiculite and perlite according to the ratio of 2:1:1; the seedlings are covered with a plastic film, so that moisture is preserved and the seedlings are cultured for one week in the illumination incubator at the temperature of 25 DEG C and watered for once through MS culture liquid which is diluted by 10 times during the week; when fresh leaves are grown from the seedlings, the plastic film can be removed; in the transplanting process, the seedlings and the culture soil in the flowerpot are moved to a field together, water and fertilizer management is conducted normally, and the survival rate reaches 100%.

Description

1. Technical field [0001] The invention relates to a method for hardening tomato tissue cultured seedlings, in particular to a method for hardening transgenic tomato seedlings. 2. Background technology [0002] Tomato is rich in nutrition, high in yield and wide in adaptability, and is one of the main vegetable crops all over the world. At the same time, tomato is also one of the first higher model plants to conduct gene transformation research, and its research results are often used for reference in other plants. Therefore, research on tomato transgenesis is one of the hot spots in current plant genetic engineering research. With the development of biotechnology, the research on the genetic improvement of tomato varieties by genetic engineering has made important progress. tomato etc. [0003] At present, the majority of transgenic tomato tissue culture seedlings have a low survival rate, which seriously affects the progress of tomato transgenic research. For example, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G9/10
Inventor 李立芹鲁黎明王西瑶曾富春杨翠芹赵勤
Owner SICHUAN AGRI UNIV
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