Calcium phosphate bioactive scaffold capable of regulating and controlling cell adhesion performance

A technology of calcium phosphate and cell adhesion, which is applied in tissue regeneration, ceramic products, prostheses, etc., achieving good market application prospects, promoting cell adhesion, and simple process methods

Inactive Publication Date: 2022-03-22
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current study, the mechanism of the influence of the micro-nano-scale surface topography on the surface of the material on the cells is not yet fully understood.

Method used

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  • Calcium phosphate bioactive scaffold capable of regulating and controlling cell adhesion performance
  • Calcium phosphate bioactive scaffold capable of regulating and controlling cell adhesion performance
  • Calcium phosphate bioactive scaffold capable of regulating and controlling cell adhesion performance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Extrusion 3D printing equipment was used to prepare ceramic repair brackets. Firstly, 10% alcohol solution of polyvinyl butyral (PVB, molecular weight: 170000-250000) and hydroxyapatite powder are mixed evenly with a defoamer. Put the mixed ceramic slurry into the printing cylinder for 3D printing. Among them, the printing speed is 8mm / s, the printing layer thickness is 0.32mm, the extrusion pressure is 0.3MPa, and the pore spacing is 1mm.

[0031] (2) The printed hydroxyapatite scaffold was sintered at high temperature in a muffle furnace to remove the binder inside the scaffold and solidify the ceramic scaffold. The sintering temperature used was 1200° C. and the sintering time was 2 hours. After sintering, the scaffold had a macroscopic porosity of 69% and an overall shrinkage of 35%.

[0032] (3) The calcium phosphorus micro-nano active coating of the scaffold was prepared by hydrothermal method to increase the adhesion and recruitment of sintered ceramics to...

Embodiment 2

[0036] Tricalcium phosphate was selected as the printing material. According to the method of Example 1, the base part of the stent was prepared by 3D printing, and then the calcium phosphorus micro-nano active coating on the surface was prepared by a hydrothermal method. The selection of other parameters and the preparation process were the same as in Example 1, except that the water The heat medium is HNO with a pH of 4 3 solution. Finally, a ceramic stent with a micron-sized calcium-phosphorus active coating is obtained. Since tricalcium phosphate is easy to release a large amount of calcium ions in an acidic solution, a ceramic stent with a coating can be obtained only by using an acidic solution as a hydrothermal medium. Its coated rod-like structure is about 50 μm in length.

Embodiment 3

[0038] Biphasic calcium phosphate is selected as the printing material. According to the method of Example 1, the base part of the stent was prepared by 3D printing, and then the surface coating was prepared by the hydrothermal method. The selection of other parameters and the preparation process were the same as in Example 1, except that the pH of the hydrothermal medium was selected to be 4HNO 3 solution. The end result is a ceramic stent with a micron-sized coating. Since the tricalcium phosphate inside the biphasic calcium phosphate is easy to release a large amount of calcium ions in an acidic solution, a ceramic stent with a coating can be obtained only by using an acidic solution as a hydrothermal medium. Its coated rod-like structure is about 5 μm in length.

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Abstract

The invention relates to a calcium phosphate ceramic active scaffold capable of regulating and controlling cell adhesion performance. According to the method, the calcium phosphate micro-nano active coating is individually designed on the surface of the calcium phosphate biological ceramic scaffold, so that the adhesion behavior of cells is accurately regulated and controlled, and the repair process of tissues is regulated and controlled. According to the preparation method, the calcium phosphate ceramic bracket can be obtained through processes such as 3D printing, a foaming method and a tabletting method, and the surface calcium-phosphorus micro-nano active coating is obtained through processes such as hydrothermal treatment, mineralization and electro-deposition. The design accurately acts on the adhesion protein of the cells at the micro-nano level, so that the change of the cell adhesion behavior is realized. According to the invention, the regulation and control effects of the material on adhesion, activity, proliferation and differentiation of cells are obviously enhanced, and the material has higher hydrophilicity, protein adsorbability, stem cell collection and tissue repair promotion capabilities.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, and discloses a calcium phosphate bioactive scaffold capable of regulating cell adhesion performance. Background technique [0002] Bone defects caused by bone trauma, bone aging, bone tumors and other diseases are increasing day by day. There are three main materials currently used in bone repair: autologous bone, allogeneic bone, and bone tissue engineering. Due to the limited source of autologous bone and severe immune rejection of allograft bone, the application of bone tissue engineering has become more and more extensive. Among them, bioactive ceramics are a good material for bone repair, and it has been found in research that bioactive ceramics with certain pores (usually 200 μm to 600 μm) have a certain osteoinductive ability, which brings infinite hope to the field of bone tissue regeneration. . Bioceramics used for bone repair generally require certain mechanical propert...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C04B38/06C04B35/447C04B35/622C04B41/81A61L27/10A61L27/12A61L27/54A61L27/56
CPCC04B38/067C04B35/447C04B35/622C04B41/81C04B41/5015C04B41/009A61L27/12A61L27/10A61L27/56A61L27/54C04B2235/6567C04B2235/6026A61L2430/02C04B38/0074C04B41/46C04B41/4535C04B41/0072
Inventor 周长春吴丽娜张勃庆苏子璇桂兴宇王科锋范洪远蒋青樊渝江张兴栋
Owner SICHUAN UNIV
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