47 results about "Cell Microenvironment" patented technology

This invention relates to a method for amplifying neural stem cells in vitro by 3-dimensional culture. The method comprises: selecting microcarrier with 3-dimensional environment, pre-treating, coating the microcarrier with 40-60 ng/mL alkaline fibroblast growth factor, 40-60 ng/mL epidermal growth factor, and B27 DMEM/F12 neural stem cell serum-free culture medium, adding 1X105-1X106 neural stem cells into the culture bottle, taking out the microcarrier grown with neural stem cells, removing the microcarrier, and rinsing cells to obtain neural stem cells. The porous microcarrier can enlarge the culture area. The alkaline fibroblast growth factor and epidermal growth factor can promote cell multiple fission and improve cell microenvironment, which is advantageous for multiple fission of neural stem cells.

The invention relates to an isolated organ preserving device and a preserving method thereof, and belongs to the technical field of medical equipment. The isolated organ preserving device is structurally characterized in that continuous mechanical irrigation and membrane oxygenation are adopted, an isolated organ continuously obtains nutrients and the oxygen supply, a cell metabolite is removed in time, and a cell microenvironment is better maintained, so that the function normality is ensured. The isolated organ preserving device disclosed by the invention can be used for directly contacting the organ perfusate and the organ perfusion instrument by adopting a disposable consumable material sterilized through epoxyethane, thereby meeting the requirement for sterility of a system. Besides, the isolated organ preserving device disclosed by the invention can be used for judging the consumption of the nutrients contained in the organ perfusate and the gathering degree of metabolic wastes by monitoring the pH value of the organ perfusate in real time and can be used for abandoning the organ perfusate after a certain standard is achieved and replacing the fresh organ perfusate, thereby further ensuring the possibility of long-time preservation; the isolated organ preserving device can be used for evaluating the damage condition of the isolated organ by monitoring the front and back perfusion pressure of the isolated organ in real time and converting the perfusion resistance by combining a flow number.

The invention relates to the use of Epidermal Growth Factor (EGF) in a preferably-injectable pharmaceutical composition which is administered by means of infiltration into and around chronic cutaneous ischaemic lesions in order to prevent diabetic foot amputation. Said composition can be administered to recently-created surgical surfaces damaged by the effect of acute reperfusion with oxygenated blood following prolonged ischaemia, thereby preventing further surgical procedures and favouring the preservation of the extremity. The aforementioned composition can be used to improve (i) the cell microenvironment, thereby increasing the reparative and defensive capacity and viability of the is tissues and (ii) the cicatrisation of cutaneous ischaemic lesions, thereby stimulating cell proliferation. The invention is suitable for use in human, veterinary and experimental medicine, specifically in vascular angiology and surgery, dermatology, burn treatment and reconstructive surgery and geriatric medicine. Said composition can be used for recalcitrant ulcers which are associated with lesions in the macro and/or microvasculature, patients with inadequate lymphatic and/or venous return and ulcers or other lesions which are difficult to cicatrise and/or heal.

The invention provides a rhodamine fluorescent molecular probe using a quinoline derivative as an identification group and a synthesis method thereof, and relates to a rhodamine fluorescent molecular probe and a synthesis method thereof, solving the problem that the existing rhodamine fluorescent probe molecules based on the quinoline derivative identification group are few. A structural formula of a fluorescent molecular probe is as shown in specifications. A preparation method comprises the following steps: 1, preparing reaction liquid; and 2, adjusting the pH value of the reaction liquid, carrying out spin-drying and performing silica gel column chromatographic separation to obtain the rhodamine fluorescent molecular probe using the quinoline derivative as the identification group. A structural formula of another fluorescent molecular probe is as shown in specifications. A preparation method comprises the following steps: 1, preparing an intermediate rhodamine B hydrazide; and 2, dissolving the intermediate rhodamine B hydrazide and a quinoline-2-aldehyde derivative into absolute ethanol, refluxing under the protection of nitrogen, carrying out spin-drying and performing silica gel column chromatographic separation to obtain the rhodamine fluorescent molecular probe using the quinoline derivative as the identification group. The rhodamine fluorescent molecular probe disclosed by the invention is applied to the field of fluorescent imaging detection of metal ions in biological tissues and cell microenvironments.

