177results about How to "Improve functional activity" patented technology

A red blood cell storage composition includes a composition of red blood cells and biochemistry altering reagents, the biochemistry altering reagents being present at a concentration so as to reduce the percent hemolysis of the red blood cells during the freeze-thaw cycle below that of the percent hemolysis of the red blood cells in the absence the biochemistry altering reagents. The red blood cell storage composition preferably includes reagents selected from: modifiers of glycolytic/metabolic components, modifiers of antioxidant potential, effectors of intracellular ionic distribution, modifiers of membrane fluidity, modifiers of cytoskeletal structure, effectors of the cyclooxygenase second messenger pathway, effectors of the lipoxygenase second messenger pathway, effectors of the hexose monophosphate second messenger pathway, effectors of the phosphorylation second messenger pathway, modifiers of specific messenger molecules, and combinations thereof.

The invention belongs to the technical field of biology, and discloses a polypeptide containing two chimeric antigen receptor structures, nucleic acid for coding the polypeptide, a modified T lymphocyte, and a preparation method and application of the T lymphocyte. The polypeptide provided by the invention contains the two chimeric antigen receptor (CAR) structures, wherein one chimeric antigen receptor structure specifically targets a tumor cell, and the other one specifically targets a normal B lymphocyte, so that a CAR-T cell is effectively amplified through recognizing the B lymphocyte ofa blood circulation system, and more CAR-T cells arrive at a solid tumor part so as to specifically kill the tumor cell for improving a treatment effect on the solid tumor. The polypeptide further comprises an ScFv structure of an anti-PD-L1 monoclonal antibody, and the CAR-T cells can simultaneously secrete a PD-L1 monoclonal antibody which interdicts an immunosuppressive action medicated by a PD1/PDL1 signal pathway and T cell failure, so that the treatment effect on the solid tumor is improved, the functional activity of immune cells in vivo is prolonged at the same time, and finally the tumor recurrence is retarded and even avoided.

The invention relates to functional peanut small molecule mixed polypeptide, a preparation method and application thereof, belonging to small molecule mixed polypeptide generated by peptide chain hydrolysis.The invention is characterized in that the functional peanut small molecule mixed polypeptide is prepared by adopting the following raw material components by weight part: 100 parts of defatted peanut cake, 6-10 parts of combined enzyme preparations, 6-12 parts of active carbon, 3-8 parts of diatomite, 3-8 parts of pearlite and 100 parts of water; and the following technical indexes are achieved: the molecular weight distribution range Dalton (D) is less than or equal to 3000, the content of the small molecule mixed polypeptide is more than or equal to 80wt, the total nitrogen is more than or equal to 12wt%, the water solubility is 90wt%, the water is less than or equal to 5wt%, and the fat is less than or equal to 0.5wt%.The invention provides a product of the functional peanut small molecule mixed polypeptide with high purity, good solubility, average molecular weight of less than 3000D and strong biological functionality and a preparation method of the functional peanut small molecule mixed polypeptide with short production cycle, high product yield and low preparation cost. The functional peanut small molecule mixed polypeptide is suitable for a raw material of medicines, health food and beverage and functional nutritious food.

The invention provides methods and compositions for promoting neural cell growth and/or regeneration. The general methods involve contacting with an activator of a cyclic nucleotide dependent protein kinase a neural cell subject to growth repulsion mediated by a neural cell growth repulsion factor. The activator may comprise a direct or an indirect activator of the protein kinase; the repulsion factor typically comprises one or more natural, endogenous proteins mediating localized repulsion or inhibition of neural cell growth; and the target cells are generally vertebrate neurons, typically injured mammalian neurons. The subject compositions include mixtures comprising a neural cell, an activator of a cyclic nucleotide dependent protein kinase and a neural cell growth repulsion factor.

