40 results about "Articular chondrocyte" patented technology

The present invention provides defined serum-free cell culture media useful in culturing fibroblasts, especially articular chondrocytes, that avoids problems inherent in the use of serum-containing media. The defined media comprise platelet-derived growth factor (PDGF), and chemically defined lipids, or combinations of these compounds. In another aspect, the present invention also provides tissue culture methods that comprise incubating chondrocytes in the defined serum free media. The methods enhance attachment and proliferative expansion of chondrocytes seeded at low density while maintaining their redifferentiation potential.

This invention provides a new therapeutic or prophylactic agent for arthritis such as osteoarthritis. Specifically, it provides a therapeutic or prophylactic agent for arthritis such as osteoarthritis, or an agent for promoting the growth of articular chondrocyte, comprising a guanyl cyclase B (GC-B) activator as an active ingredient; or a method for inhibiting arthritis or for promoting the growth of articular chondrocyte by activating GC-B; or a method for screening an agent for promoting the growth of articular chondrocyte or an agent capable of treating arthritis using the GC-B activity as an indication.

Methods of promoting healing of a cartilage defect in a region of cartilage, which comprises the defect and which may further comprise stem cells, and methods of promoting healing of a cartilage defect in a region of cartilage, which comprises the defect and an implant comprising cartilage scaffold or a cartilage graft, which methods comprise contacting the region with various combinations of cartilage fragments, a growth factor, a partially synthesized extracellular matrix, a scaffold, an implant comprising cartilage scaffold, an implant comprising a cartilage graft, stem cells, chondrocytes, a proteoglycan, an anti-oxidant, a collagen precursor, a vitamin, a mineral, and/or a cartilage-degrading enzyme; and a method of causing stem cells to differentiate by the articular chondrocyte pathway comprising contacting the stem cells with a compound comprising an active alcohol moiety.

An agent for inhibiting articular cartilage extracellular matrix degradation of the present invention, comprising a histone deacetylase-inhibiting compound as an active ingredient, is effective for the prevention and treatment of diseases and pathological conditions involving the degradation and degeneration of the articular cartilage extracellular matrix, in particular, arthrosteitis, rheumatic arthritis, osteoarthritis, and the like.

The present invention provides defined serum-free cell culture media useful in culturing fibroblasts, especially articular chondrocytes, that avoid problems inherent in the use of serum-containing media. The defined media comprise platelet-derived growth factor (PDGF), chemically defined lipids, oncostatin M (OSM), interleukin-6 (IL-6), leukemia inhibitory factor (LIF), or combinations of these compounds. In another aspect, the present invention also provides tissue culture methods that comprise incubating chondrocytes in the defined serum-free media. The methods enhance attachment and proliferative expansion of chondrocytes seeded at low density while maintaining their redifferentiation potential.

Disclosed herein is a three-dimensional culture containing human articular chondrocytes with induced terminal differentiation changes, as well as a process for preparing the same. The three-dimensional culture, which was found to mimic the biological characteristics of articular chondrocytes that undergo terminal differentiation in vivo, can be used as a tool in the studies of the molecular and cellular mechanisms of osteoarthritis, and in the screening of candidate drugs for use in treatment of a disorder associated with articular chondrocytes.

The invention discloses a non-viral nanometer nucleic acid transportation compound used for treating cartilage defect through intra-articular injection which comprises low-molecular chitosan with molecular weight of 5KD-100KDand percentage composition of amino group of 30-70%, and nucleic acid, wherein N/P of the compound is 0.2:1-10:1.The invention degrades high molecular chitosan to low molecular chitosan, increases water solubility of material, reduces viscosity of solution, reduces influence of sour environment of solvent to cell, improves compound size with nanometer, is in favor of performing genetic transmission to cartilago articularis cell, and protects target nucleic acid from neutralizing with synovial fluid and being degrade by nuclease in lysosome. The invention has simple preparation and abroad application scope; can solve engineering roadblock of cartilage repair with no requirement of operation, complex device and bracket material.

The invention discloses application of FGF (fibroblast growth factor) 9 in preparation of osteoarthritis cartilage repair promotion medicines, researches find the FGF9 can play the role of retardation of abnormal differentiation of articular cartilage cells in the articular cartilage cells in the inflammatory environment, the FGF9 can alleviate the articular cartilage degradation in an osteoarthritis model in mice, can inhibit key enzyme MMP-13 of articular cartilage degradation, can slow down the hypertrophy process of articular cartilage, can be use for preparation of the osteoarthritis cartilage repair promotion medicines, and has good potential application prospect in the osteoarthritis treating field.

The invention provides cartilage preserving fluid and a utilization method thereof. The cartilage preserving fluid is prepared from monopotassium phosphate, histidine or histidine salt, lactobionic acid, saccharose, allopurinol, low-molecular dextran-40, NaCl, KCl, magnesium sulfate, penicillin, reduced glutathione, adenosine, trehalose, catechin, vitamin E and chondroitin sulfate, deionized waterserves as a solvent, a pH value is 7.35-7.52, and the cartilage preserving fluid is used for cartilage preservation at a temperature of 0-4 DEG C. The cartilage preserving fluid and the utilization method thereof have advantages that bioactivity of articular cartilage cells can be kept, degenerative changes of articular cartilages can be delayed, and assistances are provided for clinical treatment of large-area articular cartilage defects and cartilage tissue transplantation.

