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A medium for promoting differentiation of mesenchymal stem cells

A technology of high-quality stem cells and medium, applied in the field of stem cells, can solve the problems of low stem cell differentiation efficiency, achieve the effect of improving efficiency and promoting bone cell differentiation

Active Publication Date: 2020-09-01
朗姿赛尔生物科技(广州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem solved by the present invention is to solve the problem of low differentiation efficiency of stem cells to bone cells, and to provide a medium for promoting the differentiation of mesenchymal stem cells

Method used

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  • A medium for promoting differentiation of mesenchymal stem cells

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Embodiment 1

[0021] A medium for promoting differentiation of mesenchymal stem cells, including insulin 7μg / ml, transferrin 0.04μg / ml, BMP-4 0.2ng / ml, FGF-2 9ng / ml, growth hormone 5ng / ml, glutathione Peptide 180μg / ml, L-glutamine 0.8mM, β-mercaptoethanol 70μM, sodium pyruvate 0.4mM, non-essential amino acid 0.12mM, nano-ferric oxide 12μM, and the balance was DMEM medium. The nanometer ferric oxide is modified nanometer ferric oxide. The preparation method of the modified ferric oxide is as follows: take 12 parts by mass of nanometer ferric oxide, 3 parts by mass of graphene oxide, and 5 parts by mass of urea; fully grind the graphene oxide and urea and roast in an argon atmosphere After 10 hours, wash with dilute hydrochloric acid, and dry at 70 degrees Celsius for 12 hours to obtain nitrogen-doped graphene; mix nano-ferric oxide with nitrogen-doped graphene evenly, add 20 times the amount of absolute ethanol, and ultrasonicate for 1 hour. Stirring under the condition of 500w mercury lamp...

Embodiment 2

[0024] A medium for promoting the differentiation of mesenchymal stem cells, including insulin 6μg / ml, transferrin 0.03μg / ml, BMP-4 0.1ng / ml, FGF-2 8ng / ml, growth hormone 4ng / ml, glutathione Peptide 160μg / ml, L-glutamine 0.5mM, β-mercaptoethanol 60μM, sodium pyruvate 0.1mM, non-essential amino acid 0.1mM, nano-ferric oxide 10μM, and the balance was DMEM medium. The nanometer ferric oxide is modified nanometer ferric oxide. The preparation method of the modified ferric oxide is as follows: take 10 parts by mass of nanometer ferric oxide, 1 part by mass of graphene oxide, and 3 parts by mass of urea; fully grind the graphene oxide and urea, and roast in an argon atmosphere After 10 hours, wash with dilute hydrochloric acid, and dry at 60 degrees Celsius for 12 hours to obtain nitrogen-doped graphene; mix nano-ferric oxide and nitrogen-doped graphene evenly, add 20 times the amount of absolute ethanol, and ultrasonicate for 1 hour. Stirring under the condition of 500w mercury la...

Embodiment 3

[0026]A medium for promoting the differentiation of mesenchymal stem cells, including insulin 8μg / ml, transferrin 0.05μg / ml, BMP-4 0.3ng / ml, FGF-2 10ng / ml, growth hormone 6ng / ml, glutathione Peptide 200μg / ml, L-glutamine 2mM, β-mercaptoethanol 80μM, sodium pyruvate 0.5mM, non-essential amino acid 0.15mM, nano-ferric oxide 15μM, and the balance was DMEM medium. The nanometer ferric oxide is modified nanometer ferric oxide. The preparation method of the modified ferric oxide is as follows: take 15 parts by mass of nanometer ferric oxide, 5 parts by mass of graphene oxide, and 8 parts by mass of urea; fully grind the graphene oxide and urea and roast in an argon atmosphere After 10 hours, wash with dilute hydrochloric acid, and dry at 80 degrees Celsius for 12 hours to obtain nitrogen-doped graphene; mix nanometer ferric oxide with nitrogen-doped graphene evenly, add 20 times the amount of absolute ethanol, and ultrasonicate for 1 hour. Stirring under the condition of 500w mercu...

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Abstract

The invention relates to the field of stem cells, provides a culture medium for promoting differentiation of mesenchymal stem cells, and is used to solve the problem of low differentiation efficiencyof stem cells into bone cells. The culture medium for promoting differentiation of mesenchymal stem cells provided by the invention comprises 6-8 [mu]g / ml of insulin, 0.03-0.05 [mu]g / ml of transferrin, 0.1-0.3 ng / ml of BMP-4, 8-10 ng / ml of FGF-2, 4-6 ng / ml of growth hormone, 160-200 [mu]g / ml of glutathione, 0.5-2 mM of L-glutamine, 60-80 [mu]M of beta-mercaptoethanol, 0.1-0.5 mM of sodium pyruvate, 0.1-0.15 mM of non-essential amino acids, 10-15 [mu]M of nano ferric oxide, and the balance a DMEM culture medium. No biological serum is added into the culture medium, and a nano oxide is introduced at the same time, so that the culture medium can significantly promote differentiation of the mesenchymal stem cells into bone cells.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to a culture medium for promoting differentiation of mesenchymal stem cells. Background technique [0002] Stem cells are a kind of cell type with self-renewal ability and multi-directional differentiation potential. It is the first cell in the process of human newborn cells from proliferation, migration, differentiation to maturity. The study of stem cells has become one of the most challenging and attractive fields in biology. Stem cells can be divided into embryonic stem cells and adult stem cells according to their source. Mesenchymal stem cells (MSCs, mesenehymal stem cells) are one of the adult stem cells. As a type of adult stem cells with self-renewal ability, mesenchymal stem cells can differentiate into various cell types. It can not only differentiate into a variety of mesenchymal-derived cell lineages, such as adipocytes, osteoblasts, chondrocytes, myocytes, etc., but also d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077C01G49/06C01B32/198B82Y30/00
CPCB82Y30/00C01B32/198C01G49/06C12N5/0654C12N2500/12C12N2500/24C12N2500/30C12N2500/32C12N2500/44C12N2501/115C12N2501/155C12N2501/305C12N2501/33C12N2501/998C12N2506/1346
Inventor 陈忠平
Owner 朗姿赛尔生物科技(广州)有限公司
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