Method for improving osteogenic differentiation efficiency of human amniotic mesenchymal stem cells and application thereof

A technology for stem cell and osteogenic differentiation, applied in biochemical equipment and methods, animal cells, vertebrate cells, etc., can solve the problem of undiscovered human-derived factor stem cells, etc., to improve the efficiency of osteogenic differentiation and promote expression. and activity, the effect of avoiding the difficulty of osteoblasts
CN106497871AInactive Publication Date: 2017-03-15AFFILIATED HOSPITAL OF ZUNYI MEDICAL COLLEGE
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
AFFILIATED HOSPITAL OF ZUNYI MEDICAL COLLEGE
Publication Date
2017-03-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for improving osteogenic differentiation efficiency of human amniotic mesenchymal stem cells. The method adds an induction composition in the human amniotic mesenchymal stem cells, wherein the composition comprises 0.1-2g / L of hyaluronic acid, 0-200mg / L of prostaglandin receptor-2 selective agonist, 1-100nmol / L of dexamethasone, 0-10nmol / L of beta-glycerophosphate and 1-100mg / L of ascorbic acid. Compared with a conventional inducing method, the osteogenic differentiation efficiency of the human amniotic mesenchymal stem cells can be significantly improved, expression and activity of alkaline phosphatase are promoted, and expression of osteogenesis-related genes of RunX2, Osx, BSP, Ocn, ALP, Col1a1 and the like and formation of mineralized calcium nodules are enhanced. The method for improving the osteogenic differentiation efficiency of the human amniotic mesenchymal stem cells can be applied in osteogenic differentiation of the human mesenchymal stem cells, and applied in bone diseases of bone defects, bone trauma and the like.
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Description

technical field

[0001] The invention belongs to the technical field of stem cell biotechnology and bone tissue engineering, and relates to a method and application for improving the osteogenic differentiation efficiency of human amniotic mesenchymal stem cells. Background technique

[0002] Bone damage or defect due to trauma, infection, degenerative disease, tumor resection and other reasons is a common and frequently-occurring disease in orthopedic diseases. Taking osteoarthritis as an example, there are currently about 150 million patients in my country. WHO estimates that by 2025, there will be more than 800 million patients worldwide. The number is second only to cardiovascular-related diseases, and it has become an important disease that seriously affects human health. At present, the repair and functional reconstruction of bone defects has become a major problem in the medical field because traditional treatment methods are difficult to achieve ideal results. In rece...

Claims

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