Rice bran water solution extract as well as preparation method and use thereof
An extract and rice bran technology, applied in the field of rice bran aqueous solution extract and its preparation, can solve the problems of heavy environmental burden, high degree of protein denaturation, and large amount of waste water
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[0032] The present application also provides a preparation method of rice bran water-soluble extract containing high functional rice bran glycoprotein, comprising the following steps:
[0033] 1) Weigh a certain amount of degreased rice bran with a particle size of <150 μm, add it to deionized water, stir for 10-60 minutes, and make a rice bran suspension with a material-to-liquid ratio of 1:5-1:20. Balance energy consumption and yield Angle, preferably 1:6-1:12;
[0034] 2) Add 0.1%-0.5% amylase to the rice bran suspension in step 1), including high-temperature amylase, medium-temperature amylase, etc., preferably high-temperature amylase, and the dosage is preferably 0.1%-0.3%;
[0035] 3) If a medium-temperature amylase is added in step 2), put the sample into a water bath shaker with a rotation speed of 150-250rpm, a reaction temperature of 50-70°C, preferably 50-60°C; an enzymatic hydrolysis time of 60-150min, preferably 90 -140min;
[0036] 4) If high-temperature amyla...
Embodiment 1
[0078] Weigh 50g of defatted rice bran with a particle size of 100μm into a conical flask, add 400g of deionized water, and stir for 10 minutes to obtain a rice bran suspension. Place the sample in a water bath shaker at 90°C, add 0.5% high-temperature amylase, and react for 20 minutes , and then lower the temperature to 55° C., adjust the suspension to pH 4, further add 0.5% viscozyme and 0.5% phytase, and react for 2 hours to obtain a rice bran suspension. The suspension was adjusted to pH 8.5, then transferred to a hydrothermal kettle, heated to 115°C, reacted for 2 hours, cooled and then centrifuged, the supernatant was collected and freeze-dried to obtain product E1.
Embodiment 2
[0080] Weigh 50g of defatted rice bran with a particle size of 100μm into an Erlenmeyer flask, add 300g of deionized water, and stir for 10 minutes to obtain a rice bran suspension. Place the sample in a water bath shaker at 90°C, add 0.1% high-temperature amylase, and react for 20 minutes , and then lower the temperature to 55° C., adjust the suspension to pH 4, further add 0.1% viscozyme and 0.1% phytase, and react for 2 hours to obtain a rice bran suspension. The suspension was adjusted to pH 8.5, then transferred to a hydrothermal kettle, heated to 115°C, reacted for 2 hours, cooled and then centrifuged, the supernatant was collected and freeze-dried to obtain product E2.
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