35 results about "Biochemical mechanism" patented technology

The invention belongs to the technical field of plant cultivation and breeding and discloses a method for screening a variety with the highest nitrogen use ratio from various cut-flower chrysanthemum varieties, wherein the method comprises the following steps: a) acquiring a water planting seedling; b) performing the sandy culturing primary screening: dividing the water planting seedling into two groups, performing the low-nitrogen treatment on one group while performing the normal nitrogen treatment on the other group, respectively measuring the screening indexes of each variety containing the relative dry weight, relative nitrogen content and relative chlorophyll content, and then screening 2 to 6 varieties with higher nitrogen use ratio and 2 to 6 varieties with lower nitrogen use ratio for the purpose of performing comparison; and c) performing the nutrient solution cultivating repeated screening: cultivating the varieties screened from the step b) in the nutrient solution, and repeatedly screening one variety with the highest nitrogen use ratio and one variety with the lowest nitrogen use ratio. The cut-flower chrysanthemum variety which is finally screened by using the method is used for further improving the existing cut-flower chrysanthemum variety and providing the material for researching the biological characteristics and the physiologic biochemical system of the cut-flower chrysanthemum variety.

The present invention provides compositions comprising randomized in-frame fusion polynucleotides and methods for introducing them into a host organism to identify desirable phenotypic changes that disrupt or alter existing genetic or biochemical mechanisms or pathways, thus creating novel characteristics of the transformed organism. Methods for using the compositions for increasing diversity within populations of organisms are also presented.

The invention belongs to the technical field of plant cultivation and breeding and discloses a forcing method of the German iris. The method includes: a, collecting a plant; b, treating the plant, namely soaking roots of the plant in 10% acetone solution containing 600mg / L gibberellin for 24h, wrapping the roots with absorbent paper after soaking, and placing the plant in cold storage in a refrigerator at 4 DEG C; c, transplanting a seedling, namely preparing culture medium composed of nutrient soil, garden soil and sand in the ratio of 2:3:1, preparing a planting pot, taking the treated seedling out of the refrigerator, planting the seedling in the pot, fully watering the seedling, placing outdoors to ease the seedling for 3 days, and moving the seedling to a greenhouse; d, topdressing, namely dressing with bean cake fertilizer, containing 3-4kg / m<3> water, after the seedling is kept in the greenhouse for four weeks; and e, observing the blooming time. The German iris cultured by the method blooms nearly two months early, and basis for further modification on the varieties of the German iris and search on biological characteristics and physiological and biochemical mechanisms of the German iris is provided.

Provided is a microorganism modification filling coal mining method. The microorganism modification filling coal mining method includes the steps that filling materials on the ground are calcificatedby microorganisms, the calcificated filling materials are transported to a goaf to be compacted, the goaf is filled with the calcificated filling materials, then, microorganism grouting is conducted on a filling body, and after coal mining within each step is completed, microorganism grouting filling is carried out. The microorganism modification filling coal mining method is easy to implement andenvironmentally friendly; by the adoption of microorganism filling, the compressive strength of the filling body is effectively improved; a new trend is provided for biochemical-mechanism-based microorganism filling coal mining technologies under deep high-stress conditions.

The invention relates to a cow's penis peptide extraction method, which comprises steps of cleaning, digesting, discharging soup, hydrolyzing, filtering, concentrating and spray-drying. With the application of the extraction method disclosed by the invention, problems on the effective absorption and utilization of macromolecular protein in human body are effectively solved, and micromolecular transformation and extraction of the protein are achieved. Rich tissue protein in cow's penis is transformed into micromolecular peptide through directional biological enzymolysis; the protein, which is derived from an animal body, is quite close to human body in structure and function, and the protein, which has a micromolecular structure, can be directly absorbed by intestines without digestion and can rapidly participate in normal metabolism and regulation of the human body; therefore, the protein, in pharmacology, is higher in biochemical mechanism reasonability and specific treatment effectiveness. The cow's penis peptide has the characteristics of being strongly targeted, free from toxic and side effects, significant in curative effect, high in nutritive value, easy to to be absorbed by human body and the like.

