74 results about "ALIZARIN RED" patented technology

The invention discloses a visual test device for penetration grouting of broken coal and rock mass and a test method of the visual test device. The device comprises a fully transparent grouting barrel, wherein the lower end of the fully transparent grouting barrel is connected with a base, a sealing pressure head and a hollow loading pressure head are arranged at the upper end, a sealing ring is arranged around the sealing pressure head, and an exhaust pipe and an exhaust valve are arranged at the top; an electric cement mixing station, a controllable high-pressure pneumatic grouting pump anda digital display multi-element information monitoring system are communicated with a grouting conveying hole and a base grouting hole in the base through a high-pressure-resistant grouting pipe and aflange plate; a high-speed video camera is used for observing the diffusion form and the whole process of slurry containing non-radioactive tracers such as alizarin red or carmine in the broken coaland rock mass in real time. The device and the method have the beneficial effects that the device is transparent and visible, reasonable in structure, simple to manufacture, low in cost, safe to operate and reliable to use.

The invention discloses a method for producing a double-staining plastic-sealed specimen of fish bones. The method comprises the following conditions and steps: cleaning internal organs, scales and skin of fish bodies of fishes with hard and soft bones, fixing by virtue of 10% formalin, soaking with distilled water for 1-2 days so as to completely remove formalin, staining skeletons and soft bones blue by virtue of a dye, namely alcian blue, processing by virtue of alcohols of different concentrations, processing muscles of the fish bodies by virtue of trypsase until the muscles are in a transparent state, simultaneously maintaining the blue of the soft bones, staining the hard bones into red by virtue of alizarin red, carrying out re-transparentization by virtue of different concentrations of mixed solutions of 0.5%KOH and glycerin, plasticizing the fish bodies by virtue of polyvinylpyrrolidone, after the plasticization, carrying out air drying on the specimen, and finally sealing the specimen by virtue of epoxy resin. According to the finished specimen, the skeleton specimen including red hard bones and blue soft bones can be directly seen through the resin and the plasticized muscles.

The invention relates to the technical field of assay determination of anthracene of polycyclic aromatic hydrocarbon, and in particular relates to an electrochemical method for performing quantitative determination on anthracene content in a water system by taking alizarin red as a probe for modifying a mesoporous material. The material synthesis of a functional modified electrode taking the probe for modifying the mesoporous material is simple and convenient; the modified electrode is simple and convenient to manufacture; the material cost is low; the electrode is easy to update, good in reproducibility and non-toxic, and does not cause environment pollution; and the determining method reduces the interference of coexist substances effectively, and is good in selectivity and high in sensitivity.

The invention provides a method for the cloning of a pleurotus djamor HP1 laccase gene and the dye decoloration of recombinase. According to the invention, a pair of degenerate primers is designed according to a conserved region of several white-rot fungi laccase gene copper ion binding site amino acids, a laccase cDNA gene core segment is amplified through RT-PCR, and finally a laccase cDNA full-length sequence is amplified by a 3' / 5' RACE technology. The pleurotus djamor cDNA is connected with an expression vector pPICZB to construct a recombinant plasmid pPICZB / lac, and the pichiapastoris SMD1168H is converted by an electric conversion method to obtain an engineering strain. The purified laccase is obtained by DEAE-sepharose CL-6B ion exchange column chromatography, polyethylene glycol concentration and SephadexG-75 gel filtration chromatography. The unique degradation ability of the purified laccase against alizarin red can be applied to the wastewater treatment after dye pollution.

The invention provides a fish branchiostegite marking method. The method comprises the following steps of: 1, selecting water with a pH value of 6.5-7.5, the temperature of 5-25 DEG C and the dissolved oxygen volume of 5-9mg / L as a solvent, and dissolving alizarin red into the solvent so as to prepare an alizarin red aqueous solution with the concentration of 50-200mg / L; 2, after air is inflated in the solution for 6 hours by using an inflator pump, putting fishes to be marked and soaking, and in the process of soaking, always carrying out air inflation operation; 3, enabling the depth of a soaking solution to be 20-50cm higher than the heights of the soaked fishes; 4, carrying out soaking for 12-18 hours, and enabling the soaking time to be inversely proportional to the concentration of the solution; and 5, putting marked fishes into a continuous-flow fishpond to culture for 3 months, wherein red spotted marks with the diameter of 0.2-1.0cm occur at fish branchiostegite. According to the method, the problems that an in-vitro marking method, otolith fluorescent marking and temperature marking and the like cause large damages on fishes, and the mark loss ratio is high are avoided.

