13 results about "Articular chondrocyte" patented technology

The present application provides an activity analysis detection method for a stress-induced mesenchymal stem cell extract, comprising step one, culturing human primary articular chondrocytes; step two, aspirating the culture fluid, adding an equal volume of a composition to be tested, and incubating, wherein the composition to be tested includes a mesenchymal stem cell extract; step three, then adding an equal volume of a lipopolysaccharide solution with a concentration of 80ng / mL-120ng / mL, continuing to incubate, and collecting a cell lysate, which is used to detect inflammatory cytokines. The present application determines the optimal use concentration and cell incubation time of a mesenchymal stem cell extract (denoted as Alituo) and a lyophilized composition (denoted as Alip) and a lipopolysaccharide solution by measuring cell viability and the levels of inflammatory factors IL-6, IL-1β, and TNF-α. By detecting cell viability and inflammatory factors, the quality of the mesenchymal stem cell extract can be efficiently evaluated, and the activity analysis detection method has high repeatability and good stability.

The invention relates to an ROS-responsive macrophage-chondrocyte dual-targeting nano-micelle and a preparation method and application thereof, HA capable of targeting and highly expressing CD44 cells is taken as a carrier skeleton, chemical coupling with TK and CUR is carried out in sequence, self-assembly is carried out through intermolecular force to form a stable nano-micelle, and drugs JPH203 and KGN are entrapped at the same time, so that efficient loading and targeting delivery are realized. The HA can be specifically combined with RAW264.7 macrophages and articular cartilage cells for highly expressing a CD44 receptor in an inflammatory state, and is actively delivered in a targeting manner. The ROS response characteristic of TK is utilized, oxidative fracture is carried out in an OA high ROS microenvironment, and micelles are triggered to disintegrate and release drugs accurately. The released JPH203 can inhibit an inflammatory mTOR pathway and effectively resist inflammation, and KGN is beneficial to promoting cartilage cell proliferation and effectively collecting and promoting differentiation of mesenchymal stem cells into cartilage cells, so that in-situ cartilage regeneration is realized, and a more efficient solution with low side effects is provided for osteoarthritis treatment.

The invention discloses a parasitic loranthus exosome-like nano vesicle and application thereof in preparation of a medicine for treating temporomandibular joint osteoarthritis. The taxillus sutchuenensis exosome-like nano-vesicles are prepared by combining a differential ultracentrifugation method with an ultrafiltration method. Researches show that the taxillus sutchuenensis exosome-like nano-vesicles can treat temporomandibular joint osteoarthritis through the following aspects: 1) an anti-inflammatory function: effectively reducing expression of inflammatory factors; 2) a cartilage degeneration inhibition function: blocking a cartilage matrix degradation pathway, and slowing down cartilage tissue damage; the taxillus sutchuenensis exosome-like nano-vesicles have the advantages that the taxillus sutchuenensis exosome-like nano-vesicles have obvious protection and repair effects on relieving temporal-mandibular joint osteoarthritis related bone and cartilage damage, and accordingly the taxillus sutchuenensis exosome-like nano-vesicles have good application prospects.

This invention relates to a compound nutritional composition and its application. The compound nutritional composition comprises the following components: 1 part by weight of turmeric; 0.002-5 parts by weight of enzymatically hydrolyzed bone powder; and 0.02-0.2 parts by weight of cartilage powder containing type II collagen or 0.02-10 parts by weight of collagen peptide powder. The compound nutritional composition provided in this application addresses the specific osteoarthritis issues of athletes. By constructing an articular cartilage cell inflammation model and conducting population testing, the intervention effects of the compound nutritional composition provided in this application on exercise-related oxidative stress, inflammatory response, and pain perception are systematically evaluated. The aim is to overcome the limitations of traditional broad-based formulations and develop precise nutritional solutions that combine component synergy with exercise physiological adaptability, providing athletes with more scientific and effective joint health protection.

The invention relates to a composite nutritional composition and application thereof. The composite nutritional composition comprises the following components in parts by weight: 1 part of turmeric; 0.002 to 5 parts by weight of enzymatically hydrolyzed bone meal; and 0.02 to 0.2 part by weight of cartilage powder containing type II collagen or 0.02 to 10 parts by weight of collagen peptide powder. According to the compound nutritional composition provided by the invention, aiming at the particularity of bone and joint problems of sports people, the intervention effect of the compound nutritional composition provided by the invention on oxidative stress, inflammatory response and pain feeling related to sports is systematically evaluated by constructing an articular cartilage cell inflammation model and testing people; the invention aims to break through the limitation of a traditional general population formula, develop a precise nutrition scheme with component synergy and exercise physiology adaptability, and provide more scientific and effective joint health protection for exercise crowds.

The invention provides an activity analysis and detection method of a stress-induced mesenchymal stem cell extract. The activity analysis and detection method comprises the following steps: step 1, culturing human primary articular cartilage cells; 2, the culture solution is sucked out, a to-be-detected composition with the same volume is added, incubation is conducted, and the to-be-detected composition comprises a mesenchymal stem cell extract; and step 3, then adding a lipopolysaccharide solution with the isovolumetric concentration of 80 ng / mL-120 ng / mL, continuing incubation, and collecting a cell lysis solution which is used for detecting inflammatory cytokines. According to the present invention, the optimal use concentration and the optimal cell incubation time of the mesenchymal stem cell extract (marked as ALITO), the freeze-drying agent composition (marked as ALIP) and the lipopolysaccharide solution are determined by determining the cell viability and the levels of the cell inflammatory factors IL-6, IL-1beta and TNF-alpha, and the cell viability and the inflammatory factors are detected so as to determine the optimal use concentration and the optimal cell incubation time of the mesenchymal stem cell extract (marked as ALITO), the freeze-drying agent composition (marked as ALIP) and the lipopolysaccharide solution; the quality of the mesenchymal stem cell extract can be efficiently evaluated, and the activity analysis and detection method is high in repeatability and good in stability.

