Sustained-release high-efficiency poly-gamma-glutamic acid/dodecyl dimethyl benzyl ammonium chloride nano-bactericide as well as preparation method and application thereof

A technology of dodecyl dimethyl benzyl ammonium chloride and glutamic acid, which is applied in the medical field, can solve problems such as slow release, and achieve excellent biocompatibility, good slow release performance, and good antibacterial and inhibitory properties. The effect of bacteria

Inactive Publication Date: 2018-09-21
泰州先进化工有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Dodecyldimethylbenzyl ammonium chloride, also known as 1227, is a non-oxidizing fungicide with broad-spectrum and high-efficiency bactericidal and algae-killing capabilities. In the prior art, it is often used in combination with other fungicides. , but still can not solve the problem of its sustained release

Method used

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  • Sustained-release high-efficiency poly-gamma-glutamic acid/dodecyl dimethyl benzyl ammonium chloride nano-bactericide as well as preparation method and application thereof
  • Sustained-release high-efficiency poly-gamma-glutamic acid/dodecyl dimethyl benzyl ammonium chloride nano-bactericide as well as preparation method and application thereof
  • Sustained-release high-efficiency poly-gamma-glutamic acid/dodecyl dimethyl benzyl ammonium chloride nano-bactericide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of nano-antibacterial agent under the condition of poly-γ-glutamic acid / chitooligosaccharide mass ratio of 10:1

[0029] The preparation of step 1 novel high-efficiency nano antibacterial agent

[0030] 1) In the 8.75 mL system, prepare a poly-γ-glutamic acid / chitooligosaccharide solution with a mass ratio of 10:1 (total concentration: 5 mg / mL) (the solvent is deionized water, the same below), and stir evenly. Prepared into poly-γ-glutamic acid / chitooligosaccharide matrix solution;

[0031] 2) EDC and NHS were prepared into 0.2 M concentration solutions respectively, and mixed at a ratio of 2:1 to obtain an EDC / NHS solution. Take 1.25 mL of the prepared EDC / NHS solution and add it to the prepared matrix solution, and stir at room temperature for 5 h, after forming a homogeneous solution, ultrasonically crush for 5 h to prepare loaded nanoparticles;

[0032] 3) Prepare 10 mL of 1227 solution with a concentration of 400 μg / mL, add it to the prepared nanopart...

Embodiment 2

[0043] Example 2 Preparation of nano-antibacterial agent under the condition of 12:1 mass ratio of poly-γ-glutamic acid / oligochitosan

[0044] The preparation of step 1 novel high-efficiency nano antibacterial agent

[0045] 1) In an 8.75 mL system, prepare a poly-γ-glutamic acid / chitooligosaccharide solution with a mass ratio of 12:1 (total concentration: 5 mg / mL), stir evenly, and prepare a poly-γ-glutamic acid / shell Oligosaccharide matrix solution;

[0046] 2) EDC and NHS were prepared into 0.2 M concentration solutions respectively, and mixed at a ratio of 2:1 to obtain an EDC / NHS solution. Take 1.25 mL of the prepared cross-linking agent EDC / NHS solution and add it to the prepared matrix solution. , stirred for 5 h to form a homogeneous solution, and ultrasonically crushed for 5 h to prepare loaded nanoparticles;

[0047] 3) Prepare 10 mL of 1227 solution with a concentration of 400 μg / mL, add it to the prepared nanoparticle solution at a ratio of 1:1, and continue to s...

Embodiment 3

[0058] Example 3 Preparation of nano-antibacterial agent under the condition that the mass ratio of poly-γ-glutamic acid / oligochitosaccharide is 14:1

[0059] The preparation of step 1 novel high-efficiency nano antibacterial agent

[0060] 1) In an 8.75 mL system, prepare a poly-γ-glutamic acid / chitooligosaccharide solution with a mass ratio of 14:1 (total concentration: 5 mg / mL), stir evenly, and prepare a poly-γ-glutamic acid / shell Oligosaccharide matrix solution;

[0061] 2) EDC and NHS were prepared into 0.2 M concentration solutions respectively, and mixed at a ratio of 2:1 to obtain an EDC / NHS solution. Take 1.25 mL of the prepared cross-linking agent EDC / NHS solution and add it to the prepared matrix solution. , stirred for 5 h to form a homogeneous solution, and ultrasonically crushed for 5 h to prepare loaded nanoparticles;

[0062] 3) Prepare 10 mL of 1227 solution with a concentration of 400 μg / mL, add it to the prepared nanoparticle solution at a ratio of 1:1, a...

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Abstract

The invention belongs to the technical field of medicines and relates to a sustained-release high-efficiency poly-gamma-glutamic acid/dodecyl dimethyl benzyl ammonium chloride nano-bactericide as wellas a preparation method and application thereof. The bactericide is prepared from poly-gamma-glutamic acid and dodecyl dimethyl benzyl ammonium chloride. The prepared bactericide has excellent bacteria resisting and inhibiting effects, has excellent sustained-release property and uniform particle size, and can reach a 1227 load rate of 34.2-47.8%. The nano-bactericide prepared in the invention has multiple excellent properties of being non-toxic, biodegradable, pollution-free, high in biocompatibility and the like, and has wide application prospects.

Description

technical field [0001] The invention belongs to the field of medical technology, and relates to a sustained-release high-efficiency poly-γ-glutamic acid / dodecyldimethylbenzyl ammonium chloride nano-bactericide, a preparation method and an application thereof. Background technique [0002] With the arrival of summer, the weather gets warmer, the temperature rises, and the reproduction of many bacteria and fungi gradually increases. How to protect public health and deal with public health places, food industry preparation places, medical equipment, and laboratory personnel often use Sterilization of various instruments, inhibiting the reproduction of harmful bacteria and fungi, and avoiding epidemic diseases have become the primary issues that people are increasingly concerned about. Experimental and medical personnel often use chemicals such as ethylene oxide to sterilize experimental equipment, but the waste liquid is difficult to handle, causing a wider range of pollution a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N33/12A01N25/10A01P1/00A01P3/00A61K31/14A61P31/04B82Y30/00B82Y40/00A61K9/51A61K47/34A61K47/36A61P17/02
CPCA01N25/10A01N33/12A61K9/5146A61K9/5161A61K31/14A61P17/02A61P31/04B82Y30/00B82Y40/00
Inventor 杨革梁鑫鑫车程川
Owner 泰州先进化工有限公司
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