The invention belongs to the technical field of promotion of the mesenchymal stem cell osteogenic differentiation, and discloses application of magnetic nano materials in promotion of the mesenchymal stem cell osteogenic differentiation. The magnetic nano materials are active ingredients and have the function of promoting a mesenchymal stem cell to differentiate into an osteoblast. Toxicity tests prove that nano ruthenium magnetic nano materials nano-modified by ferric oxide, and the nano selenium magnetic nano materials nono-modified by the ferric oxide are almost nontoxic for the mesenchymal stem cell; alizarin red and oil red O dying proves that the magnetic nano materials prepared have the functions of promoting the mesenchymal stem cell osteogenic differentiation, and inhibiting adipogenic differentiation; the prepared magnetic nano materials such as the nano ruthenium or nano selenium nano-modified by the ferric oxide, are nano-coupled with the ferric oxide in excessive amount directly, and no other auxiliary reagent is needed for the preparation process, the product system is simple, and a product can be preserved and used directly.

The invention discloses a method for labeling alizarin red S of the inner shell of Sepiella maindroni, comprising the following steps of: (1) anesthesia: selecting Sepiella maindroni to be labeled and placing the Sepiella maindroni into an anesthetic solution MS-222 with the concentration ranging from 40ppm to 70ppm for 0.5 minute to 4 minutes; and (2) labeling: injecting a staining solution into the inner shell of the Sepiella maindroni treated in the step (1) with an injector. Because the invention performs anesthesia treatment on the Sepiella maindroni by using the anesthetic solution MS-222, the Sepiella maindroni basically has no stress when injected, the operation can be conveniently carried out, and the harm to the Sepiella maindroni can be reduced. In addition, a mode of staining solution injection is adopted, the injection is carried out at an acute angle, thus, the staining solution can be uniformly distributed on the inner shell favourably, and the invention is especially suitable for Sepiella. Moreover, the method does not need temporary culture for removing floating color after the Sepiella maindroni recovers, and the Sepiella maindroni can be released immediately.

The invention discloses a method for dyeing intermuscular bones of adult megalobrama amblycephala. The method comprises the following steps: step 1, fixing an adult megalobrama amblycephala sample with absolute ethyl alcohol; step 2, rehydrating the adult megalobrama amblycephala sample with the absolute ethyl alcohol and distilled water; step 3, immersing the adult megalobrama amblycephala sample with a TBST solution overnight; step 4, immersing the adult megalobrama amblycephala sample with the distilled water and washing to remove the residual TBST solution; step 5, making the adult megalobrama amblycephala sample transparent with a newly-prepared KOH solution; step 6, transferring the sample to the new KOH solution and dropwise adding an alizarin red dyeing solution to dye until the solution becomes deep purple; step 7, transferring the sample into the new KOH solution and washing off the dyeing solution of the adult megalobrama amblycephala sample; and step 8, transferring the adult megalobrama amblycephala sample to glycerin to be dehydrated and preserved. The method is feasible and an operation process is simple; complicated instruments are not needed; the adult megalobrama amblycephala sample with a transparent body and the intermuscular bones which are dyed into the deep purple color, is obtained; and adhering positions and shapes of the intermuscular bones of the adult megalobrama amblycephala can be clearly observed from the sample.

