Cell Laboratory Observation System Based on Harness Light

Abstract

The present invention provides a cell laboratory observation system based on beam bundle light, which relates to the technical field of analysis and processing. It includes a non-linear beam self-fusion generator, a quantum entanglement state modulation network, a cell microenvironment response matrix, and a super-topological light field reconstruction unit for super-resolution observation and dynamic intervention of cells. Through the non-linear beam self-fusion technology, a self-focusing beam is generated by using a non-linear crystal doped with rare earth ions, and the focusing depth is dynamically adjusted by combining with a liquid crystal modulator, achieving sub-micron-level focusing without traditional lenses. The quantum entanglement state modulation network breaks through the optical diffraction limit through the non-local characteristics of entangled photon pairs. In addition, the chiral vortex beam generated by the super-topological light field reconstruction unit maintains high intensity and high resolution in deep tissues, enabling researchers to clearly observe fine structures such as neuron synapses or myocardial cell gap junctions in deep tissues, providing a high-precision tool for studying complex biological systems.

Description

Technical Field

[0001] The present invention relates to the technical field of analysis and processing, and specifically to a cell laboratory observation system based on bundle light. Background Art

[0002] According to a cell observation system disclosed in Chinese Patent Publication No. "CN117686427A", in order to comprehensively and effectively detect target cells in an operating space such as a clean bench, the cell observation system of the present invention includes: a first photographing device disposed in a culture vessel, having a first photographing unit for obtaining a first image of cells in the culture container; a second photographing device disposed outside the culture vessel, having a second photographing unit for obtaining a second image of the culture container after being taken out of the culture vessel; a processing device connected to the first photographing device and the second photographing device; and a display device for displaying at least a part of the first image and the second image. The processing device extracts target cells from the first image, calculates and stores the positions where the extracted target cells exist, and superimposes and displays the positions where the target cells exist in the display representing the culture container on the display device.

[0003] According to a method for establishing a spectral response function of an observer's cone cells disclosed in Chinese Patent Publication No. "CN109084898B", by selecting display devices with different primary color spectra, based on five color centers recommended by the CIE, color-normal observers are organized to conduct cross-media color matching experiments, calculate the CIELAB color difference values between the target color and the matching color, use the Monte Carlo method to generate eight different physiological and physical parameters, combine them with the observer's age and the viewing field angle, generate n groups of cone cell response functions of different individual observers, substitute them into the spectral data of the target color and the matching color in the color matching experiment, calculate n groups of CIELAB color difference values, compare the two groups of CIELAB color difference values, and use the color matching function corresponding to the smaller color difference value as the color matching function of the observer, thereby establishing the spectral response function of the cone cells of a color-normal individual observer. The present invention magnifies the color discrimination difference of the observer and helps to quantitatively describe the metamerism phenomenon of the observer.

[0004] The above patent documents and the prior art have the following technical problems when in use:

[0005] Problem 1: Traditional fluorescence microscopes and confocal microscopes are limited by the diffraction limit (resolution is only 200 - 300 pixels / μm) and shallow penetration depth (100 - 500 μm), making it difficult to simultaneously achieve high resolution and deep tissue observation, resulting in insufficient analysis of deep cell behavior;

[0006] Problem 2: The traditional observation system lacks a real-time feedback mechanism, and imaging and intervention are separated (e.g., confocal microscopes are only 30 frames per second with an intervention accuracy of 2 micrometers), making it impossible to dynamically adapt to cell changes and resulting in insufficient control over dynamic processes.

[0007] Problem 3: Traditional technologies (such as fluorescence microscopes and confocal microscopes) have single functions, require switching between multiple devices, are complex to operate and inefficient, and are difficult to meet the requirements of modern biology for high-throughput and multi-dimensional data. Summary of the Invention

[0008] Technical Problems to be Solved

[0009] In view of the deficiencies of the prior art, the present invention provides a cell laboratory observation system based on a light beam bundle, which solves the following problems:

[0010] 1. Aiming at the problem that traditional cell observation devices have limited observation capabilities, it is difficult to simultaneously achieve high-resolution and deep tissue observation, resulting in insufficient analysis of deep cell behavior;

[0011] 2. Aiming at the problem of lacking a real-time feedback mechanism, the separation of imaging and intervention, and the inability to dynamically adapt to cell changes, resulting in insufficient control over dynamic processes;

[0012] 3. Aiming at the problem of single engineering equipment and insufficient adaptation ability, which cannot meet the multi-channel and multi-dimensional data processing requirements.