The invention discloses a novel prothetic group connecting arm for synthesizing diubiquitin and a synthesis method of the diubiquitin. The prothetic group connecting arm disclosed by the invention is loaded on protein through TEC (Triethyl Citrate) reaction and then is coupled with first-section ubiquitin through NCL; finally, a prothetic group is removed to obtain a ubiquitin chain based on a ubiquitin expression unit. According to the method disclosed by the invention, the disadvantage of a former technology that macromolecules are subjected to coupling reaction through TEC and the efficiency is low are overcome, and small molecules and the macromolecules are subjected to efficient reaction; a formed amide bond is closet to a natural isopeptide bond, so that a subsequent biochemical mechanism research is convenient to perform.

The invention relates to the technical field of bio-fertilizers, and particularly relates to a preparation method of an agarwood balsam-based bio-fertilizer. The preparation method comprises the stepsof preparing agarwood balsam-based biological fluid, staking and fermenting, overturning and fermenting for the second time, post-fermenting for the third time, and proliferating agarwood balsam biological bacteria. According to the preparation method, the agarwood with balsam is used to cultivate balsam biological flora, the prepared bio-fertilizer is prepared from plentiful balsam biological flora and related derivative physiological transformation substances and enzymes, so that the derivative biochemical mechanism of a half-post is enhanced, the number of biological bacteria of proliferated Fusarium, hypocrea jecorina and Rhizomucor variabilis is increased greatly, the biochemical process in the plant and precipitation of active substances of the plant are enhanced, endogenous fungi is spread more rapidly so as to produce balsam at a faster speed, and the produced balsam has higher quality. The bio-fertilizer also has a corrosion resistance.

The present invention describes diagnosis and treatment of antibody-mediated inflammatory auto-immune diseases. The biochemical mechanisms underlying such disorders are described as characteristic molecular markers and antibody-mediated ligand-receptor interactions. Specifically, the activation of T-cells by disease specific IgG binding to the IGF-1 receptor is shown to underlie thyroid associated ophthalmopathy associated with Graves' disease and rheumatoid arthritis. Diagnostics for detection of disease are provided, as are therapeutics based on the determination of the mechanisms underlying a particular pathology.

The invention discloses an HPLC detection method of the content of jasmonic acid in a sugarcane leaf. The method comprises the following steps that 1, jasmonic acid is separated out from a sugarcane leaf to be detected; 2, jasmonic acid is subjected to methyl esterification; 3, HPLC is adopted for detection. The method is especially suitable for determination of the content of jasmonic acid in the sugarcane leaf infected with a pokkah boeng disease, interference of pathogenic bacteria of the pokkah boeng disease on a detection result can be effectively eliminated, technical support is provided for research of judging infection and resistance differences of sugarcane varieties for the pokkah boeng disease, and therefore a scientific and powerful technical guarantee is provided for pokkah boeng disease-resistance sugarcane breeding and a jasmonic acid mediated sugarcane pokkah boeng disease pathogenic bacteria stress response biochemical mechanism.

The embodiment of the invention discloses a super-bubble biochemical gas cap segregation apparatus and application thereof. The super-bubble biochemical gas cap segregation apparatus comprises a container, a super-bubble mechanism arranged at the lower part of the container, a gas cap mechanism arranged above the container and a biochemical mechanism arranged between the super-bubble mechanism andthe gas cap mechanism; the super-bubble mechanism comprises a water sump at the lower part of the container and a sprayer communicated with the water sump; the biochemical mechanism comprises a carrier arranged above the water sump, a biochemical filter material arranged on the carrier and probiotics distributed on the biochemical filter material; the gas cap mechanism comprises a gas cap cover arranged above the container, a gas cap cavity is formed between the gas cap cover and the biochemical filter material, the top of the gas cap cavity is provided with a gas cap overflow port, and the bottom of the gas cap cavity is provided with a water outlet. The super-bubble biochemical gas cap segregation apparatus provided by the embodiment of the invention has more economical and more excellent cost-benefit ratio, quicker and more obvious effects, less manpower investment and wider application prospect.

