Culture medium for promoting mesenchymal stem cell osteogenic differentiation

A technology of osteogenic differentiation and stem cells, which can be used in cell culture active agent, culture process, tissue culture and other directions, and can solve problems such as troubles

Inactive Publication Date: 2020-07-07
朗姿赛尔生物科技(广州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Stem cell transplantation to treat various diseases has become a hot spot in medical research today, but how to distinguish donor cells from recipients after transplantation and observe their survival and migration in vivo has always been a bottleneck that plagues its clinical application

Method used

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  • Culture medium for promoting mesenchymal stem cell osteogenic differentiation

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Effect test

Embodiment 1

[0022] A medium for promoting osteogenic differentiation of mesenchymal stem cells, including insulin 10 μg / ml, transferrin 0.08 μg / ml, BMP-4 0.1ng / ml, FGF-2 6ng / ml, growth hormone 2ng / ml, 120 μg / ml of glutathione, 2 mM of L-glutamine, 45 μM of β-mercaptoethanol, 2 mM of sodium pyruvate, 0.1 mM of non-essential amino acids, 5 μM of nano-iron trioxide, and the balance is DMEM medium. The nanometer ferric oxide is modified nanometer ferric oxide. The preparation method of the modified ferric oxide is as follows: take 12 parts by mass of nano ferric oxide, 24 parts by mass of ethyl orthosilicate, 36 parts by mass of polyvinylidene fluoride, 0.4 parts by mass of ruthenium trichloride, and 0.4 parts by mass of selenium trichloride. 0.2 parts by mass of sodium, 6 parts by mass of tiopronin; disperse nano-ferric oxide in 40 times the amount of anhydrous ethanol, add ethyl orthosilicate, sonicate for 1.5h, heat up to 60°C, adjust pH to alkali the first product was mixed with polyviny...

Embodiment 2

[0025]A medium for promoting osteogenic differentiation of mesenchymal stem cells, comprising insulin 8 μg / ml, transferrin 0.05 μg / ml, BMP-4 0.05ng / ml, FGF-2 5ng / ml, growth hormone 1ng / ml, 100 μg / ml of glutathione, 1 mM of L-glutamine, 40 μM of β-mercaptoethanol, 1 mM of sodium pyruvate, 0.05 mM of non-essential amino acids, 2 μM of nano-iron trioxide, and the balance of DMEM medium. The nanometer ferric oxide is modified nanometer ferric oxide. The preparation method of the modified ferric oxide is as follows: taking 10 parts by mass of nano ferric oxide, 20 parts by mass of ethyl orthosilicate, 30 parts by mass of polyvinylidene fluoride, 0.3 parts by mass of ruthenium trichloride, selenium 0.1 parts by mass of sodium, 5 parts by mass of tiopronin Disperse nano-ferric oxide in 40 times the amount of anhydrous ethanol, add ethyl orthosilicate, ultrasonicate for 1 h, heat up to 60 ° C, adjust the pH to be alkaline, Incubate the reaction for 12h, filter, take the filter residu...

Embodiment 3

[0027] A medium for promoting osteogenic differentiation of mesenchymal stem cells, including insulin 12 μg / ml, transferrin 0.2 μg / ml, BMP-4 0.2ng / ml, FGF-2 8ng / ml, growth hormone 3ng / ml, 150 μg / ml of glutathione, 3 mM of L-glutamine, 50 μM of β-mercaptoethanol, 3 mM of sodium pyruvate, 0.15 mM of non-essential amino acids, 10 μM of nano-ferric oxide, and the balance is DMEM medium. The nanometer ferric oxide is modified nanometer ferric oxide. The preparation method of the modified ferric oxide is as follows: taking 15 parts by mass of nano ferric oxide, 30 parts by mass of ethyl orthosilicate, 40 parts by mass of polyvinylidene fluoride, 0.5 parts by mass of ruthenium trichloride, and selenium 0.3 parts by mass of sodium, 8 parts by mass of tiopronin; disperse nano-ferric oxide in 40 times the amount of anhydrous ethanol, add ethyl orthosilicate, sonicate for 2 hours, heat up to 60°C, and adjust the pH to be alkaline , the reaction was kept for 12 h, filtered, and the filte...

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Abstract

The invention relates to the field of stem cells, provides a culture medium for promoting osteogenic differentiation of mesenchymal stem cells, and is used for solving the problem of low efficiency ofdifferentiation of stem cells into bone cells. The culture medium for promoting osteogenic differentiation of mesenchymal stem cells provided by the invention comprises 8-12 [mu] g/ml of insulin, 0.05-0.2 [mu] g/ml of transferrin, 0.05-0.2 ng/ml of BMP-4, 5-8 ng/ml of FGF-2, 1-3 ng/ml of growth hormone, 100-150 [mu] g/ml of glutathione, 1-3 mM of L-glutamine, 40-50 [mu] M of beta-mercaptoethanol,1-3 mM of sodium pyruvate, 0.05-0.15 mM of non-essential amino acid, 2-10 [mu] M of nano ferric oxide and the balance of a DMEM culture medium. No biological serum is added into the culture medium, and meanwhile, nano oxide is introduced, so that mesenchymal stem cells can be remarkably promoted to differentiate into bone cells.

Description

technical field [0001] The invention relates to the technical field, in particular to a culture medium for promoting osteogenic differentiation of mesenchymal stem cells. Background technique [0002] Stem cells are a kind of cell type with self-renewal ability and multi-directional differentiation potential. It is the first cell in a series of processes from proliferation, migration, differentiation to maturity of new human cells. Research on stem cells has become one of the most challenging and attractive fields in biology. Stem cells can be divided into embryonic stem cells and adult stem cells according to their sources. Mesenchymal stem cells (MSCs, mesenehymal stem cells) are one of the adult stem cells. As a class of adult stem cells with self-renewal ability, mesenchymal stem cells can differentiate into a variety of cell tissue types. It can not only differentiate into a variety of mesenchymal-derived cell lineages, such as adipocytes, osteoblasts, chondrocytes, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077
CPCC12N5/0654C12N2500/24C12N2500/25C12N2500/30C12N2500/32C12N2500/33C12N2500/44C12N2500/90C12N2501/115C12N2501/155C12N2501/305C12N2501/998C12N2506/1346
Inventor 陈忠平
Owner 朗姿赛尔生物科技(广州)有限公司
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