[0013] Technical Solutions

[0014] To achieve the above objectives, the present invention is realized through the following technical solutions: A cell laboratory observation system based on a light beam bundle, the system includes a non-linear beam self-fusion generator, a quantum entanglement state modulation network, a cell microenvironment response matrix, and a super-topological light field reconstruction unit, for super-resolution observation and dynamic intervention of cells, where:

[0015] The non-linear beam self-fusion generator uses non-linear optical media to generate a beam with a self-focusing effect. This beam forms a micrometer-level focusing hot spot through an adaptive refractive index gradient during propagation, performs dynamic convergence of the beam, and adjusts the focusing depth and intensity through a periodically oscillating polarization state for the observation of different cell layers;

[0016] The quantum entanglement state modulation network consists of a group of entangled photon pairs, and performs ultrafast modulation on the phase and polarization of the beam through the non-local correlation between photons to generate an interference pattern with spatial adaptability. This pattern self-adjusts the resolution according to the real-time scattering characteristics of the cells, breaking through the traditional optical diffraction limit;

[0017] The cell microenvironment response matrix is composed of an embedded photosensitive polymer and a nanoscale acoustic wave sensor, which can sense the chemical gradient, mechanical stress, and temperature changes in the cell microenvironment in real time, convert these signals into beam modulation parameters, form a feedback closed loop coupled with the cell state, and precisely intervene in cell behavior;

[0018] The super-topological optical field reconstruction unit reconstructs the optical field distribution by using the principle of topological photonics and the singularity characteristics of the non-Hermitian system to generate a vortex beam with a non-linear propagation trajectory. This beam maintains high intensity and high resolution in deep tissues, and at the same time, realizes the directional excitation of intracellular molecular activities through the chiral regulation of the optical field.

[0019] Preferably, the non-linear optical medium in the non-linear beam self-fusion generator is a non-linear crystal doped with rare earth ions. The non-linear crystal realizes an adaptive refractive index gradient through the electro-optic effect, and the polarization state of the beam with self-focusing effect is controlled by a liquid crystal modulator integrated on the crystal surface to dynamically adjust the focusing depth.

[0020] Preferably, the quantum entanglement state modulation network includes an entangled photon pair source and a spatial light modulator. The entangled photon pair source generates entangled photons with specific polarization and phase relationships, and the spatial light modulator dynamically adjusts the interference pattern of the photon pairs according to the feedback signal of the cell microenvironment response matrix to adaptively compensate for the cell scattering characteristics.

[0021] Preferably, the embedded photosensitive polymer in the cell microenvironment response matrix is a photochromic polymer, which is used to change the refractive index under illumination of a specific wavelength. The nanoscale acoustic wave sensor is composed of a piezoelectric nanowire array and is used to detect the mechanical vibration of cells. The cell microenvironment response matrix converts the sensed signals into beam modulation parameters through an embedded microprocessor.

[0022] Preferably, the super-topological optical field reconstruction unit adopts a photonic crystal structure based on a non-Hermitian system. Topologically protected edge states are designed in the photonic crystal structure to generate a vortex beam with chiral characteristics. The orbital angular momentum of the vortex beam is adjusted by an external magnetic field for the directional excitation of intracellular molecular activities.

[0023] Preferably, the non-linear beam self-fusion generator and the quantum entanglement state modulation network are connected by an optical fiber coupler, and the optical fiber coupler multiplexes the self-fusion beam and the entangled photon pairs to form a composite beam. The composite beam realizes both focusing and interference modulation during propagation to improve the imaging resolution and stimulation accuracy.

[0024] Preferably, the system further includes an adaptive optics module. The adaptive optics module uses a wavefront sensor and a deformable mirror to correct the wavefront aberration of the light beam in real time according to the interference pattern generated by the quantum entanglement state modulation network, so as to improve the imaging of deep tissues.

[0025] Preferably, the cell microenvironment response matrix and the super-topological optical field reconstruction unit are connected by a feedback control loop, and the feedback control loop dynamically adjusts the chirality of the vortex beam according to the microenvironment change to specifically regulate the intracellular signal pathway.

[0026] Beneficial effects

[0027] The present invention provides a cell laboratory observation system based on a light bundle. It has the following beneficial effects:

[0028] 1. The system of the present invention uses the nonlinear beam self-focusing technology to generate self-focusing beams by using a nonlinear crystal doped with rare-earth ions, and combines a liquid crystal modulator to dynamically adjust the focusing depth. It can achieve sub-micron-level focusing without traditional lenses. At the same time, the quantum entanglement state modulation network breaks through the optical diffraction limit through the non-local characteristics of entangled photon pairs, reaching a super-resolution imaging ability of 500 pixels / μm. In addition, the chiral vortex beam generated by the super-topological optical field reconstruction unit maintains high intensity and high resolution in deep tissues, and the penetration depth can reach 1000 μm, far exceeding the 100 - 500 μm of traditional technologies, enabling researchers to clearly observe fine structures such as neuron synapses or myocardial cell gap junctions in deep tissues, providing a high-precision tool for studying complex biological systems.

[0029] 2. The system of the present invention uses the cell microenvironment response matrix to use a photochromic polymer and a piezoelectric nanowire array to sense chemical gradients, mechanical stress, and temperature changes in real time, and an embedded microprocessor converts the signals into beam modulation parameters; the feedback control loop dynamically adjusts the chirality and focusing depth of the vortex beam to form a closed-loop mechanism that is coupled with the cell state in real time. The adaptive regulation supports a real-time frame rate of 100 frames / second and an intervention accuracy of 0.5 μm, enabling researchers to precisely intervene in cell behavior, such as optimizing cell synchrony in cardiac tissue engineering or regulating photosynthesis efficiency in plant cell research, providing a new path for personalized medicine and agricultural production.