Stannous fluoride polyol solid solutions combine three complimentary, biochemical mechanisms attributed to Sn++, F− and Polyol, respectively to reduce growth and metabolism of Streptococcus mutans while effecting superior Bioactivity Quotients. The stannous fluoride polyol solid solution particulate compositions of the present invention comprise:(a) stannous fluoride at between about 0.01 and about 0.8% by weight;(b) a polyol at between about 0.1 and about 30% by weight;(c) an astringency neutralizer at between about 0.01 and about 0.4% by weight, where the ratio of astringency neutralizer to stannous fluoride is from between about 0.01 and about 0.2;(d) a mucoadhesive at between about 1.5 and about 70% by weight, wherein the ratio of mucoadhesive to stannous fluoride polyol is from between about 7 to 1 and about 25 to 1;(e) a pH stabilizer, selected from the group consisting of malic, fumaric, citric acid and combinations thereof, wherein the ratio of pH stabilizer to stannous fluoride polyol is from between about 0.03 and 5 and preferably from between about 0.1 and about 3; and(f) optional flavorants, stabilizers, preservatives, conditioners, and oral care adjuncts.

The invention provides an oral solution for enhancing immunity and delaying ageing. The ratio of each raw material component for preparing 500 parts of the oral solution by weight is as follows: 1.2 to 1.4 parts of a pig thymus gland extracting solution, 1.2 to 1.4 parts of a sheep placenta extracting solution, 14 to 16 parts of honey, 0.2 to 0.3 part of sodium cyclamate, 0.3 to 0.4 part of citricacid and the balance of purified water. According to the oral solution provided by the invention, pig thymus gland and sheep placenta extracts are combined and processed into a human body health-careproduct for the first time and the product contains abundant small molecular active peptides; the content of polypeptide in the product is greater than or equal to 400 mg / ml; an effective component can rapidly participate in normal metabolism and regulation of human bodies; the oral solution has higher biochemical mechanism rationality and human body health-care effectiveness aiming at immunity enhancement and ageing delaying in the aspect of pharmacology; the oral solution has the characteristics of strong regulation pertinence, high nutrient value, easiness in absorption by human bodies andno toxic and side effect.

The invention relates to environmental-friendly special wastewater treatment equipment and in particular relates to zero-emission medical wastewater treatment equipment and method. The treatment equipment comprises a strong pretreatment mechanism, a biochemical mechanism and an advanced treatment mechanism connected in sequence, wherein the biochemical mechanism comprises a first treatment area and a second treatment area communicated with each other; a plurality of elastic packing layers are arranged in the first treatment area from top to bottom; each elastic packing layer is composed of a plurality of elastic packing chains; each elastic packing chain is formed by connecting a plurality of pieces of elastic packing in sequence; at least one bend is arranged on the elastic packing chain;and impellers are arranged at the bottom and side faces of the first treatment area. Compared with the prior art, the environmental-friendly special wastewater treatment equipment disclosed by the invention has the advantages that the packing is wholly stacked and sways along an S-shaped curve, the packing is orderly slowly impelled in a circular curve in the process, problems of violent wire fracture, drop and the like are solved, the sludge can be uniformly stirred and dispersed, biogas can be effectively dispersed, and rapid reproduction growth of novel activated sludge is promoted.

