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Active microspheres capable of directionally regulating and controlling chondrocyte accumulation and preparation method of active microspheres

A microsphere, bioactive technology, applied in the direction of bone/connective tissue cells, animal cells, vertebrate cells, etc., can solve the problem of lack of active regulatory cells and so on

Inactive Publication Date: 2012-09-26
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cells tend to concentrate and accumulate locally in traditional three-dimensional porous scaffolds, but none of these scaffolds have the ability to actively regulate cell accumulation

Method used

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  • Active microspheres capable of directionally regulating and controlling chondrocyte accumulation and preparation method of active microspheres
  • Active microspheres capable of directionally regulating and controlling chondrocyte accumulation and preparation method of active microspheres

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Experimental program
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Effect test

Embodiment 1

[0016] The preparation of embodiment 1 active porous microspheres:

[0017] (1) Preparation of porous polylactic acid polymer microspheres: 1.25ml of 5% NH 4 HCO 3 Solution (W 1 phase) was added to 4ml of polylactic acid polymer organic solution with a concentration of 3.13% (O phase), and then stirred at a high speed with a homogeneous mixer in an ice bath to obtain the primary emulsion (W 1 / O), quickly pour the resulting primary emulsion into 400ml 0.1% PVA aqueous solution (W 2 phase), mechanically stirred at room temperature to obtain a secondary emulsion (W 1 / O / W 2 ), the organic solvent volatilizes in the secondary emulsification process and finally obtains porous polylactic acid polymer microspheres, which are repeatedly washed with deionized water, and finally freeze-dried to obtain the desired microspheres; (2) chemical crosslinking: the porous polylactic acid polymer microspheres Soak the ball in 50ml ethanol / water (1 / 1, v / v) mixed solvent for 2-3 hours to rem...

Embodiment 2

[0018] The preparation of embodiment 2 active porous microspheres:

[0019] (1) Preparation of porous polylactic acid polymer microspheres: 1.25ml of 5% NH 4 HCO 3 Solution (W 1 phase) was added to 4ml of polylactic acid polymer organic solution with a concentration of 3.13% (O phase), and then stirred at a high speed with a homogeneous mixer in an ice bath to obtain the primary emulsion (W 1 / O), quickly pour the obtained primary emulsion into 400ml of 0.1% PVA aqueous solution (W 2 phase), mechanically stirred at room temperature to obtain a secondary emulsion (W 1 / O / W 2), the organic solvent volatilizes in the secondary emulsification process and finally obtains porous polylactic acid polymer microspheres, which are repeatedly washed with deionized water, and finally freeze-dried to obtain the desired microspheres; (2) chemical crosslinking: the porous polylactic acid polymer microspheres Soak the ball in 50ml ethanol / water (1 / 1, v / v) mixed solvent for 2-3 hours to re...

Embodiment 3

[0020] The preparation of embodiment 3 active porous microspheres:

[0021] (1) Preparation of porous polylactic acid polymer microspheres: 1.25ml of 5% NH 4 HCO 3 Solution (W 1 phase) was added to 4ml of polylactic acid polymer organic solution with a concentration of 3.13% (O phase), and then stirred at a high speed with a homogeneous mixer in an ice bath to obtain the primary emulsion (W 1 / O), quickly pour the resulting primary emulsion into 400ml 0.1% PVA aqueous solution (W 2 phase), mechanically stirred at room temperature to obtain a secondary emulsion (W 1 / O / W 2 ), the organic solvent volatilizes in the secondary emulsification process and finally obtains porous polylactic acid polymer microspheres, which are repeatedly washed with deionized water, and finally freeze-dried to obtain the desired microspheres; (2) chemical crosslinking: the porous polylactic acid polymer microspheres Soak the ball in 50ml ethanol / water (1 / 1, v / v) mixed solvent for 2-3 hours to rem...

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Abstract

The invention provides a porous microsphere bracket material capable of regulating and controlling the directionally-accumulation biological activity of chondrocytes and a preparation method of the porous microsphere bracket material. The preparation method can be applied to preparation of substitutes for chondrocyte accumulation in a embryonic development process and in a clinical cartilage defect recovery process, and can be applied to the construction of biological active bracket materials in the fields of cartilage tissue recovery and the like. According to the method, active porous microspheres are prepared in the following steps of preparation of porous polyester high-molecule microspheres, chemical crosslinking and drying. The microspheres obtained by the method are high in biocompatibility and degradability and rational in physicochemical property and in microscopic topology morphology, and have porous structures with excellent connectivity and biological activity factor concentration gradient from inside to outside, thus being capable of regulating and controlling the directional chondrocyte accumulation in vitro, providing a good carrier for the in-vitro function expression of the chondrocytes, and serving as a good biological bracket material for tissue engineering cartilage.

Description

technical field [0001] The invention relates to the accumulation of chondrocytes during embryonic development, a substitute in the process of cartilage defect repair in clinic and a bioactive scaffold material for constructing cartilage tissue repair, specifically a bioactive porous microsphere capable of regulating the directional accumulation of chondrocytes Preparation method of scaffold material. Background technique [0002] Not only is cell accumulation crucial in the embryonic development of cartilage, but the timing, space, and manner in which accumulation occurs can significantly affect the maintenance and regeneration of adult cartilage. During embryonic development, chondrocyte precursor cells accumulate to form the contours of early embryonic cartilage limb buds; subsequently, cell accumulation resulting from degradation of the extracellular matrix by hyaluronidase triggers further chondrocyte differentiation and tissue formation. In adult cartilage, chondrocyte...

Claims

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Application Information

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IPC IPC(8): C08J9/42C08J9/40C08J9/28C08J3/24C08L67/00C08L67/04A61L27/18A61L27/54A61L27/56C12N5/077
Inventor 葛子钢李超
Owner PEKING UNIV
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