[0030] 3. The present invention integrates nonlinear optics, quantum physics, and topological photonics. The fiber coupler multiplexes self-focusing beams and entangled photon pairs, the adaptive optics module corrects wavefront aberrations, and the software platform coordinates data analysis and parameter optimization to realize the collaborative operation of super-resolution imaging and dynamic intervention. The integrated design significantly improves the experimental efficiency and supports high-throughput screening and multi-task research, such as quickly analyzing the dynamics of protein aggregation in neurodegenerative diseases, promoting the rapid development of biomedical research. Brief Description of the Drawings

[0031] Figure 1 is the system architecture diagram of the present invention;

[0032] Figure 2 is the system operation step diagram of the present invention;

[0033] Figure 3 is the relationship diagram of the focusing depth and polarization state of the self-focusing beam of the present invention;

[0034] Figure 4 is the modulation effect diagram of the interference pattern of the entangled photon pairs of the present invention;

[0035] Figure 5 is the dynamic response diagram of the cell microenvironment signal and the beam modulation parameters of the present invention;

[0036] Figure 6 is the orbital angular momentum and chirality characteristic diagram of the vortex beam of the present invention;

[0037] Figure 7 is the performance comparison diagram between the new system of the present invention and the traditional technology;

[0038] Figure 8 is the system transmission relationship diagram of the present invention. Detailed Description of the Preferred Embodiments

[0039] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention. Specific Embodiment 1:

[0041] As Figures 1 to 8 shown, a cell laboratory observation system based on a bundle of light beams, the system includes a non-linear beam self-fusion generator, a quantum entanglement state modulation network, a cell microenvironment response matrix, and a super-topological optical field reconstruction unit, for super-resolution observation and dynamic intervention of cells, wherein:

[0042] The non-linear beam self-fusion generator uses a non-linear optical medium to generate a beam with a self-focusing effect. The beam forms a micron-level focusing hot spot through an adaptive refractive index gradient during propagation, performs dynamic convergence of the beam, and adjusts the focusing depth and intensity through a periodically oscillating polarization state for observation of different cell layers;

[0043] The quantum entanglement state modulation network consists of a set of entangled photon pairs, which can perform ultrafast modulation on the phase and polarization of light beams through the non-local correlation between photons, generating an interference pattern with spatial adaptability. This pattern self-adjusts the resolution according to the real-time scattering characteristics of cells, breaking through the traditional optical diffraction limit;

[0044] The cell microenvironment response matrix is composed of an embedded photosensitive polymer and a nanoscale acoustic wave sensor, which can sense the chemical gradient, mechanical stress, and temperature changes in the cell microenvironment in real time, and convert these signals into light beam modulation parameters, forming a feedback closed-loop coupled with the cell state for precise intervention in cell behavior;

[0045] The super-topological optical field reconstruction unit uses the principle of topological photonics to reconstruct the optical field distribution by utilizing the singularity characteristics of a non-Hermitian system, generating a vortex beam with a non-linear propagation trajectory. This beam maintains high intensity and high resolution in deep tissues, and at the same time, realizes the directional excitation of intracellular molecular activities through the chiral regulation of the optical field.

[0046] The system further includes the following:

[0047] The nonlinear optical medium in the nonlinear beam self-fusion generator is a nonlinear crystal doped with rare-earth ions. The nonlinear crystal realizes an adaptive refractive index gradient through the electro-optic effect. The doping of rare-earth ions significantly enhances the nonlinear optical properties of the crystal, enabling it to generate a stronger refractive index change under the action of an external electric field. The enhancement effect stems from the modulation of the crystal's electronic structure by rare-earth ions, which improves the nonlinear polarizability. Moreover, the polarization state of the self-focusing effect beam is controlled by a liquid crystal modulator integrated on the crystal surface for dynamic adjustment of the focusing depth. Adaptive refractive index gradient: Through the electro-optic effect, the externally applied electric field dynamically adjusts the refractive index distribution inside the crystal to form a gradient, causing the light beam to self-focus during propagation, forming a micron-scale focusing hot spot without the need for a traditional lens. The realization of self-focusing depends on the non-linear coupling between the light beam intensity and the refractive index. The refractive index in the high-intensity region increases to further converge the light energy. A liquid crystal modulator is integrated on the crystal surface to dynamically adjust the polarization state of the light beam by changing the orientation of liquid crystal molecules. The change in the polarization state affects the propagation path of the light beam in the crystal, thereby realizing the dynamic adjustment of the focusing depth to meet the observation requirements of cells at different depths. The response time of the liquid crystal modulator can reach the millisecond level, ensuring rapid adaptation to changes in experimental conditions; The crystal material can be selected as potassium tantalum niobate (KTN) doped with neodymium (Nd), which has both a high non-linear coefficient and an electro-optic effect.

[0048] The quantum entanglement state modulation network includes an entangled photon pair source and a spatial light modulator (SLM). The entangled photon pair source generates entangled photons with specific polarization and phase relationships, and uses the non-local characteristics of quantum entanglement to achieve ultrafast modulation. Entangled photon pairs are usually generated in a nonlinear crystal (such as a BBO crystal) through the spontaneous parametric down-conversion (SPDC) process to ensure a high degree of polarization and phase correlation. The spatial light modulator dynamically adjusts the interference pattern of the photon pairs according to the feedback signal of the cell microenvironment response matrix. By loading a phase template, the SLM changes the spatial distribution of the photon pairs to achieve adaptive compensation for the scattering characteristics of cells, effectively reducing the wavefront distortion caused by scattering, thus breaking through the traditional optical diffraction limit and improving the imaging resolution. The resolution of the SLM can reach the megapixel level, supporting the precise generation of complex interference patterns. The modulation frequency of the entangled photon pairs can reach the GHz level, meeting the requirements of real-time imaging and adaptive compensation for the scattering characteristics of cells.