The invention discloses a making method for a skeleton-dyed specimen plastic package specimen of cartilaginous fish. The making method comprises the following conditions and steps: cleaning internal organs, scales and the skin of a fish body, fixing the fish body with 10 percent of formalin, soaking the fish body with distilled water for 1 to 2 days to make sure that the formalin is completely cleared away, dyeing the skeleton and cartilages into blue with a dye alcian blue, performing treatment with alcohol at different concentrations, then treating muscles of the fish body with trypsin till a transparent state is achieved, retaining the blue color in the cartilages, dyeing hard bones into red with alizarin red, performing transparent treatment again with 0.5-percent KOH and glycerinum mixed solution at different ratios, then plasticizing the fish body with polyvinylpyrrolidone, after the fish body is plasticized, drying the specimen in air, and finally sealing the specimen with epoxy resin. According to the completed specimen, the skeleton specimen in which the hard bones are red and the cartilages are blue can be seen directly through the resin and the plasticized muscles.

The invention discloses a sulfate radical titration method. The sulfate radical titration method comprises steps as follows: a sample containing sulfate radical is taken, a sample solution is prepared, a MgCl2 solution is added, the mass ratio of Mg<2+> to SO4<2-> is 0.27-7.20, 250-500 mg / g of an acetylacetone solution with the concentration being 0.5-14.0 ml is added, 5-15 mL of an acetic acid solution is added after the mixture is uniformly mixed, the pH (potential of hydrogen) of the solution is 2.0-3.7, 5-20 mL of alcohol solvents are added, the mixture is uniformly mixed, an alizarin red indicator is dropwise added, the mixture is titrated with a barium chloride solution until the mixture becomes pink, and the content of sulfate radical is calculated according to the use amount of barium chloride. The method can be well applied to analysis of the content of sulfate radical in salt lake brine. Besides, the method has the advantages of accuracy, convenience, rapidness, simpleness and the like.

The invention discloses application of miR-489-3p to preparation of medicines for diagnosing and treating human osteoporosis. According to the technical scheme, the detection of thighbone tissue samples of elderly patients with osteoporosis shows that miR-489-3p is low in expression in human osteoporosis tissue samples and can be used as a marker gene for diagnosing osteoporosis; an analogue and an antagonist of miR-489-3p are designed and prepared, then the analogue and the antagonist of the miR-489-3p are transfected into mice pre-osteoblasts MC3T3-E1 for carrying out a mineralization-alizarin red s dyeing experiment and an ALP dyeing experiment; technical detection of qPCR and Western blot shows that miR-489-3p can accelerate osteocyte differentiation and also can carry out targeted regulation on non-receptor protein-tyrosine-phosphatase6 (PTPN6) molecules. The application of miR-489-3p to preparation of medicines for diagnosing and treating human osteoporosis shows that miR-489-3p can regulate PTPN6 molecules to be used as target sites of action of medicines for treating skeleton system diseases such as osteoporosis.

The invention provides a method for automatically analyzing sulfide in a water sample, comprising the following steps: (1) leading a developing bath and a pushing liquid to enter an analysis flow path so as to form a mixed liquid, leading the mixed liquid to enter an optical flow-through tank, transmitting a signal to a computer processing system by an optical detector, processing the signal to obtain a baseline, and simultaneously leading the sample to enter a sample feeding ring and filling the sample filling ring; (2) leading the sample in the sample feeding ring to enter the analysis stream under the pushing of the pushing liquid, leading the developing bath to enter the analysis flow path so as to generate developing reaction with the sample during the mixing, leading the formed mixed liquid to enter the optical flow-through tank, and transmitting the signal to the computer processing system by the optical detector so as to process the signal and obtain the sample spectrogram; (3) using a series of standard samples with known sulfide concentration to replace the samples, and repeating the step (1) and the step (2) to obtain a series of sample spectrogram; and (4) comparing the sample spectrogram with the sample with the standard sample spectrogram, and calculating the sulfide content in the sample. The developing bath is a solution that is prepared by alizarin red S and Britton-Robinson buffer solution.

