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Preparation method and application of bioactive bracket with chemotactic function

A biologically active and functional technology, applied in the field of scaffold materials for repairing spinal cord injury, to achieve the effect of improving the microenvironment and improving survival

Active Publication Date: 2015-07-29
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, at home and abroad, a scaffold material that has biological activity and can chemoattract endogenous cells to migrate to spinal cord injury / transplantation has not been reported yet.

Method used

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  • Preparation method and application of bioactive bracket with chemotactic function
  • Preparation method and application of bioactive bracket with chemotactic function
  • Preparation method and application of bioactive bracket with chemotactic function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] The main instrument used in the present invention, chemotactic active support and reagent are described in detail below by specific examples:

[0021] 1. Main instruments

[0022] Ultra-clean workbench (Suzhou Purification Electronic Equipment Factory); ordinary centrifuge (Kubota Japan); constant temperature water bath box (Beijing Medical Equipment Factory); 5% CO 2 Incubator (Queue USA); Inverted Phase Contrast Microscope (Olympus Japan); Fluorescence Microscope (Leica Germany); Scanning Electron Microscope (Philips Netherlands); Transmission Electron Microscope (Philips Netherlands); Laser Confocal Imaging System (Carl Zeiss Germany); Low Temperature Oven ( Shanghai Yuejin Medical Instrument Factory); high temperature oven (Shanghai Yuejin Medical Instrument Factory); high pressure sterilizer (Jiangyin Binjiang Medical Equipment Factory); constant cold box slicer (Shandon UK); ultrapure water instrument (Molsheim France); ELISA Detector (Bio-Rad USA); electrophores...

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PUM

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Abstract

The invention discloses a novel bioactive bracket with a chemotactic function, which is used for endogenous cell migration, and promotion of endogenous cells to survive and differentiate for repairing spinal cord injury, and has a rear nerve protection effect. The preparation method comprises the following steps: by taking natural silk-fibroin extracted from silkworm cocoon as a medium, transforming a neurotrophic factor-3(NT-3), retinoic acid (RA) and a silk-fibroin crystalline form by utilizing a freeze-drying technology and an alcohol cross-linking agent, cross-linking and fixing in a three-dimensional gelatin sponge bracket. The bioactive bracket with the chemotactic function provided by the invention can release the chemotactic factor to attract endogenous cell migration to an injury / transplantation area with a relatively long time after being transplanted to spinal marrow, and can release a nutritional factor with a nerve protection effect to improve a microenvironment, so that the purpose of attracting endogenous cells to perform self-repair of the spinal cord injury is achieved.

Description

Technical field [0001] The patent of the present invention relates to a scaffold material for repairing spinal cord injury, especially a bioactive scaffold that can attract endogenous cell migration and promote endogenous cell survival and differentiation. Background technique [0002] Currently, known scaffolds for repairing spinal cord injuries are composed of scaffolds and exogenous cells. It mainly achieves the effect of replacing lost neurons and treating spinal cord injury by transplanting exogenous cells into the injured area, while the scaffold is only used as a carrier for supporting cell attachment. However, in the process of clinical application, the transplantation of exogenous cells faces many difficulties such as source, ethics and immune rejection, which greatly limits the treatment of spinal cord injury with exogenous cell transplantation. Therefore, it is the key to find an effective way to circumvent the cell source problem of cell transplantation and meet...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/54
Inventor 曾园山李戈
Owner SUN YAT SEN UNIV
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