The invention relates to a preparation method of rice bran dietary fiber, which comprises the following steps that: (1) rice bran is crushed, passes through a screen which is more than or equal to 30mesh, is added with water to be stirred uniformly, and stays still for 30 to 50min; (2) the prepared water-absorbing swelling rice bran material is added with dilute hydrochloric acid, the pH value is adjusted to be 4.5 to 5.5, and then 1.0 to 1.5 percent of hot temperature resistant amylase is added to be heated to 85 to 100DEG C, and the temperature is maintained for 30 to 40min; (3) dilute sodium hydroxide or potassium hydroxide solution is added, the pH value is adjusted to be 7.5 to 8.5, 1.0 to 1.5 percent of protease with the enzyme activity more than or equal to 500U / g to be heated to 45 to 55 DEG C, and the temperature is maintained for 60 to 90min; (4) the intermediate product is pressed, filtered and washed by water; (5) diluted phosphoric acid is added in to adjust the pH value to be 5.0 to 5.5, 0.1 to 0.2 percent of cellulose is added in to be heated to 45 to 55DEG C, and the temperature is maintained for 50 to 70min; (6) 2 to 5 percent of hydrogen peroxide is added into a sealed container to be stirred for 20 to 30min; and (7) and the product is dried and crushed. The dietary fiber prepared by the invention has delicate taste without roughness, and can serve as the additive in medicine, health food, bread, biscuits, drinks and the like.