The invention discloses a method for quantitatively analyzing the content of alizarin red through a fluorescence quenching method. According to the method, nitrogen-doped graphene quantum dots are adopted as a fluorescent probe; on the basis of a characteristic that the fluorescence intensity of the nitrogen-doped probe is weakened with the increase of the concentration of alizarin red, high selectivity and high sensitivity detection is performed; and the change value of the fluorescence intensity of the nitrogen-doped graphene quantum dot probe is in a great linear relation with the concentration of the alizarin red, and the correlation coefficient of the fluorescence intensity of the nitrogen-doped graphene quantum dot probe and the concentration of the alizarin red is 0.992. The methodof the invention has the advantages of simple operation, high sensitivity, good selectivity, and quick detection, and is suitable for field in situ analysis.

The invention discloses preparation and application of a hollow spherical-segment-shaped mesoporous silica / chloroperoxidase nanoreactor. According to the nanoreactor, tetraethoxysilane serves as a silicon source, P123 serves as a template agent, and a compound of n-decane and 1,3,5-trimethylbenzene serves as an additive; a hydrothermal synthesis method is utilized for preparing hollow spherical-segment-shaped mesoporous silica firstly, then the mesoporous silica serves as a fixed carrier for fixedly carrying chloroperoxidase. The preparation method is simple, reaction conditions are mild, the obtained mesoporous silica is in a hollow spherical segment shape, the specific surface area is large, the mesoporous diameter range is about 14-18 nm, unimolecular fixation of the chloroperoxidase can be realized, and certain turnover space is reserved for the chloroperoxidase. The hollow spherical-segment-shaped mesoporous silica / chloroperoxidase nanoreactor shows good degradation capacity on alizarin red, the degradation rate still can reach 100% after 8 times of use, the high temperature to which the nanoreactor is resistant is increased to about 50 DEG C, and the acid-base resistance is also improved.

The invention relates to a marking method suitable for acanthopagrus schlegelii juveniles. The method includes the following steps: (1) preparing multiple to-be-marked acanthopagrus schlegelii juveniles with body lengths of 3-5 centimeters; (2) preparing a fluorescent labeling dye into a mother solution of 2,000 mg / L for later use; (3) continuously adding sodium bicarbonate to adjust the pH valueof the solution to 7.5-7.8 during a mother solution preparing process; (5) preparing multiple dipping containers with appropriate sizes; (6) preparing a dipping marking solution of 100-400 mg / L with the mother solution; (7) putting the acanthopagrus schlegelii juveniles with a density of 0.5 per / L in the dipping marking solution; and (8) using clean seawater to wash the dye liquor on the acanthopagrus schlegelii juveniles after the dipping finishes. The tracking technical problem of the acanthopagrus schlegelii juveniles during proliferation and releasing after the acanthopagrus schlegelii juveniles are released can be solved; and marking effects can be guaranteed by taking alizarin red as the fluorescent labeling dye, and thus, efficient, fast and safe dipping marking can be performed onthe acanthopagrus schlegelii juveniles, and marking quality can be guaranteed in a period of time.

The invention relates to a method for marking larimichthys crocea by using alizarin red. The method comprises the steps that 1, an alizarin red is prepared for mother solution preparation, a mother solution is diluted to reach application concentration, then the salinity is regulated with refined salt and the like to reach 1.5% or above, and an artificial seawater dye liquor is obtained; 2, larimichthys crocea fry to be dyed is soaked in the dye liquor for dyeing, the fry is transferred to natural seawater for rinsing after dyeing is completed, and then larimichthys crocea marked with the alizarin red is obtained. By the adoption of the method, the influence of calcium ions on alizarin red dissolution and precipitation are avoided, the effectively concentration and a dip dyeing effect of the alizarin red are improved, and the problem of massive dead due to the fact that suspending precipitates easily block the gills of the larimichthys crocea is solved.

The invention discloses a method for detecting sesame oil adulteration, and relates to the technical field of sesame oil quality monitoring. The method specifically comprises the following steps: adding standard sesame oil into a mixed system prepared by mixing polyphenol dyes and zinc ions for reaction, and recording color change as a standard color; adding a to-be-detected sesame oil sample into a mixed system prepared by mixing polyphenol dyes and zinc ions for reaction, recording color change, and comparing the color change with the standard color; and judging whether the sesame oil is adulterated according to the color difference. According to the invention, based on the coordination effect between Zn<2+> and compounds in the sesame oil, the weak complexing effect between Zn<2+> and polyphenol dyes (alizarin red S, bromo-pyrophenol red, pyrogallol red and catechol purple) can be destroyed, and the color recovery of the polyphenol dyes is realized; and the method is of great significance in protecting health and benefits of consumers, detecting and identifying related departments and maintaining a good market environment.

