9 results about "Cell Microenvironment" patented technology

Disclosed in the present invention are a biomimetic vascularized iPSC-hep spheroid for liver regeneration, and a preparation method therefor. The biomimetic vascularized iPSC-hep spheroid is a microcapsule with a porous hyaluronic acid methacryloyl as a shell and a human-induced pluripotent stem-cell-derived hepatocyte spheroid as an inner phase, and human umbilical vein endothelial cells adhere to the surface of the shell. The biomimetic vascularized iPSC-hep spheroid of the present invention promotes effective communication between hiPSC-heps and HUVECs, and provides barrier protection for an encapsulated hiPSC-hep spheroid so as to avoid attack by the immune system. Compared with dispersed cells, the cell-to-cell interaction in the cell spheroid improves cell-to-cell communication, helps to simulate the physiological heterogeneous structure and cell microenvironment of the liver, and can more effectively repair an acutely failing liver.

The invention provides responsive release polypeptide hydrogel. The hydrogel comprises a gel product of a raw material composition containing the following raw material components: (A) a biocompatible polymer containing at least two sulfydryl groups; (B) a matrix metalloproteinase substrate polypeptide; (C) a compound containing a phenolic hydroxyl group structure; (D) a cross-linking agent; and (E) water; the sequence of the matrix metalloproteinase substrate polypeptide is at least one of the following sequences (b1) to (b2): (b1) Cys-Pro-Leu-Gly-Val-Arg-Gly-Arg-Gly-Asp-Ser, (b2) Cys-Pro-Leu-Gly-Val-Arg-Gly-Asp-Ser, (b3) Cys-Pro-Leu-Gly-Val-Arg-Gly (b2), Dnp-Pro-Leu-Gly-Met-Trp-Ser-Arg-C-ys, and (b3), Dnp-Pro-Leu-Gly- The responsive release polypeptide hydrogel can release polypeptide in a cell microenvironment to promote tissue regeneration and repair, and can be used for preparing muscle-bone healing promoting materials and treating ligament injury diseases such as rotator cuff injury.

The invention discloses a method for low-density amplification of T cells and an obtained product, and belongs to the technical field of cell culture. The method comprises the following steps: activating a cell population containing T cells, and co-culturing with a lentiviral vector carrying a target gene for transduction; the method comprises the following steps: transduction is carried out on cells, then the transduction cells are inoculated into a shake flask at an inoculation density of not higher than 2 * 10 < 5 > cells / mL, shake culture is carried out to realize amplification, and amplification of 30-120 times can be realized. According to the method, a low-density cell microenvironment is optimized through dynamic oscillation, the problems of signal insufficiency, factor dilution and metabolism imbalance are solved, the efficiency bottleneck of traditional low-density amplification is broken through, and the method is suitable for large-scale production. The method has the beneficial effects of simplicity in operation, high cell product viability, high purity, low cost, wide scale adaptability and the like. The invention also provides a T cell product prepared by the method.

This invention provides a cell microenvironment stabilizer that captures hydrogen ions, its preparation method, and its application, belonging to the field of biomedical technology. The preparation method of this cell microenvironment stabilizer includes the following steps: (1) mineralizing CaCl2 with TGF-β1, catalase, and polyethylene glycol-polyglutamic acid block copolymer to obtain mineralized nanoparticles; (2) preparing hydrogel microspheres under freezing conditions using a microfluidic device, and then photocrosslinking the frozen hydrogel microspheres; (3) mixing the hydrogel microspheres with the mineralized nanoparticles to prepare the cell microenvironment stabilizer. This invention prepares a mineralized hydrogel microsphere, which, as a cell microenvironment stabilizer, can be used to block the NLRP3 cascade in chronic inflammation, capturing excess hydrogen ions through the mineralization layer to neutralize the acidic microenvironment. This micro / nano bioreactor based on hydrogel microspheres can effectively interfere with NLRP3 in the microenvironment of degenerated tissues from inside and outside the cells, inhibiting chronic inflammation.