[0049] The cell microenvironment response matrix integrates multi-mode sensing and feedback functions. The embedded photosensitive polymer in the cell microenvironment response matrix is a photochromic polymer, which is used to change the refractive index under specific wavelength illumination to sense the optical signal in the cell microenvironment. Its working principle is based on the photoisomerization reaction, such as the cis-trans isomerization transformation of azobenzene compounds, which can complete the refractive index switching within microseconds. The nano-scale acoustic wave sensor is composed of a piezoelectric nanowire array and is used to detect the mechanical vibration of cells. As a nano-scale acoustic wave sensor, the nanowire array made of piezoelectric materials (such as zinc oxide ZnO) detects the mechanical vibration of cells. The high sensitivity of the array can capture stress changes at the sub-micron level, reflecting the dynamic behavior of cells. The cell microenvironment response matrix converts the sensed signal into beam modulation parameters through an embedded microprocessor. The embedded microprocessor is used to integrate multi-dimensional data such as optical signals and mechanical signals to generate beam modulation parameters. Through real-time algorithm processing (such as Fourier transform or machine learning models), the microprocessor converts the sensed signal into specific modulation instructions to form a feedback closed-loop coupled with the cell state. The response wavelength of the photochromic polymer can be customized (such as 405nm or 532nm), and the density of the piezoelectric array can be optimized to hundreds per square micron to ensure high spatial resolution.

[0050] The super-topological optical field reconstruction unit adopts a photonic crystal structure based on a non-Hermitian system. The photonic crystal structure is designed with topologically protected edge states to generate a vortex beam with chiral characteristics. The orbital angular momentum of the vortex beam is adjusted by an external magnetic field for the directional excitation of intracellular molecular activities. The photonic crystal structure generates a vortex beam with chiral characteristics by means of periodic refractive index modulation and the use of the exceptional point characteristics of the non-Hermitian system. The vortex beam carries orbital angular momentum (OAM), and its phase presents a helical distribution. The orbital angular momentum is adjusted by an externally applied magnetic field, which regulates the boundary conditions of the edge states in the photonic crystal, changes the rotation characteristics of the vortex beam, and realizes the directional excitation of intracellular molecular activities, being applicable to high-precision intervention in deep tissues. The photonic crystal can be fabricated using silicon-based materials, and the topological protection of the edge states ensures the robustness of the beam in scattering media. The magnetic field regulation accuracy can reach the mT level, supporting fine chiral switching.

[0051] The non-linear beam self-fusion generator and the quantum entanglement state modulation network are connected by an optical fiber coupler. The optical fiber coupler multiplexes the self-fusion beam and the entangled photon pairs to form a composite beam. The composite beam simultaneously realizes focusing and interference modulation during propagation for improving imaging resolution and stimulation accuracy. The optical fiber coupler connects the non-linear beam self-fusion generator and the quantum entanglement state modulation network, multiplexes the self-fusion beam and the entangled photon pairs to form a composite beam. The multiplexing process is achieved through wavelength division multiplexing (WDM) or polarization multiplexing to ensure that the independent characteristics of the two beams are retained. The composite beam simultaneously realizes focusing (from the self-fusion beam) and interference modulation (from the entangled photon pairs) during propagation, significantly improving imaging resolution and stimulation accuracy. The optical fiber coupler can adopt low-loss single-mode optical fiber, supporting wide-band (such as 400 - 1000 nm) transmission, and the coupling efficiency is better than 95%.

[0052] The system also includes an adaptive optics module. The adaptive optics module, through a wavefront sensor and a deformable mirror, corrects the wavefront aberration of the beam in real time according to the interference pattern generated by the quantum entanglement state modulation network for improving the imaging of deep tissues. The wavefront sensor adopts a Shack-Hartmann sensor to detect the wavefront aberration of the composite beam and generate a real-time aberration distribution map. The deformable mirror adjusts the mirror shape through micro actuators according to the feedback of the wavefront sensor to correct the aberration, optimize for the interference pattern generated by the quantum entanglement state modulation network, and ensure the imaging quality of deep tissues. The deformation range of the deformable mirror can reach ±10 μm, and the response frequency reaches the kHz level, adapting to rapid wavefront changes.

[0053] The cell microenvironment response matrix and the super-topological optical field reconstruction unit are connected by a feedback control loop. The feedback control loop dynamically adjusts the chirality of the vortex beam according to the microenvironment changes, for the specific regulation of intracellular signaling pathways. The feedback control loop connects the cell microenvironment response matrix and the super-topological optical field reconstruction unit. According to the microenvironment changes (such as optical signals or mechanical stress) sensed by the cell microenvironment response matrix, the loop calculates and adjusts the chirality of the vortex beam through an algorithm; the regulation of chirality is achieved through magnetic fields or phase modulation, and the precise control of chirality adjustment realizes the specific regulation of intracellular signaling pathways, such as activating specific proteins or changing metabolic pathways. The delay of the feedback loop is less than 1 ms, supporting real-time regulation; the regulation algorithm can integrate deep learning models to predict cell responses.

