Functionalized double-network hydrogel as well as preparation method and application thereof

Abstract

The invention relates to functionalized double-network hydrogel as well as a preparation method and application thereof. The functionalized double-network hydrogel is formed by interpenetrating a chitosan cross-linked network and a four-arm polyethylene glycol cross-linked network, wherein the chitosan cross-linked network is formed by chitosan ions coupled with KGN through cross-linking; the four-arm polyethylene glycol cross-linked network is formed by carrying out chemical reaction on a first precursor and a second precursor, wherein the first precursor is Tetra-PEG-NH2, and the second precursor is Tetra-PEG-NHS. The functionalized DN hydrogel provided by the invention not only has good mechanical properties and cell affinity, but also provides mechanical support for bone regeneration; and KGN can be slowly released, and necessary bioactive factors are continuously provided for seed cell osteogenic differentiation. The preparation method is simple in preparation process, low in price and easy to obtain, the double-network hydrogel can be prepared through simple steps, and no toxic initiator needs to be used.

Description

technical field [0001] The invention relates to the technical field of new medical materials, in particular to a functionalized double network hydrogel and its preparation method and application. Background technique [0002] Bone defects caused by inflammation, tumors, trauma, etc. are common and frequently-occurring diseases in humans, which seriously affect the physiological functions, physical and mental health and quality of life of patients. At present, the commonly used methods for repairing bone defects clinically include allograft bone, autologous bone transplantation, bone lengthening, etc., but the effect is often poor due to problems such as immune rejection, infectious diseases, and limited sources. Using tissue engineering technology, it is an effective treatment to use bone tissue engineering materials constructed from seed cells and biological scaffolds for bone defect repair. [0003] In tissue engineering, a good scaffold material is not only a cell carrie...

AI Technical Summary

Problems solved by technology

Although growth factors have a good induction effect on cells, problems such as short biological half-life, lack of long-acting effects, poor stability in vivo and poor tissue selection specificity have not been effectively resolved. These defects limit the clinical application of growth factors. Further applications in

[0005] The construction of traditional DN gels usually involves complex preparation steps, toxic initiators, and acrylamide starting materials

Meanwhile, most DN hydrogels are non-degradable, which may limit the infiltration of cells and the deposition and distribution of matrix, thereby hindering their application in cartilage tissue engineering.

[0006] In addition, as far as the current research is concerned, there is no research and product to modify KGN on the DN hydrogel scaffold material through a simple and efficient chemical synthesis method to promote the repair of large area bone defects.

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