A kind of hplc detection method of jasmonic acid content in sugarcane leaves

A detection method and technology of sugarcane leaves, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that there is no detection method for the content of jasmonic acid in leaves of sugarcane tip rot, and achieve complete, repeatable and accurate extraction of active ingredients The effect of good sex and high purity

Inactive Publication Date: 2019-04-26
SUGARCANE RES INST GUANGXI ACADEMY OF AGRI SCI
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Problems solved by technology

However, there is no efficient and accurate detection method for the content of jasmonic acid in leaves of sugarcane tip rot.

Method used

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  • A kind of hplc detection method of jasmonic acid content in sugarcane leaves
  • A kind of hplc detection method of jasmonic acid content in sugarcane leaves
  • A kind of hplc detection method of jasmonic acid content in sugarcane leaves

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Embodiment 1

[0029] The HPLC detection method of jasmonic acid content in the sugarcane leaf of embodiment 1

[0030] S1. Mix acetone and 50mmol / L citric acid aqueous solution at a volume ratio of 7:3 to prepare an extraction solvent, pre-cool the extraction solvent to 4°C, select sugarcane leaves infected with the pathogen of sugarcane tip rot as the sample to be tested, and take the sample to be tested Add 10mL of pre-cooled extraction solvent to 2g of the sample, then add liquid nitrogen, grind into a slurry, mix well, add 10ml ethyl acetate, shake for 3min, and then extract ultrasonically for 30min, centrifuge at 8000 r / min for 10min, and take the supernatant, Add 3ml of pre-cooled extraction solvent to the residue, ultrasonically extract for 30min, centrifuge at 8000 r / min for 10min, take the supernatant, combine the two supernatants into another 50ml centrifuge tube, add graphitized carbon black and PSA silica gel respectively for adsorption 0.6g of each agent, shaken for 5min, centr...

Embodiment 2

[0046] Example 2 Judgment of the difference in susceptibility and resistance of sugarcane varieties infected with tip rot

[0047] (1) Preparation of inoculum: Inoculate the pathogenic bacteria of sugarcane tip rot on PDA medium for 3 days to activate, pick the mycelia from the edge of PDA medium and inoculate them in sterilized potato glucose water medium for 3 days, centrifuge, Use sterilized absorbent cotton to filter and collect the obtained bacteria, and use sterile water and bacteria to prepare a concentration of 1 × 10 6 CFU / ml sugarcane tip rot pathogen spore suspension.

[0048] (2) Treatment of inoculation materials: sugarcane variety YT94-128 and sugarcane variety GT37 were selected as inoculation materials, and 30 barrels of each variety were planted under greenhouse conditions, with 4 buds per barrel, and the seeds were soaked with 0.3% carbendazim for 20 minutes before planting. Manage normally after emergence, and inoculate when 5 to 6 complete leaves grow.

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Abstract

The invention discloses an HPLC detection method of the content of jasmonic acid in a sugarcane leaf. The method comprises the following steps that 1, jasmonic acid is separated out from a sugarcane leaf to be detected; 2, jasmonic acid is subjected to methyl esterification; 3, HPLC is adopted for detection. The method is especially suitable for determination of the content of jasmonic acid in the sugarcane leaf infected with a pokkah boeng disease, interference of pathogenic bacteria of the pokkah boeng disease on a detection result can be effectively eliminated, technical support is provided for research of judging infection and resistance differences of sugarcane varieties for the pokkah boeng disease, and therefore a scientific and powerful technical guarantee is provided for pokkah boeng disease-resistance sugarcane breeding and a jasmonic acid mediated sugarcane pokkah boeng disease pathogenic bacteria stress response biochemical mechanism.

Description

technical field [0001] The invention relates to the technical field of detection of sugarcane endogenous hormones, in particular to an HPLC detection method for the content of jasmonic acid in sugarcane leaves. Background technique [0002] sugar cane( Saccharum officinarum ) is the most important sugar crop in my country, and sucrose accounts for 92% of my country's total sugar production. The phytohormones contained in sugarcane are involved in the physiological regulation of almost all processes of its growth and development, from cell growth, division and differentiation, to seed dormancy, fruit development, sex differentiation and aging processes. For sugarcane, it has two very important indicators that directly affect the economic value of sugarcane, namely, sugar content and yield. However, if sugarcane is affected by diseases and insect pests and cannot be detected in time, it will greatly affect the sugar content and yield of sugarcane. If it is possible to determ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 王泽平林善海蒋洪涛林波周珊何为中李毅杰罗霆杨翠芳韦金菊高轶静张革民刘璐段维兴
Owner SUGARCANE RES INST GUANGXI ACADEMY OF AGRI SCI
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