The invention discloses a method for promoting, controlling and cultivating Iris Hybrids Louisiana in target flowering time and belongs to the technical field of plant cultivation and breeding. The method specifically comprises the following steps: (1) seedling raising: selecting a two-year raw Iris Hybrids Louisiana plant, fertilizing and raising; (2) low-temperature treatment: carrying out low-temperature treatment on the plant and a basin on the Tianmu Mountain; (3) flower forcing in a greenhouse: after low-temperature treatment for 40 days, carrying out flower forcing in the greenhouse; (4) fertilizer and water management: carrying out conventional daily management, watering once a week and applying compound fertilizers once a month; (5) flowering observation: observing and calculating the flowering time. The method has the benefits that Iris Hybrids Louisiana is finally enabled to flower about three months in advance, and a reference is provided for further carrying out flowering time regulation and control on existing Iris Hybrids Louisiana varieties and researching the biological characteristics and the physiological and biochemical mechanisms of the existing Iris Hybrids Louisiana varieties. The method can be popularized and applied to Iris Hybrids Louisiana large-scale production.

The invention discloses an HPLC determination method for the gibberellin content in sugarcane leaves. The HPLC detection method comprises the following steps: (S1) adding liquid nitrogen into to-be-detected sugarcane leaves, grinding, carrying out methanol extraction and centrifugation, extracting supernate, extracting residues with methanol, extracting supernate after the centrifugation, combining the types of supernate, carrying out reduced pressure vaporization, carrying out petroleum ether extraction and decoloration, carrying out reduced pressure steaming on a low-layer water phase, adding a flow phase to dissolve, carrying out volume fixation, and filtering to obtain liquid as a sample solution for later use; and (S2) determining the gibberellin content in the sample solution by virtue of an HPLC. By virtue of the HPLC determination method, the gibberellin content in the sugarcane leaves can be determined, and the scientific basis is provided for the research of a biochemical mechanism of the gibberellin in the mediation of the stress response of pathogenic bacteria of the top rot of the sugarcanes.

The invention discloses an HPLC detection method of the content of salicylic acid in sugarcane leaves. The method comprises the following steps: S1, adding the sugarcane leaves to be detected into liquid nitrogen, grinding the leaves, carrying out methanol extraction, centrifuging the obtained extract, taking obtained supernatant, carrying out methanol extraction on obtained residues, centrifuging the residue extract, taking obtained supernatant, mixing the supernatants, carrying out reduced pressure evaporation, carrying out extraction decolorizing, carrying out reduced pressure evaporation on obtained lower layer water phase, adding a mobile phase to dissolve the evaporated water phase, allowing the volume of the obtained solution to reach a constant value, and filtering the solution to obtain a sample solution for later use; and 2, detecting the content of salicylic acid in the sample solution through adopting HPLC. The method allows the content of salicylic acid in sugarcane leaves to be accurately detected, and provides a scientific and powerful detection technology for the top rot resistance difference research of sugarcane varieties and the stress response biochemical mechanism of salicylic acid mediated sugarcane top rot pathogens.

A microbial modification filling-based coal mining method, comprising the operation steps of performing ground microbial calcification on a filling material, conveying the calcified filling material to a goaf for tamping and filling, and then performing microbial grouting on a filling body, where microbial grouting and filling is performed after each step of coal mining is completed. By means of microbial filling, the compressive strength of the filling body is effectively improved, environment-friendliness is realized, and the biochemical mechanism-based microbial filling-based coal mining technology in a high-stress deep part is taken to a new direction.

The invention discloses an application of AHNAK in inhibiting generation of drug resistance of EGFR-TKI drugs in HCC. It is found that the AHNAK protein is a key substance for activating the drug resistance of HCC in EGFR-TKI drugs, and the drug resistance of HCC can be reduced by blocking the expression of the AHNAK protein in vivo, so that a better liver cancer treatment effect is achieved. The invention further researches the biochemical mechanism of the AHNAK protein participating in the activation of the IGF-1R pathway, and provides a theoretical basis for further screening of drugs for resisting HCC drug resistance.