A method for culturing the cartilage cells of BALB/c mouse knee joint is disclosed. Its advantage is high surviral rate (80-90%).

The invention discloses an experimental method for researching anti-articular cartilage cell apoptosis of resveratrol. The ability of resveratrol to inhibit articular cartilage cell apoptosis is verified by establishing crowd research, in vivo experiments and in vitro experiments, and the mechanism of action of resveratrol to inhibit articular cartilage cell apoptosis is discussed to verify the 'effect axis hypothesis' of resveratrol-p38 MAPK signal pathway down-regulation-anti-apoptosis, which proves that the potential action targets of resveratrol in the screened p38 MAPK pathway have a targeting relationship, and further defines the overall regulation effect of resveratrol on a plurality of targets in the pathway. Experiments prove that resveratrol has the ability to inhibit articular cartilage cell apoptosis, and provides experimental basis for clinical practice of resveratrol in treating osteoarthritis.

It is intended to provide a method of three-dimensionally culturing normal joint chondrocytes; the production and supply of chondrocytes; and a transplantation material to be used in an injured site in a joint tissue.

The invention discloses a method for establishing an osteoarthritis osteoclast-like cell model of mesenchymal stem cells after directional cartilage differentiation through a two-step method. The method comprises the steps that firstly, the mesenchymal stem cells are induced to be subjected to directional differentiation into articular cartilage cells, and then the articular cartilage cells are induced by adopting IL-1beta in-vitro induction to establish the osteoarthritis model. According to the method, the two-step method is used for establishing the osteoarthritis model after directional cartilage differentiation for the first time, and by exploring the cartilage cell morphology and expression change of phenotype genes aggrecan and Co12alpha1, the optimal use concentration and cell induction time of a solution are determined. The method is easy to operate and low in cost and can be applied to related studies such as cell quality evaluation of the mesenchymal stem cells after directional cartilage differentiation, early warning of easy infection of adult osteoarthritis, verification of osteoarthritis drug action targets and screening of drug toxicity/drug functions.

A method for treating a mammal having damaged articular cartilage tissue includes harvesting a tissue sample from the mammal, growing articular cartilage cells from the tissue sample, and transplanting the articular cartilage cells into the damaged tissue. Growing the articular cartilage cells from the tissue sample can be accomplished by breaking the tissue sample into fragments, placing the fragments into a culture vessel, inducing at least some of the fragments to adhere to the culture vessel, and supplying the fragments with nutrients so that articular cartilage cells contained therein divide and grow.

The invention relates to a culture method for improving differentiation from peripheral blood multipotent cells to cartilage cells, and belongs to the technical field of cytobiology and regenerative medicine. The culture method comprises the following steps: carrying out primary culture on peripheral blood multipotent mesenchymal matrix cells to obtain third-generation cells; co-culturing the third-generation passed-down peripheral blood multipotent mesenchymal matrix cells and human articular chondrocytes in an induction culture medium. According to the culture method for improving the differentiation from the peripheral blood multipotent cells to the cartilage cells, the peripheral blood multipotent stem cells and the chondrocytes are co-cultured and the two types of cells mutually promote proliferation and the differentiation; the number of times of chondrocyte proliferation and passage and the quantity of the chondrocytes can be reduced, and the differentiation is improved; an efficient method is provided for establishing a seed cell source, and a new medical experiment evidence is also provided for enlarging a cell source of cartilage tissue engineering.

An in vivo assay to measure anchorage-independent growth and phenotypic stability of a certain cell population comprising subcutaneous or intramuscular injection in a mammal of a cell suspension of articular chondrocytes in an iso-osmotic liquid, the same suspension comprising articular chondrocytes in an amount equivalent to at least 1×10⁶ chondrocytes as applied to immune-deficient mice. The outcome is linked to molecular markers. The present invention further relates to DNA chips and diagnostic tools comprising the latter to predict the outcome of ACT. Antibodies raised against positive and negative markers of chondrocyte stability can also be used for quality control on the chondrocytes. Therapeutical composition comprising stable chondrocytes are very useful for tissue repair.

In general, this invention relates to compositions and methods useful in enhancing articular cartilage repair by providing for the sustained release of growth factors to articular cartilage cells to induce cell proliferation and extracellular matrix synthesis.

The invention relates to application of 2-aminoethoxy diphenyl borate in preparation of a medicine for treating osteoarthritis, and belongs to the technical field of biological medicines. According tothe invention, an apoptosis model is constructed on primary rat articular cartilage cells to simulate cartilage cell damage after osteoarthritis. The invention finds that 2-APB with different concentrations can obviously recover the cell viability and mitochondrial membrane potential level of apoptotic chondrocytes and reduce LDH leakage rate and ROS release, and can reduce the cartilage cell apoptosis by inhibiting IHH/Bcl-2/Bax signal transduction pathway, such that the related research on the cartilage cell protection mechanism is further supplemented and enriched, and the more scientificbasis is provided for the research and the development of new drugs for treating osteoarthritis.

The synthesis of collagen and proteoglycan in chondrocytes, such as intervertebral disc cells, articular chondrocytes and meniscal cells is promoted by administration of an extract from inflamed tissue inoculated with vaccinia virus.