The invention discloses a bionic heterogeneous tissue engineering meniscus stent and a preparation method thereof, and discloses application of the bionic heterogeneous tissue engineering meniscus stent in the field of meniscus repair. Based on a multi-nozzle 3D printing technology, a biodegradable high polymer material and bio-ink loaded with growth factors / stem cells are innovatively adopted, and the highly bionic heterogeneous tissue engineering meniscus is constructed. Specifically, degradable macromolecular polycaprolactone (PCL) is used as a raw material, and annular and radial fibers are printed to form a stent frame by accurately regulating and controlling a printing path and simulating a natural meniscus collagenous fiber distribution rule; on the basis, biological ink loaded with differentiated growth factors and stem cells is printed in a staggered manner according to different physiological characteristics of a red region and a white region of the meniscus, so that partitioned precise inoculation of seed cells and controllable partitioned release of the growth factors are realized, and a cell microenvironment with spatial specificity is provided for meniscus regeneration; the tissue repair efficiency and the bionic integrating degree are obviously improved.

The invention belongs to the technical field of biological medicine, and particularly relates to application of farnesol in preparation of a medicine for resisting senescence of mesenchymal stem cells. The invention discloses farnesol as a traditional Chinese medicine monomer for the first time, which can effectively slow down the aging of adriamycin-induced adipose-derived stem cells in vitro, and can be used for preparing the anti-aging medicines for the mesenchymal stem cells. The farnesol can be used for removing aged adipose-derived stem cells and maintaining the steady state of a cell microenvironment. After treatment with 2 [mu] M farnesol for 24 h, key senescence markers such as P53 and P21 in adriamycin-induced senescence adipose-derived stem cells are significantly reduced, which indicates that farnesol can down-regulate senescence signal pathways in cells.

The application discloses a 3D bioprinted radial porous scaffold for staged bone regeneration and a preparation method thereof, the 3D bioprinted radial porous scaffold can provide a biomimetic cell microenvironment to support the infiltration of host repair cells to a defect site, and promote bone integration after implantation, in addition, the sequential release of double growth factors and the sustained release of PO4 3‑ and Ca 2+ in the 3D bioprinted radial porous scaffold can complete the three key steps of staged bone regeneration, can realize efficient bone regeneration, and represents a promising biomimetic staged regeneration strategy.

The present application relates to the technical field of skin care products, and in particular to a stem cell composition for improving aging and metabolic dullness and a preparation method thereof.The composition is compounded by an animal umbilical cord extract, a schizosaccharomyces pombe fermentation product lysate, DNA sodium, a tripeptide-105 and a cold orchid extract, and the five components synergistically regulate, accelerate melanin metabolism, reduce melanin deposition, inhibit melanin production, stimulate cell regeneration, replace old and worn-out cells, repair damaged cells, enhance cell vitality, inhibit inflammatory response, calm the cell microenvironment, and achieve the best long-acting lightening, whitening, barrier repair, soothing irritation, and improvement of severe acne clinical effects in terms of efficacy.The composition not only has faster and longer-lasting effects, but also has good safety and applicability, and has a broad market application prospect.

The invention relates to a double-network hydrogel. The double-network hydrogel is constructed from an animal acellular extracellular matrix and polypeptide. The invention discloses extraction of animal acellular extracellular matrix, and a method and application of the animal acellular extracellular matrix and polypeptide to construct the dual-network hydrogel. The preparation method comprises the following steps: cleaning and crushing animal tissues, carrying out decellularization treatment by using sodium chloride and urea to obtain an extracellular matrix of the animal tissues, removing other components through dialysis, and compounding the extracellular matrix with polypeptide hydrogel to form an interlaced dual-network scaffold through mutual response. The scaffold structure provides an effective support environment for cell three-dimensional culture, realizes optimization of cell three-dimensional microenvironment construction, and can be applied to cell culture and organoid culture.