The invention provides a 3D synovial cartilage organ chip with a bionic structure and a preparation method and application thereof, and relates to the technical field of organ chip manufacturing. The organ chip comprises a main body; the main body comprises a top layer, a cartilage layer, a joint synovial fluid layer, a synovial membrane and an endothelial channel layer which are arranged in sequence; a cartilage cavity for accommodating articular cartilage cells is formed in the cartilage layer; a synovial fluid cavity used for containing joint synovial fluid is formed in the joint synovial fluid layer. A synovial membrane channel and an immune cell channel are arranged in the synovial membrane and endothelial channel layer in parallel; a permeation zone is arranged between the synovial membrane channel and the immune cell channel; a pressure cavity is formed in the top layer, and an elastic film is arranged on the portion, on one side of the cartilage cavity, of the pressure cavity. According to the scheme, mechanical stimulation is integrated into the organ chip, and in-vivo periodic compression during walking / activity of a human body can be reproduced, so that mechanical-biochemical coupling of a cartilage-synovial membrane-bone interface is simulated more truly, and the similarity of an in-vitro model is improved.

Disclosed herein are methods for treating osteoarthritis may be a one-step arthroscopic procedure and may include detaching synovial mesenchymal stem cells (MSCs) from the synovium using a brush device; covering articular cartilage in an affected joint with a scaffold; and placing concentrated MSC exosomes into the affected joint to stimulate differentiation of synovial MSCs into articular cartilage cells.

Improved compositions and methods are provided for generating chondrocytes and cartilage tissue from human pluripotent stem cells. The methods include inducing chondrogenesis in monolayer cultures using one or more of FGF agonists, cAMP agonists, and TGFβ agonists. The articular cartilage tissue generated using the methods has a zonal organization similar to native cartilage tissue, including surface chondrocytes and midzone chondrocytes, with an increase in extracellular matrix components consistent with native cartilage tissue. These biochemical and mechanical properties make the cartilage tissue particularly suitable for tissue transplantation in vivo.

The application discloses a method for semi-quantitative analysis of expression level of secreted protein of human joint cartilage cells by using immunoblotting technology. The method comprises the following steps: 1) preparing a secreted protein culture solution; 2) collecting a sample of the secreted protein culture solution; 3) performing dialysis desalting treatment on the sample; 4) concentrating the secreted protein culture solution to prepare a powder; 5) re-dissolving the secreted protein culture solution powder and quantifying the protein; 6) separating the protein components in the re-dissolved culture solution; and 7) detecting the expression level of the secreted protein in the re-dissolved culture solution sample by using immunoblotting method. The low-temperature dialysis and vacuum concentration technology can significantly improve the concentration of the secreted protein in the human joint cartilage tissue culture solution sample under the condition of maintaining the integrity of the protein structure, and is suitable for detecting the chemical fluorescence signal of the secreted protein in the sample by using the immunoblotting technology. The application provides an important cell reaction detection tool for studying the pathogenesis of bone and joint diseases caused by degenerative changes of cartilage tissue and researching and developing biological small-molecule drugs for treating the diseases.

The invention provides a temporomandibular articular cartilage cell senescence model as well as a construction method and a detection method thereof, and belongs to the technical field of cell models. The invention provides a construction method of a temporomandibular articular cartilage senescence model. A low-dose topoisomerase II inhibitor is used as an inducer to induce temporomandibular articular cartilage cell senescence. By utilizing the method disclosed by the invention, typical senescence phenotypes can be obtained through induction only in 48-96 hours, the model construction period is short, and the efficiency is high; the induced cell model is more in line with the pathological process of chronic low-grade inflammation of temporomandibular joint disorder (TMD), and the phenotype is typical and stable; and the model difference between batches is small, the repeatability is good, and the cartilage characteristics are retained. The invention also improves the detection method of the temporomandibular articular cartilage cell senescence model, can directly judge whether the cell senescence is successfully induced or not through observation after the modeling is finished, has the characteristics of intuition and convenience, and is suitable for related experiments such as subsequent drug screening.

Disclosed herein are methods for treating osteoarthritis may be a one-step arthroscopic procedure and may include detaching synovial mesenchymal stem cells (MSCs) from the synovium using a brush device: covering articular cartilage in an affected joint with a scaffold, and placing concentrated MSC exosomes into the affected joint to stimulate differentiation of synovial MSCs into articular cartilage cells.

This invention discloses a *Taxillus chinensis* exosome-like nanovesicle and its application in the preparation of a therapeutic drug for temporomandibular joint osteoarthritis. The *Taxillus chinensis* exosome-like nanovesicle is prepared by differential ultracentrifugation combined with ultrafiltration. This invention demonstrates that *Taxillus chinensis* exosome-like nanovesicle can treat temporomandibular joint osteoarthritis through the following aspects: 1) Anti-inflammatory function: effectively reducing the expression of inflammatory factors. 2) Inhibition of cartilage degeneration function: blocking the degradation pathway of cartilage matrix and slowing down cartilage tissue damage. 3) Promotion of cartilage regeneration function: activating the synthetic activity of articular chondrocytes and enhancing cartilage repair capacity. 4) Restoration of condylar bone microstructure. The *Taxillus chinensis* exosome-like nanovesicle of this invention exhibits significant protective and repairing effects in alleviating bone and cartilage damage associated with temporomandibular joint osteoarthritis, and has promising application prospects.