The invention belongs to the technical field of biology, relates to environment-sensitive tumor-targeting polymer micelle and a preparation method thereof, and especially relates to a cell microenvironment-sensitive oxidized ascorbic acid-modified tumor-targeting polymer micelle administration system and a preparation method thereof. An administration system realizes delivery in tumor by a glucose transporter mechanism, the polymer micelle of which the inner core has a disulfide bond crosslinked barrier is designed by use of the GSH characteristics of high density in the cell microenvironment, drug release characteristics trigged by the cell microenvironment are obtained, and the environment-sensitive tumor-targeting polymer micelle has a high transport capability, a fast transport rate and difficult saturation. Compared with polypeptide protein molecules, the environment-sensitive tumor-targeting polymer micelle has the characteristics of simple synthesis preparation, high stability and no immunogenicity. Through synergism of targeting and structure stabilization, drug delivery to a target position and a target cell is realized so that drug delivery efficiency is greatly improved and thus the environment-sensitive tumor-targeting polymer micelle has a good clinical application prospect.

The invention provides a preparation method of a cell nutrient solution for promoting hair regeneration. The preparation method mainly comprises three steps: 1) primary isolation of ADSCs (adipose-derived stem cells); 2) enlarged cultivation of the ADSCs; and 3) induced culture of the ADSCs. The cell nutrient solution prepared by the invention can solve the problem of alopecia from the point of cell regeneration; the ADSCs-conditioned medium (the nutrient solution), in a periodic hair follicle reconstruction process, can activate a stem cell microenvironment of hair follicle and can regulate and control the normal growth of the hair follicle; by conducting low-dose UVB radiation, ADSCs survival, migration and paracrine capacities can be significantly improved, and an angiogenesis capacity and a hair regeneration capacity can be also obviously improved; by cultivating the ADSCs with a fibroblast-conditioned medium, cell survival and metabolism processes in a body environment can be simulated, and the secretion of more cell factors, which are conducive to cell microenvironment regulation and control, from cells can be promoted; and meanwhile, the cell nutrient solution prepared by the method is convenient in cell preparation, broad in source and convenient for clinical popularization, and cells can be derived from fat wastes obtained from clinical liposuction and skin grafts removed in other surgical operations.

A theranostic composition and method for determining the cell-specific potency of drugs is provided. Further, compositions and methods useful for studying the therapeutic profile of one or more drugs within a cell microenvironment, including but not limited to a tumor, are provided.

The invention relates to a preparation method of a composite gel three-dimensional tumor model bracket, comprising the following steps: grinding and drying chitosan, and then dissolving it in an aqueous solution of glacial acetic acid to obtain a chitosan solution; Bubbles; under stirring conditions, add the treated chitosan solution to the silk fibroin aqueous solution, and then add a crosslinking agent to obtain a mixed solution; under stirring conditions, add n-butanol to the mixed solution to obtain a gel; Exclude the air bubbles in the gel, shape and freeze-dry to obtain a composite gel three-dimensional tumor model scaffold. In the present invention, silk fibroin is used as the main material, a small amount of chitosan is used as the auxiliary material, and n-butanol is used as the denaturant to construct tumor tissue engineering scaffold materials in an aqueous solution system, and the prepared composite gel three-dimensional tumor model scaffold is both It can retain the material structure basis of the cell microenvironment in vivo, and can simulate more realistic physiological conditions for tumor cell growth.

The invention discloses a preparation method of a 3D tissue engineering cell ring. By utilizing a relatively narrow growth space and a surface non-adhesion characteristic of a culture mold, added cells are self-assembled into a ring in a culture solution for promoting cell aggregation and leave the surface of a cell culture medium, and a macroscopic tissue block or organoid with a three-dimensional structure is formed. A naturally-formed extracellular matrix exists between the cells prepared by the method, the exogenous scaffold interference does not exist, and the communication and the information exchange between the cells are more complete and direct so as to reduce the difference between a culture environment and an in-vivo environment and more truly simulate an in-vivo cell growth environment. Moreover, the prepared cell ring is a cell body with a regular annular structure, can simulate the in-vivo cell microenvironment, links a 2D cell culture system with the research of tissuesand organs, and is beneficial to research of organoids.

The invention provides a preparation method for mesenchymal stem cell cytokines. The preparation method comprises the following steps: (1) extraction of mesenchymal stem cells; (2) culture; (3) continued culture under low-oxygen conditions; (4) digestion with a 0.25% pancreatin-EDTA solution; (5) centrifugation and cleaning with 0.9% normal saline; (6) adjustment of cell concentration with 0.9% normal saline and addition of EDTA with a concentration of 1 to 3 mg/mL and Vc with a concentration of 5 to 10 mg/mL; (7) freezing-thawing; and (8) centrifugation and filtering. The mesenchymal stem cell cytokines prepared in the invention can effectively overcome the problems that artificially prepared cytokine compositions in the prior art cannot simulate the variety and ratio of cytokines in a cell microenvironment and incur adverse reaction during usage.