The invention discloses a method for promoting tartary buckwheat seed germination and seedling root development, and belongs to the technical field of plant cultivation. The method comprises the following steps: accelerating germination: tartary buckwheat seeds are soaked and disinfected with 0.1% thiophanate-methyl, cleaned with clear water and then placed in an environment at the temperature of 15-25 DEG C, and the seeds are soaked with clear water for 6 h; seed sowing: the germinated tartary buckwheat seeds are subjected to germination disc water culture through a graphene oxide UP water suspension at the environment temperature of 15-25 DEG C, and the solution is added in time according to the evaporation capacity until the solution does not exceed 1 / 3 of the height of the seeds, wherein after the water culture treatment through the graphene oxide, the germination rate and germination potential of the tartary buckwheat are obviously improved; bud seedling transplanting; seedling sampling. The method has the advantages of being easy to operate, low in cost, remarkable in effect and the like, and a new thought and a new method can be provided for tartary buckwheat field production practice and research in the fields related to physiological and biochemical mechanisms such as root-crown relation, material transportation, breeding, and stress resistance; compared with a common cultivation method, root systems are easy to separate and less in winding, root system damage in the sampling process is effectively reduced, and the integrity of the root systems is guaranteed, so that the corresponding experimental research is facilitated.

The invention relates to the technical field of garbage treatment, and particularly discloses environment-friendly garbage treatment equipment which comprises a standard garbage container, a feeding mechanism, a crushing mechanism, a dehydration mechanism, a biochemical mechanism, a purification mechanism and a rack. The feeding mechanism, the smashing mechanism, the dewatering mechanism and the biochemical mechanism are all fixed to the rack. The purification mechanism is fixed at the top of the rack; the standard garbage container is arranged on the feeding mechanism, the feeding mechanism is arranged on one side of the smashing mechanism, the dewatering mechanism is arranged below the smashing mechanism, one side of the dewatering mechanism is connected with the biochemical mechanism, and the biochemical mechanism is connected with the purifying mechanism through a pipeline. According to the method, the biochemical degradation technology is utilized from the source for immediate on-site treatment, garbage decay is limited, secondary pollution in the kitchen garbage transportation process is avoided, garbage resource utilization and clean emission are achieved through high-intelligence operation, and environmental protection is facilitated.

The invention discloses a HPLC detection method for the malic acid content of a sugarcane leaf. The method comprises the following steps: S1, adding a to-be-detected sugarcane leaf into liquid nitrogen for trituration, then adding an aqueous methanol solution with a concentration of 70 to 90%, carrying out grinding to obtain slurry, carrying out extraction at 2 to 5 DEG C, then carrying out ultrasonic extraction and centrifugation, taking a supernatant, adding the aqueous methanol solution with a concentration of 70 to 90% into residues for ultrasonic extraction, carrying out centrifugation, taking another supernatant, repeating extraction and centrifugation of the residues once to three times, combining all the supernatants, fixing the combined supernatants to a certain volume with methanol with a concentration of 70 to 90% and carrying out filtering; and S2, detecting the content of malic acid in a sample solution by using HPLC. The method can accurately detect the malic acid content of the sugarcane leaf and provides scientific and powerful detection technology for research on difference of sugarcane varieties in inductive reactance to top rot and biochemical mechanism of stress response to pathogens of malic acid-mediated top rot of sugarcane.

The invention provides a chitin-containing compound fertilizer and a preparation method thereof. By a double-liquid dimolecular ammoniation process, chitin and a compound fertilizer are perfectly chelated. The prepared chitin-containing compound fertilizer has the characteristics of pest control, disease resistance, dual fertilizer and pesticide supplementation, high efficacy and the like; the chitin-containing compound fertilizer can be used as a natural soil structure modifier, and has the effects of strengthening roots and seedlings, resisting diseases and adverse conditions, inhibiting nematodes and activating and enhancing physiological and biochemical mechanisms of crops.