9 results about "Biochemical mechanism" patented technology

The invention provides a technical condition prediction method for a tunnel civil engineering structure, and relates to the technical field of traffic control monitoring systems.A biological acoustic composite sensing system comprising a distributed optical fiber acoustic sensing system and microorganism sample collection and analysis is constructed, and acoustic depth mode characteristics are mined by applying a deep learning model; the microbial dynamic biomarker is analyzed and identified by using bioinformatics; multi-modal information, a domain knowledge graph and a causal inference algorithm are fused, and a dynamic causal network model for revealing an internal driving relation in the degradation process is constructed; further, training and applying an adaptive neural network prediction model based on causal driving and fusing physical and biochemical mechanism constraints, and carrying out probabilistic prediction on future technical conditions of the tunnel; and executing anti-fact reasoning by using the causal network and the prediction model, and generating and optimizing an intervention strategy of cooperation of physical measures and biochemical measures. According to the method, the accuracy and advance of tunnel structure state prediction can be remarkably improved.

The present application relates to the technical field of biological medicine materials, and particularly relates to a magnesium chelated vitamin C nanomaterial, a preparation method and application thereof. The preparation raw material of the nanomaterial comprises VCMg, a lipid modification material and a solvent I. The preparation raw material of the VCMg comprises an oil phase emulsion and an aqueous phase emulsion. The oil phase emulsion contains a magnesium source, a non-ionic surfactant, a stabilizer and a solvent II. The aqueous phase emulsion contains vitamin C, a dispersing agent and water. The research results of the present application establish that the VMNP is a unique nanometer platform integrating coordination chemistry and biological regulation, reveal a new biochemical mechanism of stabilizing unstable nutrients through materials, promote the anti-fibrosis treatment based on vitamin C, and provide a new technical idea for the cross research of nanotherapy and molecular biology mechanism.

The invention discloses a droplet micro-fluidic device for cell co-culture and a use method, belongs to the field of micro-fluidic analysis, and particularly relates to a droplet micro-fluidic device which comprises a chip main body material, an oily liquid substance covering the surface of the chip main body material, and a vertical porous membrane material arranged in the oily liquid substance. The porous membrane material comprises at least one of a polycarbonate porous membrane, a PVDF (Polyvinylidene Fluoride) porous membrane and a porous composite membrane, and the porosity of the porous membrane material is more than 1%. The droplet microfluidic device has the advantages of high flux, low consumption, multi-step operation, direct observation and the like, and has strong compatibility with a classical biochemical mechanism analysis method. The liquid drops are separated by the porous membrane material and are connected with each other, and the liquid drops on the two sides can be used as a cell, organoid or tissue culture chamber and also can be used as a liquid storage tank for sample adding. The liquid drops are used as a culture chamber, so that co-culture of cells can be realized, and support is provided for cell co-culture, drug screening and cell microenvironment simulation.

The invention relates to a method for noninvasively evaluating age-dependent metabolic characteristics of mouse oocytes. The method comprises the following steps: S1, collecting oocytes of mice at different month ages; s2, treating the oocytes to obtain a sample capable of being used for spontaneous Raman spectroscopy and imaging; s3, performing spontaneous Raman spectroscopy and imaging of different oocytes; s4, processing and analyzing Raman spectrum data; s5, processing and analyzing two-dimensional and three-dimensional Raman imaging; s6, analyzing morphological and spectral characteristics of oocytes of mice of different age groups; and S7: determining the specific age-related change of the lipid content and distribution, the cytochrome c level and the metabolic profile in the mouse oocytes. Compared with the prior art, the method has the advantages that the metabolic change of the oocytes of mice with different maternal ages is analyzed by Raman spectrum for the first time, the age-dependent distribution change of lipid and cytochrome c is revealed through high-resolution subcellular mapping, and a new research scheme is provided for the biochemical mechanism of oocyte senescence.

The invention discloses an anti-embolism and anti-scouring medical hemostatic material, and relates to the technical field of medical supplies. According to the invention, the nano material in the components provides an ultra-large specific surface area, so that the blood coagulation factor XII can be instantly activated, and the fastest endogenous blood coagulation pathway in the human body can be directly started; various ions such as calcium and magnesium which are synergistically released jointly strengthen the blood coagulation cascade reaction and the blood clot strength, active, rapid and multi-target hemostasis of a biochemical mechanism is realized, and the effect is far better than that of a traditional material depending on physical adsorption; efficient hemostatic components are firmly loaded on the microspheres with the particle size larger than 100 micrometers through an original technology, the outer layer is covered with a layer of membranoid matter with the surface gap being 2-3 micrometers, and the risk that particles enter blood circulation through blood vessel crevasses is fundamentally eradicated from the physical size; the product is instantly hydrated and adhered on the wound surface to form a firm blood scab-microsphere composite barrier. The product is instantly hydrated and adhered on the wound surface to form a firm blood scab-microsphere composite barrier. The barrier can effectively resist impact of arterial blood pressure and is not prone to being washed off, and therefore the barrier is excellent in performance in high-pressure jet bleeding scenes of cardiac surgery, vascular surgery and the like.