The invention relates to alizarin red pH response color-changing fibers and a preparation method thereof. According to the preparation method, alizarin red with the dry weight accounting for 0.05%-2% of that of polyacrylonitrile is added into spinning liquid taking 58%-60% sodium sulfocyanate (NaSCN) aqueous solution as a solvent and containing 11%-14% of polyacrylonitrile for wet spinning. The prepared fibers are uniform in coloring and excellent in color fastness, and have weaving mechanical properties and obvious pH response color changing performance.

The invention discloses a method for producing novel energy storage material with organic dye waste and application of the method; nitrogen-bearing dye MB (methylene blue) and anthraquinone dye ARS (alizarin red S) are used as raw materials, mesoporous carbon (CMK-3) is used as an adsorbent, and MB / CMK-3 and ARS / CMK-3 composite electrode material is prepared and is applied to cathode materials for lithium-ion batteries and other batteries, and capacitors. The method comprises the specific steps of (1) dissolving organic dye waste to obtain waste liquid; (2) extracting organic dye from the waste liquid; (3) combining the organic dye with a double-electrode-layer capacitor active material to obtain a composite electrode; (4) fitting the composite electrode in an energy storage device. The method has simple preparation process and good repeatability; the raw materials have rich reserves and low cost; the common organic dye waste in sewage is used as an active material for energy storage materials, environmental pollution can be relieved, and construction of the novel energy storage material is achieved; in addition, the prepared composite electrode has high specific capacity and good circulating performance.

The invention provides a membrane electrode and a preparation method thereof, a sensor by employing a membrane electrode, an electrochemical workstation and application thereof. The membrane electrodecomprises an electrode and a modified membrane on the surface of the electrode, the modified membrane is a mixture of chitosan and alizarin red S; the modified membrane of the chitosan and the alizarin red S is deposited at the surface of the electrode to allow the obtained membrane electrode to have high sensitivity, high sensing effect and have responsiveness to the current change; the preparation method of the membrane electrode is simple, easy to obtain raw materials, low in cost and easy to implement and can be suitable for industrial large-scale production application; and the sensor comprising the membrane electrode is high in detection sensitivity and good in accuracy; the electrochemical workstation comprising the sensor can achieve the qualitative and quantitative detection of aluminum ions, and is high in sensitivity, small in relative standard deviation and high in accuracy.

The invention discloses a tiopronin concentration determination fluorescence sensor and a preparation method thereof. After hydrotalcite is delaminated, a laminate has positive charges, a guest molecule alizarin red-boric acid system has negative charges, and the hydrotalcite laminate and the guest molecule are self-assembled layer by layer through an electrostatic attraction layer to form a super-molecular structure system and then to be prepared into films; a molecular structure of boric acid is changed by adjusting the layer quantity of the thin films, the concentration of boric acid and the pH value of an alizarin red-boric acid solution system in an assembling process; and a fluorescence sensor thin film with high fluorescence intensity and high stability is obtained through optimized treatment. The fluorescence sensor thin film has the advantages that the preparation method is simple, the cost is low, and quantitative detection of tiopronin is realized. The fluorescence sensor provided by the invention can be used for detecting concentration of tiopronin conveniently and rapidly, and is high in sensitivity and low in detection limit; in the detection process, the sensor does not pollute a system to be detected, and has no reagent loss; and regeneration and repeated utilization of the sensor can be realized.