The present invention discloses a novel black garlic-hawthorn beverage and a processing method thereof, and belongs to the field of food processing. The black garlic-hawthorn beverage comprises, 2-5 parts of black garlic, 26-30 parts of a hawthorn juice, 5-7 parts of honey, and 60-65 parts of purified water. The processing method comprises: (1) preparing black garlic, wherein garlic selecting and peeling, garlic clove crushing, temperature control vacuum fermentation, and dewatering drying are performed; (2) preparing a hawthorn juice; and (3) blending, homogenizing, sterilizing, and filling. According to the present invention, the method is simple, efficient, green and health, the influence of the processing process on the black garlic SOD activity is reduced, the product function activity is improved, and the product of the present invention has characteristics of sour, sweet and mild taste and rich nutrition, can meet the taste requirements of consumers, and further has good health effect.

The invention discloses mulberry leaf green juice powder and a preparation method of a mulberry leaf green juice solid beverage. The method comprises the steps of screening, cleaning, scalding, drying and low-temperature airflow crushing. The mulberry leaf green juice powder can fully utilize the nutrient substances contained in the mulberry leaves, is convenient to eat, and can be widely applied to the fields of food and natural pigments. The invention also provides a preparation method of the mulberry leaf green juice solid beverage. By taking the mulberry leaf green juice powder having the functions of reducing blood sugar, reducing blood pressure and reducing blood fat as a main raw material, the mulberry leaf green juice solid beverage plays an extremely high function through the mutual effect of the mulberry leaf green juice powder with green tea powder, barley seedling green juice powder, kelp powder, cyclic oligosaccharide, lactose, vitamin C, etc. on the basis of the original functions of the mulberry leaves, and is easy to drink.

The invention belongs to the technical field of formulas with homology of medicine and food and new resource foodstuff, and concretely provides medical total-nutrient formula food, specific medical formula food or non-specific medical formula food which is edible by pregnant-woman diabetes patients. The formula is prepared according to according to requirements of General Rule for Food for Special Medical Purpose by combining with physique characteristic of pregnant-woman diabetes patients. According to the traditional Chinese medicine quintessence theory, multiple medicinal and edible traditional Chinese medicines are subjected to reasonable compatibility, and medicinal and edible traditional Chinese medicine extractive essences extracted by employing a semi-bionic extraction process, multiple microencapsulated probiotics, oligopeptide extracted through biological enzymolysis, prebiotic, amino acids, carbohydrates, grease with health-care function, multiple vitamins and minerals are mixed and are subjected to other processing, so that the food is prepared. The food can be employed as a single nutrition source to satisfy nutrition demand of hepatosis patients, and also gives play to efficacies of enhancing insulin activity, reducing blood sugar, reducing blood fat, reducing fat and losing weight, and enhancing immunity.

The invention provides a porous microsphere bracket material capable of regulating and controlling the directionally-accumulation biological activity of chondrocytes and a preparation method of the porous microsphere bracket material. The preparation method can be applied to preparation of substitutes for chondrocyte accumulation in a embryonic development process and in a clinical cartilage defect recovery process, and can be applied to the construction of biological active bracket materials in the fields of cartilage tissue recovery and the like. According to the method, active porous microspheres are prepared in the following steps of preparation of porous polyester high-molecule microspheres, chemical crosslinking and drying. The microspheres obtained by the method are high in biocompatibility and degradability and rational in physicochemical property and in microscopic topology morphology, and have porous structures with excellent connectivity and biological activity factor concentration gradient from inside to outside, thus being capable of regulating and controlling the directional chondrocyte accumulation in vitro, providing a good carrier for the in-vitro function expression of the chondrocytes, and serving as a good biological bracket material for tissue engineering cartilage.

A protein produced by gene recombinant technology, including at least one domain selected from domains I, II, and III of serum albumin but having a different structure from that of native albumin; and a method of producing the protein. The protein has an enhanced functional activity or activities selected from among various functional activities or serum albumin including antibacterial activity, antioxidative effect, inflammation inhibitory effect, in vivo substance transporting action, and enzymatic activity.

The invention relates to a method for processing edible lobster sauce, , in particular to a method for processing bacteria-type lobster sauce using edible soy through steps of germinating, fermenting, sugaring, soy saucing, and the like. The invention solves the problems that the prior soy processing method can not release the nutrient elements of soy maximally and can not remove inhibitory factors for affecting the release of the nutrient elements and absorption of man body in soy maximally. The method comprises the main steps as follows: choosing edible soy, cleaning, steeping and germinating; stewing for sterilization; fermenting; sugaring, or soy saucing; and packaging. The invention has the advantage that the lobster sauce produced by the method has characteristics of low salt content, rich nutrient substance, high activity and proper taste, and is easy for digestive absorption.