The invention discloses a system and a method for constructing an injected three-dimensional cell microenvironment based on microfrozen gel. A microfrozen gel three-dimensional microenvironment vector is made of the frozen gel and in micro scale size. Experiments demonstrate that the prepared microfrozen gel three-dimensional microenvironment vector has the following characteristics that mechanical properties are good; whole morphology can be kept after being injected; the vector can load cells effectively; compared with conventional large-scale frozen gel, the microfrozen gel three-dimensional microenvironment vector can absorb the cells uniformly and has good material transmission performance; the microfrozen gel three-dimensional microenvironment vector has a protection effect for loaded cells in an injection process; and the microfrozen gel loaded with the cells can be injected into an animal body at specific sites and keep original functions and activities in in-vivo cells. The microfrozen gel three-dimensional microenvironment vector plays an important role in the field of cell injection therapy, and has wide application prospects in animal model establishment and the drug screening field.

The invention discloses a 3D voxel printing device based on cell soft spheres and a method thereof. The device comprises a water phase input part, an oil phase input part and a microfluidic microtube,wherein the water phase input part inputs an extracellular matrix material loaded with cells, namely bio-ink, into the microfluidic microtube; and the oil phase input part synchronously inputs a low-viscosity and low-boiling-point oil phase meeting set requirements into the microfluidic microtube. The extracellular matrix material loaded with the cells is separated into monodisperse microdropletsembedded with the cells through an oil phase, the microdroplets are gelled into cell soft spheres in the flowing process in the microtube, the cell soft spheres are sequentially shot onto a substrateby utilizing rapid expansion and volatilization of the oil phase at an outlet of the microfluidic microtube, and the cell soft spheres are adhered together through an incompletely gelled ink material. An in-vitro model capable of highly reducing the human organ or tissue structure and the cell microenvironment can be printed under the condition that a printing material does not contain a biological inert material for assistance.

The invention relates to a cell microenvironment regulating chip device based on groove liquid drop capturing and belongs to the technical field of microfluidics. The device comprises a main solid structure, inlets, groove capturing structures of different transverse-longitudinal ratios, a main channel structure, an outlet and a lower bottom plate and can regulate cell microenvironments. The device has the advantages that the requirements of different experiment conditions are satisfied by the different transverse-longitudinal ratios, experiment costs are saved, and experiments are facilitated; the necking-down design of the groove inlets can change shear stress in the grooves, and the regulation of the living environments of different kinds of cells is benefited; the defect that a traditional device is complex and difficult to operate, and the device is simple in structure and capable of increasing experiment precision and increasing experiment scheme flexibility; the device can facilitate high-precision cell microenvironment regulation and is of great significance to the scientific researches of biological medicine.

The present invention relates to compositions comprising a polymeric matrix or a gel containing functional enzymes capable of re-creating under culture conditions the cell microenvironment existing invivo. The present invention also relates to devices for cell cultures comprising such compositions, in particular hydrogel and the use thereof to control the chemical microenvironment of a cell culture or mimic physiological or pathological conditions of the in vivo cells. The compositions and the devices described herein could be also used in vitro for evaluating the therapeutic effect of a compound on a determined cell line or on primary cells.

The present invention discloses a method for reducing plant cell browning risk through absorption of quinone substances with silica gel. The method comprises: preparing a silica gel absorption material having a specific shape, and sterilizing so as to be spare; during a cell culture process, completely contacting the silica gel absorption material and a plant cell culture, wherein the color of the silica gel absorption material is changed into red-brown after absorbing quinone substances; and taking out the silica gel absorption material after the absorption achieves a saturation state or the culture is completed. According to the present invention, the silica gel is selected as the absorption agent for the cell browning related quinone substances so as to avoid absorption on cell nutrients in the culture medium, reduce the content of the quinone substances in the cell microenvironment, avoid plant (Taxus chinensis) cell browning death, and easily increase stability and cell activity of plant (Taxus chinensis) cell culture; and browning generally causes the decreased cell metabolism level, such that the secondary metabolite having drug activity in the cells can be increased by using silica gel absorption to avoid browning, wherein the yield of the anticancer drug paclitaxel in the Taxus chinensis cells can be increased.

