This invention belongs to the field of
plant tissue culture technology, specifically disclosing a method for producing
Polygonatum polysaccharides using
Polygonatum yunnanense
tissue culture, comprising the following steps: explant disinfection,
callus induction,
callus proliferation and induction culture: selecting vigorous
callus tissue, transferring it to a culture medium: White medium + 2,4-D 1.5 mg / L + VC 20 mg / L +
sucrose 40 g / L + 30 mg / L
inducer +
agar 6 g / L, pH 5.8, after culture, collecting the callus,
drying it, and then extracting
Polygonatum yunnanense polysaccharides; the
inducer is Polygonatum yunnanense endophytic basidiomycetes (… Talaromyces sp. The
fermentation broth extract of HJF23; the HJF23 preservation number is CCTCC NO: M2021344. This invention not only realizes the efficient
biosynthesis of Polygonatum yunnanense
polysaccharide, but also significantly enhances its
functional activity.