[0054] The above preferred solution constructs an efficient cell observation and intervention system through the deep integration of nonlinear optics, quantum entanglement, topological optical fields, and adaptive technologies. Each component has a refined design in technical details to ensure the excellent performance of the system in super-resolution imaging, deep tissue intervention, and dynamic regulation, providing strong support for biological research and precision medicine. Specific Embodiment 2:

[0056] As Figures 1 to 8 shown, based on the content in the above specific embodiment, the following content is further disclosed:

[0057] The operation steps of the entire system further include the following:

[0058] Sp1: Initialization: System startup and preparation: All core components of the system start simultaneously and enter the working state to lay the foundation for subsequent beam generation, observation, and intervention; the specific process is as follows: The self-focusing beam self-fusion generator uses a nonlinear crystal doped with rare-earth ions to generate a beam with a self-focusing effect, adjusts the crystal refractive index through the electro-optic effect to form an adaptive refractive index gradient, so that the beam forms a micron-level focusing hotspot during propagation. At the same time, the liquid crystal modulator dynamically changes the polarization state of the beam to adjust the focusing depth to prepare for the observation of different cell layers; the quantum entanglement state modulation network starts the entangled photon pair source, generates entangled photon pairs with specific polarization and phase relationships, and prepares to adjust the interference pattern according to the feedback signal through a spatial light modulator (SLM) to achieve ultrafast modulation and super-resolution imaging; the cell microenvironment response matrix starts to monitor the cell microenvironment in real time, uses a photochromic polymer to sense optical signals, a piezoelectric nanowire array to detect mechanical vibrations, and integrates multi-dimensional signals through an embedded microprocessor to provide data for subsequent feedback control; the super-topological optical field reconstruction unit designs topologically protected edge states based on the non-Hermitian system of photonic crystals, and prepares to generate chiral vortex beams by adjusting the orbital angular momentum through an external magnetic field to prepare for deep tissue intervention, ensuring the coordinated initialization of all parts of the system and forming a highly integrated operation foundation;

[0059] Sp2: Beam Generation and Modulation: Optimization of Composite Beams: Generate and optimize composite beams for observation and intervention, involving the collaborative work of multiple components. The specific process is as follows: The self-focusing beam generated by the non-linear beam self-fusion generator and the entangled photon pairs generated by the quantum entanglement state modulation network are multiplexed through an optical fiber coupler. The optical fiber coupling technology combines the two beams to form a composite beam, while retaining the high-precision focusing ability of self-focusing and the interference modulation characteristics of entangled photon pairs to improve imaging resolution and intervention accuracy. Subsequently, the adaptive optics module uses a wavefront sensor to detect the wavefront distortion of the composite beam and dynamically adjusts the mirror shape through a deformable mirror to correct the distortion, ensuring the focusing quality and imaging clarity of the beam in deep tissues. At the same time, the spatial light modulator of the quantum entanglement state modulation network adjusts the interference pattern in real time according to the cell scattering characteristics, and uses the non-local correlation of entangled photon pairs to precisely control the phase and polarization, further breaking through the traditional optical diffraction limit. Through the generation, multiplexing, and modulation of the beam, a high-quality composite beam suitable for cell observation and intervention is formed.

[0060] Sp3: Observation and Intervention: Super-Resolution Imaging and Deep Regulation: Use the modulated composite beam to perform super-resolution observation and deep tissue intervention on cell samples, and combine multiple technologies to achieve high-precision operations. The specific process is as follows: The modulated composite beam is projected onto the cell sample through the system. The dynamic focusing ability of the self-focusing beam enables it to focus on different cell layers to achieve super-resolution imaging, and the interference pattern generated by the entangled photon pairs further improves the resolution, breaks through the diffraction limit, and provides a clear image of the cell structure. At the same time, the super-topological optical field reconstruction unit generates a vortex beam with a non-linear propagation trajectory, uses the topological protected edge states and singular point characteristics of the photonic crystal to maintain the high intensity and high resolution of the beam in deep tissues, and controls the beam chirality by adjusting the orbital angular momentum with an external magnetic field to directionally excite the molecular activities inside the cell, thereby achieving precise intervention in deep tissues. Throughout the process, the optical fiber coupler continuously optimizes the focusing and interference characteristics of the beam, and the adaptive optics module corrects the wavefront distortion to ensure the stability of the observation and intervention effects, seamlessly combining super-resolution imaging and deep intervention to provide strong support for the research of complex biological systems.

[0061] Sp4: Feedback and Adjustment: Dynamically Optimize Beam Characteristics: Dynamically adjust the beam characteristics through a real-time feedback mechanism to adapt to changes in the cellular microenvironment and optimize the observation and intervention effects. The specific process is as follows: The cellular microenvironment response matrix uses photochromic polymers to sense chemical gradient changes, piezoelectric nanowire arrays to detect mechanical stress, and an embedded microprocessor to integrate multi-dimensional signals such as temperature to monitor the state of the cellular microenvironment in real time and convert these signals into beam modulation parameters. These parameters are transmitted to various parts of the system through a feedback control loop. Among them, the super-topological optical field reconstruction unit adjusts the chirality and orbital angular momentum of the vortex beam according to the feedback, the non-linear beam self-fusion generator adjusts the focusing depth through polarization state control, and the quantum entanglement state modulation network optimizes the spatial distribution of the interference pattern, thereby dynamically optimizing the characteristics of the composite beam. The entire adjustment process forms a closed-loop control to ensure the real-time coupling of the beam and the cellular state, improving the accuracy of intervention and the self-adaptability of imaging. Through the collaborative work of sensing, feedback, and adjustment, the system achieves a high degree of dynamic and specific regulation capabilities.

