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Method for producing feed microecological preparation by synergic fermentation of probiotics

A micro-ecological preparation and collaborative fermentation technology, which is applied in the fields of application, animal feed, animal feed, etc., can solve the problems of low feed conversion rate and production performance, high toxicity and side effects, and weak disease resistance, so as to improve the intestinal microenvironment , promote animal growth, improve the effect of protein utilization

Inactive Publication Date: 2013-07-10
杨辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above situation, in order to solve the defects of the prior art, the purpose of the present invention is to provide a method for the production of feed microecological preparations through the synergistic fermentation of probiotics, which can effectively solve the problem of low feed conversion rate and production performance in the prior art, and low disease resistance. Weak, high toxicity and side effects

Method used

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  • Method for producing feed microecological preparation by synergic fermentation of probiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] With Bacillus subtilis, Lactobacillus plantarum and Candida as bacterial strains, Bacillus subtilis, Lactobacillus plantarum and Candida are all inserted into the fermenter according to the 5% inoculation amount of the medium volume and mixed evenly, and the medium is By weight: 96% chrysanthemum meal, 0.8% astragalus, 0.8% forsythia, 0.8% houttuynia, 0.8% hawthorn and 0.8% tangerine peel, mixed and evenly crushed through a 60-mesh sieve, first carried out aerobic ventilation fermentation, fermentation The temperature is 30°C, the ventilation rate is 0.5L / min, the ventilation is stopped for 10min every 30min, the stirring speed is 45r / min, cultivated for 28h, and then anaerobic fermentation is carried out, the fermentation temperature is 32°C, the stirring speed is 20r / min, and cultivated for 24h.

Embodiment 2

[0011] With Bacillus subtilis, Lactobacillus plantarum and Candida as bacterial strains, Bacillus subtilis, Lactobacillus plantarum and Candida are all inserted into the fermenter according to the 7.5% inoculation amount of the medium volume and mixed evenly, and the medium is By weight: 95% chrysanthemum meal, 1% astragalus, 1% forsythia, 1% houttuynia, 1% hawthorn and 1% tangerine peel, mixed and evenly crushed through an 80-mesh sieve, first aerobic ventilation fermentation, fermentation The temperature is 33°C, the ventilation rate is 0.8L / min, the ventilation is stopped for 10min every 30min, the stirring speed is 52r / min, cultivated for 34h, and then anaerobic fermentation is carried out, the fermentation temperature is 33°C, the stirring speed is 30r / min, and cultivated for 36h.

Embodiment 3

[0013] With Bacillus subtilis, Lactobacillus plantarum and Candida as bacterial strains, Bacillus subtilis, Lactobacillus plantarum and Candida are all inserted into the fermenter according to the 10% inoculation amount of the medium volume and mixed evenly, and the medium is By weight: 94% chrysanthemum meal, 1.2% astragalus, 1.2% forsythia, 1.2% Houttuynia cordata, 1.2% hawthorn and 1.2% tangerine peel, mixed evenly and crushed through a 100-mesh sieve, first carried out aerobic ventilation fermentation, fermentation The temperature is 35°C, the ventilation rate is 1.2L / min, the ventilation is 30min, stop for 10min, the stirring speed is 60r / min, cultivate for 40h, and then carry out anaerobic fermentation, the fermentation temperature is 35°C, the stirring speed is 40r / min, and cultivate for 48h.

[0014] The relevant test data are as follows:

[0015] 1. Shape and color

[0016] The semi-solid is brownish-yellow or taupe; the liquid is brownish-yellow.

[0017] 2. O...

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Abstract

The invention relates to a method for producing a feed microecological preparation by the synergic fermentation of probiotics, and the method can effectively solve the problems of low conversion ratio and production performance, weak disease resistance and strong toxic and side effects of feeds in the prior art. According to the technical scheme, bacillus subtilis, lactobacillus plantarum and candida are taken as bacterial strains and are inoculated and mixed evenly in a fermentation tank according to the inoculation quantity which is equal to 5%-10% of the volume of a culture medium, wherein the culture medium is prepared by mixing evenly 94%-96% of chrysanthemum meal, 0.8%-1.2% of Astragalus mongholicus, 0.8%-1.2% of forsythia, 0.8%-1.2% of Houttuynia cordata, 0.8%-1.2% of hawthorn and 0.8%-1.2% of pericarpium citri reticulatae by weight percentage and crushing the mixture to 60-100 meshsieves; oxygen ventilation fermentation is performed firstly, wherein the fermentation temperature is 30-35 DEG C, the ventilatory capacity is 0.5-1.2 L / min, the ventilation is stopped for 10 minutes every 30 minutes, the stirring speed is 45-60 r / min, and the culture lasts for 28-40 hours; then, anaerobic fermentation is performed, wherein the fermentation temperature is 32-35 DEG C, the stirring speed is 20-40 r / min, and the culture lasts for 24-48 hours; and finally, the feed microecological preparation is obtained. The feed microecological preparation can increase the utilization rate of protein and promote the growth of animals and is innovation of feed additives.

Description

technical field [0001] The invention relates to the field of biological fermentation, in particular to a method for probiotics cooperative fermentation to produce feed microecological preparations. Background technique [0002] With the continuous development of economy and society, people's quality of life is gradually improving, not only requires food rich in nutrition, flavor, safety and hygiene, but also requires a good living environment, but the breeding industry that is closely related to this has encountered many new problems: such as The prevention and control of livestock and poultry diseases has become more difficult. Avian influenza and swine unidentified high fevers have occurred from time to time. The problem of sewage treatment in the breeding industry is becoming more and more serious, and the safety and flavor problems of animal-derived food. In particular, frequent food safety accidents in recent years have continuously stimulated the nerves of Chinese peo...

Claims

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Application Information

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IPC IPC(8): A23K1/16
Inventor 杨辉
Owner 杨辉
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