The invention discloses a subcutaneous alizarin red S marking method for sepiella maindroni. The method comprises the following steps: firstly, anesthesia step: selecting sepiella maindroni to be marked into MS-222 anesthetic solution with a concentration of 40ppm to 70ppm for 0.5 to 4 minutes; secondly, marking step: injecting dyeing liquor into the inner shell of the sepiella maindroni treated in step one with an injector. Because the MS-222 anesthetic solution is used for performing anesthesia processing to the sepiella maindroni, the sepiella maindroni basically has no stress when being injected with dyeing liquor, thereby being convenient for operation and reducing the hurt to the sepiella maindroni. The special marking reagent for the subcutaneous alizarin red S marking method for sepiella maindroni comprises 1 part of alizarin red S powder and 4-8 parts of yoghourt according to part by weight, marks can be formed in relatively concentrated subcutaneous areas and are more highly identifiable than the ordinary dyeing marks. In addition, by using the method, after being resuscitated, the sepiella maindroni is not required to be raised temporarily to remove the floating color but can be freed immediately.

The invention discloses a method for quantitative analysis of sulphate radical. The method for quantitative analysis of sulphate radical comprises the following steps: controlling alcohol content in a titration system to be 5-35% and controlling the pH value to be 2.0-3.7, adding 0.000491-0.003275% of an alizarin red indicator into the titration system, and titrating barium chloride solution to an end point at a speed of 0.5-3.0 drops / s. The method for quantitative analysis of sulphate radical has the advantages that 19-103mg sulphate radical can be accurately analyzed, and thus analysis accuracy is within 0.3%; and the method for quantitative analysis of sulphate radical is simple and easy to grasp, influence of interfering ion is eliminated, and measurement range of the existing sulphate radical constant analytical method is expanded, so that a new method is provided for quantitatively analyzing content of sulphate radicals, especially sulphate radical content in a complex salt system.

The invention discloses a preparation method of an alizarin red adsorbent, a product and an application. The preparation method of the adsorbent comprises the steps that a peanut shell is cleaned, baked to the constant weight, crushed and sieved into powder with the particle size less than 100 meshes; epoxy chloropropane and an NaOH solution are added; a reaction is performed for 120min at 80 DEG C; solid-liquid separation is performed; and a residue is collected, washed to be neutral and baked to the constant weight at 50-70 DEG C. The prepared adsorbent is more in aperture and more in surface active component, can improve the physical strength of the peanut shell, has a good adsorption effect on alizarin red dye, and has an adsorption rate exceeding 30%; and the method is used for removing the alizarin red dye, is simple to operate and low in cost, has no secondary pollution, and has an industrial prospect.

The invention relates to a method for measuring the content of calcium sulfate in desulfurized gypsum of a coal-fired power plant, which comprises the following steps: (1) drying the desulfurized gypsum in the sun, grinding, sieving and drying, adding hydrogen peroxide, demineralized water and cation exchange resin, stirring to adjust the pH value, stirring, filtering with filter paper, and fixing the volume to obtain a desulfurized gypsum oxidation solution; (2) transferring the desulfurized gypsum oxidation solution, adding absolute ethyl alcohol and an alizarin red S indicator, uniformly mixing, titrating with a barium chloride standard solution, slowly generating turbid substances in a yellow solution, shaking while titrating, determining the titration end point when reddish color appears, and recording the volume of the consumed standard solution; and (3) substituting the volume of the consumed standard solution into a calculation formula, and calculating to obtain the content of calcium sulfate. The method has the advantages of simple operation steps, good repeatability of detection results, high accuracy, high efficiency, rapidness, low pollution, low drug consumption and low cost, meets the requirements of thermal power plant detection laboratories, and has a wide application range.

The invention discloses polymer microspheres with phenylboronic acid groups loaded on the surface as well as a preparation method and application of the polymer microspheres. Firstly, 4-vinylphenylboronic acid (4-VPBA) and acrylic acid (AA) are subjected to RAFT solution polymerization for preparing a block type macromolecular RAFT reagent P (4-VPBA) m-b-AAn; the macromolecular RAFT reagent is used as a stabilizer, through an RAFT photodispersion polymerization method together with a polymerization monomer and a photoinitiator, phenylboronic acid groups are successfully introduced to the surface of microspheres so that the polymer microspheres with the phenylboronic acid groups loaded on the surfaces are simply and efficiently prepared in one step. The polymer microspheres provided by theinvention have a range of 100 nm to 1 [mu] m and good monodispersity. The phenylboronic acid polymer microspheres have a specific adsorption effect on a compound containing an ortho-hydroxyl structure, such as Alizarin Red S dyes (ARs), and are widely applied to the fields of plant component separation, dye adsorption, analysis and detection and the like.