[0062] Sp5: Data Analysis: Result Processing and Parameter Optimization: Analyze the observation and intervention results and optimize the future operating parameters of the system to improve long-term performance. The specific process includes collecting and analyzing imaging data and intervention results through a software platform. Among them, super-resolution imaging data reveals the details of cell structures, and intervention effect data reflects the regulation of molecular activities. The software uses machine learning algorithms to process these data, identify patterns, and optimize the parameters of beam generation, modulation, and feedback control. For example, adjust the polarization state of the self-focusing beam, the refractive index gradient of the non-linear crystal, or the chirality characteristics of the vortex beam. The optimized parameters are stored and applied to the next experiment, forming a cycle of continuous improvement, which not only provides an in-depth understanding of the experimental results but also enhances the self-adaptability and efficiency of the system in a data-driven manner, laying a foundation for future biological research and clinical applications.

[0063] The entire system realizes a complete workflow from system startup to result optimization through five steps: initialization, beam generation and modulation, observation and intervention, feedback and adjustment, and data analysis. With its super-resolution observation and dynamic intervention capabilities, it provides an innovative tool for cell research and precision medicine. Specific Embodiment Three:

[0065] As Figures 1 to 8 shown, based on the content in the above specific embodiments, the following content is further disclosed:

[0066] Compare the system architectures in the above Specific Embodiment One and Specific Embodiment Two with existing cell laboratory observation techniques through experiments. The purpose of this experiment is to demonstrate the advantages of the new system in key performance indicators through specific experimental designs and data comparisons. The specific experimental content is as follows:

[0067] Experimental Objective: The experiment aims to compare the performance differences between the "Cell Laboratory Observation System Based on Harness Light" (hereinafter referred to as the "new system") and traditional fluorescence microscopes and confocal microscopes in cell observation, with a focus on evaluating indicators such as imaging resolution, penetration depth, real-time performance, and intervention precision;

[0068] Experimental Materials: Cell Samples: Neuronal cells cultured in vitro, used to observe deep tissues and dynamic behaviors; Stain: Neuronal cells labeled with GFP (green fluorescent protein);

[0069] Experimental Equipment: New System: Cell Laboratory Observation System Based on Harness Light;

[0070] Traditional Fluorescence Microscope; Confocal Microscope;

[0071] Experimental Design:

[0072] Sample Preparation: Cultivate GFP-labeled neuronal cells under the same conditions to ensure consistent cell status;

[0073] Experimental Conditions: Temperature 37°C; CO2 concentration 5%; Humidity 95%;

[0074] Observation Tasks:

[0075] Task 1: Observe the fine structure on the cell surface and evaluate the imaging resolution;

[0076] Task 2: Observe the cell behaviors in deep tissues and evaluate the penetration depth;

[0077] Task 3: Monitor the cell response to light stimulation in real time and evaluate the real-time performance and intervention precision;

[0078] Data Acquisition For each task, use the three systems to observe and record respectively; Record indicators such as imaging resolution, penetration depth, real-time frame rate, and intervention precision;

[0079] The experimental process is as follows:

[0080] Task 1: Observe the fine structure on the cell surface:

[0081] Steps: Use the new system, traditional fluorescence microscope, and confocal microscope to image the same batch of neuronal cells respectively; Adjust the focal plane to capture the fine structure images of the cell surface; Measure the imaging resolution (in pixels / μm) through image analysis software;

[0082] Objective: Evaluate the resolution ability of the system in observing fine structures;

[0083] Task 2: Observe the cell behaviors in deep tissues:

[0084] Steps: Prepare a multi-layer tissue model from cell samples to simulate the deep tissue environment; Use three systems to image cells in deep tissue respectively; Record the maximum depth (in micrometers) that can be clearly observed;

[0085] Objective: Test the penetration ability of the system in deep tissue observation;

[0086] Task 3: Real-time monitor the response of cells to light stimulation:

[0087] Steps: Use the new system and a confocal microscope (the traditional fluorescence microscope does not have the function of real-time intervention) to perform light stimulation on cells; Record the response of cells to light stimulation in real time; Measure the real-time frame rate (frames per second) and the intervention accuracy (using the light stimulation positioning error as an index, unit: micrometer);

[0088] Objective: Evaluate the performance of the system in dynamic observation and precise intervention;

[0089] Experimental results: The experimental data are summarized in the following table:

[0090] The comparison of imaging resolutions is shown in Table 1:

[0091]

[0092] The comparison of penetration depths is shown in Table 2:

[0093]

[0094] The comparison of real-time performance and intervention accuracy is shown in Table 3:

[0095]

[0096] Analysis of experimental results:

