74 results about "ALIZARIN RED" patented technology

The invention discloses a visual test device for penetration grouting of broken coal and rock mass and a test method of the visual test device. The device comprises a fully transparent grouting barrel, wherein the lower end of the fully transparent grouting barrel is connected with a base, a sealing pressure head and a hollow loading pressure head are arranged at the upper end, a sealing ring is arranged around the sealing pressure head, and an exhaust pipe and an exhaust valve are arranged at the top; an electric cement mixing station, a controllable high-pressure pneumatic grouting pump anda digital display multi-element information monitoring system are communicated with a grouting conveying hole and a base grouting hole in the base through a high-pressure-resistant grouting pipe and aflange plate; a high-speed video camera is used for observing the diffusion form and the whole process of slurry containing non-radioactive tracers such as alizarin red or carmine in the broken coaland rock mass in real time. The device and the method have the beneficial effects that the device is transparent and visible, reasonable in structure, simple to manufacture, low in cost, safe to operate and reliable to use.

The invention discloses a method for producing a double-staining plastic-sealed specimen of fish bones. The method comprises the following conditions and steps: cleaning internal organs, scales and skin of fish bodies of fishes with hard and soft bones, fixing by virtue of 10% formalin, soaking with distilled water for 1-2 days so as to completely remove formalin, staining skeletons and soft bones blue by virtue of a dye, namely alcian blue, processing by virtue of alcohols of different concentrations, processing muscles of the fish bodies by virtue of trypsase until the muscles are in a transparent state, simultaneously maintaining the blue of the soft bones, staining the hard bones into red by virtue of alizarin red, carrying out re-transparentization by virtue of different concentrations of mixed solutions of 0.5%KOH and glycerin, plasticizing the fish bodies by virtue of polyvinylpyrrolidone, after the plasticization, carrying out air drying on the specimen, and finally sealing the specimen by virtue of epoxy resin. According to the finished specimen, the skeleton specimen including red hard bones and blue soft bones can be directly seen through the resin and the plasticized muscles.

The invention provides a method for the cloning of a pleurotus djamor HP1 laccase gene and the dye decoloration of recombinase. According to the invention, a pair of degenerate primers is designed according to a conserved region of several white-rot fungi laccase gene copper ion binding site amino acids, a laccase cDNA gene core segment is amplified through RT-PCR, and finally a laccase cDNA full-length sequence is amplified by a 3'/5' RACE technology. The pleurotus djamor cDNA is connected with an expression vector pPICZB to construct a recombinant plasmid pPICZB/lac, and the pichiapastoris SMD1168H is converted by an electric conversion method to obtain an engineering strain. The purified laccase is obtained by DEAE-sepharose CL-6B ion exchange column chromatography, polyethylene glycol concentration and SephadexG-75 gel filtration chromatography. The unique degradation ability of the purified laccase against alizarin red can be applied to the wastewater treatment after dye pollution.

The invention provides a fish branchiostegite marking method. The method comprises the following steps of: 1, selecting water with a pH value of 6.5-7.5, the temperature of 5-25 DEG C and the dissolved oxygen volume of 5-9mg/L as a solvent, and dissolving alizarin red into the solvent so as to prepare an alizarin red aqueous solution with the concentration of 50-200mg/L; 2, after air is inflated in the solution for 6 hours by using an inflator pump, putting fishes to be marked and soaking, and in the process of soaking, always carrying out air inflation operation; 3, enabling the depth of a soaking solution to be 20-50cm higher than the heights of the soaked fishes; 4, carrying out soaking for 12-18 hours, and enabling the soaking time to be inversely proportional to the concentration of the solution; and 5, putting marked fishes into a continuous-flow fishpond to culture for 3 months, wherein red spotted marks with the diameter of 0.2-1.0cm occur at fish branchiostegite. According to the method, the problems that an in-vitro marking method, otolith fluorescent marking and temperature marking and the like cause large damages on fishes, and the mark loss ratio is high are avoided.

The invention discloses a making method for a skeleton-dyed specimen plastic package specimen of cartilaginous fish. The making method comprises the following conditions and steps: cleaning internal organs, scales and the skin of a fish body, fixing the fish body with 10 percent of formalin, soaking the fish body with distilled water for 1 to 2 days to make sure that the formalin is completely cleared away, dyeing the skeleton and cartilages into blue with a dye alcian blue, performing treatment with alcohol at different concentrations, then treating muscles of the fish body with trypsin till a transparent state is achieved, retaining the blue color in the cartilages, dyeing hard bones into red with alizarin red, performing transparent treatment again with 0.5-percent KOH and glycerinum mixed solution at different ratios, then plasticizing the fish body with polyvinylpyrrolidone, after the fish body is plasticized, drying the specimen in air, and finally sealing the specimen with epoxy resin. According to the completed specimen, the skeleton specimen in which the hard bones are red and the cartilages are blue can be seen directly through the resin and the plasticized muscles.

