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Dye and chemical solution stains for medical purposes are mixtures of synthetic or natural dyes or nondye chemicals in solutions used in staining cells and tissues for diagnostic histopathology, cytopathology, or hematology.
A quantitatively optimized method and kit for preparing transparent fish skeleton specimens stained with Alcian Blue and Alizarin Red.
PendingCN122084352AEliminate dependenciesgood repeatabilityPreparing sample for investigationColor/spectral properties measurementsBiotechnologyAlizarin
This invention provides a standardized preparation method and kit for double-stained transparent specimens of fish skeletons. Orthogonal experiments were designed for fish of different skeleton types (cartilaginous / boneless) and body lengths (small 5-7cm, medium 7-15cm, large 15-25cm), using factors such as KOH concentration and treatment time. Staining effect, transparency, and tissue integrity were used as indicators. Statistical analysis was used to establish an optimal parameter matrix database covering fixation, transparentization, and staining steps. During preparation, the database can be queried based on fish species and body length to obtain differentiated treatment parameters, achieving standardized operation. This invention also provides a kit containing staining stock solution, basic reagents, and a parameter query unit. This invention transforms double-stained skeleton preparation from experience-dependent to data-driven, significantly improving repeatability and success rate, and is suitable for scientific research, teaching, and demonstration.
Owner:SHENYANG UNIV