Application of plant BIAF gene

A technology of gene and gene silencing, applied in the direction of plant gene improvement, application, plant peptides, etc.

Pending Publication Date: 2022-01-11
TEA RES INST CHINESE ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies only focused on the function of abscisic acid-responsive transcription factors in stress resistance in Arabidopsis, but the function of its homologous gene BIAF in regulating tomato fruit firmness has not been reported

Method used

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  • Application of plant BIAF gene
  • Application of plant BIAF gene
  • Application of plant BIAF gene

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Experimental program
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Embodiment Construction

[0017] The present invention is further described below in conjunction with specific embodiment, but the protection scope of the present invention is not limited to this:

[0018] 1. Obtain the full-length sequence of plant BIAF gene:

[0019] Use Primer Premier 6.0 to design full-gene amplification primers, and take the wild-type tomato Ailsa Craig (AC) leaf cDNA planted in the Huajiachi greenhouse, Hangzhou City, Zhejiang Province as a template (the extraction method of cDNA is a conventional technique, for example, refer to CN 104561025 A ), the specific primers BIAF-F and BIAF-R were designed, and the BIAF fragment was amplified by PCR with PrimerSTAR high-fidelity enzyme.

[0020] The primer sequence is: BIAF-F:5'-ATGGGATCCTACATGAACTTCAAGA-3'

[0021] BIAF-R: 5'-CTACCAAGGTCCTGTCAGTGTCCTT-3'

[0022] PCR amplification reaction system: 2xPrimerSTAR buffer 25ul, dNTP Mixture 5ul, PrimerSTAR DNA polymerase 1ul, ddH 2 O 16ul, cDNA 1ul, upstream and downstream primers each 1...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to an application of a tomato BIAF gene to improvement of fruit hardness. The invention discloses an application of a BIAF gene. The BIAF gene is used for regulating and controlling the hardness of fruits (tomatoes). When the BIAF gene is over-expressed, the hardness of the tomato fruits is improved; and when the BIAF gene is silenced, the hardness of the tomato fruits is reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology; in particular, it relates to the application of tomato BIAF gene in improving fruit firmness. Background technique [0002] Tomato is native to South America and is one of the most widely cultivated vegetable crops in the world. my country's tomato planting area and output are among the top in the world. With the increase of tomato yield, the loss of tomato post-harvest shelf life has become an important problem restricting the development of tomato industry. How to improve the quality of tomato fruit and prolong the storage time of tomato is increasingly attracting the attention of tomato sellers and scientific researchers. Tomato fruit firmness is the primary factor affecting the shelf life of tomato fruits. Improving fruit firmness can significantly prolong the shelf life of tomato fruits; prolonging the shelf life of fruits reduces postharvest losses and effectively avoids the loss of economic b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/08A01H6/82
CPCC07K14/415C12N15/8261C12N15/8218
Inventor 刘浩然汪俏梅
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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