The invention discloses a sulfate radical titration method. The sulfate radical titration method comprises steps as follows: a sample containing sulfate radical is taken, a sample solution is prepared, a MgCl2 solution is added, the mass ratio of Mg<2+> to SO4<2-> is 0.27-7.20, 250-500 mg/g of an acetylacetone solution with the concentration being 0.5-14.0 ml is added, 5-15 mL of an acetic acid solution is added after the mixture is uniformly mixed, the pH (potential of hydrogen) of the solution is 2.0-3.7, 5-20 mL of alcohol solvents are added, the mixture is uniformly mixed, an alizarin red indicator is dropwise added, the mixture is titrated with a barium chloride solution until the mixture becomes pink, and the content of sulfate radical is calculated according to the use amount of barium chloride. The method can be well applied to analysis of the content of sulfate radical in salt lake brine. Besides, the method has the advantages of accuracy, convenience, rapidness, simpleness and the like.

The invention discloses application of miR-489-3p to preparation of medicines for diagnosing and treating human osteoporosis. According to the technical scheme, the detection of thighbone tissue samples of elderly patients with osteoporosis shows that miR-489-3p is low in expression in human osteoporosis tissue samples and can be used as a marker gene for diagnosing osteoporosis; an analogue and an antagonist of miR-489-3p are designed and prepared, then the analogue and the antagonist of the miR-489-3p are transfected into mice pre-osteoblasts MC3T3-E1 for carrying out a mineralization-alizarin red s dyeing experiment and an ALP dyeing experiment; technical detection of qPCR and Western blot shows that miR-489-3p can accelerate osteocyte differentiation and also can carry out targeted regulation on non-receptor protein-tyrosine-phosphatase6 (PTPN6) molecules. The application of miR-489-3p to preparation of medicines for diagnosing and treating human osteoporosis shows that miR-489-3p can regulate PTPN6 molecules to be used as target sites of action of medicines for treating skeleton system diseases such as osteoporosis.

The invention discloses a method for quantitatively analyzing the content of alizarin red through a fluorescence quenching method. According to the method, nitrogen-doped graphene quantum dots are adopted as a fluorescent probe; on the basis of a characteristic that the fluorescence intensity of the nitrogen-doped probe is weakened with the increase of the concentration of alizarin red, high selectivity and high sensitivity detection is performed; and the change value of the fluorescence intensity of the nitrogen-doped graphene quantum dot probe is in a great linear relation with the concentration of the alizarin red, and the correlation coefficient of the fluorescence intensity of the nitrogen-doped graphene quantum dot probe and the concentration of the alizarin red is 0.992. The methodof the invention has the advantages of simple operation, high sensitivity, good selectivity, and quick detection, and is suitable for field in situ analysis.

The invention discloses preparation and application of a hollow spherical-segment-shaped mesoporous silica/chloroperoxidase nanoreactor. According to the nanoreactor, tetraethoxysilane serves as a silicon source, P123 serves as a template agent, and a compound of n-decane and 1,3,5-trimethylbenzene serves as an additive; a hydrothermal synthesis method is utilized for preparing hollow spherical-segment-shaped mesoporous silica firstly, then the mesoporous silica serves as a fixed carrier for fixedly carrying chloroperoxidase. The preparation method is simple, reaction conditions are mild, the obtained mesoporous silica is in a hollow spherical segment shape, the specific surface area is large, the mesoporous diameter range is about 14-18 nm, unimolecular fixation of the chloroperoxidase can be realized, and certain turnover space is reserved for the chloroperoxidase. The hollow spherical-segment-shaped mesoporous silica/chloroperoxidase nanoreactor shows good degradation capacity on alizarin red, the degradation rate still can reach 100% after 8 times of use, the high temperature to which the nanoreactor is resistant is increased to about 50 DEG C, and the acid-base resistance is also improved.

The invention relates to a method for marking larimichthys crocea by using alizarin red. The method comprises the steps that 1, an alizarin red is prepared for mother solution preparation, a mother solution is diluted to reach application concentration, then the salinity is regulated with refined salt and the like to reach 1.5% or above, and an artificial seawater dye liquor is obtained; 2, larimichthys crocea fry to be dyed is soaked in the dye liquor for dyeing, the fry is transferred to natural seawater for rinsing after dyeing is completed, and then larimichthys crocea marked with the alizarin red is obtained. By the adoption of the method, the influence of calcium ions on alizarin red dissolution and precipitation are avoided, the effectively concentration and a dip dyeing effect of the alizarin red are improved, and the problem of massive dead due to the fact that suspending precipitates easily block the gills of the larimichthys crocea is solved.