The invention discloses a suaeda glauca herb health beverage with ageing delaying effect. The suaeda glauca herb health beverage is prepared by scientifically matching suaeda glauca herb, enteromorpha prolifera and Chinese herbal medicine raw materials; the formula preferably comprises 20 to 30 parts of the suaeda glauca herb, 20 to 30 parts of the enteromorpha prolifera, 5 to 10 parts of fructus lycii, 5 to 10 parts of poria cocos, 2 to 5 parts of radix ginsengs, 10 to 15 parts of radix acanthopanacis senticosi, 3 to 5 parts of rhizoma atractylodis macrocephalae, 3 to 5 parts of liquorice roots, 2 to 3 parts of Chinese galls, 3 to 5 parts of medicated leaven and 3 to 5 parts of folium steviae rebaudianae; and the suaeda glauca herb health beverage is prepared according to a process of carrying out enzymolysis, fermenting, leaching and extracting, mixing, blending, filling and sterilizing. The product has the advantages that the suaeda glauca herb, the enteromorpha prolifera and the Chinese herbal medicines have a synergistic effect so that the product has remarkable effects of resisting ageing, tonifying qi and benefiting, strengthening and consolidating body resistance, resisting fatigue, enhancing the immunity, prolonging life and the like, is safe and has no side effect, is convenient to eat and has a relatively good market application prospect.

The invention provides an application of pearl oyster small molecule peptide in skin wound repair. The pearl oyster small molecule peptide has a new application of repairing skin wounds. The preparation method of the pearl oyster small molecule peptide comprises the following steps: S1, preparing a pearl oyster zymolyte; and S2, selecting a ceramic membrane with a pore diameter of 0.05-0.5 mu m, performing full filtration, separating the material by adopting ultrafiltration membranes with different molecular weight cutoff, collecting peptide fragments with different molecular weights, including any one of bioactive peptide with the molecular weight of less than 3000Da, bioactive peptide with the molecular weight of 3000Da-5000Da and bioactive peptide with the molecular weight of 5000Da-10000Da, and performing freeze-drying to obtain the pearl oyster small molecule peptide. The invention provides a new application of the pearl oyster small molecule peptide in preparing a medicine for repairing skin wounds such as skin burn, wound, burn and scald, the effect of the pearl oyster small molecule peptide is obviously superior to that of the medicine in the prior art, the wound is quicklyrepaired, no scar is left, and the effect of promoting wound repair is more obvious.

The invention relates to the technical field of biological engineering, in particular to metabolically modified CAR-T cells and a preparation method and application thereof. According to the method, through biological orthogonal metabolic modification, the CAR-T cells can be effectively targeted and drawn to a tumor site in vivo; in-vivo targeting and drawing leads to combination of specific CAR molecules on the CAR-T cells with tumor antigens on the tumor site, correspondingly the specific activation of the CAR-T cells is stimulated, and a more efficient tumor killing capability is achieved.

The present invention discloses a method for splitting Cas9 and an application. The present invention provides a method for splitting Cas9 protein at a plurality of sites of the Cas9 protein into different segments of amino acid sequences. The Cas9 protein is spCas9(D10A) protein. The split Cas9 protein can be transported into target cells/vectors more conveniently, and can be recombined by meansof intein. Accordingly, the present invention provides proteome and fusion proteome obtained by the method and nucleic acid constructs and vectors for expressing the fusion proteome, and engineering cells. The fusion proteome and the vectors can be used for editing cytogenes, or in targeted positioning or gene expression transcription activation or gene expression transcription inhibition, and used in preparation of pharmaceutical preparations for gene editing.