The Inventors developed conditions allowing to efficiently detect differences in cardiomyocytes relaxation phases associated with increased cardiomyocytes stiffness. They used a library of patient-specific human induced pluripotent stem cells (hiPSC) either from healthy donors or carrying mutations (i.e., MYH7 and BRAF mutations) associated with hypertrophic cardiomyopathy, a condition typically associated with impaired diastolic function as well as an increase in cardiomyocytes passive stiffness. They performed a high throughput screening on hiPSC-derived cardiac cells to identify microRNAs capable of modifying the relaxation rates of cardiomyocytes. In particular, they set up a large-scale functional genomics using miRNAs screening. All identified miRNAs were tested for their impact on cardiac cells movement and calcium transient. miRNAs with the highest impact were in particular tested on ECTs and changes in diastolic function, were measured and compared to the results obtained at the cell level. They manipulated the most interesting ‘hits’ in 3D models using similar readouts as in primary assays. They tested the impact of the positive ‘hits’ in mechanical models (developed during the exploratory part) and establish physiological and biochemical mechanisms of action of the identified key proteins. They finally identified two promising miRNAs that could be used for improving striated myocytes relaxation and, more generally, to alleviate symptoms related to striated muscle stiffness, in particular in the context of heart failure with a preserved ejection fraction (HFpEF).

The field of intracellular condensates is an emerging and rapidly growing field. Liquid membrane-less organelles composed of intrinsically disordered proteins (IDPs) are implicated in many critical functions of the cell, including signaling and metabolic pathways, transcription, translation, and the cell cycle. However, understanding of their basic biological and biochemical mechanisms is still limited by a lack of high-throughput methods to identify their protein constituents. The yeast-liquid-hybrid (YLH) method disclosed herein, inspired by the classical yeast-two-hybrid (Y2H) screen, can facilitate high-throughput identification of proteins that partition into protein condensates (e.g., liquid, gel-like, or solid condensates) in living cells. The development of this method will allow for the discovery of protein interactions at a much larger scale than currently possible, and the ability to screen large chemical collections to identify drug candidates to treat diseases associated with pathological protein condensates.

The application provides a high-activity Millettia pachycarpa Benth. low-temperature controlled enzyme vacuum freeze-dried tablet preparation process, and belongs to the technical field of deep processing of Chinese medicinal materials with the same functions of food and medicine.The application realizes the complete blocking of endogenous enzyme browning and lipid oxidation rancidity of Millettia pachycarpa Benth. from the biochemical mechanism level through the synergistic coupling of the exclusive process system of moderate temperature and humidity directional enzyme blunting, low-temperature constant-temperature physical color protection, double-stage gradient pre-freezing, multi-section variable-temperature vacuum freeze-drying, constant-temperature softening, and grading screening, maximizes the retention of the heat-sensitive natural active ingredients and the original flavor of Millettia pachycarpa Benth. such as polysaccharide, flavone and triterpene saponin under the premise of zero chemical color protection additives. The whole process parameters of the application are accurately controllable, standardized and repeatable for mass production, and the industry pain points of the existing Millettia pachycarpa Benth. processing, such as serious browning, large loss of active ingredients, flavor deterioration, unstable batches and short shelf life, are solved. The application is suitable for industrialized continuous production line production, can realize the high-quality, standardized and large-scale in-depth processing of the Lingnan native Millettia pachycarpa Benth., and helps the upgrading of the regional characteristic South China medicine industry.

This disclosure relates to a sophisticated method for protecting pome and stone fruit trees from freezing and subfreezing temperatures. The method involves applying a specialized Coating Composite Material (CCM), which can be used alone or in conjunction with other polymers, fertilizers, plant growth regulators (PGRs), and cryoprotectants, directly to the trees from the bud swell stage to full bloom. The protective CCM is primarily formulated from sodium alginate and calcium chloride, which together form a biodegradable film around plant tissues, providing the first layer of protection against frost. This advanced coating methodology not only forms a physical barrier against frost, but also enhances plant resilience to cold stress through a variety of biochemical mechanisms, offering a comprehensive solution to protect vulnerable fruit trees in changing climatic conditions.