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Near-infrared light controlled UCNPs carrier as well as preparation method and application thereof
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A near-infrared light and near-infrared technology is applied in the field of biomaterials and biomedicine to achieve good biomedical application prospects
Active Publication Date: 2020-08-21
SOUTH CHINA NORMAL UNIVERSITY
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The control and detection of differentiation of MSCs is the key to its clinical application, but there is currently no method that can control and detect differentiation at the same time
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Embodiment 1
[0047] The invention provides the preparation and application of an upconversion nanomaterial for controlling MSCs directional differentiation and detecting differentiation by near-infrared light, comprising the following steps:
[0048] a) construction of multifunctional UCNPs carrier;
[0049] b) After the multifunctional UCNPs carrier is loaded with ICA, it is used to control the osteogenic differentiation of MSCs with near-infrared light;
[0050] c) The multifunctional UCNPs carrier was used to detect the osteogenic differentiation of MSCs by near-infrared light.
[0051] Wherein, step a) constructs multifunctional UCNPs carrier ;
[0052] The preparation method of the multifunctional UCNPs is:
[0053] (1) Preparation of upconversion rare earth UCNPs core:
[0093] The present invention further explores the detection of MMP13 enzyme by UCNPs carrier in Example 1, the control and detection method of controlling UCNPs nanocomposites to release ICA by near infrared, and detecting the osteogenic differentiation of MSCs.
[0094] First, a control method for near-infrared control of UCNPs nanocomposites to release ICA is provided, including the following steps:
[0095] Step 1: Take 1mg / mL UCNPs nanocomposites and evenly disperse them in PBS, and then use a power of 0.2-1W / cm 2 The near-infrared light irradiation, the irradiation time is 10 to 110 minutes, irradiating for 10 minutes and stopping for 10 minutes, alternately;
[0096] Step 2: After the near-infrared light irradiation is over, centrifuge to collect the supernatant, measure the content of ICA in the supernatant, and calculate the release amount and release rate of ICA under different irradiation power and time conditions according to the ICA concentration standard curve.
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Abstract
The invention provides a near-infrared controlled multifunctional UCNPs carrier, a UCNPs nano-composite and a preparation method and application of the UCNPs carrier. The preparation method comprisesthe following steps: firstly, synthesizing a rare earth UCNPs core doped with yttrium, ytterbium, erbium and thulium through a high-temperature decomposition method; coating the surface of the core with mesoporous silica and modifying azobenzene in the mesoporous silica to obtain UCNP@mSiO2-azo; and modifying RGD polypeptide and MMP13 enzyme digested polypeptide on the surface of the UCNP@mSiO2-azo to obtain the UCNPs carrier capable of controlling directional differentiation of MSCs by near-infrared light and detecting differentiation. According to the UCNPs nano-composite, on the basis of meeting the clinical application of controllable induction of MSCs osteogenic differentiation, the osteogenic differentiation of cells can be detected, and the UCNPs nano-composite has a good biomedicalapplication prospect.
Description
technical field [0001] The invention belongs to the field of biomaterials and biomedicine, and in particular relates to a near-infrared controlled UCNPs carrier, a UCNPs nanocomposite, and a preparation method and application thereof. Background technique [0002] Mesenchymal stem cells (MSCs) are a kind of adult stem cells with multi-directional differentiation potential. Under certain conditions, they can be induced to differentiate into various tissue cells such as osteoblasts, chondrocytes, fat cells, cardiomyocytes and nerve cells. Hotspots in tissue and organ injury repair and regenerative medicine in medicine and clinical medicine. At present, the main problem in the clinical application of MSCs is the low differentiation efficiency or non-directed differentiation to form heterogeneous cells, while the directional induction of MSCs to differentiate into osteoblasts in bone tissueengineering is the key. [0003] At present, the small molecule compound icariin (ICA) i...
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