[0097] Imaging resolution: With the quantum entanglement state modulation and super-topological light field reconstruction technology, the new system achieved an imaging resolution of 500 pixels per micrometer, far exceeding the 200 pixels per micrometer of the traditional fluorescence microscope and the 300 pixels per micrometer of the confocal microscope, indicating that the new system has higher clarity in observing the fine structure of cells;

[0098] Penetration depth: Through the non-linear beam self-fusion technology, the new system achieved a penetration depth of 1000 micrometers, significantly better than the 100 micrometers of the traditional fluorescence microscope and the 500 micrometers of the confocal microscope, showing its powerful ability in deep tissue observation;

[0099] Real-time performance and intervention accuracy: The new system supports a real-time frame rate of 100 frames per second and an intervention accuracy of 0.5 microns, far exceeding the 30 frames per second and 2.0 microns of confocal microscopes. Traditional fluorescence microscopes are not applicable due to the lack of real-time intervention function, indicating that the new system has obvious advantages in dynamic monitoring and precise operation;

[0100] The experimental results show that the "Cell Laboratory Observation System Based on Harness Light" is comprehensively superior to traditional fluorescence microscopes and confocal microscopes in terms of imaging resolution, penetration depth, real-time performance, and intervention accuracy. Its advanced technology provides a more efficient and precise observation tool for cell biology research, especially suitable for deep tissue observation and dynamic behavior monitoring. Specific Embodiment 4:

[0102] As Figures 1 to 8 shown, based on the content in the above specific embodiments, the following content is further disclosed:

[0103] When applying the above system structure to actual cell observation applications, the actual effects and application potential of the system of this application are illustrated through cases. The specific cases are as follows:

[0104] Application Case 1: Dynamic Monitoring of Protein Aggregation in Neurodegenerative Diseases:

[0105] When studying neurodegenerative diseases such as Alzheimer's disease, the abnormal aggregation of proteins (such as β-amyloid protein) is one of the core pathological features. However, traditional imaging techniques are difficult to capture its dynamic process in real time. The Cell Laboratory Observation System Based on Harness Light provides a solution to this problem. Researchers applied this system to in vitro cultured neuron models. By using a non-linear beam self-fusion generator to generate self-focusing beams and combining entangled photon pairs generated by a quantum entanglement state modulation network to form composite beams, which were directly projected onto neuron samples, achieving super-resolution imaging at the subcellular level and clearly revealing the details of the formation and distribution of β-amyloid protein aggregates. At the same time, the chiral vortex beams generated by the super-topological light field reconstruction unit were used to directionally excite fluorescently labeled molecules in neurons, enhancing the visualization effect of aggregates. The cell microenvironment response matrix senses the mechanical stress and chemical gradient changes caused by protein aggregation and feeds these signals back to the system to dynamically adjust the focusing depth and chirality of the beams, ensuring the self-adaptability of imaging and intervention. The adaptive optical module continuously corrects wavefront aberrations to ensure the imaging quality of deep tissues. Throughout the process, researchers analyzed the imaging data in real time through a software platform to track the spatio-temporal dynamics of protein aggregation, providing a high-precision tool for revealing disease mechanisms and screening potential drugs.

[0106] Application Case 2: Regulation of Cell Synchronization in Cardiac Tissue Engineering:

[0107] Cardiac tissue engineering requires precise regulation of the synchronous contraction of cardiomyocytes to construct functional cardiac tissue. Traditional methods have limitations in the regulation of deep tissues, and the cell laboratory observation system based on light bundles shows unique advantages in this field. Researchers applied the system to a three-dimensional cardiomyocyte culture system, using a composite beam generated by a non-linear light beam self-fusion generator and a quantum entanglement state modulation network to achieve super-resolution imaging of cardiomyocyte populations, capturing the fine features of cell gap junctions and calcium ion fluctuations. The super-topological light field reconstruction unit uses chiral vortex beams to directionally excite photosensitive ion channels in cardiomyocytes, simulating electrical stimulation signals and inducing cell synchronous contraction. The cell microenvironment response matrix senses the mechanical vibrations and ion concentration changes caused by cell contraction, feeds back to the system to adjust the beam parameters, and optimizes the stimulation frequency and intensity to ensure the accuracy of regulation. The fiber optic coupler and adaptive optical module cooperate to optimize the beam transmission and focusing effects, overcoming the scattering interference in the three-dimensional culture system. Finally, the system analyzes the cell synchrony data through a software platform, providing key support for the construction of physiologically functional cardiac tissue and promoting the clinical transformation of tissue engineering technology.

[0108] Application Case 3: Optimization study on the photosynthesis efficiency of plant cells:

[0109] The photosynthesis efficiency of plant cells directly affects agricultural production, but traditional technologies are difficult to achieve dynamic observation and regulation at the level of living cells. The cell laboratory observation system based on light bundles has brought a breakthrough in this field. Researchers applied the system to a mesophyll cell model. Through the self-focusing beam generated by the non-linear light beam self-fusion generator and the entangled photon pairs of the quantum entanglement state modulation network, a composite beam was formed to achieve super-resolution imaging inside chloroplasts, clearly showing the structure of photosystem II and the light energy transfer process; the chiral vortex beam generated by the super-topological light field reconstruction unit was used to directionally excite photosensitive proteins in chloroplasts, simulating different wavelength illumination conditions to regulate the photosynthesis efficiency; the cell microenvironment response matrix senses the oxygen release and pH changes during photosynthesis, feeds back to the system to dynamically adjust the beam characteristics, and optimizes the imaging and intervention effects; the adaptive optical module corrects the light scattering in plant tissues to ensure the imaging quality of deep cells; throughout the process, researchers analyze the changes in photosynthesis efficiency and metabolites through a software platform, screen out the optimal illumination conditions, and provide a scientific basis for increasing crop yields.