According to the vascularization bone cell sheet scaffold complex and the preparation method thereof, a method for co-culturing osteoblast cells and angiogenic cells is adopted, through a cell sheet technology, the loss of extracellular matrixes caused by a traditional cell digestion technology is eliminated, the original cell microenvironment is reserved, and the cell sheet scaffold complex has the advantages that the cell sheet scaffold complex is simple in structure and convenient to use. Through the synergistic effect of osteogenesis and vascularization, the survival and outcome of osteoblasts in the bone tissue engineering scaffold are facilitated; furthermore, the organic combination of the 3D printing bone tissue engineering scaffold and the cell sheet solves the problems that a pure cell sheet is low in strength and cannot maintain an osteogenesis space.

The invention discloses a preparation method of composite hydrogel, and a construction method of a cell microenvironment bionic system. The preparation method of the composite hydrogel comprises the following steps: mixing a gelatin solution, a sodium alginate solution, a glycerin solution, a glutaraldehyde solution, a phosphate buffer salt solution and a sterile aqueous solution according to a preset volume ratio to obtain a precursor solution the glycerol volume concentration being 1-5%; freezing the precursor solution to convert gelatin from sol to gel, and freezing glycerol to form pores; and adding a calcium chloride solution to carry out ionic crosslinking on sodium alginate in the gel to obtain the composite hydrogel, wherein the concentration of calcium chloride is associated with the rigidity of the composite hydrogel. The composite hydrogel prepared by the embodiment of the invention is good in forming, stable in mechanical property and uniform in pore structure, and can be used for simulating an extracellular matrix microenvironment in vitro.

The invention relates to a biochemical detection instrument. The invention aims to provide a rapid, accurate and automatic detection system for realizing the trace volume of a large number of ROS content samples aiming at cell, tissue and organ microenvironments such as pleural fluid, peritoneal fluid, cerebrospinal fluid, amniotic fluid, follicular fluid, cell culture fluid and the like. The technical scheme is as follows: the active oxygen content automatic detection system suitable for a cell microenvironment is characterized in that the automatic detection system comprises a sample transmission reaction system, a detection system, a washing system communicated with the sample transmission reaction system through a water pipe pipeline and a purging system communicated with the sample transmission reaction system through an air pipe pipeline, wherein the sample transmission reaction system and the detection system are sequentially communicated through a light shielding pipeline; thesample transmission reaction system comprises a sample injector and a DCFH supply bin which are connected in parallel and then communicated with a reaction bin through a light shielding pipeline; andsample injection valves are respectively arranged between the sample injector and the reaction bin and between the DCFH supply bin and the reaction bin.

The invention discloses a dynamic cell microenvironment simulation platform and a preparation method thereof. The platform takes hydrogel as a cell culture carrier, and can selectively regulate and control the combination and dissociation of one or more ligands in the hydrogel and a cross-linked network structure of the hydrogel in real time, wherein the ligands are proteins and polypeptides with specific structures determined according to the types of cells and receptor types expressed by the cells, and are modified in the hydrogel based on a DNA hybridization principle, so that a dynamic cell microenvironment is simulated in vitro, and the platform can be used for regulating and controlling the interaction between the cells and the microenvironment. The platform is simple in preparation method, easy to operate and good in biocompatibility, and meanwhile, the physical and chemical properties in a biological environment are stable.

The invention discloses an Ac-SDKP-LDH nanocrystallization preparation as well as a preparation method and application thereof. The Ac-SDKP-LDH nanocrystallization preparation comprises LDH and Ac-SDKP inserted between LDH layers and is prepared through the following steps that the LDH and the Ac-SDKP are fully mixed and react at the temperature of 2-8 DEG C for 8-24 h, the Ac-SDKP is inserted between the LDH layers through ion exchange, and the Ac-SDKP-LDH nanocrystallization preparation is obtained. The Ac-SDKP-LDH nanocrystallization preparation disclosed by the invention can be used for preparing anti-silicofibrosis medicines. The method for preparing the Ac-SDKP-LDH nanocrystallization preparation is simple and easy to operate, and according to the prepared Ac-SDKP-LDH nanocrystallization preparation, under the action of a cell microenvironment, a cationic laminate of LDH dissolves in inorganic metal ions, so that the Ac-SDKP is released, the effect of protecting the Ac-SDKP is achieved, the anti-EMT effect can be sufficiently exerted, and the Ac-SDKP-LDH nanocrystallization preparation is used for preventing and treating silicosis fibrosis and has the medicine controlled release characteristics of long lasting time, high biological safety and pH sensitivity. In addition, the LDH has a certain anti-fibrosis effect, and the anti-fibrosis effect of the LDH is enhanced by compounding the LDH and the Ac-SDKP.