The present invention relates to a method for manufacturing monascus fermented purplish red rice koji soybean sauce by using soybean residues and sauce residues. The method comprises the following steps: (1) raw material pre-treating; (2) monascus fermented purplish red rice koji powder manufacturing: a, monascus liquid preparing; b, inoculating; c, fermenting; and d, drying; (3) soaking; (4) cooking; (5) cooling and inoculating; (6) koji manufacturing; and (7) fermenting to prepare the monascus fermented purplish red rice koji soybean sauce. The method utilizes parts of soybean residues and sauce residues to replace parts of soybeans and flour raw materials, combines aspergillus oryzae and monascus compound koji manufacturing with the fermentation technology to prepare the novel monascusfermented purplish red rice koji soybean sauce, turns waste into treasure, reduces resource waste and environmental pollution, improves the utilization rate of the raw materials, reduces cost, provides a new way to solve a problem of high value reuse of the sauce residues and soybean residue resources, makes full use of grain resources, also enriches the flavor of the finished product sauce, and also makes up for defects of the artificial fermented soybean sauce.

The invention provides an externally applied traditional Chinese medicine composition for treating knee osteoarthritis (OA). The composition is prepared from fructus gardeniae, safflower, angelica sinensis, panax pseudo-ginseng, dipsacus root, rheum officinale, peach kernels, danshen, radix angelicae and the rhizome of chuanxiong at a mass ratio of 2:2:4:3:4:5:3:4:4:3. The composition has the following beneficial effects: the composition uses monarch, minister, assistant and guide to compose the prescriptions and is ground into powder by utilizing the traditional acupoint therapy with traditional Chinese medicines and the modern pharmaceutical transdermal absorption theory; yellow wine is added to the powder to be mixed uniformly to prepare paste and the paste is applied to hedings on the two sides of the human bodies and internal and external knee eyes; qi and blood of tendons and vessels are smoothened by stimulating acupoints, conducting channels, dredging channels and activating blood and removing stasis, thus achieving the aim of treatment; the medicine is convenient to administrate; the efficacy is lasting; the adverse reactions are less; the cure and obvious effective rates are 18.0%; the effective rate is 72.0%; and the total effective rate is 90%; therefore the composition is an effective medicine for treating knee OA.

The invention discloses an external medicine for treating gall caused by gout, rheumatism and rheumatoid arthritis and a preparation method thereof. The external medicine comprises the following medicinal raw materials in the optimum mass ratio: 90g of radix aconiti kusenzoffii, 90g of radix aconite, 90g of nux vomica, 90g of thunder god vine, 80g of notopterygium root, 80g of radix angelicae pubescentis, 60g of ephedra, 80g of cassia twig, 60g of asarum produced from Liaoning Province, 90g of radix clematidis, 80g of rhizoma atractylodis, 80g of phellodendron chinense schneid, 60g of pepper, 80g of short-pedicel aconite root, 80g of angelica, 90g of ligusticum wallichii, 60g of pedate pinallia jackinthepulpit rhizome, 80g of fructus broussonetiae, 60g of scorpion, 40g of centipede, 80g of ground beetle, 80g of frankincense, 80g of myrrh, 90g of medicinal cyathula root and 80g of borneol. The invention discloses a preparation method of an external tincture for treating gall caused by gout and rheumatism and rheumatoid arthritis, wherein the preparation method comprises technological processes of weighing on the medicinal raw materials according to the optimum mass ratio, putting into a container, soaking in alcohol, filtering, processing dregs, precipitating and bottling. The external medicine disclosed by the invention is obvious in efficacy, and the effective ratio of relieving swelling and pain is high up to 95%; and the external medicine is free from drug dependence, convenient to carry and use, low in cost and simple in production process.

The invention provides application of water-soluble pearl powder to promotion of wound healing. The water-soluble pearl powder has the new usage for repairing skin wound. A preparation method of the water-soluble pearl powder comprises the following steps that S1, pretreatment is conducted; S2, acid modification is conducted; and S3, enzymatic modification is conducted, specifically, liquid obtained in the S2 is subjected to enzymolysis, after enzymolysis is completed, enzyme deactivation is conducted at 100 DEG C for ten minutes, centrifuging is conducted, supernatant is taken, subjected to freeze drying and crushed till the grain size being 200 meshes or above, and the water-soluble pearl powder is obtained. The invention provides the new usage of the water-soluble pearl powder to skin wound drugs for repairing skin burns, wounds, ambustion, scalds and the like, the effect of the water-soluble pearl powder is obviously better than that of drugs in the prior art, wound repairing is quick, scars are avoided, and the wound repairing promoting effect is more obvious.