[0110] The above cases demonstrate the practical application value of the cell laboratory observation system based on light bundles in the research of neurodegenerative diseases, cardiac tissue engineering, and plant photosynthesis. Through the integration of non-linear optics, quantum entanglement, and topological light field technologies, the system has achieved a coordinated breakthrough in super-resolution imaging and dynamic intervention, providing an innovative solution for the research and application of complex biological systems.

[0111] It should be noted that in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprising", "including" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or device. Without further limitation, an element defined by the statement "comprising a reference structure" does not exclude the existence of additional identical elements in the process, method, article or device comprising the element.

[0112] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell laboratory observation system based on light of a wire harness, characterized in that: The system includes a non-linear beam self-fusion generator, a quantum entanglement state modulation network, a cell microenvironment response matrix, and a super-topological optical field reconstruction unit for super-resolution observation and dynamic intervention of cells, where: The non-linear beam self-fusion generator uses a non-linear optical medium to generate a beam with a self-focusing effect. This beam forms a micron-scale focusing hot spot through an adaptive refractive index gradient during propagation, performs dynamic convergence of the beam, and adjusts the focusing depth and intensity through a periodically oscillating polarization state for observation of different cell layers. The quantum entanglement state modulation network consists of a set of entangled photon pairs, which perform ultrafast modulation on the phase and polarization of the beam through the non-local correlation between photons to generate an interference pattern with spatial adaptability. This pattern self-adjusts the resolution according to the real-time scattering characteristics of the cells, breaking through the traditional optical diffraction limit. The cell microenvironment response matrix is composed of an embedded photosensitive polymer and a nano-scale acoustic wave sensor, which can real-time sense the chemical gradient, mechanical stress, and temperature changes in the cell microenvironment, and convert these signals into beam modulation parameters to form a feedback closed-loop coupled with the cell state for precise intervention of cell behavior. The super-topological optical field reconstruction unit reconstructs the optical field distribution using the singularity characteristics of a non-Hermitian system through topological photonics principles to generate a vortex beam with a non-linear propagation trajectory. This beam maintains high intensity and high resolution in deep tissues, and at the same time, realizes the directional excitation of intracellular molecular activities through the chiral regulation of the optical field.

2. The cell laboratory observation system based on light of wire harness according to claim 1, wherein: The non-linear optical medium in the non-linear beam self-fusion generator is a non-linear crystal doped with rare-earth ions. The non-linear crystal realizes an adaptive refractive index gradient through the electro-optic effect, and the polarization state of the beam with the self-focusing effect is controlled by a liquid crystal modulator integrated on the crystal surface for dynamic adjustment of the focusing depth.

3. The cell laboratory observation system based on harness light according to claim 1, wherein: The quantum entanglement state modulation network includes an entangled photon pair source and a spatial light modulator. The entangled photon pair source generates entangled photons with specific polarization and phase relationships, and the spatial light modulator dynamically adjusts the interference pattern of the photon pairs according to the feedback signal of the cell microenvironment response matrix for adaptive compensation of the cell scattering characteristics.

4. The cell laboratory observation system based on bundle light according to claim 1, wherein: The embedded photosensitive polymer in the cell microenvironment response matrix is a photochromic polymer used to change the refractive index under illumination at a specific wavelength. The nano-scale acoustic wave sensor is composed of a piezoelectric nanowire array for detecting the mechanical vibration of the cells. The cell microenvironment response matrix converts the sensed signals into beam modulation parameters through an embedded microprocessor.

5. The cell laboratory observation system based on bundle light according to claim 1, wherein: The super-topological optical field reconstruction unit adopts a photonic crystal structure based on a non-Hermitian system. The photonic crystal structure is designed with topologically protected edge states to generate a vortex beam with chiral characteristics. The orbital angular momentum of the vortex beam is adjusted by an external magnetic field for the directional excitation of intracellular molecular activities.

6. The cell laboratory observation system based on harness light according to claim 1, wherein: The non-linear beam self-fusion generator is connected to the quantum entanglement state modulation network through an optical fiber coupler, and the optical fiber coupler multiplexes the self-fusion beam and the entangled photon pairs to form a composite beam. The composite beam simultaneously realizes focusing and interference modulation during propagation, which is used to improve the imaging resolution and stimulation accuracy.

7. The cell laboratory observation system based on bundle light according to claim 1, wherein: The system also includes an adaptive optical module. The adaptive optical module, through a wavefront sensor and a deformable mirror, corrects the wavefront aberration of the beam in real time according to the interference pattern generated by the quantum entanglement state modulation network, which is used to improve the imaging of deep tissues.

8. The cell laboratory observation system based on bundle light according to claim 1, wherein: The cell microenvironment response matrix is connected to the super-topological optical field reconstruction unit through a feedback control loop, and the feedback control loop dynamically adjusts the chirality of the vortex beam according to the microenvironment change, for the specific regulation of the intracellular signal pathway.

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