11 results about "ALIZARIN RED" patented technology

The invention provides application of linariin-6-O-glucoside in preparation of a product for preventing and treating osteoporosis, and relates to the field of biological medicine. According to the invention, dyeing experiments of alkaline phosphatase, alizarin red and tartaric acid resistant acid phosphatase prove that the sesaminin-6-O-glucoside has affinity for FPPS receptors, has the capability of promoting bone marrow mesenchymal stem cells to form bone cells, is superior to that of bisphosphate, and can inhibit the generation of osteoclasts; microCT (micro computed tomography) detection results show that the sesaminin-6-O-glucoside has the effect of relieving the osteoporosis and can promote the formation of bone trabecula. According to the application disclosed by the invention, it is proved for the first time that the sesaminin-6-O-glucoside has the effect of resisting osteoclast formation, and meanwhile, the sesaminin-6-O-glucoside also has a better osteogenesis effect, so that a basis is provided for developing the sesaminin-6-O-glucoside as a potential medicine for treating osteoporosis.

This invention provides a standardized preparation method and kit for double-stained transparent specimens of fish skeletons. Orthogonal experiments were designed for fish of different skeleton types (cartilaginous / boneless) and body lengths (small 5-7cm, medium 7-15cm, large 15-25cm), using factors such as KOH concentration and treatment time. Staining effect, transparency, and tissue integrity were used as indicators. Statistical analysis was used to establish an optimal parameter matrix database covering fixation, transparentization, and staining steps. During preparation, the database can be queried based on fish species and body length to obtain differentiated treatment parameters, achieving standardized operation. This invention also provides a kit containing staining stock solution, basic reagents, and a parameter query unit. This invention transforms double-stained skeleton preparation from experience-dependent to data-driven, significantly improving repeatability and success rate, and is suitable for scientific research, teaching, and demonstration.

The invention relates to the technical field of biochemical medicine, in particular to a dry chemical qualitative detection method and a kit for detecting seminal plasma fructose. The method comprises the following steps: by adopting a room-temperature competitive color development system constructed by 3-quinoline boronic acid and alizarin red S, mixing a sample with a special diluent, and dropwise adding the mixture into the kit pre-loaded with test paper; and the judgment can be completed by comparing colors with naked eyes. The operation threshold and the cost limitation of equipment such as high performance liquid chromatography are avoided; the reaction is carried out under mild conditions, and strong corrosive reagents such as concentrated hydrochloric acid are not used, so that the operation safety is guaranteed; the sample treatment only needs simple dilution, and tedious pretreatment steps such as deproteinization, heating or derivatization are not needed, so that the detection efficiency is improved; the selected 3-quinoline boric acid, alizarin red S and auxiliary materials are conventional easily available chemical reagents, and the problem of obtaining special enzyme raw materials is solved. The technical effects of simplicity, convenience, quickness, safety, low cost and easiness in popularization are realized.

The invention provides a dual-emission carbon dot probe with a dynamic adjustable range for glutathione as well as a preparation method and application of the dual-emission carbon dot probe. The carbon dot probe is synthesized from alizarin red, carbon powder and ethylenediamine through a hydrothermal method, and the carbon dot probe shows concentration-dependent nonlinear fluorescence response in the presence of glutathione. By adjusting the concentration of the phosphate buffer solution, the detection parameters of the probe can be continuously and adjustably changed, and the wide-range detection of glutathione from 0.46 mu M to 3.2 mM is realized. The carbon dot probe has the advantages of high sensitivity, high selectivity, simplicity and convenience in operation, low cost and the like, and can be widely applied to the fields of environmental monitoring, food safety, biomedical diagnosis and the like.

The present invention discloses a preparation method and application of an indicator displacement sensor array for tea polyphenol identification. The array is prepared by using two arylboronic acids, 1-naphthaleneboronic acid (NapB) and 1-pyreneboronic acid (PyrB), as probes, and catechol-containing Alizarin Red S (ARS) and catechol violet (PV) as indicators. Based on the indicator displacement method (IDA) mechanism, a 2×2 colorimetric sensor array is designed and constructed for detecting and identifying different types of tea polyphenols, namely gallic acid, methyl gallate, epicatechin, epigallocatechin, epigallocatechin gallate, and epicatechin gallate. The binding force between tea polyphenols and the boronic acid probe is stronger than that of the indicator. Different tea polyphenols have different binding abilities to the probe and different abilities to displace the indicator, resulting in different colorimetric responses of the IDA sensor array to tea polyphenols. In addition, the colorimetric sensing array was combined with principal component analysis (PCA) to successfully distinguish different types of tea polyphenols.

