Collagen composite bioactive stent and preparation method thereof

A collagen, bioactive technology, applied in the preparation of the bioactive scaffold, based on the composite bioactive scaffold of CSCI (chlorinated chitosan) and collagen, in the field of bioactive scaffolds, can solve the lack of mechanical strength and physical and chemical properties , poor biological activity, and a single component, to achieve good degradability, less toxic residues, and excellent physical and chemical properties

Inactive Publication Date: 2019-08-23
SHANXI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the scaffold material involved in this patent has a single component, lacks controlla

Method used

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  • Collagen composite bioactive stent and preparation method thereof
  • Collagen composite bioactive stent and preparation method thereof
  • Collagen composite bioactive stent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Cut the tails of C57BL / 6J mice (male or female) from the base of the tails, wash them with tap water and distilled water in sequence, and soak them in 75% ethanol for 15-20 minutes for disinfection.

[0043] Surgical scissors cut off the tip of the rat tail by 1-2mm, peeled off the skin from the root, and extracted the silvery white tendon with tweezers in the direction from the root of the tail to the tip of the tail to avoid extracting muscle tissue.

[0044] The extracted tendon was placed in a 100mL beaker, washed with normal saline and weighed. To avoid denaturation of the collagen contained in the rat tail tendon, this step was strictly performed on an ice bath.

[0045] Cut the silver-white tendon with surgical scissors (the more broken the better), put it in a 50mL centrifuge tube, add 50mL of 0.1% acetic acid solution per gram of rat tail tendon, shake it to make it evenly distributed, place it at 4°C for 48-72h, and shake it from time to time , fully dissolve...

Embodiment 2

[0058] Under an ice bath, take 14.2 mL of the temperature-sensitive CSCI-β-GP solution prepared in Example 1, and evenly add it to 2 mL of the type I rat tail collagen solution prepared in Example 1, stir until fully mixed, and pour into a glass test tube , placed in a 37°C cell incubator and fixed into a gel, and the CSCI-Ⅰ type rat tail collagen composite bioactive scaffold was prepared.

[0059] The average gelation time of the bioactive scaffold prepared in this example was 5.98 min.

Embodiment 3

[0061] Under an ice bath, take 12.4 mL of the temperature-sensitive CSCI-β-GP solution prepared in Example 1, and evenly add it to 2 mL of the type I rat tail collagen solution prepared in Example 1, stir until fully mixed, and pour into a glass test tube , placed in a 37°C cell incubator and fixed into a gel, and the CSCI-Ⅰ type rat tail collagen composite bioactive scaffold was prepared.

[0062] The average gelation time of the bioactive scaffold prepared in this example was 5.81 min.

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Abstract

The invention discloses a collagen composite bioactive stent and a preparation method thereof. The collagen composite bioactive stent is prepared through the steps that I-type rat tail collagen is dissolved in an acetic acid solution, a DME/F12 culture medium and a NaOH solution are added to prepare an I-type rat tail collagen solution, CSCI and beta-sodium glycerophosphate are dissolved in the DME/F12 culture medium to prepare a temperature-sensitive CSCI solution, and the I-type rat tail collagen solution is mixed with the temperature-sensitive CSCI solution. The collagen composite bioactivestent can be used for treating myocardial infarction ischemic heart diseases by being taken as tissue engineered myocardium to be injected into a myocardial ischemia area.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and relates to a bioactive scaffold, in particular to a composite bioactive scaffold based on CSCI (chlorinated chitosan) and collagen, and a preparation method of the bioactive scaffold. The bioactive stent of the invention has temperature sensitivity and is suitable for treating myocardial infarction-like ischemic heart disease. Background technique [0002] Myocardial infarction (MI) is a disease in which coronary artery blockage leads to ischemic necrosis of myocardial cells, which seriously endangers human health. After adult cardiomyocytes are injured and necrotic, they are gradually replaced by fibrous scar tissue. On the one hand, the newly formed fibrous scar leads to ventricular remodeling and heart failure; on the other hand, it leads to increased electrophysiological heterogeneity in the MI region, aggravates the heart failure caused by cardiac asynchronous contraction, an...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/20A61L27/12A61L27/50A61L27/52A61L27/54A61L27/58
CPCA61L27/12A61L27/20A61L27/24A61L27/50A61L27/52A61L27/54A61L27/58A61L2300/602A61L2400/06A61L2430/20C08L5/08
Inventor 左琳陈思宇李燕楠颉丽英曹济民申晶
Owner SHANXI MEDICAL UNIV
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