Method for co-producing rice bran dietary fiber and rice bran protein by utilizing defatted rice bran
A technology of degreased rice bran and dietary fiber, which is applied in food preparation, protein food processing, plant protein processing, etc., can solve the problems of a large gap in the application of rice bran, and the simultaneous preparation process of rice bran protein and rice bran dietary fiber has not been found, so as to improve the economy. Benefits and social benefits, the effect of reducing production costs and improving utilization
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Embodiment 1
[0031] A method for preparing rice bran dietary fiber and rice bran protein by co-production of defatted rice bran, comprising the following process steps:
[0032] (1) Crush: Crush the defatted rice bran and filter through an 80-mesh sieve;
[0033] (2) Soaking: Soak the crushed defatted rice bran in NaOH solution with a concentration of 0.15mol / L for 60 minutes to obtain the soaked product;
[0034] (3) Centrifugation: Centrifuge the soaked material with a centrifuge speed of 4500r.min-1 and a centrifugation time of 10min to obtain the first filter residue and the first protein filtrate;
[0035] (4) Compound enzymatic hydrolysis:
[0036] (a) Mix the substrate and buffer evenly to obtain a bottom solution. The substrate is defatted rice bran, and the buffer is a phosphate buffer solution of sodium dihydrogen phosphate and disodium hydrogen phosphate with a concentration of 0.1 mol / L by weight. The weight percentage concentration is 21%, and then the first filter residue a...
Embodiment 2
[0042] A method for preparing rice bran dietary fiber and rice bran protein by co-production of defatted rice bran, comprising the following process steps:
[0043] (1) Crush: Crush the defatted rice bran and filter through a 100-mesh sieve;
[0044] (2) Soaking: Soak the crushed defatted rice bran in NaOH solution with a concentration of 0.15mol / L for 45 minutes to obtain the soaked product;
[0045] (3) Centrifugation: Centrifuge the soaked material with a centrifuge speed of 4500r.min-1 and a centrifugation time of 8min to obtain the first filter residue and the first protein filtrate;
[0046] (4) Compound enzymatic hydrolysis:
[0047] (a) Mix the substrate and buffer evenly to obtain a bottom solution. The substrate is defatted rice bran, and the buffer is a phosphate buffer solution of sodium dihydrogen phosphate and disodium hydrogen phosphate with a concentration of 0.1 mol / L by weight. The weight percentage concentration is 19%, and then the first filter residue and ...
Embodiment 3
[0053] A method for preparing rice bran dietary fiber and rice bran protein by co-production of defatted rice bran, comprising the following process steps:
[0054] (1) Crush: Crush the defatted rice bran and filter through a 90-mesh sieve;
[0055] (2) Soaking: Soak the crushed defatted rice bran in NaOH solution with a concentration of 0.15mol / L for 50 minutes to obtain the soaked product;
[0056] (3) Centrifugation: Centrifuge the soaked material with a centrifuge speed of 4500r.min-1 and a centrifugation time of 9min to obtain the first filter residue and the first protein filtrate;
[0057] (4) Compound enzymatic hydrolysis:
[0058] (a) mix the substrate and the buffer evenly to obtain the bottom solution, the substrate is defatted rice bran, the buffer is potassium dihydrogen phosphate buffer solution with a weight percent concentration of 0.1mol / L, and the weight percent concentration of the bottom solution is 20%, Then add the first filter residue and compound enzy...
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