The invention discloses a preparation method of a co-immobilized laccase-mediator system, which comprises the following steps: mixing and oscillating a laccase solution and a 2-methylimidazole solution, adding a manganese chloride solution, oscillating and uniformly mixing, reacting the obtained mixed solution in a shaking table for a preset time, sequentially adding p-coumaric acid and 2, 4-dihydroxy-2-methylimidazole, reacting for a preset period of time, filtering, washing, and drying to obtain the co-immobilized laccase-mediator system. The preparation method comprises the following steps: adding a laccase-mediator system MnMOF (at) Lac5 (at) M (at) PEG (Polyethylene Glycol) into a reaction kettle, carrying out oscillation reaction on the laccase-mediator system MnMOF (at) Lac5 (at) M (at) PEG and 2, 2-nitrogen-bis (3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt ABTS for 3-5 hours, then adding polyethylene glycol PEG, centrifuging and washing to obtain the co-immobilized laccase-mediator system MnMOF Compared with free enzyme, the PEG modified laccase-mediator co-immobilization system adopted by the invention has the advantages that the enzyme activity is improved by more than two times, and excellent reusability is shown; under the condition of 30 DEG C, the immobilized laccase-mediator system can realize 100% degradation of alizarin red within 15 minutes, and a green and efficient treatment technology is provided for dye degradation; meanwhile, the system has a good application prospect in the fields of sewage treatment, bioremediation and the like.

The present application relates to the application of QKI gene in the preparation of drugs for preventing and treating vascular calcification. The present application discloses the important role of QKI gene in regulating vascular calcification by constructing smooth muscle specific QKI knockout mice. Compared with the control mice, the QKI knockout mice significantly reduce the occurrence of vascular calcification, including the decrease of Runx2 gene expression level, the significant reduction of vascular calcium salt deposition and the decrease of vascular tissue calcium ion. In the in vitro test, it is found that QKI siRNA makes the alizarin red staining of mouse vascular smooth muscle cells under high phosphorus stimulation weaken, the calcium content decreases, and the vascular smooth muscle cell calcification is reduced. It is found that the present application takes QKI gene as the action target to prepare or screen the drug for slowing down the vascular calcification, and the drug has important significance in the treatment of vascular calcification.

The invention aims to provide a preparation method and application of yellow fluorescent carbon dots for detecting mercury ions and biological mercaptans, and belongs to the technical field of fluorescent carbon dots, and the preparation method of the fluorescent carbon dots comprises the following steps: 1) weighing a certain mass of alizarin red S and urea, dissolving in secondary water, and carrying out ultrasonic treatment to obtain a uniformly mixed solution; 2) transferring the solution into a hydrothermal reaction kettle, reacting for 4-10 hours at 150-220 DEG C, standing and cooling to room temperature after the reaction is stopped, centrifuging to remove insoluble substances, taking supernate, and dialyzing for at least three days in a glass container through a dialysis bag of 500-1000 Da to obtain a pure carbon dot aqueous solution; 3, the carbon dot aqueous solution is freeze-dried, and then the yellow fluorescence-emitting carbon dots are obtained.The prepared yellow fluorescence carbon dots can be used for sequentially detecting mercury ions and biological mercaptan and are good in selectivity and high in sensitivity.

1. Name of the designed product: Ceramic cup (blue and white with alizarin red color and joyful small stove type of branch and month of rose). 2. Use of the designed product: holding tea. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo best indicating the design points: perspective view.

The present application relates to the technical field of dye adsorbent chemistry, and particularly relates to a broad-spectrum anionic dye adsorbent as well as a preparation method and application thereof, the adsorbent is a novel high-performance PEI / SA adsorbent, can efficiently adsorb various anionic dyes, has a large adsorption capacity, a high adsorption rate, is biodegradable, and can be recycled. The adsorbent provided by the present application has a simple preparation process, does not need heating, is completed in one step, and has no pollutant emission in the preparation process; does not need further cleaning, purification, and can be directly used after product drying; and product preparation does not need special equipment. The present application solves the problem of a complex and lengthy preparation process of traditional adsorbents. The present application has low investment cost, strong practicability, and is easy to realize industrialization and popularization. The adsorbent provided by the present application can be used for adsorbing Congo red, amino black 10B, acid blue 25, acid magenta, methyl orange, acid green 25, sunset yellow, indigo carmine, alizarin red S, direct red 7, titan yellow, and methyl blue.

According to the invention, alizarin red and calcium salt are subjected to a chromogenic reaction to generate an orange red compound, osteogenic differentiation is determined by observing calcium nodules deposited outside cells induced by osteogenesis, a CPC solution is added to dissolve the orange red compound, and the absorbance OD value is measured under a certain wavelength to carry out quantitative detection on osteogenic differentiation. The conversion from qualitative detection to relative quantitative detection of osteogenic differentiation is realized.