The invention discloses ocellated puffer liver oil Chinese herbal health care oral liquid capable of resisting tumors and enhancing immunity. In terms of 1000 parts, the health care oral liquid consists of the following Chinese herbal raw materials: 4-6 parts of ocellated puffer liver oil, 10-15 parts of fructus arctii, 8-12 parts of houttuynia cordata, 10-15 parts of radix puerariae, 15-20 parts of rhizoma ligustici wallichii, 10-13 parts of cortex phellodendri, 8-12 parts of herba andrographitis, 10-15 parts of dandelion, 10-16 parts of fruit of Chinese wolfberry, 10-18 parts of cyrtomium rhizome, 5-10 parts of rhizoma polygoni cuspidati, 5-10 parts of radix achyranthis bidentatae, 10-15 parts of liquorice root and 10-15 parts of chlorella vulgaris, and the health care oral liquid is prepared through combined technologies of ultrasonic-assisted extraction, volatile oil distillation, water decoction and alcohol extraction. The product has the advantages of being good in functions of resisting tumors and enhancing immunity, safe and free from side effects, convenient to drink and the like.

The invention discloses a rose health beverage having efficacy for delaying senility. The rose health beverage is prepared by scientific compatibility of rose and other Chinese herbal medicine raw materials. Preferably, the rose health beverage is prepared from the raw materials in parts by mass: 20-30 parts of rose, 20-30 parts of wolfberry, 5-10 parts of dendrobium nobile, 5-10 parts of dioscorea rhizome, 2-5 parts of liquorice, 10-15 parts of white atractylodes rhizome, 3-5 parts of eleutherococcus senticosus, 3-5 parts of prepared rehmannia root, 2-3 parts of pseudo-ginseng, 3-5 parts of rhodiola rosea and 3-5 parts of stevia rebaudiana, and is prepared through the processes of enzymolysis, extraction, mixing, blending, filling and sterilization. The rose health beverage has the advantages that through synergistic effect of the rose, the wolfberry and other Chinese herbal medicines, the rose health beverage has the remarkable effects of resisting senility, tonifying qi and benefiting body, supporting the healthy energy, resisting fatigue, increasing immunnity, prolonging life and the like, is safe without side effect, is convenient to eat, and has relatively good market application prospects.

The invention mainly relates to a cigarette for smoking, in particular to a health cigarette which is safe and good for improving the function of a human brain. The health cigarette for improving the function of the human brain is prepared from the following materials by a preparation method: 5-15 percent of tatarinowii sweetflag rhizome powder and 85-95 percent of tobacco shred are mixed or a tatarinowii sweetflag rhizome tincture is prepared into the tobacco shred containing the tatarinowii sweetflag rhizome component by a method of adding auxiliary materials and additives into the tobacco, and under the condition of not increasing the length of cigarette, moxa with the thickness of 5mm is added between the tobacco shred and a shed bundle filter tip, and cigarette-enclosing paper is used on a cigarette-enclosing machine so as to form and box the cigarette. At present, in human clinical trials, about forty types of medicines for improving cognition exist in total. The medicines aim at boosting the spirit, improving the attention and the memory and improving the decision-making and planning capability. Although the side effect is strong, many people take the medicines still.

The present invention provides a composition for preventing, suppressing, alleviating, ameliorating or treating pruritus comprising stem cell-derived exosomes as an active ingredient. The composition of the present invention is able to act against pruritus-inducing multiple cytokine targets, for example, IL-4, IL-31 and TSLP, and thus is able to be widely applied against pruritus caused by various factors and is able to effectively suppress and alleviate pruritus. In addition, when the composition of the present invention is applied directly to human skin, it is able to remarkably ameliorate pruritus-associated clinical scores, erythema and the like. Thus, the composition of the present invention is able to be used as a pharmaceutical composition, a skin external preparation and a cosmetic composition for preventing, suppressing, alleviating, ameliorating or treating pruritus.