16 results about "Articular chondrocyte" patented technology

The present application provides an activity analysis detection method for a stress-induced mesenchymal stem cell extract, comprising step one, culturing human primary articular chondrocytes; step two, aspirating the culture fluid, adding an equal volume of a composition to be tested, and incubating, wherein the composition to be tested includes a mesenchymal stem cell extract; step three, then adding an equal volume of a lipopolysaccharide solution with a concentration of 80ng / mL-120ng / mL, continuing to incubate, and collecting a cell lysate, which is used to detect inflammatory cytokines. The present application determines the optimal use concentration and cell incubation time of a mesenchymal stem cell extract (denoted as Alituo) and a lyophilized composition (denoted as Alip) and a lipopolysaccharide solution by measuring cell viability and the levels of inflammatory factors IL-6, IL-1β, and TNF-α. By detecting cell viability and inflammatory factors, the quality of the mesenchymal stem cell extract can be efficiently evaluated, and the activity analysis detection method has high repeatability and good stability.