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Heterojunction photo-thermal agent and preparing method and application thereof

A photothermal reagent and heterojunction technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, bone diseases, etc., can solve the problems of limited PTT application, high laser irradiation power density, low photothermal conversion efficiency, etc. To achieve the effect of good induction of osteogenic differentiation of mesenchymal stem cells, simple preparation process and high biocompatibility

Inactive Publication Date: 2019-10-25
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, their rather low photothermal conversion efficiency requires high laser irradiation power density to complete tumor ablation, which limits their PTT applications.

Method used

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  • Heterojunction photo-thermal agent and preparing method and application thereof
  • Heterojunction photo-thermal agent and preparing method and application thereof
  • Heterojunction photo-thermal agent and preparing method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0048] A method for preparing a heterojunction photothermal reagent, specifically comprising the following steps:

[0049] (1) The method for preparing tungsten sulfide nanosheets:

[0050] S11. Take 200mg of tungsten sulfide powder and place it in a grinder for grinding for 2 hours at a speed of 400r / min, then take 40mg of ball-milled tungsten sulfide powder and disperse it in 40mL of sulfuric acid, and react at 90°C for 24 hours;

[0051] S12, the prepared product is collected by centrifugation at 8000r / min and washed several times with deionized water to remove residual H 2 SO 4 , and finally disperse the product collected by centrifugation into 40mL deionized water and sonicate for 2h;

[0052] S13. Finally, centrifuge the dispersion at 8000r / min to collect the tungsten sulfide nanosheets, disperse them in 20mL deionized water, and set aside;

[0053] (2) Preparation of graphene quantum dots responding in the second near-infrared region:

[0054] S21. Add 0.025mg of 1,...

Embodiment 2

[0062] (1) In vitro CT imaging of graphene quantum dots / tungsten sulfide heterojunction:

[0063] For in vitro CT imaging, the graphene quantum dots / tungsten sulfide heterojunction was diluted to different concentrations (0, 0.625, 1.25, 2.5, 5, 10 mg mL -1 ) into a 0.5mL centrifuge tube for CT imaging ( Figure 6 a).

[0064] (2) In vivo CT imaging of graphene quantum dots / tungsten sulfide heterojunction:

[0065] For in vivo CT imaging, establish an osteosarcoma (MG-63) tumor-bearing mouse model until the tumor volume reaches 500mm 3 When left or right, inject 200 μL of 1.0 mg mL intravenously into mice -1 The graphene quantum dot / tungsten sulfide heterojunction dispersion liquid was taken at 0h and 24h respectively to observe the CT imaging effect of the mouse tumor site ( Figure 6 c).

Embodiment 3

[0067] (1) In vitro photothermal properties of graphene quantum dots / tungsten sulfide heterojunction:

[0068] Dilute the graphene quantum dots / tungsten sulfide heterojunction to different concentrations (0, 0.3, 0.4, 0.5, 0.6mg mL -1 ), then transfer 1.0mL of the solution to a 1.5mL centrifuge tube, and use 808nm and 1064nm lasers at 0.2W cm -2 and 0.6W cm -2 The power density was irradiated for 5 minutes, and deionized water was used as a control group, and the temperature change was recorded with an infrared thermal imager every 30s ( Figure 4 ).

[0069] (2) In vivo photothermal therapy performance evaluation of graphene quantum dots / tungsten sulfide heterojunction:

[0070] Subcutaneously implant 100 μL (1 million) osteosarcoma cells (MG-63) in the armpits of 3-5-week-old female nude mice until the tumor volume grows to 100 mm 3 , the nude mice were divided into 5 groups (5 in each group): (1) normal saline, (2) graphene quantum dots / tungsten sulfide heterojunction, ...

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Abstract

The invention discloses a novel photo-thermal chemotherapy combined treatment agent and a preparing method and application thereof. The preparing method includes the steps of firstly, preparing tungsten sulfide nanosheets; secondly, preparing graphene quantum dots responded to by a near infrared second region; thirdly, severely stirring the tungsten sulfide nanosheets with the graphene quantum dots responded to by the near infrared second region in a phosphate buffer salt solution with the pH of 8.0, and conducting centrifuging treatment on the mixed liquid to remove unbound graphene quantum dots to obtain the heterojunction photo-thermal agent with the graphene quantum dots responded to by the near infrared second region and the tungsten sulfide. The prepared heterojunction photo-thermalagent has a good osteosarcoma treatment effect and a mesenchymal stem cell osteogenic differentiation effect; the nanometer photo-thermal agent is high in biocompatibility, free of toxin and pollution, simple in preparing process, low in cost and suitable for large-scale production and clinical medical use.

Description

technical field [0001] The invention relates to the technical field of nano-medicines, in particular to a heterojunction photothermal reagent and its preparation method and application. Background technique [0002] With the advancement of science and technology, many incurable diseases have been effectively curbed, but cancer is still a worldwide problem that is difficult to overcome. Osteosarcoma is a malignant tumor derived from bone tissue. Although the existing drugs for the treatment of malignant tumors have made great progress in the past few decades, traditional treatment methods such as surgery, radiotherapy and chemotherapy usually lead to Frequent recurrence of malignancy, metastasis of tumor cells and destruction of the immune system. Therefore, a large number of researchers are devoting themselves to the exploration and development of low-toxicity and high-efficiency therapeutic strategies. Among them, photothermal therapy (PTT) is a major therapeutic approach...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61P35/00A61P19/08
CPCA61K41/0052A61P19/08A61P35/00
Inventor 沈龙祥耿弼江潘登余
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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