The invention relates to the technical field of carbon nanodot materials, in particular to fluorescent carbon nanodots as well as a preparation method and application thereof. The preparation method provided by the invention comprises the following steps of: mixing corn straw powder with water to obtain a corn straw powder dispersion liquid; carrying out water liquefaction treatment on the corn straw powder dispersion liquid, and filtering to obtain liquefied liquid; and filtering the liquefied liquid by using a microfiltration membrane, and dialyzing to obtain the fluorescent carbon nanodots. According to the invention, the corn straw is used as a carbon source, so that efficient utilization of waste biomass is realized; and meanwhile, the prepared fluorescent carbon nanodots have high selectivity and sensitivity to alizarin red. Meanwhile, the preparation method is simple, convenient, efficient and rapid, no chemical reagent is used in the preparation process, and the development concept of green chemistry is met.

The invention provides melamine test paper and a preparation method thereof, and a method for detecting the melamine. The melamine test paper is prepared by the following steps of: preparing alizarin red, methylene blue and andlauryl sodium sulphate into an aqueous solution, wherein the concentration of each substance in the aqueous solution is: 0.0167 mol / L of the alizarin red, 2.10*10<-5> mol / L of the methylene blue and 0.015 mol / L of the andlauryl sodium sulphate; immersing filter paper into the aqueous solution, taking out and drying; and shearing into strips to obtain the melamine test paper. When the melamine test paper is in use, the method for detecting the melamine comprises the steps of: adding EDTA (Ethylene Diamine Tetraacetic Acid) solid powder into the to-be-detected liquid cream which is prepared from milk or by mixing milk powder; evenly agitating and heating and micro boiling for 5 min; statically placing and depositing; immersing the test paper into the liquid cream liquid supernatant; and wetting and then extracting out. The invention has the advantages of simple using method, and high detecting speed.

The invention discloses a TiO2 immobilized enzyme reactor and application thereof. By using dopamine-modified TiO2 as a carrier and glutaraldehyde as a cross-linking agent, chloroperoxidase or horseradish peroxidase is immobilized on a surface of the carrier through a covalent bonding action to prepare the TiO2 immobilized enzyme reactor. A preparation process is simple and low in cost; the obtained enzyme reactor has high-efficient catalytic degradation capability on dyes (crystal violet, crocein orange G, alizarin red and the like) and 2,4-dichlorophenol, the degradation rate can reach nearly 100 percent within extremely short time, and a repeated use effect is good.

The invention relates to a method for separating and purifying an o-hydroxyl compound, and belongs to the field of natural product separation and purification. The method comprises the steps that firstly, alizarin red and K2HPO4 are added into a colorimetric tube, then phenylboronic acid functionalized PEO20PPO60PEO20 is added, the solution is diluted with distilled water, full shaking is carried out, and the mixture is mixed to be uniform; the mixture is poured into a flotation column after still standing, then normal propyl alcohol is added, gas flowing speed is regulated, and flotation is started; after the system is divided into two phases, the volume of the upper phase is recorded, and the alizarin red concentration in the upper phase is measured. The double aqueous phase flotation system is set up for flotation separation of o-hydroxyl compound alizarin red; synthesized phenylboronic acid functionalized PEO20PPO60PEO20 is added to serve as a complementing agent and can be effectively combined with alizarin red, meanwhile, functionalized PEO20PPO60PEO20 can be effectively adsorbed on a hydrophobic bubble interface, and flotation separation of water-soluble alizarin red is achieved; obtained alizarin red is large in the enrichment factor and high in purity, and the yield of the product can be increased.