5 results about "ALIZARIN RED" patented technology

The invention provides application of linariin-6-O-glucoside in preparation of a product for preventing and treating osteoporosis, and relates to the field of biological medicine. According to the invention, dyeing experiments of alkaline phosphatase, alizarin red and tartaric acid resistant acid phosphatase prove that the sesaminin-6-O-glucoside has affinity for FPPS receptors, has the capability of promoting bone marrow mesenchymal stem cells to form bone cells, is superior to that of bisphosphate, and can inhibit the generation of osteoclasts; microCT (micro computed tomography) detection results show that the sesaminin-6-O-glucoside has the effect of relieving the osteoporosis and can promote the formation of bone trabecula. According to the application disclosed by the invention, it is proved for the first time that the sesaminin-6-O-glucoside has the effect of resisting osteoclast formation, and meanwhile, the sesaminin-6-O-glucoside also has a better osteogenesis effect, so that a basis is provided for developing the sesaminin-6-O-glucoside as a potential medicine for treating osteoporosis.

This invention provides a standardized preparation method and kit for double-stained transparent specimens of fish skeletons. Orthogonal experiments were designed for fish of different skeleton types (cartilaginous / boneless) and body lengths (small 5-7cm, medium 7-15cm, large 15-25cm), using factors such as KOH concentration and treatment time. Staining effect, transparency, and tissue integrity were used as indicators. Statistical analysis was used to establish an optimal parameter matrix database covering fixation, transparentization, and staining steps. During preparation, the database can be queried based on fish species and body length to obtain differentiated treatment parameters, achieving standardized operation. This invention also provides a kit containing staining stock solution, basic reagents, and a parameter query unit. This invention transforms double-stained skeleton preparation from experience-dependent to data-driven, significantly improving repeatability and success rate, and is suitable for scientific research, teaching, and demonstration.

The invention relates to the technical field of biochemical medicine, in particular to a dry chemical qualitative detection method and a kit for detecting seminal plasma fructose. The method comprises the following steps: by adopting a room-temperature competitive color development system constructed by 3-quinoline boronic acid and alizarin red S, mixing a sample with a special diluent, and dropwise adding the mixture into the kit pre-loaded with test paper; and the judgment can be completed by comparing colors with naked eyes. The operation threshold and the cost limitation of equipment such as high performance liquid chromatography are avoided; the reaction is carried out under mild conditions, and strong corrosive reagents such as concentrated hydrochloric acid are not used, so that the operation safety is guaranteed; the sample treatment only needs simple dilution, and tedious pretreatment steps such as deproteinization, heating or derivatization are not needed, so that the detection efficiency is improved; the selected 3-quinoline boric acid, alizarin red S and auxiliary materials are conventional easily available chemical reagents, and the problem of obtaining special enzyme raw materials is solved. The technical effects of simplicity, convenience, quickness, safety, low cost and easiness in popularization are realized.

The invention discloses a preparation method of a co-immobilized laccase-mediator system, which comprises the following steps: mixing and oscillating a laccase solution and a 2-methylimidazole solution, adding a manganese chloride solution, oscillating and uniformly mixing, reacting the obtained mixed solution in a shaking table for a preset time, sequentially adding p-coumaric acid and 2, 4-dihydroxy-2-methylimidazole, reacting for a preset period of time, filtering, washing, and drying to obtain the co-immobilized laccase-mediator system. The preparation method comprises the following steps: adding a laccase-mediator system MnMOF (at) Lac5 (at) M (at) PEG (Polyethylene Glycol) into a reaction kettle, carrying out oscillation reaction on the laccase-mediator system MnMOF (at) Lac5 (at) M (at) PEG and 2, 2-nitrogen-bis (3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt ABTS for 3-5 hours, then adding polyethylene glycol PEG, centrifuging and washing to obtain the co-immobilized laccase-mediator system MnMOF Compared with free enzyme, the PEG modified laccase-mediator co-immobilization system adopted by the invention has the advantages that the enzyme activity is improved by more than two times, and excellent reusability is shown; under the condition of 30 DEG C, the immobilized laccase-mediator system can realize 100% degradation of alizarin red within 15 minutes, and a green and efficient treatment technology is provided for dye degradation; meanwhile, the system has a good application prospect in the fields of sewage treatment, bioremediation and the like.

1. Name of the designed product: Ceramic cup (blue and white with alizarin red color and joyful small stove type of branch and month of rose). 2. Use of the designed product: holding tea. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo best indicating the design points: perspective view.