Growth factor porous micro-sphere compound system coated by injectable hydrogel

A growth factor and porous microsphere technology, applied in the field of biomedical materials for nerve tissue repair, can solve the problems of too fast release of growth factors, short action period, etc., to avoid direct contact, uniform and slow release, good encapsulation and fixation Effect

Inactive Publication Date: 2016-02-03
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The composite system can degrade and slowly release growth factors under physiological conditions, realizing the slow release of growth factors in the body, solving the problems of too fast release of growth factors and too short action period, so as to achieve the effect of nerve repair

Method used

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  • Growth factor porous micro-sphere compound system coated by injectable hydrogel
  • Growth factor porous micro-sphere compound system coated by injectable hydrogel
  • Growth factor porous micro-sphere compound system coated by injectable hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Preparation of a Growth Factor Porous Microsphere Composite System Encapsulated by Injectable Hydrogel

[0045] (1) Dissolve 0.5g HA in 100ml of ultrapure water at 0-4°C, stir magnetically to fully dissolve, then add 2gADH, then add 0.8gEDC and 0.7gNHS, 1mol / lHCl to adjust the pH to 5.0, react for 20h, and dialyze That is to get HA-ADH sol;

[0046] (2) 1.5g of PLLA was dissolved in 20mL of dichloromethane, then added to NGF, bFGF (NGF4.5mg / ml; bFGF was 0.5mg / ml) aqueous phase containing 1g of sodium carbonate and 1.5g of PVA, and stirred Or form an O / W emulsion under ultrasonic conditions, then add 3ml of 26.7% PVA external water phase to it, stir at high speed for a period of time to form a double emulsion, transfer the double emulsion to a beaker, and stir at 300rpm for 80min to solidify. Collect the precipitate by centrifugation and keep the supernatant aqueous phase, wash three times and keep the washing liquid, then freeze-dry the precipitate for 24 hours;

[00...

Embodiment 2

[0067] Preparation of a Growth Factor Porous Microsphere Composite System Encapsulated by Injectable Hydrogel

[0068] (1) Dissolve 1.0g HA in 100ml of ultrapure water at 0-4°C, stir magnetically to fully dissolve, then add 2.0gADH, then add 1.6gEDC and 1.4gNHS, 1mol / lHCl to adjust the pH to 4.5, react for 19h, Dialysis to obtain HA-ADH sol;

[0069] (2) 2.25g of PLA was dissolved in 20mL of dichloromethane, then added to NGF, bFGF (NGF7.875mg / ml; bFGF is 0.75mg / ml) aqueous phase containing 2.0g sodium carbonate and 3.0gPVA, in Form an O / W emulsion under stirring or ultrasonic conditions, then add 6ml of 26.7% PVA external water phase to it, stir at high speed for a period of time to form a double emulsion, transfer the double emulsion to a beaker, stir at 300rpm for 100min to solidify. Collect the precipitate by centrifugation and keep the supernatant aqueous phase, wash three times and keep the washing liquid, then freeze-dry the precipitate for 36 hours;

[0070] (3) At 0...

Embodiment 3

[0089] Preparation of a Growth Factor Porous Microsphere Composite System Encapsulated by Injectable Hydrogel

[0090] (1) Dissolve 1.0g HA in 100ml ultrapure water under the condition of 0-4℃, magnetically stir until fully dissolved, then add 3.0gADH, then add 1.6gEDC and 1.4gNHS, 1mol / lHCl to adjust the pH to 5.5, react for 21h, Dialysis to obtain HA-ADH sol;

[0091] (2) 1.5g of PLA was dissolved in 20mL of dichloromethane, then added to NGF, bFGF (NGF9.5mg / ml; bFGF was 1mg / ml) aqueous phase containing 1g of sodium carbonate and 1.5g of PVA, stirring or Form an O / W emulsion under ultrasonic conditions, then add 3ml of 26.7% PVA external water phase to it, stir at high speed for a period of time to form a double emulsion, transfer the double emulsion to a beaker, and stir at 300rpm for 110min to solidify. Collect the precipitate by centrifugation and keep the supernatant aqueous phase, wash three times and keep the washing liquid, and then freeze-dry the precipitate for 30 ...

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Abstract

The invention relates to the preparation of a growth factor porous micro-sphere compound system coated by an injectable hydrogel. The preparation is characterized by comprising a first component, namely HA-ADH sol obtained by cross-linking of hyaluronic acid (HA) and adipic dihydrazide (ADH), and polylactic acid (PLLA) porous micro-spheres which are carried with growth factors and loaded in the first component. The compound system is capable of degrading and slowly releasing the growth factors under physiological conditions, so that the slow releasing of the growth factors in a body is realized, and the problems that the growth factors are released too fast and an action period is too short are solved, thereby reaching the effect of neural restoration.

Description

technical field [0001] The invention belongs to the field of biomedical materials for nerve tissue repair, in particular to a composite system of growth factor porous microspheres wrapped by injectable hydrogel. Background technique [0002] Peripheral nerve defect and repair has always been one of the diseases that plague many people in the world, mostly caused by transverse injury, burn or degenerative disease. Peripheral nerve injuries can be divided into two categories: non-defective stump injuries and stump-defective injuries. For the damage without defects between the broken ends, surgical methods such as adventitial anastomosis and perineural anastomosis can be used for anastomosis repair. However, the repair and functional reconstruction of long peripheral nerve defects is still one of the clinical problems, seriously affecting the quality of life of patients. Since the last century, the use of non-biological catheters to treat peripheral nerve defects has become a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18A61K9/06A61K47/36A61K47/34A61P25/00A61P25/02
Inventor 徐海星李燕许沛虎张凌溪李依萍郭兴蕾黄志军
Owner WUHAN UNIV OF TECH
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