A fluorescent probe targeting cell membrane surface GABA receptor and its preparation method and application
A fluorescent probe, cell membrane technology, applied in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve problems such as unfavorable imaging of cell membranes, unfavorable development and use, background signal interference, etc., to achieve good photostability, easy to use Modified effect of biocompatibility and high sensitivity
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Embodiment 1
[0029] The preparation process of the GABA receptor fluorescent probe targeting the cell membrane surface of this embodiment is as follows:
[0030]
[0031] The specific preparation method is:
[0032] Accurately weigh 0.0025 mol each of 4-(diethylamino)salicylaldehyde and 4,6-dihydroxy-2-mercaptopyrimidine into a dry round-bottomed flask, then add 10 mL of concentrated sulfuric acid and mix well, and place in an oil bath at 90 °C Condensate and reflux for 6 h under the condition, after cooling to room temperature, add an appropriate amount of ice cubes to dilute, add perchloric acid solution drop by drop under stirring conditions, and then filter with suction to obtain the crude product reddish-brown solid, wash the obtained solid with absolute ethanol Operation to purify it. The mass of the finally obtained solid product was 0.5721 g, so the product yield was 76%. The H NMR spectrum of the product probe is as Figure 9 Shown; C NMR spectrum as Figure 10 shown.
Embodiment 2
[0034] The preparation process of the GABA receptor fluorescent probe targeting the cell membrane surface of this embodiment is as follows:
[0035]
[0036] The specific preparation method is:
[0037] Accurately weigh 0.0025 mol of 4-(diethylamino) salicylaldehyde and 0.00266 mol of 4,6-dihydroxy-2-mercaptopyrimidine into a dry round bottom flask, then add 10 mL of concentrated sulfuric acid and mix well, 90 Condensate and reflux in an oil bath at ℃ for 6 hours. After cooling to room temperature, add an appropriate amount of ice cubes to dilute, add perchloric acid solution drop by drop under stirring conditions, and then filter with suction to obtain a reddish-brown solid of the crude product. Distill the obtained solid with absolute ethanol It is purified by washing operations. The mass of the finally obtained solid product was 0.6022 g, so the product yield was 79.8%.
[0038] Example of implementation effect
[0039] 1. The fluorescence spectrum of the probe in PBS...
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