Preparation method of a double-layer composite scaffold for inducing pulp dentin tissue regeneration

Abstract

The invention relates to a method for preparing a double-layer composite scaffold for inducing pulp dentin tissue regeneration, comprising the following steps: (1) preparation of bioactive glass (PSC) synthesized by taking phytic acid as a precursor; (2) polypeptide of PSC Modification; (3) preparation of the outer part of the stent; (4) preparation of the inner part of the stent; (5) completion of cross-linking of the double-layer stent: soak the stent obtained in step (4) in 0.1%-0.5% In the glutaraldehyde cross-linking agent, the inner and outer layer scaffolds are cross-linked to form a composite scaffold with a double-layer structure. The invention has layering inducing ability and conforms to the root canal shape of the tooth, and is a bioactive scaffold material for inducing the regeneration of the pulp-dentine composite tissue; the inner layer of the scaffold can migrate and crawl in chemotactic stem cells, which is beneficial to the generation of pulp-like soft tissue ; The outer layer of the scaffold can further induce the odontogenic differentiation of cells and promote the generation of dentin-like hard tissue; finally form a special soft and hard tissue similar to natural teeth.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a method for preparing a double-layer composite support for inducing pulp dentin tissue regeneration. Background technique [0002] Human teeth are susceptible to caries, trauma, or occlusal trauma, which can cause damage to the pulp and dentin. Pulp damage is difficult to reverse and is prone to necrosis. At present, the main method of clinical treatment is root canal therapy, which is to use inert material to tightly fill the root canal after debridement. Restricted by the complex anatomical structure of the root canal system, root canal treatment techniques are complex and cost a lot of social medical costs; mechanical and chemical drug debridement is required, which is potentially harmful to the periapical tissue. The important thing is that after losing the pulp, the tooth cannot continue to form secondary dentin and tertiary dentin, and loses the ability to se...

AI Technical Summary

Problems solved by technology

However, the effect of the above-mentioned materials is single, and it is impossible to achieve the purpose of layering the soft and hard tissues of the pulp and dentin.

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