The invention relates to alizarin red pH response color-changing fibers and a preparation method thereof. According to the preparation method, alizarin red with the dry weight accounting for 0.05%-2% of that of polyacrylonitrile is added into spinning liquid taking 58%-60% sodium sulfocyanate (NaSCN) aqueous solution as a solvent and containing 11%-14% of polyacrylonitrile for wet spinning. The prepared fibers are uniform in coloring and excellent in color fastness, and have weaving mechanical properties and obvious pH response color changing performance.

The invention provides a membrane electrode and a preparation method thereof, a sensor by employing a membrane electrode, an electrochemical workstation and application thereof. The membrane electrodecomprises an electrode and a modified membrane on the surface of the electrode, the modified membrane is a mixture of chitosan and alizarin red S; the modified membrane of the chitosan and the alizarin red S is deposited at the surface of the electrode to allow the obtained membrane electrode to have high sensitivity, high sensing effect and have responsiveness to the current change; the preparation method of the membrane electrode is simple, easy to obtain raw materials, low in cost and easy to implement and can be suitable for industrial large-scale production application; and the sensor comprising the membrane electrode is high in detection sensitivity and good in accuracy; the electrochemical workstation comprising the sensor can achieve the qualitative and quantitative detection of aluminum ions, and is high in sensitivity, small in relative standard deviation and high in accuracy.

The invention discloses a tiopronin concentration determination fluorescence sensor and a preparation method thereof. After hydrotalcite is delaminated, a laminate has positive charges, a guest molecule alizarin red-boric acid system has negative charges, and the hydrotalcite laminate and the guest molecule are self-assembled layer by layer through an electrostatic attraction layer to form a super-molecular structure system and then to be prepared into films; a molecular structure of boric acid is changed by adjusting the layer quantity of the thin films, the concentration of boric acid and the pH value of an alizarin red-boric acid solution system in an assembling process; and a fluorescence sensor thin film with high fluorescence intensity and high stability is obtained through optimized treatment. The fluorescence sensor thin film has the advantages that the preparation method is simple, the cost is low, and quantitative detection of tiopronin is realized. The fluorescence sensor provided by the invention can be used for detecting concentration of tiopronin conveniently and rapidly, and is high in sensitivity and low in detection limit; in the detection process, the sensor does not pollute a system to be detected, and has no reagent loss; and regeneration and repeated utilization of the sensor can be realized.

The invention discloses a method for quantitative analysis of sulphate radical. The method for quantitative analysis of sulphate radical comprises the following steps: controlling alcohol content in a titration system to be 5-35% and controlling the pH value to be 2.0-3.7, adding 0.000491-0.003275% of an alizarin red indicator into the titration system, and titrating barium chloride solution to an end point at a speed of 0.5-3.0 drops/s. The method for quantitative analysis of sulphate radical has the advantages that 19-103mg sulphate radical can be accurately analyzed, and thus analysis accuracy is within 0.3%; and the method for quantitative analysis of sulphate radical is simple and easy to grasp, influence of interfering ion is eliminated, and measurement range of the existing sulphate radical constant analytical method is expanded, so that a new method is provided for quantitatively analyzing content of sulphate radicals, especially sulphate radical content in a complex salt system.

The invention provides melamine test paper and a preparation method thereof, and a method for detecting the melamine. The melamine test paper is prepared by the following steps of: preparing alizarin red, methylene blue and andlauryl sodium sulphate into an aqueous solution, wherein the concentration of each substance in the aqueous solution is: 0.0167 mol/L of the alizarin red, 2.10*10<-5> mol/L of the methylene blue and 0.015 mol/L of the andlauryl sodium sulphate; immersing filter paper into the aqueous solution, taking out and drying; and shearing into strips to obtain the melamine test paper. When the melamine test paper is in use, the method for detecting the melamine comprises the steps of: adding EDTA (Ethylene Diamine Tetraacetic Acid) solid powder into the to-be-detected liquid cream which is prepared from milk or by mixing milk powder; evenly agitating and heating and micro boiling for 5 min; statically placing and depositing; immersing the test paper into the liquid cream liquid supernatant; and wetting and then extracting out. The invention has the advantages of simple using method, and high detecting speed.

The invention discloses a TiO2 immobilized enzyme reactor and application thereof. By using dopamine-modified TiO2 as a carrier and glutaraldehyde as a cross-linking agent, chloroperoxidase or horseradish peroxidase is immobilized on a surface of the carrier through a covalent bonding action to prepare the TiO2 immobilized enzyme reactor. A preparation process is simple and low in cost; the obtained enzyme reactor has high-efficient catalytic degradation capability on dyes (crystal violet, crocein orange G, alizarin red and the like) and 2,4-dichlorophenol, the degradation rate can reach nearly 100 percent within extremely short time, and a repeated use effect is good.