9results about How to "Improve functional activity" patented technology

The invention discloses a method for immobilizing growth-promoting rhizobacteria by taking decarburized gangue-based biochar as a carrier, a solid waste-based microorganism immobilization ball prepared by the method and application of the solid waste-based microorganism immobilization ball, and relates to the technical field of solid waste utilization. The method comprises the following steps: preparing gangue-based zeolite by microwave activation and decarburization of coal gangue; preparing gangue-based biochar by utilizing phosphorus modification; and preparing the solid-waste-based microorganism solidification balls by utilizing an adsorption-embedding-crosslinking method. The decarburized gangue-based biochar is used as a carrier material for immobilizing the growth-promoting rhizosphere bacteria, and is used for restoring the sandy degraded soil in the northwest arid mining area. According to the technology, the survival time and functional activity of the growth-promoting rhizobacteria in the soil environment can be improved, and a new way is provided for consumption of bulk gangue solid waste. The method also has the technical advantages of improving the carbon source utilization rate of the gangue and increasing the phosphate fertilizer and the organic carbon library of the soil.

The invention discloses a phycocyanin bacterium enzyme synergistic treatment product with high functional activity and a preparation method thereof, and belongs to the technical field of health food processing. The method comprises the following steps: firstly, carrying out enzymolysis on phycocyanin by using flavourzyme to obtain a phycocyanin enzymolysis product, preparing the enzymolysis product into a solution, inoculating lactobacillus plantarum into the solution for fermentation, and freeze-drying fermentation liquor to obtain the phycocyanin enzymolysis fermentation product subjected to bacterium-enzyme synergistic treatment. The method is simple in preparation process and short in overall time consumption, and the obtained product has antioxidant activity and anti-inflammatory activity superior to those of phycocyanin, corresponding phycocyanin fermentation products and phycocyanin enzymatic hydrolysate, has good flavor and color, and has good market application prospects. The invention not only provides a new product with high functional activity, but also provides a new thought and choice for expanded application of phycocyanin.

The present application belongs to the technical field of food processing, and specifically relates to a physical preparation method of high-resistance starch ultra-micro powder. The high-resistance starch rice with a dry basis resistance starch content of greater than or equal to 30% is used as raw material, and a pure physical processing route is adopted. The rice is first made crisp through twice low-temperature baking at 100-120 DEG C, and the structure of the resistance starch is stabilized at the same time. Then, after coarse crushing, the rice is sent into an airflow pulverizer, and wind-driven ultra-micro grinding is carried out in a low-temperature environment. Finally, the ultra-micro powder is obtained through grading. In the present application, no chemical reagent and enzyme preparation is used in the whole process, the processing temperature is lower than the thermal decomposition temperature of the resistance starch, the retention rate of the finished product resistance starch is greater than or equal to 93%, the dry basis content is greater than or equal to 28%, the powder is fine and has good cold water dispersibility, and the shelf life at room temperature can be more than 12 months. The present application has the advantages of simple process, controllable cost, suitability for large-scale production, no need for customizing special devices, short process flow, no complex reaction, separation and purification processes, low equipment investment and operation cost, and easy realization of large-scale industrial production.

The present invention relates to the field of pharmaceutical biotechnology, and specifically to the use of laminarin for antiviral and auxiliary sterilization; a preparation method of laminarin and its application in preparing antiviral sterilization liquid are disclosed, and the preparation method is: take dried kelp, crush it, add it into water, add hydrolase to fully enzymolyze it, inactivate the enzyme after enzymolysis, centrifuge and take the supernatant for freeze-drying to obtain laminarin. Through experimental comparison, it is found that the laminarin prepared by papain is particularly effective in resisting influenza virus. The laminarin antiviral sterilization liquid of the present invention has laminarin as an active ingredient, has significant anti-influenza virus effect, especially influenza A virus, and has certain antibacterial effect, and can be used as a disinfection product or medicine with significant inhibition of influenza A virus and auxiliary inhibition of bacteria and fungi.

This invention belongs to the field of plant tissue culture technology, specifically disclosing a method for producing Polygonatum polysaccharides using Polygonatum yunnanense tissue culture, comprising the following steps: explant disinfection, callus induction, callus proliferation and induction culture: selecting vigorous callus tissue, transferring it to a culture medium: White medium + 2,4-D 1.5 mg / L + VC 20 mg / L + sucrose 40 g / L + 30 mg / L inducer + agar 6 g / L, pH 5.8, after culture, collecting the callus, drying it, and then extracting Polygonatum yunnanense polysaccharides; the inducer is Polygonatum yunnanense endophytic basidiomycetes (… Talaromyces sp. The fermentation broth extract of HJF23; the HJF23 preservation number is CCTCC NO: M2021344. This invention not only realizes the efficient biosynthesis of Polygonatum yunnanense polysaccharide, but also significantly enhances its functional activity.

The invention relates to the technical field of yellow rice wine, in particular to coix seed red yeast rice yellow rice wine with high antioxidant activity as well as a preparation method and application thereof. The monascus ruber (3.15746) is used for carrying out microbial fermentation conversion on the coix seeds, and the coix seed and monascus ruber yellow rice wine is short in production period, excellent in sensory quality, rich in lovastatin and high in antioxidant activity. The monascus yellow wine strain is enriched, the content of active ingredients and the health-care effect of the monascus yellow wine are improved, and a feasible scheme and technical parameters can be provided for industrial development.

The present invention discloses a preparation method of cod skin active polypeptide powder and its use for antiviral and sterilization. Clean cod skin is soaked in water to swell, then water is added for homogenization, and then protease is added for hydrolysis. After the hydrolysis reaction is completed, the enzyme is inactivated and centrifuged, and the supernatant is freeze-dried to obtain the cod skin active polypeptide powder. The spray prepared by using the cod skin active polypeptide powder can effectively inhibit pathogenic bacteria, has a significant inhibitory effect on influenza A virus, and can be used as an antiviral, sterilization health product, medicine or disinfectant product. The present invention enriches the product form of cod skin by making the prepared cod skin polypeptide powder into a spray, is applicable to a wide range of people, avoids the waste of cod skin resources, realizes the high-value utilization of cod, and is of great significance for the development and utilization of cod skin products.

The present invention discloses a high-fidelity LnCBE system for realizing efficient C / G to T / A editing on the gC motif of DNA. The editing system constructs a backbone vector by engineering the segmentation protein of the DddA domain of the double-stranded DNA cytosine deaminase from Lachnospiraceae bacterium sunii NSJ‑8, which can realize more efficient on-target editing and extremely low off-target editing on DNA compared with the original version of the deaminase. At the same time, a DNA double-stranded deaminase immunosuppressive protein is disclosed, which can effectively inhibit the off-target editing of nuclear DNA in the LnCBE editing system. The LnCBE editing system can be used to prepare a gene editing kit, construct an animal model of mitochondrial DNA pathogenic mutation from C / G to T / A, and provide a new tool for gene editing technology.